Drug pair is the basic unit of compatibility of formulas.Through selecting the prescriptions of treating diseases of the liver in "Treatise on Cold Pathogenic Diseases" and "Synopsis of Golden Chamber",we,according to therapeutic methods,classify the commonly used drug pairs in treatment of hepatic disease of ZHANG Zhong-jing into eight kinds:regulating the flow of liver-qi,promoting blood circulation by removing blood stasis,dispersing pathogenic wind and calming liver wind,removing jaundice,clearing away liver-heat,expelling liver-cold and warming the liver,nourishing the liver,and harmonizing liver-spleen.And we try to find the compatibility law.

Objective To explore the factors of medical anthropology related to malaria control among Wa Ethnic minority in Ximeng County, Yunnan Province. Methods The study was conducted by the combination of anthropology and epidemiology, qualitative and quantitative, statistical analysis and description. Results Wa people considered malaria as one of three major diseases in the community. In Wa Ethnical language, the word of malaria, “Saihui” (Sai-illness and pain, Hui-being attacked quickly), does not mean of fever. About one third of Wa people had not thought fever as one of malaria symptoms. Most of Wa people only knew vivax malaria, but not falciparum malaria. Only 32.7% of people could connect malaria with mosquitoes, and a few of them could confirm mosquitoes as the only malaria vector. The proportions of sound knowledge of malaria causes, prevention and treatment seeking assessed were lower than 25.0%. As a result of multivariate logistic regression, 3 kinds of malaria knowledge were closely correlated each other, and the connection between causal knowledge and prevention (adjusted OR: 36.610, 95%CI: 10.242-130.866, P

Mitochondrial DNA mutations are one of the most important causes of sensorineural hearing loss. A1555G and C1494T mutations of mitochondrial 12S rRNA gene are the molecular basis for aminoglycoside hyper- sensitivity and can lead to aminoglycoside-induced hearing loss. Primary mutations in tRNA such as tRNA(Ser(UCN))7472insC are associated with syndromic hearing loss. While other mutations such as tRNA"(Se(UCN) )G7444A were considered synergy with the primary RNA mutations, modulating the phenotypic manifestation. This review de- scribes a detailed summary of hearing loss associated with mtDNA mutations and/or aminoglycoside antibiotics, and provides the possible molecular mechanisms in deafness expression.
Aminoglycosides ; adverse effects ; Anti-Bacterial Agents ; adverse effects ; DNA, Mitochondrial ; genetics ; Hearing Loss, Sensorineural ; chemically induced ; genetics ; Humans ; Mutation ; RNA, Ribosomal ; genetics

Objective To study the expression of IL 1?,TNF ? and nitric oxide (NO) in the cervical intervertebral disc and their roles in the pathogenesis of cervical spondylotic myelopathy (CSM). Methods Fifteen cervical discs were obtained from patients of CSM undergoing anterior disc surgery. Six cervical discs specimens were obtained in the surgical operation with patients with traumatic cervical injury served as the control. The specimens were analyzed for IL 1?、TNF ? by use of ELISA method, NO was measured by optical density assays. Results The contents of IL 1?,TNF ? and NO in disc of CSM patients increased significantly when compared with the control group. Conclusion The results of the present study indicated that IL 1?,TNF ? and NO may be involved in the pathogenesis of CSM.

Drug resistance is major obstacles in the successful treatment of malignant solid tumors. Multiple mechanisms involeve in development of cancer drug resistance. Recent research suggests dysregulation of microRNAs is associated with cancer drug resistance. The profiles of microRNAs in drug resistance cancer cells or tissues are different with sensitivity cells or tissues in various solid tumors. Restoring microRNAs could improve chemosensitivity of cancer cells. MicroRNAs expression profiles may provide a critical link for understanding mechanisms involved in chemoresistance. We can also find a specific marker for screening chemosensitivity patients through identification of the microRNAs patterns of drug resistance cells or tissues.

Objective To explore the expression and significance of MTSS1 ( metastasis suppressor 1) in hepatocellular carcinoma.Methods MTSS1 expression was detected by immunohistochemistry in hepatocellular carcinoma, liver cirrhosis and normal liver tissues.Single-factor analysis was used to study the relationship with clinicopathological factor.Correlations between MTSS1 expression and TNM stage were analyzed with Spearman rank correlation analysis.Postoperative 5-year survival was evaluated using Kaplan-Meier survival curve analysis.Results The expression of MTSS1 in hepatocellular carcinoma was higher than normal liver tissue ( U = 168.000, P ＜ 0.05), and liver cirrhotic tissue ( U = 106.000, P ＜ 0.05); MTSS1 expression was correlated with TNM stage of hepatocellular carcinoma patients, lymph vascular invasion and tumor capsule ( separately U = 259.000, 258.500, 202.000, all P ＜ 0.05).MTSS1 expression in hepatocellular carcinoma was not correlated with patients age, gender, tumor size, AFP level, and hepatitis B surface antigen.MTSS1 expression and TNM stage of liver cancer patients was negatively correlated ( rs = - 0.383 , P ＜ 0.05 ).Postoperative 5-year survival of hepatocellular carcinoma patients with MTSS1 positive expression was significantly poorer than patients with negative and weakly positive expression (respectively 34.1% and 52.3% , x2 =6.386, P ＜ 0.05).Conclusions MTSS1 high expression may play an important role in the early hepatocellular carcinoma progression, indicating a poor prognosis.

