Objective To improve HIFU focal spot accuracy.Methods Combining with actual production,the influencing factors of ultrasound spot accuracy were analyzed from the aspect of mechanical equipment.In order to ensure the first focal accuracy,suitable piezoelectric ceramics flakes were selected by measuring single probe acoustic field.Reasonable designing projection planning was used to ensure the second focal accuracy.Results By means of general positioning of H7h6 clearance fit,focal spot accuracy error of ?66mm probe was kept under 0.05mm.If(?+?)was kept under 0.12?,the focal spot accuracy could be controlled under 0.5mm.Conclusion During the development of HIFU,focal spot accuracy can be improved by taking effective measures to ensure two focal accuracies.[Chinese Medical Equipment Journal,2008,29(2):28-30]

Objective To study the clinical effect of homemade rapamycin-eluting stent on acute myocardial infarction(AMI).Methods A total of 116 AMI patients who received emergency PCI were divided into two groups,the Cypher group(n=52)and the Partner group(n=64).There was no obvious difference in ages,female ratio,smoking,hypertension,hyperlipidema and diabetes between the two groups.The MACE and the restenosis were the targets we wanted to observe.Results The emergency PCI were all successful.Fifty-five Cypher stents and sixty-four Partner stents were planted.The MACE was 0 during in-hospital and the follow-up.Eleven patients in Cypher group(21.15%)and eighteen patients in Partner group(28.13%)underwent coronary angiography re-examination at the 6 months' post-stenting,the restenosis both being 0.Conclusion The homemade rapamycin-eluting stent is safe and effective in AMI.

To evaluate the curative effect of antisense telomerase ODN on malignant glioma cells. The activity of telomerase in 16 fresh malignant gliomas was detected positive by PCR ELISA method. After covered with lipofectin, the 5?mol/L telomerase ODN was incubated with cultured gliomas cells. PCNA, TUNEL positive rate and the phase of cell cycle were detected by FcM per 24h. The results showed: Anti sense ODN inhibited telomerase activity after being applied for 48 hours, and inhibition was complete at 72 hours, The ODN inhibited proliferation of malignant gliomas and promoted their apoptosis markedly at 72 hours. After 96 hours, most transfected glioma cells were stopped to grow at G 2/M phase, and the number of apoptosis cells increased as time went on. The results suggested that anti sense telomerase ODN could inhibit the telomerase activity, and in turn promote aptoptosis of glioma cells and inhibit the growth of the tumor.

To establish the proliferation and apoptosis index(P/AI), and to explore its relationship to the malignant degree of gliomas. Specimens of normal brain tissues obtained from 8 patients undergoing decompression operation for head injury, and 69 paraffin-embedded specimens and 26 fresh specimens of the glioma were studied. Immunohistochemical staining of proliferation cell nuclear antigen (PCNA) and terminal deoxynucleotidyl transferase mediated dUTP DIG nick end labeling(TUNEL) were used to determine the proliferation and apoptosis rates of the glioma. Grading was defined by the ratio between PCNA positive rate and TUNEL positive rate (P/AI). An increase in P/AI denoted an increase in malignancy. It was found that P/AI of normal brain tissue was 0.2-1.5. In 85.1% of grades Ⅰ and Ⅱ gliomas, P/AI was lower than 2.5 and while in 91.7% of grades Ⅲ and Ⅳ gliomas P/AI was 2.5. There were significant differences between normal group, grades Ⅰ and Ⅱ and grades Ⅲ and Ⅳ over gliomas (P

To separate the biological radar echo signals in different breathing modes. Echo signals of bioradar-based vital signs monitoring system were acquired in different breathing modes, and an improved signal separa-tion algorithm was used to obtain respiratory and heartbeat signals as well as their parameters. Under two breath-ing modes, the center frequencies of the signals from the self-adaptive filter were kept consistent with those of heartbeat signals, and the signals with other frequencies were suppressed effectively. The algorithm can be used to sep-arate heartbeat signals while suppress other interference signals effectively. ZHANG Jing and LIU Qian are the first au-thors who contributed equally to the article.

Bioradar is based on the integration of theory of radar and bio-medical engineering,which can detect the life-parameters in farther distance.It is new concept radar presented by foreign experts.The technology can be widely used in detection of lifeform signal and non-contact clinical monitoring.Biomedical signal processing method is the premise that the technology can be realized.The signals can be interfered by the environmental factors,breath motion and so on,especially the influences of breath motion which can not be solved by average methods.A large number of signal processing methods are used in various aspects of the technology.This review introduces the progress of bioradar technology and the application of the current signal processing methods in the field.

