Human embryonic stem(hES) cells can self-renew and have the ability to differentiate into any type of cells of the body. These characteristics make hES cells a good candidate for cell-based therapies. Current techniques for derivating and culturing embryonic stem cells are very mature. However, concerns arise that pathogen contamination may make these cells unsuitable for therapeutic purposes. An optimal growth environment is greatly needed. Through various in vitro differentiation methods, human embryonic stem cells can be induced into many specialized cell types. However the mechanism of committed differentiation is still unknown.

Objective To investigate the existence and variance of quinolone-resistance genes in a group of pan-drug resistant of Acinetobacter baumannii ( A.baumannii ).Methods Twenty strains of pandrug resistant A.baumannii were isolated from patients registered in Affiliated Hospital of Nantong University from January 2011 to April 2011.Drug target genes to quinolone (gyrA,parC) and quinolone-resistance genes mediated by mobile genetic elements [ qnrA,qnrB,qnrS,aac(6')-Ⅰ b-cr,qepA] were analyzed by PCR and verified by DNA sequencing.Results In all 20 strains of A.baumannii,the sense mutation was found in the quinolone resistance-determining region of the gyrA gene in the form of TCA to TTA at codon 83 (Ser-83-Leu).Moreover,in the quinolone resistance-determining region of the parC gene sense mutation was found in the form of TCG to TTG at codon 80 (Ser-80-Leu) and 3 synonymous mutations were CCC to CCT at codon 40,GTA to GT]T at codon 41 and CGT to CGC at codon 44.And parC gene was a new mutation.However,mutations were not found in quinolone-resistance genes mediated by mobile genetic elements [ qnrA,qnrB,qnrS,aac( 6 ' )-Ⅰ b-cr,qepA ].Conclusion Quinolone-resistance-determining region play a key role in resistance to quinolones in this group of A.baumannii.To our knowledge,this is first report about the emergence of the new mutation of parC gene in China.

Objective: To explore the effect of comprehensive yoga intervention program on cardiopulmonary function and physical fitness among female college students. Methods: Twohundred and forty female college students were invited to participate in the program and were randomly divided into intervention (n=120) and control group (n=120) by random number table method. The intervention group received regular yoga exercise, while the control group had no exercise intervention. Body shape, cardiopulmonary function, body flexibility, muscle strength and mental health of the two groups were compared before and after intervention. Results: The cardiopulmonary function indices except PP of the intervention group improved significantly after intervention. There were no significant changes in cardiopulmonary function before and after the intervention for control group. The HR values of the observation group were significantly lower, and the values of SV, CO, VC, MVV and FEV1/FEV were significantly higher compared with those of the control group(P<0.05). Body mass index(BMI), waisttohip ratio and body fat percentage of observation group were significantly lower than that of control group(P<0.05). The performance on the sitandreach and situp test of observation group were significantly improved after intervention and were significantly higher than the control group(P<0.05). Somatization, sensitivity, depression and anxiety in the observation group decreased significantly after intervention and were lower than that of the control group(P<0.05). Conclusion The comprehensive yoga intervention program can effectively improve body shape, body flexibility and muscle strength, as well as improve cardiopulmonary function. It also plays a key role in improving mental health among female college students.

Objective To evaluate the effect of lung cancer bone metastasis on serum osteocalcin(OC)in Chinese population using me-ta-analysis.Methods Searched the date of PubMed,Web of science,Embase,Cochrane Library,CBM,CNKI,VIP database and Wanfang da-tabase to identify potentially studies which involved the correlation between bone metastasis and serum OC in Chinese patients with lung canc-er.Standardized mean difference(SMD)and 95%CI was used to access the results.Results 8 studies were included.The study showed that the OC in serum would increase significantly when bone metastases happened in Chinese lung cancer patients.SMD(95%)CI was 0.58 [0.15,1.01]and 0.776[0.20,1.34]compared with controls and BM-,respectively.SMD and 95%CI of BM-compared with controls was -0.15[-0.54,0.23],insignificantly.Conclusion Lung cancer bone metastases may raise serum osteocalcin levels in Chinese popu-lation meanwhile this phenomenon will not happen without bone metastasis.

