Objective To evaluate the efficacy of early intervention of tendon technique combined with physical therapy on patients afterlumbar disc herniation (LDH) surgery in functional recovery. Methods 44 patients after LDH surgery were randomly divided into interventiongroup (n=20) and control group (n=24). They were accessed by Japanese Orthopaedic Association score (JOA score), Oswestry DisabilityIndex (ODI) and electromyogram (EMG) before surgery, 2 weeks and 3 months after surgery. Results The scores of JOA and ODIwere better in intervention group than in the control group (P<0.05) 2 weeks and 3 months after surgery. There was no significant differencebetween 2 groups in latency, amplitude, nerve conduction velocity during the same period (P>0.05). The absolute value added of motornerve conduction velocity 3 months after surgery was higher in the intervention group than in the control group. Conclusion Early interventionof tendon technique combined with physical therapy on patients after LDH surgery has positive effect in functional recovery, and wasuseful in promoting recovery of neurological function.

Objective To evaluate the effect of dezocine on the c-fos expression in neurons in the midbrain periaqueductal gray in a rat model of incisional pain.Methods Thirty-six pathogen-free healthy adult male Wistar rats,weighing 250-300 g,were divided into 3 groups (n =12 each) using a random number table:control group (group C),incisional pain group (group I) and dezocine group (group D).A 1 cm longitudinal incision was made through skin,fascia and muscle of the plantar aspect of the right hind paw in sevoflurane-anesthetized rats.In group C,the rats were only anesthetized and underwent no operation.In group I,0.9％ sodium chloride solution 2 ml was injected via the caudal vein at 15 min before the model was established.In group D,dezocine 1 mg/kg (diluted to 2 ml in 0.9％ sodium chloride solution) was injected via the caudal vein at 15 min before the model was established.At 24 h before operation (T0) and 2,6 and 24 h after operation (T1-3),the mechanical paw withdrawal threshold (MWT) and cumulative pain score were measured.After measurement of the pain threshold at T3,the whole brain was removed for determination of the c-fos expression in neurons in the midbrain periaqueductal gray by immunohistochemistry.Results Compared with group C,the MWT was significantly decreased,cumulative pain scores were increased,and the expression of c-fos in neurons in the midbrain periaqueductal gray was upregulated at T1-3 in I and D groups (P＜0.05).Compared with group I,the MWT was significantly increased,the cumulative pain score was decreased,and the expression of c-fos protein in neurons in the midbrain periaqueductal gray was down-regulated at T1.3 in group D (P＜0.05).Conclusion Dezocine mitigates incisional pain through inhibiting the expression of c-fos in neurons in the midbrain periaqueductal gray of rats.

Objective　To investigate the protective effect of dexmedetomidine (DEX) on apoptosis of hippocampal neurons in rats with status epilepticus (SE) by regulating MAPK/ERK-CREB pathway.Methods　SE rat model was established by intraperitoneal injection of lithium pilocarpine,and was randomly divided into 4 groups,with 10 rats in each group.1 μmol/L of DEX was injected intraperitoneally in the SE+DEX group and 1 μmol/L of phenobarbital was injected intraperitoneally in the positive control group,after 24 hours of drug intervention,Nissl and TUNEL methods were used to detect the damage and apoptosis of hippocampal neurons,and Western blot was used to detect the expression of MAPK,p-ERK,p-CREB and caspase-3,Bcl-2,Bax in hippocampus of rats.Results　Compared with the control group,the Racine scores of SE group,positive control group and SE+DEX group were significantly higher (P<0.05),the number of hippocampal neurons and the expression of Bcl-2 protein expressions were decreased significantly (P<0.05),and the number of sepia TUNEL positive cells,MAPK,p-ERK,p-CREB,caspase-3,Bax,Bax/Bcl-2 protein levels increased significantly (P<0.05).Compared with the SE group,the Racine scores of the positive control group and the SE+DEX group were significantly lower (P<0.05),the number of hippocampal neurons and the expression of Bcl-2 protein were significantly increased (P<0.05),and the number of sepia TUNEL positive cells,MAPK,p-ERK,p-CREB,caspase-3,Bax,Bax/Bcl-2 protein expressions decreased significantly (P<0.05).Conclusions　DEX may inhibit apoptosis of hippocampal neurons by inhibiting MAPK/ERK-CREB pathway,and then improve epilepsy in rats.

Objective:To evaluate the effect of transcutaneous electrical acupoint stimulation (TEAS) on the intracranial pressure in patients undergoing laparoscopic hysterectomy by measuring the optic nerve sheath diameter (ONSD) via ultrasound.Methods:Forty-two American Society of Anesthesiologists Physical Status classification Ⅰ or Ⅱ patients, aged 18-60 yr, with a body mass index of 18-28 kg/m 2, scheduled for elective laparoscopic hysterectomy, were divided into 2 groups ( n=21 each) using a random number table method: control group (group C) and TEAS group (group T). In group T, TEAS was applied to the Yintang (EX-HN3) and Taiyang (EX-HN5) acupoints at 30 min before anesthesia, the stimulation used alternating dense-disperse waves at a frequency of 2/100 Hz, with the current intensity starting at 1 mA and increasing to the maximum tolerable level just below the pain threshold, and stimulation was maintained at the Hegu (LI4), Quanliao (SI18), and Fengchi (GB20) acupoints during surgery until the procedure was completed. Patients had electrode pads applied to the corresponding acupoints without electrical stimulation in group C. The ONSD was measured upon entry into the operating room (T 0), 3 min after anesthesia induction (T 1), 5 min after trendelenburg position (T 2), 30 min after trendelenburg position (T 3), 60 min after trendelenburg position (T 4), and immediately after the end of operation (T 5). Results:Compared to the baseline at T 0, the ONSD was significantly increased at T 3-5 in group C and at T 4, 5 in group T ( P<0.05). The ONSD was significantly lower at T 4, 5 in group T than in group C ( P<0.05). Conclusions:TEAS can reduce the intracranial pressure to some extent in patients undergoing laparoscopic hysterectomy.