Training quality improvement is one of the major topics in the education of medical postgraduates.Regular meeting is an effective mode in the training of postgraduates,which can improve the communication between teachers and students,reinforce tutors'teaching consciousness,and standardize the training process.

Placental tissues have many cell types with stem/progenitor cell potential and immunomodulatory properties. Moreover, placental tissue is readily available and easily procured without invasive procedures, and its use does not elicit ethical debate. Thus, placental tissue has been a key focus in studies of searching a new source of human stem/progenitor cells. Most of the research published in this area focuses on mesenchymal stromal cells isolated from various parts of the placenta or epithelial cells isolated from amniotic membrane. Considering the complexity of the placenta, an urgent need exists to define the region of origin and methods of isolation of cells derived from this tissue.

The nuclei of the lymphocytes of rat spleen were isolated with the modified Kohji Ueno method and then were purified. Four kinds of nucleoside triphosphate (ATP, GTP, CTP, UTP) as well as 3H-UTP and certain inorganic ions were added in to study the transcriptional function, DNA-dependent RNA polymerase activity, of the nuclei in vitro. The influence of the substrate concentration, temperature, pH value, the concentration of certain inorganic ions(Mg, Mn, NH4 + ), actinomycin D and ?-amanitin on the polymerase activity was evaluated.It was found that Mn and (NH4)2SO4 in high concentration(0.2M) could markedly enhance the transcriptional activity while actinomycin D and a-amanitin inhibited it. A-amanitin in low concentration (1?g/ml) could inhibit 80% of the transcriptional activity. If proper ion concentration was given, relatively high activity of RNA polymerase could be shown. The transcriptional activity was in linear relation with certain range of the nucleus concentration. Substrate requires the existence of the four kinds of nucleoside triphosphate: this means that the transcriptional model reflects the activity of the DNA-dependent RNA polymerase and is suitable for the study of transcriptional mechanism.

BACKGROUND:Peroxisome proliferator-activated receptor-gamma coactivator-1 alpha (PGC-1α) may play an important role in the exercise-induced skeletal muscle adaptation, which is involved in the regulation of a variety of exercise-induced biological reactions.
OBJECTIVE:To review the PGC-1αand endurance training induced skeletal muscle adaptations.
METHODS:The relevant articles about relationship between PGC-1αand endurance training-induced skeletal muscle adaptations were searched from PubMed database (1995-01/2010-10) by using the keywords of“PGC-1α, skeletal muscle, exercise, mitochondrial biogenesis, adaptations”, and the language was limited to English. Repetitive contents were deleted. The 59 col ected articles were searched. According to the criterion, 37 were classified and sorted.
RESULTS AND CONCLUSION:Endurance training can typical y increase the expression/activity of membrane transporters and mitochondrial metabolic enzymes as wel as increase capil arisation in the skeletal muscle, together enhancing the oxidative capacity of the muscle and the ability to oxidize both carbohydrates and fatty acids. Studies in PGC-1αknockout and overexpression mice have clearly demonstrated that PGC-1αplays an important role in maintaining the expression of mitochondrial metabolic and anti-oxidant enzymes in the skeletal muscle and does influence exercise-induced adaptations of mitochondrial proteins. However, PGC-1αis not exclusively required, and additional factors must be involved in the regulation of both basal expression and exercise-induced adaptations. Exercise-induced PGC-1αexpression and potential y increased PGC-1αactivity are likely the mechanisms contributing to skeletal muscle mitochondrial adaptations and concomitant health beneficial effects of regular physical activity.

Objective To construct the eukaryotic expression vector of mouse 24p3 gene and express it in NIH3T3 fibroblast cell line and verify its biological function of delivering iron into cells. Methods 24p3cDNA was cloned by RT-PCR and the eukaryotic expression vector of mouse 24p3 gene was constructed by gene recombination technique and the recombinant plasmid was verified by restriction enzyme digestion analysis and sequenceing, then transfected into NIH3T3 cells using DOTAP transfection reagent. Then the positive clones were selected and amplified. The expression of 24p3 gene in the culture supernatant of positive clones was analyzed by ELISA and Western blotting and its biological activity of delivering iron into cells was tested by atomic absorption spectrometer. Results The eukaryotic expression vector was constructed successfully. The NIH3T3 cells that stably expressed 24p3 were screened out. 24p3/SIP24 protein could express in the culture supernatant of positive clones and deliver iron into NIH3T3 cells. Conclusion The 24p3 gene was expressed successfully in the transfected NIH3T3 cells with good biological activity, which laid the foundation for further studying its biological function and mechanism involved in the iron metabolism.

