Forecasting ; Quantum Theory ; Toxicology ; trends

OBJECTIVE:To establish a method for simultaneous determination of puerarin,berberine hydrochloride and ba-icalin in Erxieting syrup. METHODS:HPLC was performed on the column of Kromasil-C18 with mobile phase of methanol-0.2%Phosphoric acid solution (gradient elution) at flow rate of 1.0 ml/min,column temperature was 30 ℃,detection wavelength was 275 nm,and the volume injection was 10 μl. RESULTS:The linear was 10.43-83.44 μg/ml for puerarin,10.71-85.68 μg/ml for ber-berine hydrochloride and 12.58-100.64 μg/ml for baicalin(r≥0.999 3);RSDs of precision,stability and reproduciblity tests were lower than 2%;recoveries were 98.25%-100.87%(RSD=0.92%,n=6),99.41%-101.40%(RSD=0.68%,n=6)and 98.13%-99.76%(RSD=0.57%,n=6),respectively. CONCLUSIONS:The method is simple,reliable,can be used for the quality control of Erxiet-ing syrup.

Objective To evaluate the intervention efficacy of hand hygiene of health care workers(HCWs)in a hospital before and after the comprehensive interventions were taken. Methods The baseline investigation stage was in April-June,2013 ,intervention was performed from July,2013,post-intervention efficacy evaluation stage was in October-December ,2013. Hand hygiene compliance was observed by investigators ,and questionnaires were filled out. Results After multiple interventions were taken,hand hygiene compliance rate of HCWs increased from 38.02% to 53.86% (χ2= 181.82,P<0.05),post-intervention compliance rates of hand hygiene improved significantly in departments of internal medicine,surgery and intensive care unit (all P<0.05).After intervention,the overall hand hy-giene compliance rate at different work opportunities rose from 41.87% (1 740/4 156)to 53.45% (2 044/3 824)(χ2=107.2,P<0.05);except sterile manipulation,the other 4 manipulation opportunities were all higher than pre-intervention (all P<0.05);HCWs of different professions were all higher than pre-intervention(all P<0.05).Conclusion Active hand hygiene intervention can effectively improve hand hygiene compliance of HCWs.

Objective To compare the efficacy of laparoscopic radical hysterectomy and open surgery with early stage cervical cancer in elderly patients.Methods Performed a retrospective study of 195 cases underwent surgical treatment of early stage cervical cancer in elderly patients.According to different approach,the patients were divided into laparoscopic group (66 cases) and open group (129 cases).The operation time,intraoperative blood loss,number of resected lymph nodes,bowel function recovery time,postoperative hospital stay and postoperative complications was compared between two groups.Results The operation time,intraoperative blood loss,bowel function recovery time,postoperative hospital stay in laparoscopic group were significantly shorter than those in open group [(237.6 ±46.9) min vs.(259.3 ± 76.7) min,(214.7 ± 104.8) ml vs.(283.4 ± 137.2) ml,(1.9 ± 1.2) d vs.(2.8 ± 1.5) d,(4.1 ± 3.5) d vs.(6.7 ± 1.4) d,P ＜ 0.05 or ＜ 0.01].There was no significant difference in number of resected lymph nodes and postoperative complications between laparoscopic group and open group (P ＞ 0.05).Conclusion Laparoscopic radical hysterectomy is both safe and effective in elderly patients with early stage cervical cancer.

Objective To obeserve the effects of acute hypervolemic hemodilution(AHH) with hypertonic .sodium chloride hydroxyethyl starch 40(HSH 40) on hemodynamics and fluid balance in patients under general anesthesia.Methods Fifty patients undergoing radical surgery for gastral cancer under general anesthesia were randomly divided into 2 groups with 25 patients each.Acute hypervolemic bemodilution (AHH) was performed with HSH 40 6 ml/kg in group A or with hydroxyethyl statch(HES) 6 ml/kg in group,which was infused within 30 minuts.HR,MAP,CVP were recorded before(T_0),at 30 min (T_1),60 min (T_2) after infusionand and the end of operation (T_3).The amounts of bleeding,HSH 40 and HES and urine output were recorded as well.Results There were no significant diferences in HR and MAP between two groups at all time points.CVP was sighificantly higher at T_1-T_3 than that at To in two groups.The urine output was more in groups A than that in group B(P<0.05).Conclusion AHH with HSH 40 can effectively expand blood vlume and increase urine output in surgical patients under general anesthesia.

