Chinese compound priscriptions of traditional herbs are widely used in the alternative therapy for all kinds of diseases It remains unclear how the compounds in different herbs interact each other and which exacting mechanism can complain their effects The components and pharmacological effects of the combination of Chinese herbs are not due to the simple mixture of single herb's The substance basis research of combination herbs is one of the important issue for understanding their pharmacological mechanism Here the recent progress on the substance basis research of combination Chinese traditional herbs is reviewed, including the combinative effects and quantity ratio relation study, herbs serum study, chemical compounds research, the methods and techniques in chemical components analysis, and application foreground of Chinese compound priscription

A large number of studies have confirmed that antiplatelet agents reduce the relative risk of stroke,myocardial infarction,or death by an average of 22％.However,many patients still have thrombotic events during the period of taking anti-platelet agents,and this is called anti-platelet agent resistance.Its incidences reported are very different.The incidence of aspirin is 3％ to 85％,and that of clopidogrel is 28％ to 44％.The exact cause of antiplatelet agent resistance remains unclear.It may be associated with several factors,including decreased drug bioavailability,genetic polymorphisms,activation of other platelet activation pathways,and increased circulating platelets.Currently,a variety of laboratory methods have been used to detect antiplatelet agent resistance,its criteria are different.In addition,the response measures of antiplatelet agent resistance also have no conclusion,and this has brought greater difficulties for the prevention and treatment of stroke.

Objective To investigate the application of three dimensional blood vessel angiography(3D-CTA) in the patients of spontaneous subarachnoid hemorrhge diagonised as aneurysm.Methods 3D-CTA were examined in 32 cases of spontaneous subarachnoid hemorrhage.Results In 32 patients of spontaneous subarachnoid thirty patients were diagnosed as aneurysm.Two cases were not diagnosed.Conclusion 3D-CTA may be the first examination method in spontaneous subarchnoid hemorrhage.

Objective: To investigate the preventive and theraputic effects of Astragalus and Angelica mixture(A&A)on renal tubulointerstitial fibrosis after unilateral ureteral obstruction(UUO)in rats and their mechanisms. Methods: UUO rats were randomly divided into Sham, UUO, A&A or ACEI groups. A&A, ACEI or the same amount of water was administered by gavage beginning 24 hours before UUO preparation and continued through ten days after UUO. Sera and the kidney tissues were collected from each group on the tenth day. Scr and BUN were measured. Trichrome staining, measurement of tubulo interstitial damage index and immunohistochemical studies localizing ? smooth muscle actin(? SMA), TGF ? 1, fibronectin(FN), laminin(LN)were carried out. Results: In UUO rats, the tubular interstitial damage index, the expressions of ? SMA, TGF ? 1, FN and LN were all increased compared with those of Sham group. The tubulo interstitial damage index had positive correlation with expressions of ? SMA, TGF ? 1, FN and LN. A&A significantly ameliorated deterioration of renal function, tubulo interstitial damage index and inhibited the over expressions of ? SMA, TGF ? 1, FN and LN in UUO rats. These anti fibrotic effects were similar to those affected by ACEI. Conclusion: In renal interstitial fibrosis induced UUO rats, A&A retard the progression of renal fibrosis and renal function deterioration by inhibiting myofibroblasts and suppressing TGF ? 1 expression, which may consequently result in a decreased production of extracellular matrix.

ObjectiveTo study the clinical and pathological characteristics of tubulointerstitial nephropathy associated with Chinese herb patent Guan Xin Su He Wan(GXSHW-TIN).Methods15 patients with GXSHW-TIN were studied.Clinical and pathological data were semi-quantitatively assessed.Relationships between medication and the incidence,clinical characteristics and the outcome of the disease were analyzed.ResultsAll the patients had chronic renal failure when GXSHW-TIN was diagnosed.They all got the disease after long-term taking of GXSHW in routine dosage.Most of the patients presented gastrointestinal dysfunction,abnormal urine analysis and mild to moderate anemia as onset symptoms.The pathological characteristics were similar to those of Guanmutong(Aristolochia manshuriensis Kom) induced chronic aristolochic acid nephropathy(AAN).ConclusionLong-term taking of GXSHW,which contains Radix Aristolochiae,might induce AAN.It indicates that GXSHW should be causious for clinical use,the ban of Radix Aristolochiae in the pharmaceutical market needs to be considered for prevention of AAN.

To observe the clinical effect of Yangxue Zhitong pills combined with Shuangbaisan on the treatment of children with hip synovitis in.Methods 60 children with hip synovitis from November 2013 to May 2015 were randomly divided into the observation group and the control group,30 cases in each group.The observation group were given conventional treatment and the control group was given Yangxuezhitong pills combined with Shuangbaisan.Hip VAS and blood flow index in the two groups were followed-up and compared.Results (5 days after treatment,hip VAS in the observation group(3.50±0.46),was lower than that of the control group(4.51±0.55)(P<0.05).②CRP,TNF-α,IL-6,IL-1 level in the observation group were(3.01±1.73mg/L,15.58±5.46pg/mlL,74.9±19.4pg/mL,22.57±4.01ng/L),which were lower than those in the control group(5.69±2.05mg/L,32.47±4.16pg/mL,97.6±24.2pg/mL,32.17±4.38ng/L)(P<0.05).③After treatment,total effective rate in the observation group(93.3％)was higher than that in the control group 66.7％(P<0.05).Conclusion The clinical therapeutic effect of Yangxue Zhitong pill combined with Shuangbaisan on the treatment of children with hip synovitis is exact,which is worthy of further clinical research and application.

