OBJECTIVE:To prepare powder of promoting blood circulation to arrest pain and to establish its quality con?trol method.METHODS:Qualitative identification of panax pseudo-ginseng,frankincense and dragon blood in the powder of promoting blood circulation to arrest pain was performed by TLC method.The content of dracorhodin in the dragon blood was determined by TLC Scanning Method.RESULTS:Good linear relationship was achieved when the sample size of dracorhodin perchlorate remained within the scope of0.6?g～3.2?g(r=0.9999).The average recovery rate was98.22%(RSD=1.53%).CONCLUSION:This preparation method is feasible,and the quality control method is simple,accurate and reproducible.

OBJECTIVE:To provide references for the evolving and standardization of Chinese online pharmacy regulation. METHODS: We explored the online pharmacy regulations in the USA and European countries in term of the mode, scope and means of regulation as well as analyzed the existing problem in online pharmacy of China. RESULTS & CONCLUSIONS: Some issues such as administrative regulation and self-discipline of industries, domestic regulation and international cooperation, pharmacy regulation and consumer education etc should be taken into fully consideration in the practice of Chinese online pharmacy regulation.

Objective To investigate the relationship between Alzheimer disease(AD) and plasma homocysteine (HCY) level. Methods Rate of mini mental state examination(MMSE) and levels of plasma homocysteine and folate and vitamin B12 were determined in 68 AD patients, which were compared with 60 healthy older. Results The mean plasma level of HCY was higher in AD patients than that in contrast group,and the very reverse of plasma level of folate and vitamin B12(P15?mol/L,the odd ratio(OR) was 4.3(95% CI 1.89～7.43). Plasma level of HCY was negatively correlated with that of folate and vitamin B12 and rate of MMSE in two groups. Conclusion The high level of plasma HCY is an important risky factor to AD patients. The lack of nutrition elements of folate and vitamin B12 et al can result in the rising of plasma HCY.

OBJECTIVE: To discuss the geographic mark protection of genuine Chinese crude drugs so as to promote its intellectual property protection. METHODS: Zhe-Ba-Wei was taken as an example to reveal the crisis of the development of Genuine Chinese Crude Drugs; the protection means between trade mark and Geographic Mark was compared, and some special problems arose in Geographic Mark Protection of Genuine Chinese Crude Drugs were discussed. RESULTS & CONCLUSION: Geographic mark protection is an effective legal means. However, a series of problems, such as confirmation of best habitat, establishment of Chinese crude drugs standard, and confliction between geographic name and geographic mark etc remained to be tackled.

OBJECTIVE: To introduce the methodology validation and quality control in bioanalytical procedures and its expected trend of development.METHODS: According to the bioanalytical validation reflected in FDA guidelines and other publications,based on the guidance of SFDA and the standard operating procedure of our phase I clinical trial lab,we summarized the procedure and questions of bioanalytical methodology validation.RESULTS: The methodology validation and quality control in bioanalytical procedures and the possible risks involved in the methodology validation were summarized.CONCLUSIONS: The established methodology validation in bioanalytical procedure and quality control contents were basically consummate,but the standards for the methodology validation should be formulated based on reality.

Objective Dynamical observation the development law of rejection of small intestinal transplantation(SIT) in inbred rats BN to F334,to study the value of this SIT madel in the rsesarch of rejection. Methods Heterotopic SIT models were established by microsurgical technique according to modified monchik method in inbred strain F344/RT1 l andBN/RT1 n,including isotransplantation (F344→F344, n =8 ,ITx group) and allotransplantation (BN→F344, n =8, ATx group). Results (1) The mean survival time was over 30 days in AT X group, 12 days in IT X group(P

S-adenosylmethionine(SAM) is a physiologically active molecule in all the organizations and the human body fluids, and it is important to participate in a variety of biochemical reactions, the regulation of liver regeneration, liver cells differentiation and various sensitivity of the injury. The SAM affects the liver through a variety of ways. It is confirmed that the SAM and MTA can block the LPS-induced TNF-α of Kupffer cells to protect the liver. In a word, S-adenosylmethionine may be beneficial to LPS-induced liver in-jury in clinical treatment.

OBJECTIVE: To evaluate the effect of Eucommiae ulmoides on the microstructural and ultrastructural change of femur and lumber vertebrae in ovariectomized rats.METHODS: 80 4-month-old female SD rats were randomized to 4 groups: sham operation group,model group,alfacalcidol group,E.ulmoides group.In the sham operation group,the abdominal cavities of the rats were cut open to expose ovaries before being sutured.However,the rats in the other 3 groups were ovariectomized bilaterally.At 10 days after operation the rats were administered intragastrically with corresponding drug.After 3 months,femur and lumber vertebrae were dissected and fixed in 4% glutaral for 3 days,then decalcified in 4% EDTA for 4 weeks,and then the structure of femur and lumber vertebrae was observed under light microscope and transmission electron microscope(TEM).RESULTS: Observed under light microscope,the femur and lumber vertebrae were full of fat in the ovariectomized rats and in which the density of bone trabecula decreased significantly;however,in alfacalcidol-treated rats and E.ulmoides-treated rats,the amount of fat decreased markedly while the density and thickness of bone trabecula increased.Observed under TEM,in the ovariectomized rats,rough endoplasmic reticulum(RER),cytolysosome and mitochondrial were plentiful inside osteoclasts in femur and lumber vertebrae,partial osteoblasts atrophied,the amount of RER and other cellular organ decreased significantly and apoptosis appeared for partial cells;however,in alfacalcidol-treated group and E.ulmoicdes-treated group,the above changes improved significantly.CONCLUSION:E.ulmoides has remarkable beneficial effect on osteoporosis in ovariectomized rats.The mechanism might be attributed to its inhibition on the synthesis of fat and the activity of osteoclasts,promotion on the activity of osteoblasts and inhibition on the apoptosis.

Cohort study also named follow up study,has been used in clinical trials of traditional Chinese medicine(TCM),such as prognostic evaluation and therapeutic effect evaluation.Therefore follow up is an important step in a cohort study,and follow up quality will directly affect the statistical analysis and drawing a conclusion.This article introduced the aim,the content,the method and duration of follow up,we also discussed the relevant issues when conducting a follow up and the methods to deel with problems.We hope this article can be helpful to researchers of TCM.

Objective To set up an efficient and rapid method for detecting CK20 expression in peripheral blood of patients with colorectal carcinoma, and explore the relationship between CK20 expression and micrometastasis of colorectal carcinoma. Methods CK20 mRNA expression in peripheral blood of 36 patients with colorectal carcinomas was detected at various time points before and after operation by fluorescent RT-PCR. The mutation of p53 gene was also detected using SSCP. Results The positive rate of CK20 mRNA expression in peripheral blood before and 24 hours after operation was 75%(27/36) and 83.3%(30/36), respectively. After the short-term chemotherapy, the positive rate of CK20 mRNA expression was 36%(13/36). No mutation of p53 gene was found in those patients. Conclusion Fluorescent RT-PCR for detecting CK20 mRNA expression in peripheral blood was simple and efficacious. It was useful to monitor micrometastasis of colorectal carcinoma.