Objective To examine the expression change of HIF-1α and VEGF in protein levels and the corelation of them in Q8C939 cell line under chemical hypoxia induced by CoCl2 in vitro. Methods ELISA and immunohistochemical technique were used to examine the change of VEGF and HIF-1α protein level in QBC-939 line in different concentrations (200, 150, 100, 50, 0 μmol/L) of COCl2 and different periods (6 h, 12 h, 24 h, 48 h) in hypoxia. Results CoCl2 can up-regulate the expression of HIF-1α and VEGF at least partly in a dose and time dependent pattern. ELISA demonstrated that the level of VEGF protein in different concentrations and different hypoxia periods, the result showed that the VEGF protein was in different concentration groups over contrast group (P<0.05). The VEGF protein also increased continuously with the growing of hypoxia time (P<0.05). Immunohistochemical detection of concentrations of different groups, HIF-1α, VEGF protein in the average gray compared with the control group was different(P<0.05). Different time periods of hypoxia HIF-1α, VEGF protein in the average gray, any two group comparisons have difference (P<0.05). Different concentrations, the normal control group, different time period HIF-1α and VEGF protein expression of relevance were analyzed. The correlation coefficient was 0.830, P <0.01, 0.909, P <0.01. Conclusion In QBC939 cell line of hypoxia, there is up-regulation HIF-1α and VEGF.HIF-1α regulated the expression of VEGF.

Objective To evaluate the efficacy of transurethral pneumatic ballistic lithotripsy via outer sheath of ureterorenoscope for bladder calculi. Methods From March 2003 to May 2007, 32 patients with bladder calculi were treated by transurethral pneumatic ballistic lithotripsy via outer sheath of ureterorenoscope. Results The operation was completed in all the patients without conversion to open surgery. Transurethral prostatectomy (TURP) was carried out on 22 patients who were complicated with benign prostatic hyperplasia (BPH). Four patients received cystotrachelotomy because of bladder-neck contracture, and 4 underwent dilation of the urethra owning to urethral stenosis. Simple pneumatic ballistic lithotripsy was performed on two patients. The operation time was 25 to 90 minutes with a mean of (45?25) minutes. No patient had residual stones or perforation of bladder. One patient developed urethral stenosis after the operation. Conclusions Transurethral pneumatic ballistic lithotripsy via outer sheath of ureterorenoscope is a safe, less traumatic treatment for patients with bladder calculi with few complications. The method is especially suitable for patients with BPH.

ObjectiveTo evaluate the effect and safety of dezocine and sufentanil in intravenous analgesia for postoperative pain. Methods60 patients with ASA Ⅰ～Ⅲ,underwent general anaesthesia operation were divided into two groups randomly,with 30 cases for each group:dezocine 0.6mg/kg and sufentanil 0.6mg/kg was applied to PCIA in group A and B.PCIA was administered with subsequent bolus of 1 ml with lockout time 15 minutes and background infusion of 2ml/h.The analgesic effect was evaluated by VAS,BCS and Ramsay at 1、2、4、12、24、48 hours after operation.The changes of vital sign were also assessed. ResultsThere was no significant difference between two groups in analgesia effect(all P＞0.05)and adverse reaction incidence rate(P＞0.05). ConclusionThe effect of dezocine in intravenous analgesia for postoperative pain could be as satisfaction as that of sufentainyl.

This article was aimed to study the therapeutic effect of Chinese medicine Dai-Xie-A n (DXA) granules a-mong aging rats of metabolic syndrome (MS) in order to evaluate relative indicators and the pathophysiological role in neurons and microvascular injury among aging MS rats. Aging MS rat model was established by feeding high-fat diet and the established model was evaluated. Under the guidance of traditional Chinese medicine (TCM) theory, metabol-ic indexes, such as general state of health, glycolipid metabolism, insulin sensitivity, were explored in order to ob-serve effects on brain neurons and microvascular injury among aging MS rats. The results showed that DXA granules can improve glycolipid metabolism among aging MS rats, decrease TC, TG, TC/HDL, decrease the level of blood sug-ar and serum insulin, improve insulin resistance, decrease abdominal fat accumulation, reduce neuronal apoptosis, and increase microvascular angiogenesis. These effects are similar to rosiglitazone. It was concluded that DXA gran-ules can ameliorate glycolipid disorders, protect brain neurons and microvascular injury of aging MS rats.

