Objective To determine the content of calycosin-7-?-O-D-glucopyranpside in Danggui Buxie Dropping Pills.Methods HPLC method was used. The chromatographic column was YMC-C18(150?4.6 mm, 5 ?m), the mobile phase was methanol and water(40 ∶60), the detection wavelenth was 235 nm, column temperature at 20 ℃and flow rate was 1.0mL/min.Results Calycosin-7-?-O-D-glucopyranpside was separated well with other components and was in good linearity in the range of 0.02～0.80 ?g. The average recovery was 97.62 %and RSD=1.06 %.Conclusion The method is rapid and accurate and can be used for quality control of Danggui Buxie Dropping Pills.

The new educational system integrating both medical undergraduate and professional degrees,by integrating the curriculum system rationally,strengthening the clinical practice,innovating the assessment and management mode,establishes and enforces normative resident physician training system to cultivate high-starting point general practitioners led by medical colleges and provide qualified general practitioners,for the purpose of supporting the health reform successfully.

The incidence of thyroid carcinoma is associated with a variety of factors.Radiation is the clear risk factor,the relationship between iodine intake and thyroid carcinoma remains controversial.Researches show that the genetic and epigenetic changes of many signaling pathways are the key of molecular pathogenetic mechanism of thyroid carcinoma.In addition,thyroid stimulating hormone,body mass index and chronic lymphocytic thyroiditis are also associated with thyroid carcinoma.

New antibiotics with novel modes of action and structures are urgently needed to combat the emergence of multidrug-resistant bacteria. Bacterial signal peptidase I (SPase I) is an indispensable enzyme responsible for cleaving the signal peptide of preprotein to release the matured proteins. Increasing evidence suggests that SPase I plays a crucial role in bacterial pathogenesis by regulating the excretion of a variety of virulent factors, maturation of quorum sensing factor and the intrinsic resistance against beta-lactams. Recently, breakthrough has been achieved in the understanding of three-dimensional structure of SPase I as well as the mechanism of enzyme-inhibitors interaction. Three families of inhibitors are identified, i.e. signal peptide derivatives, beta-lactams and arylomycins. In this article, we summarize the recent advance in the study of structure, activity and structure-activity relationship of SPase I inhibitors.

Objective This article is to explore the application of hospital in the new pattern of elderly health care service.Methods It takes 115 elderly who come from elderly health center as respondents , using questionnaire and inter-view, to study their satisfaction with the new pattern of elderly health care service , i.e.the combination of nursing , support and treatment , their living quality , happiness index and monthly average hospitalization expenses before their going to a nurs -ery and after.Results Their satisfactions are greatly increased after going to a nursery .Their living quality and happiness index are also higher when they go to a nursery (P<0.05, P<0.01), and the hospitalization expenses are lower after their going to a nursery (P<0.01).Conclusion Applying hospital in the new pattern of elderly health care service , elderly′s satisfactions with the new pattern of elderly health care service have been greatly increased , so have their living quality and happiness index .Meanwhile , the hospitalization expenses are also greatly decreased .The scope of the community has a cer-tain influence , and it is worth promoting .

Objective To analyze the related factors,and discuss the causes and prevention measures of pharyngocutaneous fistula caused by total laryngectomy.Methods The complete chnical data of 118 patients underwent laryngeal ancer surgery were analyzed retrospectively.Analyzed the relationship between pharyngocutaneous fistula and the factors of sex,age,pre-operative nutrition states,preoperative radiotherapy,prior tracheotomy,neck dissection,tumor position,clinical stage of tumor,patho-staging,post-operative infection comobidity with analysis of single factor.Results Among the 118 cases of laryngeal carcinoma,17 cases were pharyngocutaneous fistula after operation,the incidence was 14.4％ (17/118).According to the statistical analysis results,the predominant influencing factors in the causation of pharyngocutaneous fistula were:pre-operative nutrition states,pre-operative radiotherapy,clinical stage of tumor and post-operative infection.Concusion Pharyngocutaneous fistula after total laryngectomy is related to many factors,avoiding the above mentioned factors can reduce the rate of fistula and improve the life quality.

