Neurogenesis is sustained throughout adulthood in the mammalian brain due to the proliferation and differentiation of adult neural progenitor cells found in the subventricular zone of the lateral ventricles and subgranular zone of the dentate gyrus. This review covers recent findings that elucidate different aspects of regulation of neurogenesis, including proliferation, migration and differentiation into mature neurons and functional integration into the existing neural circuits. Furthermore, this review also discusses the effects of pathological conditions on adult neurogenesis in both rodent models and human patients as well as some of the potential problems or limitations in neurogenesis research, which may shed some light on developing novel research strategies for replacement treatment of neurological disorders.

Objective: To investigate the quality control for purpose of radiologic protection in beside Photography. Methods: From a clinical application, based on the thress principle of radiological protection, combined with years of clinical experience, the need for comprehensive study bedside photography and protection security. Depending on the situation ,put forward corresponding countermeasures. Results: The different patient, especially in children photography and treatment of critically ill patients, with a reasonable operation mode, the method can reduce the radiation. Conclusion:The widespread popularity of medical radiological protection education, strengthen the quality control of bedside photography on bedside X-ray photography protection is very useful.

Objective: To explore the feature of theory of mind(ToM) in schizophrenic patients and the influencing factors. Methods: Subjects were 108 patients with schizophrenia, 21 with affective disorders and 21 normal subjects. Picture-story tasks, PANSS and WAIS-R were used to assess the patients' ToM, symptoms and intelligence. Results:It was found that the scores on first-order theory of mind tasks of schizophrenic patients with IQ below 70 were lower than normal subjects'(D = -56.58,P

The basic nursing quality is an important dial of the general administration level and the basic personnel quality of a hospital.Many causes have led to the down trend of the basic nursing quali- ty.After a specific analysis of the causes of such a trend,important measures and solutions have been put forth,The first is to strengthen the moral education of the profession and upgrade the quality of the nursing staff.The second is to adopt the "group responsibility" administration and gradually carry out a systimatic integrated nursing so that a transformation of nursing models is put to practice,The third is to broaden the outlet of human resources so that personnel are rationally employed and the rate of job— taking raised.The last one is to heighten the initiative of the nursing staff through the rational opera- tion of the "incentive"mechanism in accordance with the law of values.

Objective To determine species, counts, and seasonal variation of airborne pollen in the center of Nanchang. Methods Pollen counts were done with Durham gravity method on the top of the building in the campus of Jiangxi Medical College, Nanchang, April 1, 2000-March 31,2002. Results There were airborne pollens in all seasons of the year in atmosphere of Nanchang, which included about 47 species. The highest airborne presence (percent of total yearly pollen counts) was for Ambrosia(35.73%); followed by Pinaceae (15.94%), Artemisia (11.94%), Gramineae (5.88%), Cupressaceae (5.64%), Cunningha-mia R.Br.(5.12%), Chenopodium-Amaranthus (5.03%) , and Humulus(2.02%). The dispelling period showed a double curve: the first peak occured in Mar. and Apr. (22.56% and 11.98% respectively). The predominant pollens were tree pollen (Pinaceae, Cupressaceae, Cunninghamia R.Br., Moraceae and Salix). The second peak occured in Aug., Sep., and Oct. (9.61%, 23.77%, 19.57% respectively). The predominant pollens were grass pollen (Ambrosia, Artemisia, Gramineae, Chenopodium-Amaranthus and Humulus). Gramineae had been scattering all seasons, but it's counts were not high in every month. Conclusion There are airborne pollens dispelling in atmosphere of Nanchang in the whole year, whose dispelling period shows a double curve (the first peak occurs in Mar. and Apr. And second peak occurs in Aug. Sep. and Oct.) and the predominant pollens are Ambrosia, Pinaceae, Artemisia, Gramineae, Cupressaceae, Cunninghamia R.Br., Chenopodium-Amaranthus and Humulus. This study provides useful scientific information for production of specific allergen and for clinical prevention, specific diagnosis, specific therapy of allergic deseases.

To examine the occurrence of neuron activation,neurodegeneraion and cell death,and the correlation among them in the hippocampus after status epilepticus.Methods CFV,Fluoro Jade B and c-Fos staining were done at multiple time points after pilocarpine induced status epilepticus.Results In the stratum granulosum of dentate gyrus,c-Fos positive neurons increased significantly at 1 h,2 h and 1 d after status epilepticus (P ＜0.01 or 0.05).However,almost no Fluoro Jade B staining cell was found in the stratum granulosum in the experiment and control groups,and no obvious difference was shown on the numbers of CFV staining cells in this area among all groups.In the hilus of dentate gyrus of different groups,there were no c-Fos positive neurons in all groups.In the hilus,the number of Fluoro Jade B staining cells significantly increased at 2 h and 1 d after the status epilepticus (P ＜0.01 ),and the number of CFV staining neurons dramatically decreased 1 d after the status epilepticus ( P ＜ 0.01 ) compared with the control.In the stratum pyramidale of CA1,the numbers of c-Fos positive neurons at 30 mim,1 h,2 h and 1 d,and Fluoro Jade B staining cells at 2 h and 1 d after the status epilepticus significantly increased (P ＜ 0.01 or 0.05 ),while no obvious difference in the number of CFV staining cells in the stratum pyramidale of CA1 among different groups was shown.Conclusion There is no direct correlation among cell activation,neuron degeneration and cell death in the hippocampus of mice after pilocarpine induced status epilepticus.

