Objective To investigate the effects of hypertonic saline in the treatment of children with critical hand, foot and mouth disease (HFMD).Methods The 250 pediatric patients with HFMD hospitalized from January 2011 to Januar-y 2013 in our hospital were randomized into the control group treated routinely and the experimental group treated with hyper-tonic saline on the basis of general conventional treatment .The therapeutic effect and sodium ion concentration in the children of two groups were observed and compared.Results The treatment effect of the experimental group was obviously better than that of the control group (p<0.05).Sodium ion concentration and incidence of adverse reactions in two groups before treat-ment showed no significant differences (p>0.05), while the sodium ion concentration of the experimental group was much higher than that of the control group on the 4th and 6th treatment day (p<0.05).Conclusion Hypertonic saline treatment can significantly enhance the therapeutic effect, increase the sodium ion concentration and improve the prognosis in children with critical HFMD.Therefore, hypertonic saline treatment for patients with clinical HFMD has a very important clinical sig-nificance and it is worth popularization and application.

Objective To understand the effect of insulin-like growth factorⅠ (IGFⅠ) on the adaptation of the small intestine in growth hormone (GH)-treated rats. Method 20 SD rats were randomized into GH group and STD group. Parenterally fed, short bowel rat models were established. The morphological changes of the intestinal mucosal epithelia were examined, serum GH, IGFⅠ concentrations were determined by RIA method. The local intestinal IGFⅠ mRNA was evaluated by Northern blot method. Result At the end of the study, mucosal thickness, villous height, crypt depth in GH group (471?16, 299?17, 161?20 ?m respectively) increased significantly compared to that in STD group (374?13, 212?19, 96?9 ?m respectively, all P

Objective To determine species, counts, and seasonal variation of airborne pollen in the center of Nanchang. Methods Pollen counts were done with Durham gravity method on the top of the building in the campus of Jiangxi Medical College, Nanchang, April 1, 2000-March 31,2002. Results There were airborne pollens in all seasons of the year in atmosphere of Nanchang, which included about 47 species. The highest airborne presence (percent of total yearly pollen counts) was for Ambrosia(35.73%); followed by Pinaceae (15.94%), Artemisia (11.94%), Gramineae (5.88%), Cupressaceae (5.64%), Cunningha-mia R.Br.(5.12%), Chenopodium-Amaranthus (5.03%) , and Humulus(2.02%). The dispelling period showed a double curve: the first peak occured in Mar. and Apr. (22.56% and 11.98% respectively). The predominant pollens were tree pollen (Pinaceae, Cupressaceae, Cunninghamia R.Br., Moraceae and Salix). The second peak occured in Aug., Sep., and Oct. (9.61%, 23.77%, 19.57% respectively). The predominant pollens were grass pollen (Ambrosia, Artemisia, Gramineae, Chenopodium-Amaranthus and Humulus). Gramineae had been scattering all seasons, but it's counts were not high in every month. Conclusion There are airborne pollens dispelling in atmosphere of Nanchang in the whole year, whose dispelling period shows a double curve (the first peak occurs in Mar. and Apr. And second peak occurs in Aug. Sep. and Oct.) and the predominant pollens are Ambrosia, Pinaceae, Artemisia, Gramineae, Cupressaceae, Cunninghamia R.Br., Chenopodium-Amaranthus and Humulus. This study provides useful scientific information for production of specific allergen and for clinical prevention, specific diagnosis, specific therapy of allergic deseases.