Objective To detect circulating CD4 + CD25 + regulatory T cells (Treg) and Toll-like receptor(TLR)2 and TLR4 expression on the peripheral blood mononuclear cells (PBMCs) of patients with HBV-related liver cirrhosis (LC), and to explore the correlation between them. Methods PBMCs isolated from 30 LC patients, 21 chronic hepatitis B (CHB) patients and 16 normal controls(NC) were stained with fluorescent labeling anti-TLR2-PE, anti-TLR4-APC, anti-CD14-FITC monoclonal antibodies and anti-CD4-PerCP, anti-CD25-FITC, anti-CD127-PE. Samples were detected by flow cytometry. Statistic analysis be-tween groups was performed by Kruskal-Wallis H test. Spearman rank correlation was used to analyze the correlation of Treg and TLR2, TLR4. Results The expression of TLR2 and TLR4 were significantly up-reg-ulated in patients with LC than those in the controls (TLR2 : 200.3 ± 96.8 vs 94.1 ± 17.6, P < 0.05 ; TLR4:32.1 ±7.2 vs 17.8 ±3.9, P<0.05). The expression of TLR4 was significantly increased in pa-tients with LC than those in patients with CHB (TLR4 : 32. 1 ± 7.2 vs 25.2 ± 8.3, P < 0.05), but there were no differences of TLR2 expression between LC and CHB(200.3 ± 96.8 vs 214.0 ± 72.6, P > 0.05). Treg/CD4+ T cells were 5.07% ±1.43%, 5.88% ±1.66%, 4.21% ±1.24% in patients with LC, CHB and NC, respectively. Treg/CD4+ T cells were significantly increased in patients with CHB than those in pa-tients with NC(P<0. 05) and LC(P <0.05), but there were no differences between LC and NC(P > 0.05). TLR4 expression and Treg were positive correlation (r = 0. 469, P = 0. 032) and TLB2 expression were negative correlation in patients with LC (r = -0.428, P = 0.021). Conclusion The expression of TLR2 and TLR4 were up-regulated on PBMCs in patients with LC. It seems to be expression of TLR2 and TLR4 in-volved in the pathogenesis of LC.

Objective To research the tourniquet effect on cement mantle thickness in total knee arthroplasty.Methods From June 2013 to June 2014,112 cases of patients were received total knee arthroplasty in the First People's Hospital of Foshan and 94 cases of which received primary operation,82 cases were recruited of the research and randomly divided into experimental group(n=41) without tourniquet and control group(n =41)with tourniquet.The radiological cement mantle thickness was evaluated postoperatively in 2 zones (tibia) on anteroposterior and 4 zones (tibia 2;femur 2) on lateral radiographs,and values were cumulated.Additionally,the calculated blood loss,haemoglobin loss,blood transfusion rate,average transfusion volume,VAS pain score,arc of motion,swelling,ecchymosis and micro thrombus in venules were recorded.Results The study showed that (3.57± 0.62) mm on without tourniquet group and(3.74 ±0.71)mm on tourniquet group in tibia (P=0.240).However,the cement mantle thickness of mm on without tourniquet group(2.00±0.43) mm on tourniquet group(2.19±0.48) in femur (P=0.053),there was no statistically significant difference between two groups.The tourniquet group were reduced on the calculated blood loss,haemoglobin loss,blood transfusion rate and average transfusion volume compared with without tourniquet group(P＜0.05).But VAS pain score,arc of motion,swelling,ecchymosis and micro thrombus in venules were slightly increased in tourniquet group compared with without tourniquet group (P＜0.05).Conclusion The use of a tourniquet in total knee arthroplasty can reduce the calculated blood loss,haemoglobin loss,blood transfusion rate,average transfusion volume,but without using a tourniquet has a better clinical results.

Objective To determine serum concentration of methotrexate in children with acute lymphoblastic leukemia by HPLC and explore the application practice.Methods The separation was performed in a Wondasil C18-WR column (4.6 mm ×150 mm, 5 μm) with a mobile phase of methanol-0.15%phosphoric acid solution(21:79 ) and determined at 306 nm.The sample of serum was centrifuged after protein precipitation with perchloric acid.Detected results for children to establish monitoring files.ResuIts The linearity was well at 0.02 ~50 μg/mL of methotrexate ( r =0.9990,n=6).The average recovery was 96.50%, RSD of within day and between days were less than 15%.Established monitoring files for six children and after 3 courses of treatment, the blood concentration of methotrexate in 4 children were small and they were in a safe range , which could reduce the frequency of monitoring.ConcIusion This method is simple, sensitive and accurate for methotrexate detection in serum.The children with stable concentration and within safety range, can reduce monitioring frequency.It could bring the children less pain and relieve the burden of family.

Objective To investigate effect of glucose and lipid metabolism on acute cerebral infarction patients by Rhodiola injection. Methods 201 cases of acute cerebral infarction patients were selected,and divided into experiment group and control group.The control group of 101 cases were treated by clinical routine therapy,the experiment group 100 cases were treated with Rhodiola injection.The clinical efficacy, blood rheology and blood lipids, blood glucose index were compared before and after treatment.Results After 2 weeks of treatment, the total effective rate in experimental group was significantly higher than the control group(χ2 =12.596, P<0.05), with statistical difference.Patients in the experimental group,FBG was significantly lower than the control group, the difference was statistically significant (t=8.830, P<0.05).Patients in the experimental group TG, TC, LDL-C were significantly lower than that of control group, HDL-C was significantly higher than control group (P<0.05).Blood rheology indexes decreased significantly better than the control group ( P<0.05 ) .Conclusion Rhodiola injection on patients with acute cerebral infarction has good clinical curative effect, and can effectively improve the blood lipid, blood glucose level, reduce blood viscosity, is of great significance to the clinical treatment of cerebral infarction.