The purpose of this article is to d iscuss the meaning and the method of testing the ER/PR.We use the anti-ER、 ant i-PR monoclonal antibody SP method to detect the ER/PR in 26 cases of the endome trial cancinoma endometrium、23 cases of the atypical hyperplasia and 22 cases o f the normal endometrium respectively.The result shows that the SP method to t est the ER/PR has different result in carcinoma、atypical hyperplasia and normal endometrium.The expression of ER/PR in carcinoma is distinctly lower than th at in the atypical hyperplasia and normal endometrium.There is no significant d ifferent between the atypical hyperplasia and normal endometrium of the expressi on of ER/PR.However,there are significant different in the ER.PR expressions b e tween normal endometrium and endometrial careinoma,and between atypical hyperpla sia and endometrial careinoma,reflecfing the special biological action of the tu mor.SP method to test the expression of ER/PR has some special meaning to estim ate the prognosis and supervises the treatment of the carcinoma of the uterine.

Objective To detect the specific serum protein of colorectal cancer by surface-enhanced laser desorption/ionization tune of flight mass spectrometry(SELDI-TOF-MS)and provide the foundation for early detection of colorectal cancer.Methods The levels of serum protein of 36 patients with colorectal cancer and 36 healthy control subjects were detected by weak cation exchanger protein chip(WCX2)chip and SELDI-TOF-MS.Results At the M/Z value range from 2 000 Da to 30 000 Da,82 protein peaks were found.The level of 3 604.240 Da protein was significantly higher in patients with colorectal cancer than in healthy control subjects,while the level of 12 861.368 Da protein was significantly higher in healthy control subjects than in patients with colorectal cancer(P＜0.05).The specificity,sensitivity,and positive predictive value of the detection of colorectal cancer using these 2 protien were 86.11%,77.78%,and 87.5%.respectively.Conclusion The 3 604.240 Da and 12 861.368 Da proteins can be used as the serum marker for early detection of colorectal cancer.

ObjectiveTo investigate the value of urinary BNP for diagnosis of chronic heart failure (CHF). MethodsThe levels of Urinary BNP and plasma BNP were measured by microparticle enzyme immunoassay (MEIA) in 83 patients with CHF and 30 control subjects. The heart function was classified according totheNYHAcriteria. Leftventricularejectionfractions(LVEF)weremeasuredby echocardiology. ResultsThe level of urinary BNP in patients with CHF was[90. 0(38. 3 -209. 5 )]ng/L and the level of plasma BNP was[680. 0 ( 289. 7 - 1543.5)]ng/L, both of them were much higher than those in healthy subjects,[17. 0 ( 13.0 - 33. 0)]ng/L and[84. 5 ( 56. 0 - 158.0 )]ng/L, respectively (P＜0. 01 ). The concentrations of urinary BNP increased gradually with more severe symptoms ( NYHA cl ass Ⅰ -ⅣV ). The level of urinary BNP was positively correlated with NYHA class ( r = 0. 742, P ＜ 0. 01 )and the level of plasma BNP (r =0. 842,P ＜0. 01 ) while negatively related with LVEF (r = -0. 801 ,P ＜0. 01 ). The level of urinary BNP in patients with LVEF ＜ 40％ was[143.0 ( 85. 0 - 258.0)]ng/L , which was much higher than that in patients with LVEF≥40％ ,[31.5( 17.3 -38. 8)]ng/L, (P ＜0. 01 ). At a decision threshold of 36. 5 ng/L, the urine BNP assay demonstrated a clinical sensitivity and specificity of 84％ and 80％ ,respectively. In this study,the area under the curve(AUC) was 0. 905. ConclusionUrinary BNP is a new candidate marker for the diagnosis of CHF,it provides a similar accuracy with plasma BNP.

Background and purpose:Runt-related transcription factor 3 (RUNX3) has a signiifcant relation with gastric cancer. Many researches have confirmed that rs760805 AA can increase the risk of gastric cancer. The purpose of the study was to investigate the relationship between the rs760805 polymorphism of the RUNX3 gene and gastric cancer. Methods: The rs760805 genotypes were determined by PCR-based DNA sequence measuring analysis and direct DNA sequencing in 310 incident cases with gastric cancer and 327 controls recruited in Shandong. Results:The frequency of TT genotype was 15.16%in gastric cancer patients and 20.49%in normal controls, and the corresponding percentages for AT and AA genotypes were 48.39%and 36.45%, and 52.60%and 26.91%, respectively. Compared to TT genotype, AT genotypes were associated with an increased risk of gastric cancer (OR=1.24, 95%CI`:0.81-1.92;OR=1.83, 95%CI:1.15-2.92). Conclusion:The rs760805 polymorphism of the RUNX3 gene is associated with increased susceptibility to gastric cancer.

