Objective To investigate the effects of rhGH on rat model of postoperative fatigue syndrome (POFS) and to study its mechanism. Methods A total of 36 SD rats were randomly divided into 3 groups. The model group and rhGH group were estalished into the model of POFS by partial resection of the liver, and administrated with the same volume of physiological saline and rhGH, and control group was without any treatment. The behavioral changes and the disorder of nutrition intake after operation, stress reaction (pathological changes of mucous membrane in small intestine) and the liver albumin expression were observed. Results The rhGH could improve behavioral changes of rat model and increase the serum levels of the iron, total protein, albumin and globulin as the index of nutrition, and restore the injury of the mucous membrane resulted from the stress reaction and increase the expression of the liver albumin. Conclusion rhGH can shorten the time of POFS and mitigate POFS of rat model.

Objective To establish and assess a model of abdominal postoperative fatigue syndrome (POFS) in rats. Methods After 70% hepatectomy was performed, the following observations of the animals were made:general condition, rat tail suspension test,weight carrying swim fatigue test,serum levels of albumin,ferrition,and iron,pathologic assessment of injury of small intestinal mucosa and hepatic albumin gene expression .Results After 70% hepatectomy of the rats,their general candition was poor,the level of physical tolerance decreased,they showed a certain amount of depression,and marked changes were found in nutritional index,stress injury of small intestinal mucosa and hepatic albumin gene expression.Conclusions A 70% hepatectomy rat model has the basic characteristics of clinical abdominal POFS, and can be used as an experimental animal model for the study of abdominal POFS.

Periphral lymphocytes suspended in RPMI-1640 were incubated in plates of 96 wells for 96 hours with 50 ?g/ml of PHA, various concentrations of LDL-ch and0.6 ?M of mevinolin (experimental) or equal volume of its solvent-dimethyl sulfoxide (control). 6 hours before harvesting cells, 0.5?Ci of ~3H-TdR was added to each well, then cells were harvested and their cpm counted. The LDL receptor activity was expressed as mevinolin-mediated lymphocyte proliferation inhibition rate which can be calculated by the following equation:Percent Inhibition Rate=(1-cpm exp./cpm con.)100%.It was found that the lymphocyte proliferation inhibition rate at 5?g/ml of LDL-ch was less than 20% for normal and non-FH hyperlipidemic subjects, 25-55% for FH heterozygotes and over 60% for FH homozygotes. Thus, FH patients were distinguished from normal subjects and non-FH hyperlipidemic patients.

BACKGROUND: There exists a close relationship between the expression of scavenger receptor(SR) and CD14 and the endotoxic lung injury. To further detect the expression of these two receptors and their relationship is helpful to disclose the receptor mechanism of lung inflammatory disequilibrium so as to provide a possible theoretical foundation for sepsis therapy.OBJECTIVE: To investigate the expression of CD14 and SR in murine alveolar macrophages and their effect after trauma and traumatic endotoxin challenge.DESIGN: Randomized and controlled experimental study.SETTING: Fourth Laboratory(a state key laboratory), Trauma, Burns and Combined Injury, the Research Institute of Field Surgery, Daping Hospital,the Third Military Medical University of Chinese PLA.MATERIALS: The experiment was completed in the Research Institute of Field Surgery of Daping Hospital of the Third Military Medical University of Chinese PLA from August 2001 to November 2001. Totally 63 Kunming mice with murine alveolar macrophages were used in the experiment.METHODS: The experimental animals were randomly divided into three groups including control group, trauma group and traumatic endotoxin challenge group. The model mice with both femur fracture and endotoxin challenge were used. Endotoxin at a dose of 5 mg/kg was injected one hour after fracture via tail vein and the dynamic expression of CD14 and SR in murine alveolar macrophages (AM) was detected.RESULTS: Six hours after trauma, the expression of CD14 and that of SR in AM showed the tendency of up-regulation and down-regulation respectively. Furthermore, the post-traumatic endotoxin challenge induced CD14up-regulation and SR down-regulation drastically. Correlation analysis indicated that CD14 and SR showed a significantly negative relationship.CONCLUSION: The study suggested that the dual modulation of CD14 and SR in AM is possibly related with the change of "self-control" to "out of control" of inflammatory reactions in the lungs after endotoxic challenge. The dual regulation of the effective antagonists CD14 and SR are helpful in further understanding lung injury and its repairing mechanism.