Objective: To well understand the mental health status and its affecting factors of the residents aged 15 and above of Weihai city. Methods:The sample was randomly chosen by using cluster-based sampling method and was interviewed by using both self-made questionnaire and Kessler 10 rating scale. SPSS was used to analyze the field data.Results:11652 individuals were interviewed, the score distribution in total was 13.3?5.6 and about 22% of them were likely to have a mild or medium or high mental disorder. The factors affecting mental health status were various, not only including general factors such as age, sex, but also other factors such as physical condition.Conclusion:The factors affecting mental health are multiple, about one in five residents are in poor mental status.

Objective To compare the levels of serum CEA, NSE and CYFRA21-1 of patients with lung cancer and those with benign lung diseases, and to evaluate the clinical differential diagnostic value of combined detection of serum CEA, NSE and CYFRA21-1 in patients with lung cancer. Methods CEA, NSE and CYFRA21-1 were determined with enzyme-linked immunosorbent assay in 122 patients with lung cancer and 37 patients with benign lung diseases. The data was analyzed by t test. Results The serum levels of CEA(12.95?33.04ng/ml), CYFRA21-1(4.87?0.638ng/ml) in patients with lung cancer were significantly higher than that in those with benign lung diseases (1.92?0.84ng/ml and 4.87?0.638ng/ml, respectively)(P0.05). The levels of serum CEA, NSE and CYFRA21-1 were related to types of pathology. The highest levels of CEA, NSE and CYFRA21-1 appeared in adenocarcinoma, small cell carcinoma and squamous cell carcinoma, respectively. The level of serum of NSE (26.05?20.69ng/ml) in patients with small cell carcinoma was significantly higher than that of the patients with benign lung disease, adenocarcinoma (11.26?6.97ng/ml) and squamous cell carcinoma (12.71?7.64ng/ml), respectively (P0.05). Conclusion Combined determination of serum levels of CEA, NSE and CYFRA21-1 is helpful in differential diagnosis of lung cancers.

Finite element analysis (FEA) is broadly used in biomechanics.Being widely used in clinical studies on cervical spine injury,cervical degeneration and stimulating a variety of cervical spine surgeries,cervical finite element model is becoming more and more accurate in recent years.This paper aims to review the development of cervical finite element model,to introduce the application progress of the modeling and analysis in cervical spine injury,cervical disc arthroplasty,cervical interbody fusion,cervical degeneration and cervical instability,and to prospect the foreground of cervical finite element model in future.

Objective The processes responsible for the uptake of very low density lipoprotein(VLDL) by monocyte macrophage cells were investigated. Methods The effects of VLDL concentration, apoE ligand activity and scavenger receptor A (SRA) on the binding of 125 I VLDL to monocyte macrophages were analysed. The influence of VLDL on SRA mRNA and protein expression and VLDL receptor gene translation was probed. Results (1) The differentiated monocyte macrophages induced 125 I VLDL uptake by dose dependent pathway( r =0 71, P

Objective To observe effect of the pulmonary injury and the dynamic changes of the blood gas index on the mortality rate of the dogs suffered from underwater blast injury. Methods 38 adult dogs were brought out underwater blast injury by TNT. The dogs that died on the spot were anatomized immediately, while the survivors were anatomized after 6 hours; the blood samples were taken before the explosions, 30min and 6 hours after them to analyze the blood gas index by i-STAT. Results Most of the lung injuries were pulmonary hemorrhage and edema; the positive impulse of the blast wave and the severity of the lung injury were closely related to the mortality rate; the frequency of the respiratory and the blood oxygen and the carbon dioxide pressure can roughly exhibit how severe the lungs were injured. Conclusions The severe hypoxia induced by lung injury is the main cause of the early death; correlation between the severity of the injury and the positive impulse of blast wave is very obvious.