Aim To investigate the protective effect of propofol on ischemia-reperfusion(I/R)injury in isolated rat hearts and clarify the possible molecular mechanism from oxidative stress and the apoptosis initiated by mitochondria pathway. Methods The langendorff model of ischemia-reperfusion was used.Forty isolated perfused rat hearts were divided into control,I/R, propofol 15,30,60 ?mol?L-1 groups. Hearts were suffered globally ischemic for 25 min and 30 min with reperfusion. The cardiac function indexs such as the left ventricular developed pressure(LVDP), the left ventricular end diastolic pressure(LVDEP), heart rate (HR), coronary arterial flow (CF) were recorded at the time of equilibrate, before ischemia, the end of reperfusion respectively. The lactate dehydrogenase (LDH), creatine kinase (CK) activities in the flow were measured. The swelling and activity of mitochondria, the activity of Manganese Superoxide Dismutase (Mn-SOD) and content of malondialdehyde (MDA) in myocardium mitochondria were also determined. The incidence of cardiomyocyte apoptosis was evaluated by the TdT-mediated dUTP nick end labeling (TUNEL) staining and the expression of Bcl-2 and Bax were evaluated by Flow Cytometry(FCM). The expression of Caspase-3,8,9 was detected by immunohistochemistry.Results Compared with I/R group, administration of propofol at the concentration of 30 and 60 ?mol?L-1 markedly ameliorated the cardiac function in CF,LVDP and LVDEP(P

Objective To explore the effect of rehabilitation training start time after mastectomy on the recovery of the affected limb function. Methods 90 patients who received modified radical mastectomy were divided into groups A, B and C randomly. All the patients accepted the unified rehabilitation program, but started 1, 3 and 10 d after the operation respectively. They were evaluated with the Japanese Orthopaedics Association (JOA) assessment for affected shoulder 1, 3 and 6 months after operation. Results The score of JOA was the least in the group C (P<0.01) 1 month after operation, and was the most in the group A 3 months after operation (P<0.01). At the end of the 6th month, there was no difference among these groups (P>0.05). Conclusion The rehabilitation training can be started as early as possible.

BACKGROUND: Clerodendron bungei steud(CBS) is a tree from genera of Verbena L. In the present pharmacological studies, CBS has showed anti-inflammatory, antineoplasic, nonspecific immunity-enhancing effects. Myoelectric effect of stimulating uterus round ligament is related with agitating adrenergic α receptor. The experiments about analgesic effect are few.OBJECTIVE: To observe the antinociceptive effect of ethanol extract of CBS in mice through hot plate and writhing tests.DESIGN: Randomized controlled experiment with animals as subjects.SETTING: Departmcent of Pharmmacology, Gannan Medical College.MATERIALS: The experiment was performed in Department of Pharmacology, Gannan Medical College from March to June 2004. A total of 120Kunming white mice, weighing(20 ± 2) g were provided by the Experiment Animal Center of Gannan Medical College.INTERVENTIONS: In the writhing test, 50 mice were randomly divided into normal saline, aminopyrine group(0. 1 g/kg), morphine group(0.01 g/kg)and extract of CBS groups(20 g/kg, 40 g/kg) . There were 10 mice in each group. Forty minutes after intraperitoneal injection. 6 mL/L acetic acid (10 mL/kg) was injected intraperitoneally, 5 minutes later, the number of writhing body and inhibitory rate of writhing body were observed for 10minutes. In hot plate test, 40 mice were randomly divided into normal saline group, morphine group(0.01 g/kg) and extract of CBS groups(20 g/kg,40 g/kg). There were 10 mice in each group. After intraperitoneal injection,the mice were put on the hot plate, and the temperature was(55 ±0.5) C. The pain threshold was recorded 15, 30 and 60 minutes after administration. And 30 white mice were randomly divided into naloxone 0. 04 g/kg + morphine 0.01 g/kg group, aloxone 0.04 g/kg + extract of CBS 40 g/kg group,naloxone 0.04 g/kg + normal saline group for hot plate test antagonized by naloxone(40 mg/kg) . The mice were injected intraperitoneally. The duration of pain reaction were recorded 15, 30, 60 and 90 minutes after administration.MAIN OUTCOME MEASURES: ① The number of writhing body and inhibitory rate of writhing body; ② The duration of pain reaction; ③ The pain threshold in the hot-plate test after antagonized by naloxone were calculated.RESULTS: All the 120 white mice entered the result analysis. ① The number of writhing body and inhibitory rate of writhing body: The number of writhing body was 2.4 ± 2.5 and 0. 6 ± 1.7 in the 20 and 40 g/kg extract of CBS injection group respectively and the inhibitory rate of the pain reaction caused by 6 mL/L acetic acid was 93.3% and 98.3% respectively. ② The pain threshold at 15, 30 and 60 minutes after administration in the hot plate test: the pain threshold was[(121.2±98.7) s, (191.2±78.6) s,(133. 1 ± 91.1 ) s] at 15, 30 and 60 minutes after injection of 20 g/kg extract of clerodendron bungei steud; it was[(233.9 ±70.4) s, (219.6 ±78.2) s,(218.3 ±92.6) s at 15, 30 and 60 minutes after 40 g/kg extract of CBS being given. The pain threshold in the extract of CBS groups was all higher than that in the control group respectively[ (13.7 ± 15.2) s, (9.7 ± 12.5) s,(22. 1 ± 15.6) s] ( P ＜ 0.01 -0. 001) . ③ In naloxone antagonized morphine test, the pain threshold at 15, 30 and 60 minutes after administration in morphine 0.01 g/kg+naloxone 0.04 g/kg group[(1.7±5.2) s (6.4 ± 8.6) s, (21.8 ± 34.0) s]was obviously smaller than that in the extract of CBS 40 g/kg +naloxone 0. 04 g/kg group[(124.9 ±79.4) s,(139.3±72.9) s, (137.9±60.8) s](P ＜0.001).CONCLUSION: Ethanol extract of CBS has strong analgesic effect and this is not manifested through the activation of opioid receptors.

