The present study attempts to investigate the influence of the immune response upon the immunoreactive ?-endorphine (ir-?-EP) levels in anterior lobe (AL) and neurointermediate lobe (NIL) of pituitary and hypothalamus after immunizaion of male adult Wistar rats with sheep erythrocytes by a single intraperitoneal injection. The results showed that the decrease of ir-?-EP concentration in AL tended to be concomitant with the direct plaqueforming cells (direct PFC) level increased. In the 3rd day after immunization, when a few PFC began to appear, the concentration of ir-?-EP in AL decreased significantly (P

OBJECTIVE:To survey the current situation and the trend of the use of the herb injection.METHODS:In?formation concerning the consumption amount and manufacturer of herb injection used in21hospitals of Hangzhou in2002～2003was investigated.The use of herb injection was statistically analyzed by means of sequencing the consumption amount.RESULTS:The consumption amount of the herb injection increased annually;Kanglaite,Shenmai injection ranked in the first place in consumption amount,then those for skeleton disease,cardiovascular and cerebrovascular diseases and hepatic diseases such as Xingnaojing,Gegensu,Ciwujia,Danshen injection.CONCLUSION:There is a great potential for the development of herb injection and the consumption both in money and quantity of herb injection will increase rapidly.

Objective To explore the detection meaning of D-dimer(D-D) and fibrinogen(Fg) in patients with coronary disease.Methods 150 patients with coronary disease were randomly divided into UA group,SA group and AMI group,the D-D was detected by ELASA double resistance to clip method and Fg was detected by CLIUSE methods.Results The D-D in SA group,UA group and AMI group showed significant difference between two groups (SA:UA,t =9.340;SA:AMI,t =18.334; UA:AMI,t =10.240.all P ＜0.01 ).The Fg in SA group,UA group and AMI group showed significant difference between each two groups ( SA:UA,t =11.376; SA:AMI,t =11.730; UA:AMI,t =3.121.all P ＜0.01 ).D-D between one coronary artery disease and two coronaries artery disease,three coronary arteries disease showed significant difference( t =7.017,15.603,all P ＜ 0.01 ) ; and between two coronaries artery disease and three coronary arteries disease showed significant difference,too ( t =8.662,P ＜ 0.01 ).The Fg between one coronary artery disease and two coronaries artery disease,three coronary arteries disease,showed significant difference( t =8.687,11.855,all P ＜ 0.01 ) ; and between two coronaries artery disease and three coronary arteries disease showed significant difference,too( t =4.379,P ＜ 0.0 1 ).Conclusion The D-D and Fg showed obvious difference between different types of coronary disease,and the levels increase along with the disease degrees of coronaries

BACKGROUND:Depending on different pH values, nanohydroxyapatite materials have different purities, whether root canal sealers formed by which exert effects on antimicrobial resistance, root canal closure and apical histocompatibility has no reports.
OBJECTIVE:To compare the antimicrobial and sealant properties of nanohydroxyapatite materials with different pH, nanohydroxyapatite-composite and traditional root canal sealants.
METHODS:We detected the antimicrobial action of nanohydroxyapatite with pH 8, 9, 10, Vitapex and AH-Plus root canal sealants with and without addition of ornidazole against three microbial strains namely Enterococcus faecalis, Candida albicans and Staphylococcus aureus using agar diffusion method. And we also analyzed the endodontic microleakage of six root canal sealants by determining the apical reservoir glucose concentration using Glucose Oxidase Method.
RESULTS AND CONCLUSION:Pure nanohydroxyapatite with different pH did not show antimicrobial properties. Addition of ornidazole to nanohydroxyapatite showed greater inhibitory action against Enterococcus faecalis, lesser in Staphylococcus aureus, fol owed by Candida albicans. Vitapex root canal sealer had inhibitory effects only against Staphylococcus aureus. AH-Plus, itself, had antimicrobial activity against al the three strains, but the antimicrobial activity decreased after addition of ornidazole. Nanohydroxyapatite, as a root canal sealant, was superior to zinc oxide eugenol and Vitapex, but inferior to AH-Plus. Addition of ornidazole to nanohydroxyapatite for a short period showed no impact on sealant properties of the material.