Based on the formation and developing course of the system of diagnosis and treatment of Traditional Chinese Medicine(TCM),to explain three modes of diagnosis and treatment,including differential diagnosis of diseases therapy,determination of treatment based in pathogenesis obtained through differentiation of symptoms and signs,illness-syndrome coalescent.The latter one consist of the mode of illness-syndrome coalescent of TCM,which is based on diagnosing disease in TCM,to carry out determination of treatment based in pathogenesis obtained through differentiation of symptoms and signs and that based on diagnosing disease in modern medicine,to carry out determination of treatment based in pathogenesis obtained through differentiation of symptoms and signs of TCM.Meanwhile,to approach the advantages and flaws among different modes of diagnosis and treatment,accordingly we could offer theory evidences for elevating the level of curing disease of TCM.

BACKGROUND: Unilateral spinal epidural anesthesia with ropivacaine can reduce the perioperative pain in the elderly undergoing hip or knee arthroplasty, but choosing which concentration of small dose hypobaric ropivacaine is an issue of concern.OBJECTIVE: To explore the anesthetic and analgesic effects of unilateral spinal epidural anesthesia with different concentrations of low dose hypobaric ropivacaine in senile knee or hip replacement.METHODS: Ninety patients undergoing knee or hip replacement in the Henan Province Hospital of TCM from August 2014 to December 2015 were enrolled, and randomized into 0.15%, 0.5% and 0.25% ropivacaine groups (n=30 per group), followed by spinal epidural anesthesia at L3-4 or L2-3 lumbar interspace, respectively.RESULTS AND CONCLUSION: (1) The order of the flat subsided time and motor block recovery time was as follows:0.15% ropivacaine group < 0.25% ropivacaine group < 0.5% ropivacaine group; the onset time of motor block was longest in the 0.25% ropivacaine group, followed by 0.5% ropivacaine group, and shortest in the 0.15% ropivacaine group (all P < 0.05). (2) The order of the visual analogue scale scores at 1, 5, and 10 hours after anesthesia and 24 hours postoperatively was as follows: 0.5% ropivacaine group < 0.25% ropivacaine group < 0.15% ropivacaine group (P < 0.05). (3) The change levels of heart rate and blood pressure were highest in the 0.5% ropivacaine group, followed by 0.15% ropivacaine group, and lowest in the 0.25% ropivacaine group. (4) The incidence of adverse events was highest in the 0.5% ropivacaine group, followed by 0.15% ropivacaine group, and lowest in the 0.25% ropivacaine group (P < 0.05). (5) To conclude, 3 mL of 0.25% ropivacaine exhibits desired anesthetic effect.

Objective To explore the safety of the human bone marrow‐derived mesenchymal stem cells (hBMSCs) after silencing of human leukocyte antigen A2 expression .Methods We divided the cells into three groups:normal cultured cells of the 8th passage served as control group , and hBMSCs after HLA A2 silencing expression of the 5th and 15th passage as experimental groups 1 and 2 ,respectively .The hBMSCs were recultured by sterile methods .The growth curve ,telomerase activation ,and expressions of P27 ,cyclin D2 and cyclin‐dependent kinase 4 (CDK4) were utilized to explore the safety of the hBMSCs induced by LV‐siRNA‐HLA A2 .The BMSCs were transplanted to the subcutaneous layer of nude mice .Tissue types were detected 24 weeks after transplantation . Results The cell curves had no obvious left or right shift in all the groups . The telomerease activation in experimental groups 1 and 2 did not significantly differ from those in control group . The expressions of anti‐oncogene P27 ,cyclin D2 and CDK4 had no obvious difference between the two experimental groups and control group , either . There was only ectopic osteogenesis 24 weeks after the BMSCs (HLA A2 gene silenced ) were transplanted to the subcutaneous layer of the nude mice .Conclusion There was no obvious evidence to support that hBMSCs had undergone change in safety after the silencing of HLA A 2 expression .