ObjectiveTo evaluate the ability of high resolution magnetic resonance (MR) imaging at 3.0 tesla to depict the characterization of human carotid arterial vessel wall and detect atherosclerotic lesions ex vivo in comparison to histopathologic results. MethodsEighteen carotid arteries obtained from 9 elderly donors underwent fat-suppressed T1 - and T2-weighted MR imaging at 3.0 tesla MR system with a mouse coil. Corresponding histological sections were obtained for the comparison. Correlation between MR images and histopathologic slices was obtained by Pearson or Spearman correlation coefficient.Cohen K was computed to quantify the agreement between MRI and histopathologic findings.Results Lumen area,intima and media area measured on fat-suppressed T2-weighted images showed stronger correlation with the corresponding histopathologic slices [ MRI vs.histopathology:( 27. 53 ± 6. 77 ) mm2 vs.( 25.83 ±6. 69 ) mm2, r2 ＝ 0. 91,P2 ＝ 0. 024, ( 12.31 ± 3. 31 ) mm2 vs.( 12. 28 ± 3.71 ) mm2, r2 ＝ 0. 70, P2 ＝ 0. 020,Median 12. 29 mm2(Min 1.12 mm2, Max 33.18 mm2) vs.Median 11.62 mm2(Min 0.89 mm2, Max 32. 84 mm2 ), r2 ＝ 0. 74, P2 ＝ 0. 016, respectively]. The Cohen K score between the MR imaging and American Heart Association classifications was 0. 74, which corresponds to a good agreement. Conclusions 3.0 T high-resolution multi-sequence MRI can clearly show the structure of ex vivo carotid artery wall and allow quantitative assessment.Fat-suppressed T2Wimaging has a greater advantagein presenting atherosclerotic lesions.

Objective To investigate the effect of heat treatment on the proliferation of, melanin synthesis and tyrosinase activity in cultured normal human melanocytes. Methods Normal human foreskin tissue was obtained by sterile circumcision and melanocytes were harvested by using methods for epidermal cell culture. Basic fibroblast growth factor (bFGF) was utilized as the primary mitogen to establish the culture system of normal human epidermal melanocytes. Masson-Fontana staining was proformed to identify melanocytes.Third-passage melanocytes were treated with hyperthermia at various temperatures (39 ℃, 41 ℃, 42 ℃, 43 ℃ and 45℃) for 1 hour a day for consecutive 3 days followed by the measurement of cell viability with MTT assay. The hyperthermia at optimized temperature was used to treat fourth-passage melanocytes for 1 hour a day for consecutive 3 days; subsequently, the tyrosinase activity were detected with L-Dopa as the substrate, and melanin content was determined in heat-treated and untreated (control) melanocytes. Results The hyperthermia at 42 ℃ exhibited the strongest promotive effect on the proliferation of melanocytes among these 5 hyperthermia conditions. After treatment with hyperthermia at 42 ℃ for 1 hour a day for consecutive 3 days, melanocytes showed an increment in tyrosinase activity by 36.4% and melanin synthesis by 78% compared with the untreated melanocytes (both P＜0.05). Conclusions Heat treatment can enhance the proliferation of cultured human melanocytes, promote their melanin synthesis and tyrosinase activity.

Objective To examine cognitive function of the HIV/AIDS patients in the hospitals measured by the neuropsychological tests battery,make an evaluation of cognitive function of the HIV/AIDS patients,and diagnose the patients with HIV-associated neurocognitive disorders(HAND).Methods Ninety-nine normal individuals and two hundred and thirty HIV/AIDS inpatients,who were sampled by multi-stage random stratified in Guangxi,were tested by the 12 items of the neuropsychological tests.Results HIV / AIDS patients with abnormal neuropsychological test entries proportion ranges located between 21.3％-58.7 ％.The results of 12 items of the neuropsychological tests were associated with the results of clinical diagnosis.Among them,the results of SC time,SCW time,color attachment,and numbers of the contiguous error in WSCT tests were positively associated with the results of clinical diagnosis,and the correlation coefficient were 0.384,0.335,0.237 and 0.129,respectively.There was a negative correlation between the results of the rest assessment and the results of clinical diagnosis ; vocabulary fluency was the highest correlation coefficient,while numbers of the contiguous error in WSCT tests was the lowest.Conclusion A higher proportion of abnormal occurrence of Guangxi HIV / AIDS patients in the 12 neuropsychological test entry,and affect the subjects with or without HIV-related neurological assessment of cognitive impairment.The data suggest that a full neuropsychological tests can be better used for the preliminary study of the HIV ±/AIDS patients neurocognitive function.

Objective To investigate the clinical effect of surgical treatment of acetabular fractures. Methods From June 1995 to December 2000, 62 cases of acetabular fracture with dislocation were treated with open reduction and internal fixation. There were 13 cases with fractures of the posterior wall, two with posterior column fractures, three with anterior column fractures, five with transverse fractures, 15 with transverse and posterior wall fractures, three with posterior column and wall fractures, five with T-shaped fractures, four with anterior and hemi-transverse fractures and 12 with fractures of both column. Kocher-Langenbeck (K-L) approach was applied in 37 cases, ilioinguinal approach in 12, extended iliofemoral approach in four, iliofemoral approach in two, and combined approaches (K-L+ilio-inguinal) in seven. Results Anatomic reduction was done in 37 cases, with satisfactory results in 17 and unsatisfactory results in eight. Reduction for joint vallatae was performed in four cases. The follow-up was 1-5 years (average 2.7 years). The total excellence rate of clinical results was 71% (44/62), with excellence rate in anatomic and non-anatomic reduction groups for 89% (33/37) and 44% (11/25), respectively, with a very significant difference (?2=22.89, P