Objective To study the expression of KGF and E-cadherin in pancreatic adenocarcinoma and their clinical significance.Methods The expression of KGF and E-cadherin in 45specimens of paraffin-embeded pancreatic adenocarcinoma and 22 normal pancreas amund tumors was detected by using immunohistochemistry staining(sP)and compared with clinicopathological data.Results The increased expression of KGF in pancreatic adenocarcinoma was significantly higher than that in normal pancreas,the positive rate of KGF was 77.8%(35/45),and there was a relationship between hish KGF expression and the differentiation of carcinoma and the clinical stages(P＜0.05,0.05,respectively).The decreased expression of E-cadherin in pancreatic adenocarcinoma was lower than that in normal pancreas.The higher rate of expression was 35.6%(16/45),and a significant relationship was observed between low Ecadherin expression and the differentiation of carcinoma.the metastasis of lymph nodes and the clinical stages (P＜0.05,0.05,0.01,respectively).The expression between KGF and E-cadherin had a significant relation in pancreatic adenoeareinoma (P＜0.01).Conclusion The level of KGF and E-cadherin may be a sensitive predictor of proliferation,differentiation,invasion and metastasis of pancreatic adenocarcinoma.

The main functions of modern Clinical Engineer(CE) is discussed. By combining experience of clinical engineering in developed country with clinical practice in our country, the work character problem of clinical engineering was proposed. According to the reality of equipment management and operation, the function of CE is not only to ensure medical equipment to run normally but also estimate the value of equipment in before buying or abandoning, PSS and technology study. Through the efforts of CE, the main functions of equipment can be made the best, medical therapy and new method research is more efficient.

Objective To examine the expression change of HIF-1α and VEGF in protein levels and the corelation of them in Q8C939 cell line under chemical hypoxia induced by CoCl2 in vitro. Methods ELISA and immunohistochemical technique were used to examine the change of VEGF and HIF-1α protein level in QBC-939 line in different concentrations (200, 150, 100, 50, 0 μmol/L) of COCl2 and different periods (6 h, 12 h, 24 h, 48 h) in hypoxia. Results CoCl2 can up-regulate the expression of HIF-1α and VEGF at least partly in a dose and time dependent pattern. ELISA demonstrated that the level of VEGF protein in different concentrations and different hypoxia periods, the result showed that the VEGF protein was in different concentration groups over contrast group (P<0.05). The VEGF protein also increased continuously with the growing of hypoxia time (P<0.05). Immunohistochemical detection of concentrations of different groups, HIF-1α, VEGF protein in the average gray compared with the control group was different(P<0.05). Different time periods of hypoxia HIF-1α, VEGF protein in the average gray, any two group comparisons have difference (P<0.05). Different concentrations, the normal control group, different time period HIF-1α and VEGF protein expression of relevance were analyzed. The correlation coefficient was 0.830, P <0.01, 0.909, P <0.01. Conclusion In QBC939 cell line of hypoxia, there is up-regulation HIF-1α and VEGF.HIF-1α regulated the expression of VEGF.

Epithelial-mesenchymal transition is not only involved in generating normal embryonic development of organs and tissues,but also helps to improve cancer cell invasion and metastasis.When epithelial-nesenchymal is stimulated,cell-cell adhesion and cell polarity are disrupted by disappearance of adhesion molecules such as Ecadherin,resulting in the acquisition of a mesenchymal phenotype.Consequently,cells which have acquired mesenchymal function activate the migratory and invasion capability in cancer.Studies have shown that part of the transcription factors,growth factors and signaling pathways involved in epithelial-nesenchymal process,and miRNA may participates in the proliferation,apoptosis,invasion and metastasis of gastric cancer cells by regulating oncogenes or tumor suppressor genes,which also relates to the regulation of epithelial-nesenchymal.This article will be reviewed in recent years specific miRNA regulatory mechanisms of gastric cancer epithelial-nesenchymal.

MicroRNA (miRNA) is an endogenous nonprotein-coding short-chain RNA,and it involves in the evolution of a variety of malignant tumors through targeted inhibition of mRNA translation or mRNA cleavage of gene expression regulation cancer or tumor suppressor genes,meanwhile,is also an important molecule in the occurrence and development processes of tumor.In the recent studies,miRNA has played an important role in the proliferation,apoptosis and invasion of cholangiocarcinoma cells,and it maybe become a new biomarker for the early diagnosis,assessment of prognosis and chemotherapy of cholangiocarcinoma.