Objective Nano-structured lipid carriers ( NLC) is a concerned research area in pharmaceutics , which as a novel drug delivery system to promote oral absorption of poorly soluble drugs .The literatures were reviewed to explore and summarize the mechanism that nano-structured lipid carriers in promotion of the absorption of poorly soluble drugs .

Objective To review the newly developed solid self-microemulsifying drug delivery system (S-SMEDDS).Meth-ods Relevant literatures at home and abroad in recent years were consulted and summarized .Results Solid self-microemulsions car-rier, solidification technology and controlled release formulations were discussed , in order to provide relevant references for improving the bioavailability of water-insoluble drugs and the SMEDDS technology for drug release characteristics .Conclusion The utilization of the solid self-emulsifying drug delivery system could significantly enhance the oral bioavailability of water -insoluble drugs .As a new formulation, S-SMEDDS presented huge potential .

Since the end of 2019, the COVID-19 caused by 2019-nCoV has emerged and the pandemic ravaged the world, which seriously threatens global public health security and economic development. 2019-nCoV vaccine is an effective weapon to combat the viral infection, however, studies have shown that vaccine-induced immune protection decreases over time, coupled with some novel and immune escape variants continual emerging. Therefore, it is urgent to complete booster immunization to improve protection. At present, 2019-nCoV vaccines based on a variety of technical platforms have been approved and available in China. Therefore, we developed this sequential vaccination strategy guide to provide documentation guidance for the prevention and control of the epidemic caused by 2019-nCoV and its variant strains.

Objective To explore the effects of evidence-based nursing on postoperative nutriture and complications in patients with thoracic cancer.Methods A total of 256 patients with thoracic cancer admitted in the Fifth People's Hospital of Datong from August 2014 to May 2018 were selected by convenient sampling.Totally 126 patients admitted between August 2014 and June 2017 were divided into the control group who received conventional nursing care post surgery,while another 130 patients admitted between July 2017 to May 2018 were divided into the observation group who received evidence-based nursing care.The content of albumin and hemoglobin,pain scores,anxiety and depression scores,and the incidence rate of complications were compared between the two groups before surgery and 1 month after surgery.Results The content of albumin in the observation group 1 month post surgery was higher than that before surgery and that in the control group (P＜0.01).There was no statistical difference in the content of hemoglobin between the two groups before and after surgery (P＞0.05).The Self-Depression Scale (SDS) score and Self-Anxiety Scale (SAS) score was lower in the observation group than in the control group 1 month post surgery (P＜0.01).There was no significant difference in the Visual Analogue Scale (VAS) score between the two groups 24 hours post surgery (P＞0.05);the VAS score in the observation group was lower than that in the control group days 3,7 and 10 post surgery (P＜0.01);and there was significant difference in the VSA score between the two groups 1 month post surgery (P＞0.05).The incidence rate of complications was 8.46% in the observation group,lower than that of the control group (P＜0.05),which was 22.22%.Conclusions Evidence-based nursing can reduce the pain and the incidence rate of complications postoperatively in patients with thoracic cancer,improve the content of albumin in the patients' body,and ameliorate their negative emotions such as anxiety and depression,which is worth promoting in clinical practice.

Objective: To establish the information solution for the classification and assessment of specimen quality based on the assembly line. Methods: Before the samples entered into the assembly line, they were took pictures and screened by the image results. For the suspected samples, serum index was detected. Then, the classification criteria of specimen quality were set, and the alarm thresholds of serum indices for each item suitable for our laboratory were established. The results of serum indices were compiled into the corresponding text descriptions and automatically written into the notes of the result reports. The pictures of blood collection tubes were stored in the laboratory information management system (LIMS) and could be accessed at any time for verification. The samples intercepted by the automatic review were further reviewed by manual. Results: The intra-assay coefficients of variation (CV) of serum indices for haemolysis (H), lipaemia (L) and icterus (I) were 0.6%, 0.7% and 1.3%, respectively, indicating that the precision was good. Among 657 770 samples detected by the assembly line, 11.9% of samples were screened out before they entered the assembly line. The detection of serum indices of these samples demonstrated that the samples with haemolysis, lipaemia and icterus accounted for 1.6%, 1.2% and 0.3% of the total samples, respectively. According to the results of the interference experiment, the alarm threshold of hemolytic serum index was set in 11 items, and those of lipaemia and icterus were set in 1 item. Conclusion By establishing the information solution of specimen quality based on the assembly line, the real-time classification prompting of specimen quality is realized, and the missed detection is avoided, which is helpful to reduce the pre-analysis errors caused by serum quality and simplify the laboratory workflow.

In recent years, both benign and malignant thyroid tumors have grown rapidly in the world, and have become one of the most common tumors in the endocrine system. At present, the pathogenesis of thyroid tumor is still unclear, but more and more studies have found that certain factors are related to the occurrence and development of thyroid tumors. It is of great significance to summarize and analyze these risk factors. This article reviews the research progress of its risk factors reported in recent years, so as to provide a basis for the accuracy and scientific prevention and control of thyroid tumors.