Objective To study the relationship between expression of multidrug resistant 1 ( MDR1) gene encoded P-glycoprotein ( P-gp) and multidrug resistance relation protein ( MRP) in ulcerative colitis and the disease behavior. Methods Sections of endoscopic biopsy samples from patients with ulcerative colitis were studied by immunohistochemistry with monoclonal antibody of MDR1 gene encoded P-gp and MRP. Results Expressions of MDR1 gene encoded P-gp and MRP in the 12th month and 18th month of ulcerative colitis were 40. 5% , 45.9% , 48. 6% and 51.4% , respectively, which were all significantly higher than those of early phase ( P < 0.05). P-gp and MRP expressions in active stage and remission stage of ulcerative colitis were 36.4% , 18. 6% , 46. 1% and 25.4% , respectively, which was significantly different between the two stages (P < 0.05 ). At active stage of ulcerative colitis there was no significant difference in expressions of P-gp and MRP among mild, moderate and severe patients (P > 0. 05 ). Conclusion Examination of P-gp and MRP expressions is feasible and dependable in presenting evidence of drug insistence. However, it is not suitable to be an indicator of severity because of its poor differentiation ability in active stage.

Objective To explore the effect of applying problem-based learning (PBL) combined with ' pre-resident physician' teaching model in clinical practice in the nephrology department.Methods Sixty students in clinical practice were divided into two groups:experimental group and control group.The students in experimental group were received PBL in combination with 'pre-resident physician' teaching model while those in control group were received traditional teaching model.The effect was evaluated according to the results of theoretic examination and investigation.The results were analyzed by x2 test and P ＜ 0.05 signified statistically significant.Results The average score of theoretic examination in experimental group was 80.22 while that in control group was 76.53.The students in experimental group had significant higher score than those in control group (P ＜ 0.05 ).The overall satisfaction degree was 93.00％ in experimental group concerning the 8 indicators in PBL combined 'pre-resident physician' teaching model,while that was 73.75 ％ in control group concerning the 8 indicators in traditional teaching mode.Concerning the aspects of teaching flexibility,arousing learning interest,training literature capacity and improving ability of independent problem solving,the satisfaction degree was higher in experimental group than in control group (P ＜ 0.05 ).Conclusion PBL in combination with ' pre-resident physician' teaching model has evident advantages in clinical practice in nephrology department.

ObjectiveTo compare the effect and safety of ropivacaine mesylate with ropivacaine hydrochloride in epidural anesthesia of patients make hypogastric region and lower extremity operation.Methods126 patients with epidural anesthesia were divided into two groups,each group 63 cases.The observation group was administered with ropivacaine masylate,and the control group was administered with ropivacaine hydrochloride.The sense of pain block plane and effective-acting period and effect time,sports block rating and effect-acting period and duration,vital signs,adverse events and serious adverse events were observed.ResultsThere were no significant differences between the two groups on sense of pain block plane and effective-acting period and effect time,sports block rating and effect-acting period and duration,Bp,HR,SpO2,chang of ECG,bleeding in operation( t =13.23,10.52,10.64,12.21,13.23,10.52,10.64,12.21,6.11,5.34,5.23,6.05,all P ＞ 0.05 ).ConclusionThe results of ropivacaine mesylate used for anesthesia epidural was satisfied and had no obvious side effects as well as ropivacaine hydrochloride.