1.Derivation, culture and in vitro differentiation of human embryonic stem cells
International Journal of Biomedical Engineering 2006;0(04):-
Human embryonic stem(hES) cells can self-renew and have the ability to differentiate into any type of cells of the body. These characteristics make hES cells a good candidate for cell-based therapies. Current techniques for derivating and culturing embryonic stem cells are very mature. However, concerns arise that pathogen contamination may make these cells unsuitable for therapeutic purposes. An optimal growth environment is greatly needed. Through various in vitro differentiation methods, human embryonic stem cells can be induced into many specialized cell types. However the mechanism of committed differentiation is still unknown.
2.A novel variant of topoisomerase Ⅳ gene in a group of pan-drug resistant Acinetobacter baumannii
Shaopeng CHU ; Guihua WANG ; Rongrong JING ; Jianxin WANG ; Shaoqing JU
Chinese Journal of Microbiology and Immunology 2012;32(2):157-160
Objective To investigate the existence and variance of quinolone-resistance genes in a group of pan-drug resistant of Acinetobacter baumannii ( A.baumannii ).Methods Twenty strains of pandrug resistant A.baumannii were isolated from patients registered in Affiliated Hospital of Nantong University from January 2011 to April 2011.Drug target genes to quinolone (gyrA,parC) and quinolone-resistance genes mediated by mobile genetic elements [ qnrA,qnrB,qnrS,aac(6')-Ⅰ b-cr,qepA] were analyzed by PCR and verified by DNA sequencing.Results In all 20 strains of A.baumannii,the sense mutation was found in the quinolone resistance-determining region of the gyrA gene in the form of TCA to TTA at codon 83 (Ser-83-Leu).Moreover,in the quinolone resistance-determining region of the parC gene sense mutation was found in the form of TCG to TTG at codon 80 (Ser-80-Leu) and 3 synonymous mutations were CCC to CCT at codon 40,GTA to GT]T at codon 41 and CGT to CGC at codon 44.And parC gene was a new mutation.However,mutations were not found in quinolone-resistance genes mediated by mobile genetic elements [ qnrA,qnrB,qnrS,aac( 6 ' )-Ⅰ b-cr,qepA ].Conclusion Quinolone-resistance-determining region play a key role in resistance to quinolones in this group of A.baumannii.To our knowledge,this is first report about the emergence of the new mutation of parC gene in China.
3.Effects of comprehensive yoga intervention program on cardiopulmonary function and physical fitness among female college students
Chinese Journal of School Health 2019;40(1):89-91
Objective:
To explore the effect of comprehensive yoga intervention program on cardiopulmonary function and physical fitness among female college students.
Methods:
Twohundred and forty female college students were invited to participate in the program and were randomly divided into intervention (n=120) and control group (n=120) by random number table method. The intervention group received regular yoga exercise, while the control group had no exercise intervention. Body shape, cardiopulmonary function, body flexibility, muscle strength and mental health of the two groups were compared before and after intervention.
Results:
The cardiopulmonary function indices except PP of the intervention group improved significantly after intervention. There were no significant changes in cardiopulmonary function before and after the intervention for control group. The HR values of the observation group were significantly lower, and the values of SV, CO, VC, MVV and FEV1/FEV were significantly higher compared with those of the control group(P<0.05). Body mass index(BMI), waisttohip ratio and body fat percentage of observation group were significantly lower than that of control group(P<0.05). The performance on the sitandreach and situp test of observation group were significantly improved after intervention and were significantly higher than the control group(P<0.05). Somatization, sensitivity, depression and anxiety in the observation group decreased significantly after intervention and were lower than that of the control group(P<0.05).
Conclusion
The comprehensive yoga intervention program can effectively improve body shape, body flexibility and muscle strength, as well as improve cardiopulmonary function. It also plays a key role in improving mental health among female college students.
4.Effect of lung cancer bone metastasis on serum osteocalcin levels in Chinese population:a meta-analysis of case-control studies
Leyuan ZANG ; Min MA ; Jianxin HU ; Hao QIU ; Bo HUANG ; Tongwei CHU
Journal of Regional Anatomy and Operative Surgery 2016;25(6):442-445
Objective To evaluate the effect of lung cancer bone metastasis on serum osteocalcin(OC)in Chinese population using me-ta-analysis.Methods Searched the date of PubMed,Web of science,Embase,Cochrane Library,CBM,CNKI,VIP database and Wanfang da-tabase to identify potentially studies which involved the correlation between bone metastasis and serum OC in Chinese patients with lung canc-er.Standardized mean difference(SMD)and 95%CI was used to access the results.Results 8 studies were included.The study showed that the OC in serum would increase significantly when bone metastases happened in Chinese lung cancer patients.SMD(95%)CI was 0.58 [0.15,1.01]and 0.776[0.20,1.34]compared with controls and BM-,respectively.SMD and 95%CI of BM-compared with controls was -0.15[-0.54,0.23],insignificantly.Conclusion Lung cancer bone metastases may raise serum osteocalcin levels in Chinese popu-lation meanwhile this phenomenon will not happen without bone metastasis.
5.Induced pluripotent stem cells generated from reprogramming differentiated cells by defined factors.
Xiaoyu XIA ; Jianxin CHU ; Xuejin CHEN
Chinese Journal of Biotechnology 2008;24(7):1121-1127
Embryonic stem cell is promising for regenerative medicine. However, its application is hampered by the utilization of eggs in most established methods. Recently, a new pluripotent stem cell establishing method was reported that, mouse and human differentiated cells could be induced reprogrammed into a pluripotent state by expressing exogenetic stem factors such as Oct4, Sox2, et al, through retroviral transduction. This approach avoiding egg use is a great breakthrough not only in stem cell technology but also present theory hypothesis of reprogramming. Here these works were reviewed in this article. Both the mechanism of induced reprogramming and the prospects of induced pluripotent stem cells were discussed.
Animals
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Cell Differentiation
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genetics
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Cells, Cultured
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Cellular Reprogramming
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drug effects
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genetics
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Humans
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Octamer Transcription Factor-3
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metabolism
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Pluripotent Stem Cells
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cytology
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Retroviridae
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genetics
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SOXB1 Transcription Factors
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metabolism
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Transduction, Genetic