Objective To research the microinvasive therapy for intraspinal tumors resection and study the ways for spinal function protection. Methods Twenty-four cases were detected by MR. The intraspinal tumors located variously and the size of them ranging from 0.9 cm to 3.0 cm. All the tumors underwent resection via Key-hole on mono-lamina and the clinical data were analyzed. Results After tumor pathological examination,the cases of neurofibroma,neurinoma,and spinal meningioma were 9,12 and 3 cases. respectively. All the patient recovered and they walked within one week after operation. No recurrenced on MR examination after 3 months and no spinal tube destruction on CT examination after 6 months. Conclusion The microinvasive therapy via Key-hole on mono-lamina is benefic to the totally resection of intraspinal tumors and also benific to the spinal function protection.

Objective To study the characteristics and differential diagnosis of obsolete pulmonary tuberculosis accompanied withperipheral lung carcinoma.Methods CT findings of obsolete pulmonary tuberculosis accompanied with peripheral lung carcioma in 20 cases were retrospectively analysed.Results In 20 patients ,12 cases were diagnosed correctly because of them with typical CT findings of peripheral lung carcinoma,while 8 cases were misdiagnosed because of atypical CT findings of lung carcinoma.Conclusion Obsolete pulmonary tuberculosis accompained with peripheral lung carcinoma is common seen,atypical lung carcnioma is easily misdiagnosed.

Objective To study the frequency and relationship with gestational age of fetal nucleated red blood cell (NRBC) in maternal peripheral blood. Methods Samples of peripheral blood in 44 women of 6~40 gestational weeks were collected to enrich the fetal nucleated red blood cells by discontinuous density gradient centrifugation. The isolated cells were made smears and counted under the microscope. NRBCs were found and retrieved using a micromanipulator under a microscope for PCR amplification of Y chromosome specific DNA to determine fetal sex. Results NRBCs were found in 17 out of 44 maternal samples, distributing from 9 to 26 gestational weeks The highest frequency of NRBC was found in 11~20 gestational age which reached to 76.5%(13/17). The amount of detected NRBCs ranged from 5/7ml to 30/7ml. Y chromosome 149bp was found in 7 cases and not in the other 10, which agreed to the actual fetal sexes. Conclusion The appropriate time to make a prenatal diagnosis using fetal nucleated red blood cells is in 11~20 gestational age.

Objective [WT5”BZ] To evaluate the effects of glycyl glutamine (Gly Gln) on the small intestinal graft. [WT5”HZ]Methods [WT5”BZ] Ten outbrid pigs undergoing small bowel autotransplantation were randomly divided into 2 groups. In STPN group, the animals received standard TPN devoid of Gly Gln,and in GTPN group,the animals received isonitrogenous (0 3?g?kg -1 ?d -1 )and isocaloric (nonprotein calory:137 9?kJ?kg -1 ?d -1 ) TPN with 2% Gly Gln.Animals were fed for 28 days. [WT5”HZ]Results [WT5”BZ] At the end of TPN,the mucosal contents of Gln (1 14?0 20)??mol/g tissue wet weight, protein (101?22)?mg/g and the villous contents of DNA (3 84?0 39) and RNA (3 31?0 14),the graft villous height (308?52) ?m, surface area (0 154?0 019) mm 2 and mucosal thickness(665?72) ?m were significantly higher in the GTPN group than that in STPN group ( P

CXCR4 has long been considered as the unique receptor of CXCL12,and CXCL12/CXCR4 axis plays a prominent role in tumorigenesis.However,a novel receptor for CXCL12,named CXCR7,has been recently identified and also plays an important role in tumorigenesis.This review summarized current studies regarding the functions of CXCR4 and CXCR7 in cancer and the recent therapeutic approaches that target these receptors or their ligands.

Objective:To analyze the content of Pb, Cd, Cu, As and Hg in freeze-dried Rehmannia from different habitats by FAAS and double channels AFS. Methods:The contents of Pb,Cd and Cu in Rehmannia from different habitats were directly deter-mined by FAAS, while the contents of As and Hg were determined by double channels AFS after pretreated with HNO3-HClO4 . Re-sults:It was shown that each element had good linear relationship with the correlation coefficient above 0. 997 4. The average recovery was 97. 88%-100. 90% with RSD of 0. 019% -4. 20% (n=6). Meanwhile, the concentrations of 5 harmful elements and heavy met-als in 4 batches of 10 samples were higher than the limits described in Chinese pharmacopoeia. Conclusion:The results measured by the method are considered to be accurate, quick and easy to be operated. It can be used to determine heavy metals and harmful ele-ments in freeze-dried Rehmannia from different habitats.