AIM To investigate the protective effect of propofol on ischemia-reperfusion (I-R) injury in isolated rat hearts and clarify the possible molecular mechanism from oxidative stress and the apoptosis initiated by mitochondria pathway. METHODS The isolated Langendorff-perfused rat hearts were rendered globally ischemia for 25-min followed by 30-min reperfusion to establish I-R injury model. The cardiac function parameters were recorded. The swelling, integrity of electron transport chain (ETC) and content of malondialdehyde (MDA) in myocardium mitochondria were determined. The percentage of apoptotic cardiomyocytes and the expressions of Bcl-2 and Bax proteins were evaluated by flow cytometry. The expressions of caspases-8, -9 and -3 proteins in myocytes were detected by immunohistochemistry. RESULTS Compared with I-R group, perfusing with 30 and 60 μmol·L-1 propofol from 10 min before ischemia to whole reperfusion period resulted in improvement in cardiac function. The swelling and ETC lesion of mitochondria alleviated, and MDA content decreased. The percentage of apoptotic cardiomyocytes was markedly lower than that of I-R group. The expression of Bcl-2 protein was higher and the expression of Bax was lower than that of I-R group. The expressions of caspase-3 and caspase-9 proteins were obviously lower than those in I-R group. CONCLUSION Propofol confers significant protection against the I-R injury in the isolated rat hearts. Diminishing oxidative stress, protecting mitochondria from peroxidative injury, thus interfering with the mitochondria-dependent apoptotic pathway may be one of the major mechanisms of its cardioprotection.

BACKGROUND: It is commonly believed that hyperbaric oxygen (HBO)plays a neuroprotective role in the prevention and treatment of cerebral infarction (CI), however hyperbaric oxygen therapy (HBOT)-induced CI was also reported.OBJECTIVE: To investigate the risk factors of HBOT-induced CI.DESIGN: Case analysis with patients as subjects.SETTING: At the Department of Hyperbaric Oxygen of Guangzhou General Hospital of Guangzhou Military Command Area of Chinese PLA.PARTICIPANTS: Totally 192 inpatients receiving HBOT were selected from the Department of Hyperbaric Oxygen of Guangzhou General Hospital Guangzhou Military Command Area of Chinese PLA between December 1996 and March 1998, including 127 males and 65 females with age ranging from 9 to 78 years old. Totally 6 patients, 3 males and 3 females, suffered from CI during HBOT, with the age from 51 to 76 yeas.METHODS: The background factors of 192 patients who HBOT METHODS and 6 cases with induced CI were analyzed.MAIN OUTCOME MEASURES: Symptoms, signs and skull CT or MRI.RESULTS: Data of 192 patients were dealt with intentionally and all were statistically analyzed without miss. ① The incidence of CI during HBOT was 0.3% (6/192). ② The risk factors of HBOT-induced CI included: age of ≥ 60 years, hypertension, hyperlipidemia, primary CI or cerebral hemorrhage, blood hyperviscosity and diabetes, and the incidence would greatly increased in those who has 4 or more than 4 risk factors, but seldom with less than 4 risk factors.CONCLUSION: The cluster level of risk factors was closely related with the incidence of HBOT-induced CI.