Objective To investigate the safety and feasibility of laparoscopic pancreaticoduodenectomy (LPD). Methods Clinical data of 9 patients who underwent LPD operation from September 2014 to February 2017 in the first hospital of Shanxi Medical University were analyzed retrospectively. Results 9 patients attempted LPD, while one required conversation to open procedure. Two cases underwent hand assisted laparoscopic surgery .The average operative time was (553 ± 86.1) minutes, the average amount of bleeding was (333.3 ± 304.1) ml, and the average hospitalization time after operation was (25.3 ± 8.9) d. Postoperative bowel sound recovery time was (3.5 ± 1) d. Postoperative complications included pancreatic leakage in 4 cases (A grade pancreatic leakage in 1 cases, B grade in 3 case), lymph leakage in 1 case, abdominal hemorrhage in 1 case , gastric emptying in 2 cases , and bile leakage in 1 case. All patients with postoperative complications were cured by non operative treatment. Postoperative pathological examination showed that 5 cases had duodenal papillary adenocarcinoma, 1 case had pancreatic ductal carcinoma, 2 cases had common bile duct disruption differentiation of carcinoma, and 1 case had pancreatic head retention cyst. Patients were followed up 3 to 24 months after operation. 1 case of pancreatic duct died 17 months after operation. One case of middle bile duct carcinoma 12 months after surgery had liver and retroperitoneal lymph node metastasis. The remaining 7 cases had no complications. Conclusions laparoscopic pancreaticoduodenectomy is minimally invasive, safe and feasible.

r cirrhosis. NO and ET-1 are involved corporately in the development of liver cirrhosis, but not the main factors facilitating hypoxemia of liver cirrhosis.

Objective To compare the clinical utility of s7 and s670 intracoronary sents in the management of patients with coronary artery disease. Methods The clinical follow-up outcomes of 68 patients after implantation of s7 (n=32; group Ⅰ) and s670 (n=36;group Ⅱ) intracoronary stents were retrospectively analysed. Results The successful procedure rates were 100% in group Ⅰ and 97.2% in groupⅡ. The follow-up period was six months for both groups. In group Ⅰ, there were no death, no re-infarction and no revascularization but 2 patients experienced reccurrence of angina ( 6.25%). In group Ⅱ, there were 1 death, 1 patient undergoing revascularization, 3 patients suffering from reccurrence of angina ( 13.8%). Conclusions Both s7 and s670 intracoronary stents appear to be safe and effective in the treatment of coronary lesions with favorable outcomes.

Objective To study the relationship between sex hormones and uric acid in elderly women with coronary heart disease. Methods One handred and fifteen post menopausal female patients were divided into two groups by angiographic results, CAD group (n=61)and control group(n=76).Their serum concentrations of sex hormones and uric acid and general characteristics were compared. Results Baseline clinical characteristics were similar between the two groups,except patients in the CAD group were more likely to have hypertention and diabetes mellitus than the control group .Serum concentrations of estradiol and progestin and androgen showed little difference between CAD and control group,meanwhile, serum uric acid was higher(345.6?96.5 vs 307.9?85.5, P

Objective To observe the effect of AS1411-mediated signal transduction and activator of transcription 3 (STAT3) antisense oligonucleotide (ASO) targeting tumor cells.Methods RNA was used as coupling molecules to link the targeting molecules AS1411 and effector molecules ASO.Prediction and analysis of the secondary structure of the pre-synthesis of chimeric molecules by RNA Structure software.Agarose gel electrophoresis was used to test the stability of chimeric molecules in serum and cell lysis solution.Using flow cytometry and confocal fluorescence microscope were used to estimate the internalization of AS1411-mediated STAT3 ASO.Inhibitory effect of ASO on the growth of tumor cells was detected by CCK-8 kit.RT-PCR and Western blot was used to measure the expression levels of tumor related genes.Results STAT3 ASOs mediated by AS1411 can enter tumor cells efficiently to inhibit the transcription and translation of C-myc,Cyclin D1,Bcl-xl and PD-L1 gene,and also can inhibit the growth of Du145 cells.Conclusions AS1411-mediated STAT3 ASO can enter tumor cells and act as anti-tumor medicine.