Objective To assess the relationship between polymorphism of XRCC1 Arg194Trp Arg399Gln and ESCC TNM stage and regional lymph node metastasis. Methods PCR and PCR-RFLP were used to detect polymorphism of XRCC1 Arg194Trp and Arg399Gin. The results were analyzed and compared to TNM staging and regional lymph node metastasis. Results The XRCC1 194Arg/Trp and 399Arg/Arg wildtype in ESCC with metastasis is 32.3%, which is higher than 194Arg/Arg (20.0%, P=0.01). There was no remarkable difference between IIb-IV stage ESCC with lymph node metastasis and 0-IIa stage without metastasis. Conclusion The ESCC patients with 194 variability and 399 wildtype had high metastasis rate, XRCC1 Arg194Trp and Arg399Gln had relationship with ESCC TNM stage and lymph node metastasis. Both of them affected to prognosis of ESCC.

Objective To study the primary volume of bone cement and the method of perfusion and the clinical effect of percutaneous vertebroplasty for bone metastatic tumors. Methods Percutaneous vertebroplasty was performed in 218 vertebral bodies of total 150 patients,including 92 males and 58 females with age of 26-85,averaging 56.8 y. 218 vertebral bodies included 73 dorsal vertebra and 138 lumber vertebra and 7 sacral vertebra. Operations were taken under the DSA monitoring. The approach route was selected according to the morphology of the vertebrae,damage extent and puncture site. Results Operation was successful in all 218 vertebrae of total 150 patients with a ratio of 100%. The primary volume of bone cement needed to fill dorsal vertebra was 2-5 ml,average 3.5 ml; that for lumber vertebra was 3-8 ml,average 5.5 ml; and that for sacral vertebra was 4-7,average 5.5 ml. 134 patients showed total pain relief (89.3%); 15 patients had partial remission (10%) and 1 failure (0.7%). The cement extravasated to intervertebral disk in 12 patients,to scleromeninx capsula externa in 10,to para-vertebra plexus venosus in 3 and to paravertebra soft tissue in 4. The incidence of leakage of bone cement was 13.3%,but no complications occurred during and after the operation. Concolusion Percutaneous vertebroplasty provides obvious pain relief effect for vertebral metastasis,worthy to be recommended widely in clinical application.

Objective To investigate the protective effect of osthole on focal cerebral ischemia-reperfusion injury in rats.Methods Focal cerebral ischemia-reperfusion model in rat was made by transient occlusion of the middle cerebral artery for 2 h and followed by reperfusion for 24 h.Osthole 5 and 10 mg/kg were iv injected through sublingual vein at 1 h after the onset of ischemia,respectively.After 24 h of reperfusion,the influence of osthole on neurological behaviour deficit score,brain edema,and infarct size were evaluated.The activity of Na+,K+-ATPase,Ca2+-ATPase,and myeloperoxidase(MPO)in the ischemic hemisphere cortex of the middle cerebral artery area was assayed by spectrophotometry.The level of IL-8 was detected with radioimmunoassay.Results Osthole significantly reduced the neurological behaviour deficit score,brain edema,and infarct size,enhanced the activity of Na+,K+-ATPase and Ca2+-ATPase,inhibited the activity of MPO,and decreased the level of IL-8 in the brain tissue.ConclusionThe results suggest that osthole could attenuate the brain damage following focal cerebral ischemia-reperfusion in rats and its mechanism may be partly related to the inhibition of inflammation and brain edema induced by ischemia-reperfusion.

0.05).The stimulative index(SI)tested by MTT in MLR at stimulator-to-reactor cell ratio 1∶1,1∶10,1∶20,1∶40 and 1∶80 was 2.7,4.1,3.1,2.5 and 1.6,respectively.The proliferation of T cells stimulated by DCs could be detected at all stimulator-to-reactor ratio.Conclusion DCs could be successfully induced from peripheral blood monocyte in the patients with colorectal cancer by AIM-V serum-free media or by traditional FBS-contained media.AIM-V is more suitable for clinical immunotherapy because it can avoid the risk of allergic reaction for clinical purpose.AIM-V might take the place of serum-contained media to culture DCs.According to the results of MLR,DCs could stimulate allogenetic T cells proliferation at all stimulator-to-effector ratio,furthmore the optimal ratio is 1∶10 .