Embryonic stem cell is promising for regenerative medicine. However, its application is hampered by the utilization of eggs in most established methods. Recently, a new pluripotent stem cell establishing method was reported that, mouse and human differentiated cells could be induced reprogrammed into a pluripotent state by expressing exogenetic stem factors such as Oct4, Sox2, et al, through retroviral transduction. This approach avoiding egg use is a great breakthrough not only in stem cell technology but also present theory hypothesis of reprogramming. Here these works were reviewed in this article. Both the mechanism of induced reprogramming and the prospects of induced pluripotent stem cells were discussed.
Animals ; Cell Differentiation ; genetics ; Cells, Cultured ; Cellular Reprogramming ; drug effects ; genetics ; Humans ; Octamer Transcription Factor-3 ; metabolism ; Pluripotent Stem Cells ; cytology ; Retroviridae ; genetics ; SOXB1 Transcription Factors ; metabolism ; Transduction, Genetic

Objective To establish an analytical method combining gas chromatography-tandem mass spectrometry(GC-MS)and liquid chromatography-tandem mass spectrometry(LC-MS)to detect diazepam residue in bait nest materials and fish samples,and improve the pretreatment steps of samples to make the experimental results accurate and the sample processing convenient and fast.Methods Taking diazepam as the research object,samples were extracted with methanol and dichloromethane/n-hexane as solvents according to the type,and the supernatant was taken for detection after centrifugation.Results The diazepam standard sample showed a good linear relationship in the range of 10～10 000 ng/mL.The retention times in methanol and mixed solvent were 13.54 min and 13.83 min,respectively,and the correlation coefficients were 0.998 and 0.999,respectively;The limit of detection(LOD)of using methanol as extraction solvent was 2 ppb,and limit of quantification(LOQ)was 6 ppb.The LOD of using mixed solvents was 5 ppb,and the LOQ was 15 ppb.When the sample is a bait nest material,the GC-MS spectrum was clear and standard,and the peak shape was sharp and prominent;When the sample is biological specimen,the GC-MS spectra are disturbed by matrix,while the LC-MS data is more accurate and faster.Conclusion It is more appropriate to use GC-MS to determine the content of bait nest materialsamples,and it is more accurate to LC-MS to determine the content of fish samples due to the complexity of the organism.

Objective To investigate the effects of acute sleep deprivation on the behavior and synaptic protein expression of rats.Methods Seventy healthy male Wistar rats were randomly divided into seven groups,a Control group and sleep deprivation groups(24,48,72,96,120 and 144 hours).The sleep deprivation rat model was established by the modified multiplatform water environment sleep deprivation method.Spatial learning and memory were assessed by the Morris water maze.Anxiety was assessed by the open field test.The morphology and quantity of hippocampal neurons were observed by Nissl staining.Western blot and Real-time PCR were used to determine the expression of synaptophysin(SYN),post-synaptic density protein-95(PSD-95),and brain-derived neurotrophic factor(BDNF)in rats.Results Compared with the Control group,the numbers of standing and modification were significantly increased by prolongation of the sleep deprivation time(P＜0.05).The escape latency and path length were significantly increased in 120 and 144 h groups(P＜0.05),whereas the number of platform crossings and the percentage of the target quadrant time were significantly decreased(P＜0.01)and negatively correlated to the sleep deprivation time.The expression levels of BDNF,SYN,and PSD-95 were significantly decreased with the prolongation of sleep deprivation time(P＜0.01).Conclusions With the increase in sleep deprivation time,cognitive dysfunction and anxiety gradually deteriorated,which may be related to decreases in the expression of synaptic biomarkers.

Patients with severe trauma require an extremely timely treatment and transfusion plays an irreplaceable role in the emergency treatment of such patients. An increasing number of evidence-based medicinal evidences and clinical practices suggest that patients with severe traumatic bleeding benefit from early transfusion of low-titer group O whole blood or hemostatic resuscitation with red blood cells, plasma and platelet of a balanced ratio. However, the current domestic mode of blood supply cannot fully meet the requirements of timely and effective blood transfusion for emergency treatment of patients with severe trauma in clinical practice. In order to solve the key problems in blood supply and blood transfusion strategies for emergency treatment of severe trauma, Branch of Clinical Transfusion Medicine of Chinese Medical Association, Group for Trauma Emergency Care and Multiple Injuries of Trauma Branch of Chinese Medical Association, Young Scholar Group of Disaster Medicine Branch of Chinese Medical Association organized domestic experts of blood transfusion medicine and trauma treatment to jointly formulate Chinese expert consensus on blood support mode and blood transfusion strategies for emergency treatment of severe trauma patients ( version 2024). Based on the evidence-based medical evidence and Delphi method of expert consultation and voting, 10 recommendations were put forward from two aspects of blood support mode and transfusion strategies, aiming to provide a reference for transfusion resuscitation in the emergency treatment of severe trauma and further improve the success rate of treatment of patients with severe trauma.