BACKGROUND: The far-infrared ceramic microspheres developed by the State Key Laboratory of “new ceramics and fine technology” of School of Materials, Tsinghua University, are made with ceramic colloidal injection molding technology. It is a new type of physical therapy material in the field of sports rehabilitation and daily health care. With its unique small circular structure, it can completely cover the damaged parts such as feet, waist, thigh, etc. to achieve comprehensive stimulation intervention. OBJECTIVE: To investigate the effect of far-infrared ceramic microspheres on pain Intensity after muscle injury. METHODS: Thirty college students aged 18-21 years who met the diagnosis standard of posterior femoral muscle group injury were included in this study. All of them provided informed consent. They were randomly divided into three groups, with 10 students per group. Students in the massage group underwent massage therapy. Students in the far-infrared instrument group were treated with ordinary far-infrared therapeutic apparatus. Students in the far-infrared ceramic microsphere Intervention group underwent far-infrared ceramic microsphere intervention. All treatments lasted 2 successive weeks. Before and 3, 7, and 14 days after treatment, McGill pain scale score (including pain rating index, visual analogue score and present pain intensity) was measured and compared within and between groups. RESULTS AND CONCLUSION: (1) Before treatment, there were no significant differences in pain rating index (sensory, affective, and total pain rating index scores), visual analogue score and present pain intensity between three groups (P > 0.05). (2) At 3 days of treatment, pain rating index, visual analogue score and present pain intensity score in the far-infrared ceramic microsphere intervention group were significantly lower compared with the massage and far-infrared instrument groups (P < 0.05). After 7 and 14 days of treatment, each studied indicator in the far-infrared ceramic microsphere intervention group was highly significantly lower compared with the other two groups (P < 0.01). (3) At 3 days of treatment, score of each pain indicator in the far-infrared ceramic microsphere intervention group was significantly lower compared with before treatment (P < 0.05), and it was significantly decreased compared with that measured concurrently in the other two groups (P < 0.05). (4) At 7 days of treatment, score of each pain indicator in the far-infrared ceramic microsphere intervention group was significantly lower compared with before treatment (P < 0.01), and it was significantly decreased compared with that measured concurrently in the other two groups (P < 0.05). (5) After 14 days of treatment, score of each pain indicator in the massage and far-infrared instrument groups was significantly lower compared with before treatment (P < 0.05). After 14 days of treatment, score of each pain indicator in the far-infrared ceramic microsphere intervention group was highly significantly lower compared with before treatment (P < 0.01). After 14 days of treatment, score of each pain indicator in the far-infrared ceramic microsphere intervention group was significantly lower compared with the other two groups. These findings suggest that far-infrared ceramic microsphere intervention can effectively reduce the degree of posterior femoral muscle group Injury and effectively promote the recovery of muscle injury.

OBJECTIVE:To determinate the serum concentration of methotrexate by HPLC. METHODS:The samples were separated on a Waters C18 column at a column temperature of 30 ℃. The mobile phase consisted of acetonitrile-phosphate buffer(pH 7.2,12∶88) at a flow rate of 1.0 mL?min-1. The detection wavelength was set at 303 nm. RESULTS:The linear range of methotrexate was 0.5～80 ?g?mL-1(r=0.999 4). The methodological recovery rates were all above 90%; the intraday RSD was 3.03 %～3.52 % and the inter-day RSD was 2.57%～4.05%. CONCLUSION:The method is proved to be simple,rapid,accurate and sensitive,and it is suitable for the determination of serum concentration and pharmacokinetic study of methotrexate.

Objective To investigate the incidence of the post-stroke emotion disorder, the relationship between post-stroke emotion disorder and the location of stroke, and the effects of Paroxetine combined with psychotherapy in treating post-stroke emotion disorder. Methods 181 patients with acute brain stroke were assessed by SDS and SAS. Of these patients, 54 patients met the criteria of depression and anxiety after acute brain stroke. These 54 patients were divided into 2 groups randomly. They were treated with routine anti-stroke medication, routine anti-stroke medication with Paroxetine combined with psychotherapy for 6 weeks, respectively. The rating scales such as SSS, HAMD, HAMA, TESS and so on were used to evaluate the improvement of symptoms and side effects. Results According to the evaluation of SDS or SAS, there were 81 patients (44.75%) with post-stroke emotion disorder. The rate of post-stroke depression with anxiety was 66.67%. Post-stroke depression with anxiety was related to the location of brain stroke, such as frontal cortex,left cerebral hemisphere and basal ganglia (P

respectively)was significantly higher than that for digital anterograde venography (0.684 and 0.587,P =0.026,respectively).Conclusion Diagnostic accuracy and detection rate of residuaI or recurrent varicose veins with CT phlebography are found to be more superior than that with digital anterograde venography which has high guidance value in preoperation and assesses the efficacy of post-surgery treatment.

