It has aroused extensive concern that how to check for new or after-maintenance medical instruments and keep unqualified instruments out of clinical fields.By analyzing the present situation of medical equipment checking and quality management,main problems are pointed out.The importance of medical measuring and testing is emphasized.It is suggested medical metrology management should be strengthened and the checking procedures should be improved.

Case teaching is one of the important means to improve teaching effect in the process of pharmacology teaching. According to the teaching object, teaching objectives and requirements to build one useful teaching case library about pharmacology, which can be used and shared for a long time, is the premise of case teaching in pharmacology. According to the actual situation of case teach-ing in our university, this study researched and explored the background of case library building, the basic principles of case compilation, the establishment of team, the teacher-led and the student-oriented processes of case building, and the process of sorting and summarizing cases in the case library build-ing, which promotes the reform in the pharmacology case teaching, improves teaching quality, and also provides a reference for other universities or schools and disciplines on teaching case library building.

Objective To evaluate the effects of low molecular weight heparin(LMWH)and heparin-binding epidermal growth factor(HB-EGF)on the biological function of human trophoblast in first trimester.Methods From Feb.2011 to Nov.2011,the trophoblast isolated from human first trimester chorionic villi was cultured in vitro.Based on variation of LMWH concentration,the trophoblast was classified into 0.025 U/ml group,0.25 U/ml group,2.5 U/ml group,25 U/ml group and 250 U/ml group.In the mean time,based on treatment of heparin,the trophoblast was classified into LMWH group (0.25 U/ml),HB-EGF group(10 μg/L),combination group(LMWH at 0.25 U/ml + HB-EGF at 10 μg/L)and add with DMEM as control group.Cell prolferation was assessed by the methyl thiazolyl tetrazolium(MTY)test,which was showed with the mean absorbance as A value.Cell invasion was measured by transwell,which counted the number of cells migrated to the superficies inferia of filter membrane.Cell differentiation was assessed by the concentration of hCG secretion.Results Compared with control group,the trophoblast proliferation and invasion treated by LMWH at 0.025 U/ml did not show significant difference (P ＞ 0.05).When treated by LWMH at 0.25 U/ml and 2.5 U/ml,trophoblast proliferation and invasion was increased significantly(P ＜ 0.05).When LMWH at 25 U/ml and 250 U/ml,it could inhibit trophoblast proliferation and invasion(P ＜ 0.05).When compared with A value of 0.44 ± 0.04 in control group,the increased A value were 0.51 ± 0.05 in LMWH group,0.56 ± 0.04 in HB-EGF group and 0.69 ± 0.06 in combination group(P ＜ 0.05).In the transwell test,the cell number were 511 ± 78 in LMWH group,669 ± 67 in HB-EGF group and 872±64 in combination group,which were significantly higher than 405 ± 67 in control group(P ＜ 0.05),respectively.And the hCG concentration were(7143 ± 649)U/L in LMWH group,(11 762 ± 1059)U/L in HB-EGF group and(11 015 ± 1084)U/L in combination group,which showed statistical difference with(8182 ± 666)U/L in control group(P ＜ 0.05).Conclusion LMWH could modulate trophoblast proliferation,invasion,and differentiation.HB-EGF is one of important factors involved in effects of LMWH on trophoblast function.

Acute pancreatitis (AP) is activated trypsin-induced pancreatic and its peripheral tissue inflammation caused by their own digestion.The activation in advance of trypsinogen and the inflammation cascade in pancreatic acinar cells are thought to be a key mechanism of the onset and development of AP.Autophagy pathway acting as Ⅱ type of programmed cell death occurs in the early pathological course of AP,blockade of which contributes to aggravating necrosis of acinar cells in AP.This article mainly discussed the recent advances in the understanding of autophagy researches and its function in the mechanism of AP.

Objective To study the relationship between Bcl-2/Bax protein expression and the degree of malignancy of astrocytoma.Methods Bcl-2/Bax protein expression positive rate and apoptotic cell rate were determined using immunohistochemical technique and TUNEL, in 10 normal human brain tissues and 80 human astrocytoma specimens of differently malignant degrees confirmed by pathology separatively. Results Bcl-2/Bax protein positive expression was not found in normal human brain tissues, and apoptotic cell rate was only (0 6?0 1)%. The differences of Bcl-2/Bax protein expression positive rate and apoptotic cell rate were statistically significant in the differently malignant degrees(P

ObjectiveTo investigate the relationship between life meaning and life satisfaction among university students.MethodsTotally 473 university students were investigated with The Purpose In Life Test and Life Satisfaction Scale.Results ( 1 ) The scores of life satisfaction and positive affection in highcr levels of life meaning were significantly higher than those in the lower levels of life meaning( (4.53 ± 1.04)vs(3.38 ± 0.98 ),(2.89 ±0.88)vs(2.04 ± 1.07) ) among the university students(F=27.806,P＜0.01 ; F=13.635,P＜ 0.01 ),however the higher levels of life meaning were significantly lower than the lower levels of life meaning ( ( 1.09 ± 1.12 ) vs ( 2.45 ± 1.41 ) ) in negative affection (F =21.513,P ＜ 0.01 ).( 2 ) The students' life satisfaction was negatively correlated with escape( r=-0.336,P＜0.01 ).The others factors of life meaning were positively correlated with life satisfaction ( r =0.285 ～ 0.380,P ＜ 0.01 ).The negative affection was negatively correlated with life meaning( r =-0.121 ～ -0.335,P ＜ 0.05 ).(3)The escape had significantly negative prediction on life satisfaction and positive affection ( β =-0.133 ～ -0.179,P ＜ 0.05 ).The life of goals and the life of passion had significantly positive prediction on life satisfaction and positive affection ( β =0.170 ～ 0.206,P ＜ 0.05 ).Conclusion Life meaning of the university students is closely related to life satisfaction,the higher levels of life meaning can upgrade individual life satisfaction.

