It has aroused extensive concern that how to check for new or after-maintenance medical instruments and keep unqualified instruments out of clinical fields.By analyzing the present situation of medical equipment checking and quality management,main problems are pointed out.The importance of medical measuring and testing is emphasized.It is suggested medical metrology management should be strengthened and the checking procedures should be improved.

Case teaching is one of the important means to improve teaching effect in the process of pharmacology teaching. According to the teaching object, teaching objectives and requirements to build one useful teaching case library about pharmacology, which can be used and shared for a long time, is the premise of case teaching in pharmacology. According to the actual situation of case teach-ing in our university, this study researched and explored the background of case library building, the basic principles of case compilation, the establishment of team, the teacher-led and the student-oriented processes of case building, and the process of sorting and summarizing cases in the case library build-ing, which promotes the reform in the pharmacology case teaching, improves teaching quality, and also provides a reference for other universities or schools and disciplines on teaching case library building.

Objective To evaluate the effects of low molecular weight heparin(LMWH)and heparin-binding epidermal growth factor(HB-EGF)on the biological function of human trophoblast in first trimester.Methods From Feb.2011 to Nov.2011,the trophoblast isolated from human first trimester chorionic villi was cultured in vitro.Based on variation of LMWH concentration,the trophoblast was classified into 0.025 U/ml group,0.25 U/ml group,2.5 U/ml group,25 U/ml group and 250 U/ml group.In the mean time,based on treatment of heparin,the trophoblast was classified into LMWH group (0.25 U/ml),HB-EGF group(10 μg/L),combination group(LMWH at 0.25 U/ml + HB-EGF at 10 μg/L)and add with DMEM as control group.Cell prolferation was assessed by the methyl thiazolyl tetrazolium(MTY)test,which was showed with the mean absorbance as A value.Cell invasion was measured by transwell,which counted the number of cells migrated to the superficies inferia of filter membrane.Cell differentiation was assessed by the concentration of hCG secretion.Results Compared with control group,the trophoblast proliferation and invasion treated by LMWH at 0.025 U/ml did not show significant difference (P ＞ 0.05).When treated by LWMH at 0.25 U/ml and 2.5 U/ml,trophoblast proliferation and invasion was increased significantly(P ＜ 0.05).When LMWH at 25 U/ml and 250 U/ml,it could inhibit trophoblast proliferation and invasion(P ＜ 0.05).When compared with A value of 0.44 ± 0.04 in control group,the increased A value were 0.51 ± 0.05 in LMWH group,0.56 ± 0.04 in HB-EGF group and 0.69 ± 0.06 in combination group(P ＜ 0.05).In the transwell test,the cell number were 511 ± 78 in LMWH group,669 ± 67 in HB-EGF group and 872±64 in combination group,which were significantly higher than 405 ± 67 in control group(P ＜ 0.05),respectively.And the hCG concentration were(7143 ± 649)U/L in LMWH group,(11 762 ± 1059)U/L in HB-EGF group and(11 015 ± 1084)U/L in combination group,which showed statistical difference with(8182 ± 666)U/L in control group(P ＜ 0.05).Conclusion LMWH could modulate trophoblast proliferation,invasion,and differentiation.HB-EGF is one of important factors involved in effects of LMWH on trophoblast function.

Objective To study the relationship between Bcl-2/Bax protein expression and the degree of malignancy of astrocytoma.Methods Bcl-2/Bax protein expression positive rate and apoptotic cell rate were determined using immunohistochemical technique and TUNEL, in 10 normal human brain tissues and 80 human astrocytoma specimens of differently malignant degrees confirmed by pathology separatively. Results Bcl-2/Bax protein positive expression was not found in normal human brain tissues, and apoptotic cell rate was only (0 6?0 1)%. The differences of Bcl-2/Bax protein expression positive rate and apoptotic cell rate were statistically significant in the differently malignant degrees(P

Acute pancreatitis (AP) is activated trypsin-induced pancreatic and its peripheral tissue inflammation caused by their own digestion.The activation in advance of trypsinogen and the inflammation cascade in pancreatic acinar cells are thought to be a key mechanism of the onset and development of AP.Autophagy pathway acting as Ⅱ type of programmed cell death occurs in the early pathological course of AP,blockade of which contributes to aggravating necrosis of acinar cells in AP.This article mainly discussed the recent advances in the understanding of autophagy researches and its function in the mechanism of AP.

