Objective To observe the effects of hand function training with continuous passive motion (CPM) on hand spasm and function of the hemiplegic patients.Methods 60 hemiplegic patients after stroke or brain trauma and with hand spasm were randomly divided into the treatment group and control group with 30 cases in each group. The cases in the treatment group were treated mainly with the method of CPM together with the routine rehabilitation procedures, while the those in the control group were only treated with the routine rehabilitation procedures. The changes of hand spasm and function of two groups were observed.Results After four weeks' treatment, the efficacy of the treatment group was obviously better than that of the control group ( P<0.05).Conclusion The rehabilitation training is an effective method for treatment of hemiplegia, and CPM is an important auxiliary method in the treatment. These two Methods combined together can shorten the course of treatment, and strengthen cure efficacy.

Objective To analyze the expressions of tumor stem cell markers Lgr5 and CD44 in esophageal carcinoma and its clinical pathological significance.Methods A total of 108 cases of patients with esophageal cancer, 35 cases of stage Ⅱ, 40 cases of stage Ⅲ, 4 cases of stage Ⅳ. After tumor specimens were stained, the expressions of Lgr5 and CD44 in esophageal cancer tissue were detected and its relationship with the clinical features were analyzed.Results There were relations between expressions of Lgr5 and CD44 in esophageal cancer tissue and clinical staging, the later the stageing, the higher expression rate of Lgr5 and CD44, but there was no significant differece with Chi-square test. The Spearman correlation analysis showed there was no correlation between Lgr5 or CD44 and esophageal cancer condition. Conclusion CD44 and Lgr5 are highly expressed in esophageal cancer tissue, but the correlation between CD44 and Lgr5 expression and esophageal cancer condition is uncertain.

Objective To investigate the expressing changes of nitric oxide synthase(NOS) in substantia nigra and striatum of the hemiparkinsonism monkey model.Methods Three hemiparkinsonian rhesus monkey models were induced by injecting 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP) unilaterally into the internal carotid artery.The expressing changes of NOS-positive neurons of substantia nigra and striatum in three hemiparkinsonism monkey models and one normal control monkey were observed by using NADPH-diaphorase histochemistry staining method.Results The NOS-positive neurons were increased significantly in the substantia nigra and striatum of MPTP lesioned side of hemiparkinsonian monkey model compared to contralateral side and normal control monkey(all P

Objective To study the relationship between generalized tonic clonic epilepsy of different frequence seizure and erythrocyte ATPase activity.Methods We measured Na +/K + ATPase,Mg 2+ ATPase,Ca 2+ ATPase activity of 68 patients with generalized tonic clonic seizure who were divided into 3 groups by different frequence:Status epilepsy group (SE) for 26 cases,Frequency epilepsy (FE) group for 22 cases,epilepsy (EP) group for 20 cases and the normal controls for 35 cases.Results Erythrocyte Na +/K + ATPase in SE, FE group decreased,as compared with EP group,the normal control group, there was remar kable difference( P

Objective To study the effect of nerve growth factor(NGF)in survival of super-length random flap.Methods The experiment employed the animal model of random flap in Wistar's rat back,applied 66.7 μg NGF locally in trial group's flap at the first day after operation with auto-control.The survival proportion,skin temperature and survival rate of random flap were observed,and the blood flow,the blood flow velocity,the blood flow volume change of random flap were measured by the laser and photic-conduct fiber means in differ time.Results The survival proportion(7.12±1.54)cm2 and survival rate(92±5)%of random flap of trial group was obviously higher(P＜0.05)than those in control group[(5.23±0.19)cm2,(69±5)%]after pedicle division.The blood flow,the blood flow velocity,the blood flow volume and skin temperature in the midge and at the end of random flap in the trial group were obviously higher than those in control group 1 day after operation(P＜0.05),and the blood flow velocity in the pedicle of random flap in the trial group was increased markedly(P＜0.05).The blood flow volume and skin temperature in the pedicle of random flap in the trial group were higher than those in control group 3 days after operation(P＜0.05).The survival of randomflapinthetrial groupwere very significantly increased than those in control group 7 days after operation(P＜0.01).Conclusion NGF can increase flap's blood flow,blood flow velocity and blood flow volume,enhancing survival of the big-proportioned and super-length random flap.