Objective: To observe the clinical effects of scalp acupuncture and body acupuncture on post-stroke ataxia. Methods: 124 cases of post-stroke ataxia were treated with scalp and body acupuncture to observe the changes of blood velocity in the blood vessels of the brain and the total effects after treatment. Results: The total effective rate was 93.5% based on the result of Berg Balance scale, and was 91.1% on the activities of daily living (ADL) index. The blood velocity of the vertebrobasilar artery was higher after the treatment compared to before treatment (P＜0.05). Conclusion: Acupuncture had good clinical effects.

AIM: To explore the effects of glucocorticoids on fracture healing in a rat model of tibial fracture. METHODS: Sixty three months old female SD rats were divided into control and glucocorticoid-treated group. A glucocorticoid-induced osteoporosis model was established by intramuscular injection of prednisolone acetate (5 mg·kg~(-1)·d~(-1) for 3 weeks), in which the tibial was osteotomized by a wire saw as fracture healing model and internal fixed with a Kirschner pin. The rats were scarified at different time points after operation. The callus formation was monitored over a period of 6 weeks by histological method, bone mineral density (BMD) detection and biomechanical examination. Western blotting was used to measure the expression of type II collagen. RESULTS: A glucocorticoid-induced osteoporosis model was successfully established and conformed by BMD measurement. The formation of primary callus was observed in both groups 3 days after fracture. At 2 weeks after injury, the glucocorticoid-treated group had a lower BMD and less cartilage matrix as compared to control group. An increase in bone callus and chondrogenesis was observed at 4 to 6 weeks after fracture in glucocorticoid-treated group as compared to control group. The expression of type II collagen was delayed in glucocorticoid-treated group. Biomechanical measurement showed that the actual maximum load was increased by 35.8% in control group as compared to glucocorticoid-treated group at 6th week. CONCLUSION: These results indicate that chondrogenesis and transformation from cartilage callus to bony callus are delayed by glucocorticoids. The retardation of collagen Ⅱ production may be the reason for the inhibition of fracture healing.

10.3969/j.issn.2095-4344.2013.26.004

Purpose To discuss the value of MRI in the diagnosis of placenta implantation abnormality, and to explore preliminarily the relationship between MRI signs and types of placenta implantation abnormality. Materials and Methods The clinical preoperative data and postnatal pathological findings of 54 women at high risk of placenta accreta were collected. All the patients undertook the conventional pelvic MRI examination. The scanning sequences mainly included: sagittal, coronal and axial T2-weighted imaging-turbo spin echo, balance fast field echo. The MRI images were observed and the areas which showed low signal in all the three directions on T2WI were measured. Then the correlation between the areas of low signal on T2WI in placental and the types of placenta implantation abnormality was analyzed. Results The incidence of placenta implantation abnormality was 64.8% in our research (35/54). The main MRI signs were low signal on T2WI (68.5%, 37/54) and heterogeneous signal in placenta (57.4%, 31/54); the main sign of placenta percreta was tenting bladder (75.0%, 6/8). The types of placenta implantation abnormality were positively correlated with the areas of low signal on T2WI (r=0.454, P<0.05). Conclusion Pregnant women at risk of placenta accreta should be evaluated with imaging examinations, particularly with MRI scanning, to improve disease detection rate. The typical indirect signs of placenta implantation abnormality are low signal on T2WI and heterogeneous signal in placenta. The larger size of low signal area on T2WI in placenta, the deeper implantation of placenta.

After the reform from "4+1" to "3+2",an undergraduate teaching model of clinical medicine,has been implemented in our university,higher demands are set on the training of students' abilities and creativity.This paper explored the basic medicine teaching from the perspectives of creative concepts,teaching staff,curriculum design and practice.

Objective To study the influence of combined spinal-epidural anesthesia on perioperative coagulation function,RAAS activity and postoperative analgesia effect in parturients with cesarean section.Methods One hundred and eighteen parturients of cesarean section in our hospital from June 2013 to January 2016 were collected and divided into the observation group and control group according to the random number table method,59 cases in each group.The observation group received the combined spinal-epidural anesthesia and the control group received epidural anesthesia.The coagulation function indicators on preoperative 1 d (T0),at 10 min before operation end(T1) and postoperative 6 h (T2) were detected by adopting the automatic blood coagulation analyzer,the renin angiotensin aldosterone system (RAAS) function indices were detectd by radioimmunoassay.The pain indicators at postoperative 6 h(T2),12 h (T3) were detected by the pain threshold test instrument.Results The levels of prothrombin time(PT),activated partial thromboplastin time (APTT) and thrombin time (TT) at T1 and T2 in the observation group were significantly higher than those in the control group,while the PTA level was lower than that in the control group(P<0.05);serum RAAS indices such as (renin),angiotensin Ⅱ(ANG II) aldosterone(ALD) in the observation group were lower than those in the control group(P<0.05).The VAS score at T2,T3 in the observation group was lower than that in the control group,while the pain threshold and pain tolerance threshold levels in the observation group were higher than those in the control group(P<0.05).Conclusion Combined spinal-epidural anesthesia can reduce the blood coagulation and RAAS activation caused by cesarean section trauma,and the effect of postoperative analgesia is more significant.