ObjectiveTo investigate the relationship between life meaning and life satisfaction among university students.MethodsTotally 473 university students were investigated with The Purpose In Life Test and Life Satisfaction Scale.Results ( 1 ) The scores of life satisfaction and positive affection in highcr levels of life meaning were significantly higher than those in the lower levels of life meaning( (4.53 ± 1.04)vs(3.38 ± 0.98 ),(2.89 ±0.88)vs(2.04 ± 1.07) ) among the university students(F=27.806,P＜0.01 ; F=13.635,P＜ 0.01 ),however the higher levels of life meaning were significantly lower than the lower levels of life meaning ( ( 1.09 ± 1.12 ) vs ( 2.45 ± 1.41 ) ) in negative affection (F =21.513,P ＜ 0.01 ).( 2 ) The students' life satisfaction was negatively correlated with escape( r=-0.336,P＜0.01 ).The others factors of life meaning were positively correlated with life satisfaction ( r =0.285 ～ 0.380,P ＜ 0.01 ).The negative affection was negatively correlated with life meaning( r =-0.121 ～ -0.335,P ＜ 0.05 ).(3)The escape had significantly negative prediction on life satisfaction and positive affection ( β =-0.133 ～ -0.179,P ＜ 0.05 ).The life of goals and the life of passion had significantly positive prediction on life satisfaction and positive affection ( β =0.170 ～ 0.206,P ＜ 0.05 ).Conclusion Life meaning of the university students is closely related to life satisfaction,the higher levels of life meaning can upgrade individual life satisfaction.

International communication capabilities of traditional Chinese medicine professionals are closely related to the publicity of Chinese medical culture and know-how.Based on the interview results as well as literature research,this paper discusses the qualification of traditional Chinese medicine professionals and proposes five essential elements constituting their international communication capabilities:knowledge and skills of Chinese medicine,foreign language proficiency,knowledge and skills of international communication,a global perspective as well as personal traits.It is expected that it may contribute to the syllabus design,in-service training and talents cultivation in Chinese medical colleges and universities.

Objective To evaluate changes of venous hemodynamics during laparoscopic cholecystectomy(LC).Methods A total of 25 cases of LC was included in the study.A Doppler ultrasonography of the lower limb veins was performed in all the patients to exclude those with abnormal veins or thrombosis.During the LC,the diameter and venous flow volume of the femoral vein were measured under Doppler ultrasonography.Measurements were obtained at three different times:after the induction of anesthesia but prior to the creation of the pneumoperitoneum,during the pneumoperitoneum,and after abdominal deflation but prior to the reversal of anesthesia.Results All the operations were successfully completed.The mean operation time was 45 min(range,30～70 min).No intraoperative hemorrhage or bile duct complications occurred.No conversion to open surgery was required.All the patients were dismissed from hospital on the first postoperative day.After the establishment of the pneumoperitoneum,the cross-sectional area of the femoral vein was increased from 0.94?0.18 cm2 to 1.12?0.23 cm2(q=3.919,P0.05).The venous flow was significantly increased from 7.62?0.72 cm3/s to 9.65?0.63 cm3/s(q=12.971,P

OBJECTIVE To analyze the pathogenic distribution and antimicrobial resistance in respiratory ward and provide the rational information to use antibiotics reasonably. METHODS All pathogens isolated from patients in a respiratory ward from 2005 to 2007 and drug susceptibility results were retrospectively analyzed. RESULTS Totally 264 strains of pathogenic bacteria were isolated,in which 68 strains of Gram-positive bacteria,165 strains of Gram-negative bacteria and 31 strains of fungi.MRSA prevalence was 77.1% and showed a trend of increase.No vancomycin-resistant Staphylococcus aureus or Enterococcus was detected.The resistance rate of Streptoccocus pneumoniae to penicillin,erythromycin and levofloxacin was 44.4-66.7%.Enterobacter and Acinetobacter baumannii showed stable susceptibility to imipenem.Pseudomonas aeruginosa strains were relatively susceptible to cefoperazone /sulbactam,amikacin,gentamicin,piperacillin/tazobactam,ceftazidine,cefepime,cefoperazone and imipenem. CONCLUSIONS The changes in pathogens and antibiotic resistance in the respiratory ward are consistent with the surveillance data in this country,Gram-negative bacteria are still the most common pathogens and the serious degree of bacterial drug resistance is increasing.Our data are useful for the guidance of rational use of antibiotics.

Seventy days after inoculation of 1. 0 ? 104 M. leprae to the foot--pads,the mice were treated with home-made ofloxacin 150mg/kg daily by gavage 5 times weekly for 63 days. The results showed that M. leprae multiplied well in the foot--pads of untreated mice,but no growth of M. lepare were found in the foot-pads of the mice treated with home-made ofloxacin, imported ofloxacin or rifampin until 12 months after infection of M. leprae. Eleven newly diagnosed and untreated BL/LL patients and 2 recently relapsed patients after dapsone monotherapy were treated with home--made ofloxacin 400mg daily 6 times weekly for 12 weeks. The results showed that the skin lesions of all patients markedly improved at the end of therapy. The mean morphological index(MI) in skin smear decreased from 11. 89% before therapy to 2. 59% at the end of 2 week treatment,and to 0% at the end of 4 week treatment. The mean bacteriological index(MI) decreased from 4. 28 before therapy to 3. 90 at the end of therapy. During the therapy, 4 patients developed type Ⅱ lepra reaction, but all patients tolerated the drug well. The study showed that the therapeutic effect of home-made ofloxacin is similar to that of imported one as reported in the literature. The home-made ofloxacin can be used in multidrug therapy for leprosy.