AIM:To investigate whether hydrogen sulfide ( H2 S) protects the hearts against inflammatory re-sponses induced by acute myocardial ischemia in isolated rat hearts .METHODS: Rat acute myocardial ischemia injury was induced by ligation of the left anterior descending coronary artery for 4 h, and the normal perfusate was replaced with NaHS (5 μmol/L, 10μmol/L and 20 μmol/L) perfusate accordingly in NaHS groups 2 h after ischemia.The changes of cardiac function in the myocardial ischemic injury rats were observed .The mRNA expression of TNF-α, IL-1β, IL-6, IL-10 and ICAM-1 was detected by real-time PCR.The protein level of nuclear factor-κB ( NF-κB) in the myocardial tissues was detected by Western blotting .RESULTS:The cardiac function in ischemia group was lower than that in sham group (P<0.01).Compared with ischemia group, perfusion of NaHS resulted in the improvement of the cardiac function (P<0.05 or P<0.01).Compared with sham group, the mRNA expression of TNF-α, IL-1β, IL-6 and ICAM-1 in the cardiac tissues was significantly increased , and the mRNA expression of IL-10 in the cardiac tissues was significantly decreased in ischemia group (P<0.01).Compared with ischemia group , the perfusion of NaHS significantly decreased the mRNA ex-pression of TNF-α, IL-1β, IL-6 and ICAM-1 ( P<0.05 or P<0.01 ) .The perfusion of NaHS at concentrations of 10μmol/L and 20μmol/L significantly increased the mRNA expression of IL-10 (P<0.01).The protein level of NF-κB in ischemia group was markedly higher than that in sham group (P<0.01).Compared with ischemia group, the perfusion of NaHS at concentrations of 10 μmol/L and 20 μmol/L significantly decreased the expression of NF-κB ( P<0.05 or P<0.01).CONCLUSION:H2S protects the hearts against acute ischemia injury through inhibition of NF-κB activation and subsequent down-regulation of NF-κB-dependent inflammatory gene expression .

Objective To explore the clinical value of plasma D -D and blood glucose change in treating patients with bone trauma.Methods Selected 200 patients with bone trauma as the observation group,60 cases of healthy persons as the control group.The plasma D -D and blood glucose of the two groups were detected,and the results were analyzed.Results The plasma D -D and blood glucose of the observation group were more than those of the control group,and it was gradually reduced from first day to third day and fifth day.The plasma D -D of the observation group was (174.7 ±40.2)μg/L,blood glucose was (5.2 ±0.4)mmol/L.The plasma D -D of first day, third day and fifth day of the observation group were (1 452.6 ±303.4)μg/L,(982.2 ±215.2)μg/L,(705.6 ± 152.9)μg/L respectively.The blood glucose of first day,third day and fifth day of the observation group were (14.2 ±2.3)mmol/L,(10.3 ±1.2)mmol/L,(7.6 ±1.9)mmol/L,respectively.The difference was statistically sig-nificant(F =3.49,P <0.05).In the observation group,the more severe injury in patients,the higher plasma D -D and blood glucose,and it was gradually reduced from first day to third day and fifth day,the difference was statistically significant(F =4.83,P <0.05).Conclusion In judging the condition of bone trauma,it is important to measure plasma D -D and blood glucose levels,and this method should be popularized in clinical use.