AIM:To investigate the effect of tanshinone IIA on the apoptosis of human umbilical vein endothe -lial cells (HUVECs) after high glucose treatment.METHODS:The cell viability was determined by MTT assay.The cell apoptotic rate was examined by flow cytometry with Annexin V-FITC/PI double staining .The expression of Bcl-2 and Bax, and the release of mitochondrial cytochrome C ( Cyt C) were analyzed by Western blotting .RESULTS: Tanshinone IIA significantly inhibited high glucose-induced decrease in cell viability and increased the cell apoptosis .Additionally, after tanshinone IIA treatment , Bax expression and the release of mitochondrial Cyt C were significantly inhibited , while Bcl-2 expression was increased .CONCLUSION: Tanshinone IIA prevents high glucose-induced endothelial cell apoptosis via mitochondria-dependent pathway .

In this paper, we studied the kinetics of nitochondrial lactate dehydrogenase (LDH) and solubilized mitochondrial LDH in the rat liver. The apparent Km values of mitochondrial LDH and solubilized mitochondrial LDH for substrate pyruvate were 50.0 ?mol/L and 33.8 ?mol/L, and those for NADH were 35.3?mol/L and 21.4 ?mol/L, respectively. The apparent Km values of mitochondrial LDH were greater than those of solubilized nitochondrial LDH. The mitochondrial LDH in the rat liver was mainly LDH-5, which could be solubilized by 0.15 mol/L NaQ solution.

In this paper, the effect of asolectin liposome on the activity of rabbit skeletal muscle lactate dehydrogenase (LDH) is reported. The results suggested that asolectin liposome could inhibit LDH activity, KC1 could restore the enzyme activity, and NAD+ and NADH could protect the enzyme from being inhibited by asolectin liposome.

Objective To establish a new non radioimmunoassay for detecting insulin. Methods We put together the techniques of chemiluminescent analysis, flow injection analysis and immunoassay and established a method,flow injection chemiluminescent immunoassay, for insulin determination using sandwhich procedure with the horseradish peroxdase (HRP) as label of antibody and using the detection system of synergistic enhanced chemiluminescence of para phenylpenol and sodium tetraphenylborate with HRP catalysed oxidation of luminol. Results Satisfactory results were obtained in the dynamic range 0.15～60.00 ?U with a relative coefficient of 0 997 2 ( P

Objective To compare the levels of serum CEA, NSE and CYFRA21-1 of patients with lung cancer and those with benign lung diseases, and to evaluate the clinical differential diagnostic value of combined detection of serum CEA, NSE and CYFRA21-1 in patients with lung cancer. Methods CEA, NSE and CYFRA21-1 were determined with enzyme-linked immunosorbent assay in 122 patients with lung cancer and 37 patients with benign lung diseases. The data was analyzed by t test. Results The serum levels of CEA(12.95?33.04ng/ml), CYFRA21-1(4.87?0.638ng/ml) in patients with lung cancer were significantly higher than that in those with benign lung diseases (1.92?0.84ng/ml and 4.87?0.638ng/ml, respectively)(P0.05). The levels of serum CEA, NSE and CYFRA21-1 were related to types of pathology. The highest levels of CEA, NSE and CYFRA21-1 appeared in adenocarcinoma, small cell carcinoma and squamous cell carcinoma, respectively. The level of serum of NSE (26.05?20.69ng/ml) in patients with small cell carcinoma was significantly higher than that of the patients with benign lung disease, adenocarcinoma (11.26?6.97ng/ml) and squamous cell carcinoma (12.71?7.64ng/ml), respectively (P0.05). Conclusion Combined determination of serum levels of CEA, NSE and CYFRA21-1 is helpful in differential diagnosis of lung cancers.

Objective To study the relationship of gastric carcinoma with serum trace elements and helicobacter pylori(Hp).Methods The contents of 6 serum trace elements and helicobacter pylori were determined in gastric carcinoma,chronic gastritis patients and normal control.Results The serum levels of Mg,Ca,Zn and Cu in the patients with gastric carcinoma were significantly lower than those in normal control Mg (0.71?0.15)mmol/L vs (0.97?0.26)mmol/L,Ca (1.68?0.28)mmol/L vs (2.03?0.31)mmol/L,Cu (12.27?5.91)mmol/L vs (16.57?3.55)mmol/L,Zn (16.18?4.77)mmol/L vs (21.78?7.37)mmol/L.The infection rates of Hp in the patients with gastric carcinoma and normal control were 71.4% vs 27.3%(?2=6.201,P=0.013),there was significant diversity.Conclusion Obvious changes of serum Mg,Ca,Cu,Zn and infection Hp may be related to carcinogenesis of gastric carcinoma.