Objective To establish the basis of molecular systematics of Angelica from different areas of Gansu province so as to offer molecular basis of identification Gansu-produced Angelica with geoherbalism. Methods The ITS gene of Angelica was obtained by PCR and sequenced to be analyzed by DNAStar software to calculate the genetic distance among different specimens. Results The ITS sequence of Angelica between Min County and Zhang County of Gansu Province shows trivial differences with the range of 0.000 0～0.008 3. All 5 Min County-produced Angelica specimens have same bases at 586 site with "C" and 589 site with "T" with Zhang County-produced Angelica DG9 specimen, whereas the 586 site and 589 site bases of the other 3 Zhang County-produced Angelica specimens are "T" and "G" respectively. Conclusion The ITS sequence characteristics can be recognized as effective molecular marker of Angelica, and both 586 site base and 589 site base of the ITS sequence of Angelica can be recognized as identification bases of Min County-produced Angelica.

Objective To study the relationship between generalized tonic clonic epilepsy of different frequence seizure and erythrocyte ATPase activity.Methods We measured Na +/K + ATPase,Mg 2+ ATPase,Ca 2+ ATPase activity of 68 patients with generalized tonic clonic seizure who were divided into 3 groups by different frequence:Status epilepsy group (SE) for 26 cases,Frequency epilepsy (FE) group for 22 cases,epilepsy (EP) group for 20 cases and the normal controls for 35 cases.Results Erythrocyte Na +/K + ATPase in SE, FE group decreased,as compared with EP group,the normal control group, there was remar kable difference( P

Objective To study the effect of blood transfusion on earthquake injuries with crush syndrome in WenChuan earthquake.Methods The amount of red blood cell suspension given to the earthquake victims were studied. The transfusion effect, Hb concentrations, and capillary hemorrhage of tissues of the injuries were also observed.Results Two victims, who underwent amputation surgery and cerebral hemorrhage operation, received a relatively small amount of transfusion, and showed a good transfusion effect and prognosis. While the other 6 victims, who underwent amputation surgery, surgical debridement, or continuous renal replacement therapy, received a large amount of blood, and showed a bad transfusion effect and prognosis.Conclusion The amount of blood transfusion in patients with crush syndrome was correlated negatively with the outcome.

Objective To establish a method to isolate and cultivate rat bone marrow mesenchymal stem cells (BMSCs),and explore the feasibility of labeling in vitro,and tracing in vivo,BMSCs with chloromethyl-benzamidodialkylcarbocyanine (CM-Dil). Methods BMSCs were obtained and subsequently cultured with whole bone marrow cell culture system,and the third generation of BMSCs was harvested. The positive rates of CD34,CD44 and CD29 were detected by flow cytometry. CM-Dil was used to label BMSCs in vitro and the efficiency of labeling after 24 hours and at day 21 and 30 were examined under fluorescent microscope. Moreover,the growth curves were sketched to determine the negative effects of CM-Dil on the vitality and proliferation of BMSCs cultured in vitro. Rat model of focal cerebral ischemia was reproduced,CM-Dil labeled BMSCs were implanted into corpus striatum of rats' brain with computer-guided stereotaxis thereafter. Brain tissues were obtained to prepare frozen sections at 7th,14th and 21st day post-implantation,and the survival and distribution of labeled cells were observed with fluorescent microscopy. Results The third generation passage of cultured BMSCs grew orderly,with shape of desmocytes,and homogeneous in morphology. The positive rates of CD34,CD44 and CD29 expressions in BMSCs were 1.71%,80.32% and 84.89%,respectively. Red fluorescence was observed in CM-Dil labeled BMSCs in vivo 24 hours after labeling,with a positive rate of 100%. The fluorescence intensity of passage cultured BMSCs observed on day 21 was similar to that observed at 24 hours after labeling,but diminished on day 30. The growth,proliferation and morphology of BMSCs,were not influenced by CM-Dil labeling. On day 7,14 and 21 post-implantation,BMSCs decreased in quantity,appearing in oval or irregular shapes,and most of the cells were found around the needle tract,with a tendency of diffusion to peripheral area. Conclusion High purity of BMSCs may be obtained with whole bone marrow cell culture system from bone marrow of rats. CM-Dil labeling is easy to handle and effective,with no cytotoxicity to cells. The latest labeling period of CM-Dil is 21 days in vitro,therefore it seems to be an effective method for in vivo tracing of BMSCs in brain after implantation.