International communication capabilities of traditional Chinese medicine professionals are closely related to the publicity of Chinese medical culture and know-how.Based on the interview results as well as literature research,this paper discusses the qualification of traditional Chinese medicine professionals and proposes five essential elements constituting their international communication capabilities:knowledge and skills of Chinese medicine,foreign language proficiency,knowledge and skills of international communication,a global perspective as well as personal traits.It is expected that it may contribute to the syllabus design,in-service training and talents cultivation in Chinese medical colleges and universities.

BACKGROUND: Low intensity pulsed ultrasound is safe, non-invasive, and has strong penetration. In recent years, more and more studies have indicated that low intensity pulsed ultrasound has important role in promoting the repair of damaged articular cartilage.
OBJECTIVE: To investigate the effect of low intensity pulsed ultrasound on the expression of type II col agen and matrix metal oproteinase 13 in articular cartilage of rabbits of knee arthritis.
METHODS: A total of 20 New Zealand white rabbits were included in this study to establish knee arthritis model. Al models were randomly divided into control group and experimental group, 10 rats in each group. In the experimental group, the right knee joint received low intensity pulsed ultrasound treatment with an ultrasonic fracture healing instrument. In the control group, right knee joint received false low intensity pulsed ultrasound therapy. At 8 weeks after intervention, cartilage tissue was col ected from medial end of tibia and medial end of femur. After conventional treatment, toluidine blue staining was performed. Pathological observation was conducted under a microscope. Quantitative real-time polymerase chain reaction was used to detect matrix metal oproteinase 13 and type II col agen expression in chondrocytes. The general situation of the experimental animals was observed. The results of articular cartilage and the quantitative real-time polymerase chain reaction results of matrix metal oproteinase-13 and type II col agen were observed under a microscope.
RESULTS AND CONCLUSION: (1) Toluidine blue staining and pathological observation: 8 weeks after treatment, obvious fissure appeared in articular cartilages, and extending from the surface to the deep layer in the control group, and there was a lack of staining. In the experimental group, cracks formed on articular cartilage surface, and there was a moderate staining missing. (2) Quantitative real-time polymerase chain reaction: at 5 and 10 days after culture, significant differences in gene expression of matrix metal oproteinase-13 and type II col agen were detected at various time points in the experimental and control groups (P < 0.05). (3) The results showed that the low intensity pulsed ultrasound could affect the gene expression of matrix metal oproteinase-13 and type II col agen in the rabbit model of knee arthritis.

The article reviews the current status of elder abuse from the perspective of epidemiology, characteristics of abuse, risk factors and nursing assessment and management, presenting the idea that nurses are playing a major role in the prevention,detection and resolution of elder abuse. It also introduces that nurse can screen out the situation of elder abuse timely by applying the proper assessment tool flexible and respond to the occurrence of elder abuse as well as avoid the elder abuse again by comprehensive application of nursing knowledge and management skills. Some advices about future research and mismanagement in elder abuse being given.

BACKGROUND:Different local anesthetic solutions for tumescent liposuction are necessary and have a certain effect on the biological characteristics of human adipose stem cels.
OBJECTIVE:To explore the effect of different tumescent local anesthesia on the proliferation and differentiation of human adipose stem cels culturedin vitro.
METHODS: Passage 3 human adipose stem cels were intervened by different tumescent local anesthesia (bupivacaine, ropivacaine, lidocaine). Cels cultured in low-glucose DMEM served as controls. Proliferation ability, lactate dehydrogenase activity in the supernatant and percentage of cels under adipogenic differentiation were detected.
RESULTS AND CONCLUSION:After intervention for 1, 2, 3, 4, 5 days, the supernatant lactate dehydrogenase activity in the bupivacaine, ropivacaine, lidocaine groups was significantly higher than that in the control group (P < 0.05), and moreover, the lactate dehydrogenase activity in the bupivacaine group was significantly higher than that in the ropivacaine group and lidocaine group (P < 0.05). The absorbance value of human adipose stem cels was lower in the bupivacaine, ropivacaine, lidocaine groups than the control group (P < 0.05) as wel as lower in the bupivacaine group than the ropivacaine and lidocaine groups (P < 0.05). There was no significant difference in the percentage of cels under adipogenic differentiation between groups (P > 0.05). Overal, these findings indicate that different tumescent local anesthesia (bupivacaine, ropivacaine, lidocaine) can al inhibit the proliferation of human adipose stem cels, but have no certain effects on the adipogenic differentiation.