Objective To compare the efficacies between nasal packing and no nasal packing after endoscopic sinus surgery for chronic sinusitis,and explore the feasibility of no nasal packing after endoscopic sinus surgery.Methods Eighty-two type 1 or type 2 chronic sinusitis patients who received endoscopic sinus surgery under general anesthesia were divided into no nasal packing group (44 cases,75 sides) and nasal packing group (38 cases,65 sides) by random digits table method.The patients in 2 groups were taken preoperative anti-inflammatory and regulation blood pressure treatment.The volume of bleeding,postoperative pain (visual analogue score,VAS),degree of mucosal edema,efficacy and postoperative complications between 2 groups were compared.Results There were no statistical difference in volume of bleeding and efficacy between 2 groups (P ＞ 0.05).The postoperative VAS in no nasal packing group was significantly lower than that in nasal packing group [(2.46 ± 0.54) scores vs.(5.49 ± 1.26) scores],there was statistical difference (P＜ 0.01).There was no statistical difference in mucosal edema score postoperative 1 week and 6 months between 2 groups (P ＞ 0.05),but the mucosal edema score postoperative 1 month in no nasal packing group was significantly lower than that in nasal packing group [(1.32 ±0.63) scores vs.(3.52 ±0.72) scores],there was statistical difference (P ＜ 0.01).Postoperative comphcations in nasal packing group were significantly more than those in no packing group.Conclusion No nasal packing can be performed for type 1 or type 2 chronic sinusitis patients who has no significant bleeding after endoscopic sinus surgery under general anesthesia,it is actually beneficial of relieving distress of the patient and reducing postoperative complications.

Objective To study the effects and clinical significance of tea pigment on 24 hour urinary endothelin(UET) excretion in patients with early stage diabetic nephropathy.Methods 65 cases of early stage diabetic nephropathy were randomly divided into expermental group and control group.Control group was treated by routine treatment and the expermental group was treated by tea pigment,in addition to routine treatment,and was orally given tea pigment capsule 0 24g three time daily,for 8 weeks.The amounts of 24 hours UET and urinary albumin excretion rate(UAER)in the two groups patients before and after treatment were measured using radioimmunoassay method.Results The amounts of 24 hour UET in patients with diabetic nephropathy were elevated significantly as compared with those in normal control group(P0 05).After 8 week's treatment,the amounts of 24 hour UET and UAER in expermental group were significantly lower than those of control group(P

Objective To explore the practice and efficacy of multidisciplinary collaborative management in the prevention and control of multidrug-resistant organism(MDRO)healthcare-associated infection(HAI).Methods MDROs isolated from clinical specimens and MDRO infection in hospitalized patients in April 2012-March 2013(before intervention)and April 2013-March 2014(after intervention)were analyzed retrospectively.Since April 2013,multidisciplinary collaborative management has been implemented,isolation of MDROs and MDRO HAI in hospitalized patients before and after intervention were compared and analyzed.Results 798 and 833 MDRO strains were isolated before and after the intervention respectively,isolation rate of MDROs after intervention was lower than that before intervention(25.71%vs 31.89%,P<0.001).After the implementation of multidisciplinary collaborative management,incidence of MDRO HAI decreased from 0.94%before intervention to 0.63%;hand hygiene compliance rate of health care workers(HCWs)increased from 36.44%before intervention to 53.51%;compliance rate of contact isolation increased from 65.29%before intervention to 90.88%;rational usage rate of antimicrobial agents increased from 64.93%before intervention to 72.53%;specimen detection rate in patients with therapeutic antimicrobial use increased from 41.36%before intervention to 58.72%,differences were all statistically significant(all P<0.001).Conclusion The practice of multidisciplinary collaborative management can effectively implement the prevention and control measures of MDRO HAI,effectively reduce the occurrence of MDRO HAI.

Objective To establish a method for data quality control of clinical research data based on electronic data capture (EDC) system.Methods After analyzed the specific characteristics of EDC's workflow process,and also referred to international data management guideline,we established the data quality control strategy by summarizing actual data quality control experience of data management department in Peking University Clinical Research Institute.Results Application of EDC has changed traditional clinical research process,therefore EDC data quality control strategy should be built according to its own process flow,namely,selecting the appropriate EDC system,reasonable design and build electronic case report form (eCRF),standardization research center / user management processes,timely and accurate on line eCRF filling up,accurately capturing/docking ex ternal electronic data,planned data monitor and auditing,timely and efficient query management,strictly data locking and unlocking operation process,and strict electronic record life cycle monitoring.Conclusions At present time,clinical research in China is still in the earlier stage of the transition from traditional paper-based data collection (PDC) to EDC.Establishing and applying particular quality control strategy will optimize the process and results of data quality control.