OBJECTIVE:To test the static balance function of boys aged from 6 to 8 years and then to establish a system of evaluating the static balance function.METHODS:A total of 388 boys aged from 6 to 8 years were randomly selected from 16 kindergartens of 8 counties(cities)in 4regions of Hebei Province.The inclusive cdteria were as follows:no systematic training of sports,no myopia,normal body shape and no obvious obesity.The balance force-measuring plates made by Hefei Institute of Intelligent of Chinese Academy of Sciences,computer and the supporting software were adopted.The subjects received an indoor test of 6 testing postures,namely,standing on two feet with eyes open(and closed),standing on left leg with eyes open(and closed),standing on right leg with eyes open(and closed).Six indexes relating to balance abilities including the mean of X direction,the mean of Y direction,R value,track length per unit time and track location variance of the envelop area.RESULTS:The original screening of all indexes showed that 6 kinds of postures reduced to 4 and that indexes reduced from 36 to 24.The second screening with cluster analysis which was conducted to the 4 kinds of postures showed that 24 indexes reduced to 17 when selecting typical indexes from each cluster.The third screening with factor analysis showed that 7 out of the 17 indexes were remained.Seven representative indexes corresponding to these 7 factors were concluded and considered as the comprehensive evaluation indexes of the static balance.The comprehensive static balance evaluation index systems for 6-year-old.7-year-old and 8-year-old boys were established respectively.CONCLUSION:Three systems for evaluating the static balance function of the boys aged from 6 to 8 years are established successfully based on 7 indexes.The evaluation of static balance function should be based on the comprehensive evaluation of multiple indexes.The evaluation indexes for 6-year-old,7-year-old and 8-year-old boys are identical in number,but different in content.Accordingly,a specific evaluation index system should be established for them separately.

Objective To understand the incidence trend of ventilator -associated pneumonia(VAP)in ICU and to evaluate the effects of the interventions on the incidence of VAP,so as to provide scientific evidences for the prevention of VAP.Methods A study of 149 patients with VAP occurred in the 3 710 patients of ICU before and after intervention during 7 /2010 -12 /2014 in Wujin People's Hospital,Changzhou city was conducted.It was divided into two stages:baseline survey and stem expectations,VAP interventions included:training and education,chlorhexidine oral care and maintain appropriate endotracheal tube cuff pressure,elevated head of bed to up to 30°and improved hand hygiene.Hospital infection control professionals collected the number of patients,basic information,length of hospital stay,days of receiving mechanical ventilation and the VAP incidence data in ICU.And then change of the VAP incidence of the intervention before and after the intervention was compared and evaluation of the effect of combined intervention measures.Results Prospective data from 3 710 patients hospitalized in ICU were collected for an aggregate of 14 831 days,the utilization rate of ventilator was 55.2%.The occurrence of VAP was 149 cases.The average rate of VAP was 18.2 cases per 1 000 ventilator -day.VAP was associated with a crude mortality of 7.56%and with increased ICU LOS of 13.2d.As the results of the interventions,the rate of VAP was decreased from 52.38 /1 000 during 7 /2010 -6 /2011 to 5.71 /1 000 during 1 /2014 -12 /2014.Conclusion The rate of VAP in the inves-tigated ICU has led to a decreased incidence of VAP under the comprehensive interventions.

This study investigated the conjoined cellular oxidative damage of human embryo kidney 293T (HEK293T) cells induced by cadmium chloride (CdCl(2)) and nanometer titanium dioxide (nano-TiO(2)). RT-PCR technique was used to detect the expressions of Heme oxygenase-1 (HO-1) and 8-oxoguanine DNA glycosylase (OGG1). The activities of superoxide dismutase (SOD) and catalase enzyme (CAT) and concentrations of reactive oxygen species (ROS) and maldondialdehyde (MDA) were measured by different approaches. The results showed that CdCl(2) and nano-TiO(2) at a low concentration of 0.75 total toxic unit (TU) exerted an additive effects on HO-1 gene expression, CAT activities and MDA concentrations. When the total TU was increased to 1 or 1.25 TU, the interaction was synergetic. Moreover, the mixture with high proportion of CdCl(2) produced an additive effect on the OGG1 gene expression, and the interaction was changed to be synergetic when the concentration of CdCl(2) was lower than or equal to that of nano-TiO(2). Synergetic effects of CdCl(2) and nano-TiO(2) on cellular oxidative damage of HEK293T cells were found as indicated by the changes in the SOD activities and ROS concentrations. It was concluded that CdCl(2) and nano-TiO(2) exerts synergistic effects on the cellular oxidative damage of HEK293T cells, and the sensitivity of these indicators of oxidative damage varies with the proportion of CdCl(2) and nano-TiO(2) in the mixture.

[Objective]To explore the clinical outcome of anterior cervical decompression for severe cervical spondylotic myelopathy with ossification of posterior longitudinal ligament. [Method]Thirty-three patients with severe cervical spondylotic myelopathy and continuous OPLL underwent posterior longitude ligament resection and floating in anterior cervical decompression.The intervertabral space were stabilized by autoilum graft or titanium cage and fixed with windows plates. [Result]All patients were followed up for 8 to 45 months,mean 22 months.Preoperative JOA scores were 6.7,and the postoperative JOA scores were 10.1 and 10.7(evaluated in 3 months and 12 months after surgery),the mean amelioration rate was 72.7% and 78.8%.Of these 33 patients,no severe complications such as cord or vertebral artery injury occurred. [Conclusion]Posterior longitude ligament resection and floating in anterior cervical decompression is a safe and effective treatment for severe cervical spondylotic myelopathy with ossification of posterior longitudinal ligament.

0.05).Compared with the control group,the weight and viscera coefficient of testis in high dosage group,the sperm counts of epididymides in medium and high dosage experiment groups were all decreased significantly(P0.05).The activities of succinate dehydrogenase(SDH) and nitric oxide synthase(NOS) of testis in high dosage group,the activities of acid phosphatase(ACP),alkaline phosphatase(AKP) and Ca-Mg-ATPase of testis in medium and high doseage groups were all decreased significantly compared with the control group(P