Objective To investigate the effect of continuous positive airway pressure therapy (CPAP) on heart rhythm and blood pressure variability in primary hypertensive patients with obstructive sleep apnea/hypopnea syndrome (OSAHS).Methods Ninety seven patients with OSAHS and primary hypertension were randomly divided into treatment group (n =49) and control group (n =48).Patients in control group receive antihypertensive medication only,while patients in treatment group received antihypertensive medication in combination with CPAP therapy for 4 weeks.The dynamic electrocardiogram (ESG) and ambulatory blood pressure for 24 h were measured before and after treatment in both groups.Results After CPAP treatment,the night index of standard deviation of the average NN intervals (SDANNniGHt) in treatment group was (89.0 ± 21.4) ms,while the day and night index of square root of the mean squared differences of successive NN intervals (rMSSDday and rMSSDnight) was (35.9 ± 17.2) ms and (45.7 ± 18.3) ms respectively.Compared with those before treatment,the index of SDANNnight was decreased and the indexes of rMSSDday and rMSSDnight were increased (P ＜ 0.01).Compared with control group,the SDANNnight was decreased,and the rMSSDday and rMSSDnight were increased (P ＜ 0.01,P ＜ 0.05).After CPAP treatment,the night index of low-frequency power (LFnight) in treatment group was (15.3 ± 5.7) ms,which was lower than that before treatment,and the night index of high-frequency power (HFnigh) was (16.3-± 8.1)ms,which was higher than that before treatment (P ＜ 0.01);the LFnight in treatment group was decreased and the HFnight was increased (P ＜ 0.01).After CPAP treatment,the day and night indexes of systolic blood pressure (SBPday and SBPnight) and the night indexes of diastolic blood pressure (DBPnigh) t in treatment group were (136.1 ± 13.4) mmHg,(128.7 ± 9.8) mmHg (1 mmHg =0.133 kPa),and(83.7 ±7.2)mmHg respectivley,which were all lower than those in control group (P ＜ 0.05 or P ＜ 0.01).The incidences of morning peak blood pressure and non-dipper hypertension in control group were significantly higher than those in treatment group (P ＜ 0.01).Conclusion CPAP therapy can effectively adjust the heart rate and blood pressure levels in patients with OSAHS and primary hypertension,especially in the night.

A method for the infrared spectrophotometric determination of total ph-ospholipids in seed of Juglans regia and acinus of Citrullus vulgaris was reported. The average recovery of total phospholipid in this test was 97.2%, the CV was 1.66%. The contents of total phospholipid in seed of Juglans regia and acinus of Citrullus vulgaris were 438.46-528.85mg% and 241.78 -247.47mg% respectively. The rapid quantitative analysis of the distribution of various phospholipid components in these two specimens was carried out by using thin layer chromatographic scanning and the corrective method of absorbance proportional coefficient. The results showed that the main phospholipid components in both specimens were PC and PE. The fatty acid composition of the total phospholipids of both specimens were analysed by GC-MS-DS (gas chromatography-mass spectrometry-data system).

Objectives To learn the bednet use and influential factors among Wa and Lahu ethnics,and further to explore feasible ways of promoting the use of bednets and insecticide-treated nets (ITNs). Methods Questionnaire surveys and statistical analysis with EpiInfo 2000 software package were carried out. Results Only did 2.8% [95% confidence intervals(95%CI):1.2%-5.4%] of Wa and 4.8 % (95%CI:2.8%-8.1%) of Lahu ethnic members use bednets frequently or sometimes. Bednets were not available for 67.7% (95% CI:62.0%-73.1%) of Wa and 59.7% (95% CI:53.1%- 66.1%) of Lahu ethnic peoples. Most people who had bednets did not often use them for anti-mosquitoes. Results of multivariate logistic regression confirmed significant correlation between bednet use and knowledge of malaria prevention. Conclusion The coverage and use rates are very low in the two ethnical minorities. Health education, communication and social marketing are the strategies for promoting the usage of bednet and ITNs.

AIM:Many studies have documented an anabolic effect of hydroxymethylglutaryl coenzyme A (HMG-CoA) reductase inhibitors, statins, on undisturbed bone. Reports of their effects on fractured skeleton were limited. A study was therefore conducted to check the effects of statins on fracture healing. METHODS: Simvastatin (10 mg?kg-1?d-1) was injected subcutaneously to tissue overlying the site of fractured tibiae of ovariectomized rats for a treatment period of 5 d. Vehicle reagent was used as control. Healing quality was evaluated at 1, 2 and 4 weeks after fracture. RESULTS: Compared with vehicle group, callus cross section area in simvastatin treated rats were significantly enlarged by 21.3% (P