Objective To observe the effect of Huazhuo-Jiedu-Huoxue Tongluo recipe on local inflammation of cerebral ischemia reperfusion injury in mice. Method Fifty Kunming Mice were randomly divided into five groups: model group, high-dose group of TCM, low-dose group of TCM, nimodiping group and sham operation group. Each group had 10 mice. Mice models of the right middle cerebral artery CIRI in the first four groups were established through thread embolism method. Physiological saline was given to the mice in the model group and sham operation group. Nimodipine solution was given to the mice in the Nimodipine group. High and low dose of Huazhuo-Jiedu-Huoxue-Tongluo Chinese medicine decoction were given to the mice in the high dose of TCM group and low dose of TCM group respectively after the models were successfully established. To assess the expression level of IL-6 and TNF-α by radioimmunoassay after the treatment. Results Compared with model group [(219.29±39.28) pg/ml, (1.325 ± 0.236) ng/ml] , the level of IL-6 and TNF-α of high dose of TCM group[(120.69±49.23)pg/ml, (1.086±0.178)ng/ml], low dose of TCM group[(173.97±49.03)pg/ml, (0.937 ± 0.105)ng/ml]and nimodipine group[(170.88± 47.06)pg/ml, (1.092±0.184) ng/ml]decrease (P<0.05). There is no significant difference between the high dose of TCM group and the sham operation group (P>0.05). Conclusion Huazhuo-Jiedu-Huoxue-Tongluo recipe can restrain the expression of inflammatory cytokines and reduce the infiltrating of leukocytes in mice with cerebral ischemia reperfusion injury.

This study evaluated the efficacy and safety of "J"-shaped uterine incision for caesarean section for patients diagnosed with placenta previa. A total of 55 consecutive cases of placenta previa treated in Union Hospital were retrospectively analyzed over a period of two years and 10 months. The subjects were divided into two groups with respect to the uterine incision. Twenty-four pregnant women with placenta previa who were indicated for caesarean section underwent the procedure using a new "J"-shaped uterine incision and 31 pregnant women with placenta previa received caesarean section that used the traditional transverse incision. The two groups were compared in terms of operation time, estimated blood loss, infant expulsion time, exhaust time and postoperative recovery. Meanwhile, comparison was also made in neonatal clinical data between the two groups. Compared with the "J"-shaped incision group, the traditional incision group had a lower Apgar scores (P<0.05). However, there existed no statistically significant differences in the overall time of operation and postoperative period of breaking wind (P>0.05). It is concluded that, with caesarean section for placenta previa patients, the "J"-shaped uterine incision significantly decreases intraoperative blood loss and facilitates the fetal delivery.

Objective To investigate the value of optimizing the thoracic CT scanning dose on reducing radiation dose.Methods 50 patients were undergone CT scanning using CARE Dose 4D technique,the mAs of each slices,CT dose index of volume(CTDIvol) and images qualities were evaluated respectively.And the results were compared with traditional thoracic CT scanning(200mAs,15.31mGy).Results Compared with routine sequence,the exposure dose of singe-slice was decreased by 48.2% when the CARE Dose 4D technique was used(maximal decreasing 84%),CTDIvol was reduced about 32.98%(maximal reduction of 56.5%),there was statistically significant difference(P

Objective To evaluate the reliability and clinical application of measuring blood glucose and urea with electrodes and end reaction methods. Method Using the electrodes and end reaction methods, blood glucose and urea were measured respectively. The results and the relativity between the two methods were compared. Result Comparing the two methods for GLU and UREA, the correlative factors were 0.990 1 and 0.989 1 respectively. They had fairly good comparability. The linear ranges of their regression equations met the clinical needs. With favorable accuracy, the correlative factors were above 0.997 9. Conclusion The electrodes method was rapid and accurate, which was suitable for emergency tests. The ending reaction methods was fairly stable and has less interfering factors, which was fit for the routine use.

BACKGROUND: Studies concerning muscle-derived stem cell transplantation mainly focused on treatment of muscular dystrophy. There are no studies on muscle-derived stem cells for treatment of denervated amyotrophy combined therapy. OBJECTIVE: To explore effects of muscle-derived stem cell transplantation on denervated gastrocnemius atrophy. DESIGN, TIME AND SETTING: The randomized controlled animal study was performed at the Shenyang Medical College from February 2007 to July 2008. MATERIALS: A total of 19 adult healthy male Wistar rats were supplied by Animal Experimental Center, Shenyang Medical College. METHODS: Triceps brachii was sterilely collected from 3 rats, and muscle-derived stem cells were isolated in vitro, and purified by adherence method. The tibial nerve of right posterior limb was cut in remaining 16 rats to establish animal models of denervated gastrocnemius. In the cell transplantation group, 0.2 mL DAPI-labeled muscle-derived stem cell suspension was infused into the denervated gastrocnemius. The same volume of saline was injected into the model control group, for 4 weeks. MAIN OUTCOME MEASURES: Computer biological signal collection processing system was used to measure recovery rate of threshold intensity of gastrocnemius and the optimal intensity recovery rate. Electronic balance was utilized to calculate residual rate of wet weight. Image analysis system was used to measure residual rate of cross section area. RESULTS: Compared with the model control group, the recovery rate of threshold intensity of denervated gastrocnemius and the optimal intensity recovery rate were significantly lower in the cell transplantation group (P