Objective To promote worldwide standardization of methods to determine creatinine by tracing to reference measurement for achieving universal accuracy measurement of creatinine.Methods The results of external laboratory assessment (EQA) survey in China and CAP were compared and analyzed. Results There was significant difference among different measurement methods and assay systems.Conclusion It should promote worldwide standardization of determine creatinine, which makes it trace to reference measurement liquid chromatography-isotope dilution mass spectrometry (LC-IDMS).

OBJECTIVE To analyze the cleaning efficiency of using Ruhof multi-enzyme detergent to clean endoscopies. METHODS Take 80 sets of used endoscopies as samples and clean them with multi-enzyme detergent in "four-trough" way as instructed by the Ministry of Health.After cleaning,the changes in bio-burden residual on the endoscope′s surface and jet obstruction were observed. RESULTS After cleaning the endoscopes with Ruhof multi-enzyme detergent,the bio-burden residual on the endoscope′s surface as well as jet obstruction,and the surface cleanness evaluation value had decreased enormously.The difference value before and after cleaning was significant(P

Objectives: To study the correlation between the dynamic expression of aquaporin-4 (AQP-4) around hematoma and the blood-brain barrier permeability after intracerebral hemorrhage in rats. Methods: Forty-two Sprague-Dawley rats were randomly allocated into sham-operation group, and groups of 6 hours, 12 hours, 1, 3, 7 and 14 days (n=6 in each group) after intracerebral hemorrhage. The intracerebral hemorrhage model in rats was established by injecting autologous arterial blood into caudate nucleus. Immunohistochemical method and Evans blue method were used respectively to detect the dynamic changes of AQP-4 around hematoma and the blood-brain barrier permeability in different time periods after intracerebral hemorrhage in rats. Results: (1) The expression of AQP-4 around hematoma increased gradually 12 hours after intracerebral hemorrhage, it increased significantly at day 1 and reached a maximum at day 3. It was slightly higher than normal level at day 7 and almost returned to normal at day 14 (P

Objective To investigate the mean values of carotid intima-media thickness (IMT) and change features according to gender,segments and age groups in general Chinese population,and provide the basis for the health assessment of carotid ultrasound.Methods GE LOGIQ 7 ultrasound scanner was used to measure IMT of 743 healthy cases aged from 38 to 70 years old.Six segments(bilateral common carotid artery,bilateral carotid bulb,bilateral internal carotid artery) were measured for each case.All patients were divided into 6 age groups across 5 years to be one group.The mean values for each group was calculated.Results The average age of the three sections of carotid IMT gradually increasing linearly related; minimal change in men and women,the magnitude of the age of the internal carotid artery IMT;sinus IMT were greater than the common carotid artery IMT and carotid artery IMT thinnest.the mean carotid IMT of Men and women in the same age group,compared to the 46-50 group,the group of 51-55,56-60 group,the differences were significant (P ＜ 0.05);men and women 41-45 group and 61-65 difference between the groups was not statistically significant (P ＞ 0.05);mean IMT difference of the male groups compared with the older group of women was not statistically significant (P ＞ 0.05).Conclusion Ultrasound scanner can be used to measure IMT accurately.In the 46-60 group,carotid IMT thickening in men than women about 5 years earlier.The mean IMTs for different sex,age groups and segments would greatly benefit vascular health evaluation.It has a great significance in the prevention of cardiovascular and cerebrovascular diseases.

This paper reviewed and analyzed the whole 29 years' compiling and revising histo-ry of Neurology the 5-year programming textbook for national undergraduates. The textbook has been becoming a high-quality textbook with novelty, practicability, readability and systematicness, conform-ing to the need of national education reform and reflecting the characteristics of neurology. Through the review of textbook history, we know that material construction can deepen the educational reform, improve the teaching level and discipline development, promote quality education and personnel train-ing.

Objective To observe the effect of bone marrow mononuclear cells (BMMNCs) transplantation on high mobility group box1 (HMGB1) migration in stroke mouse. Methods BMMNCs were harvest and injected intravenously into stroke mouse model; modified neurological severity scores(mNSS) were tested; brain infarct vol-ume and related protein expression levels were measured, then HMGB1 migration were observed. Results mNSS score and brain infarct volume in transplantation group weresignificantly lower than vehicle group , while HMGB1 expression were significantly higher than vehicle group. The expression levels of RAGE and TNF-α in transplanta-tion group were significantly lower than vehicle group. Immunofluorescence assay showed that the release of HMGB1 in brain penumbra area of transplanted mouse was significantly less than vehicle groups. Conclusions BMMNCs transplantation could inhibit the release of HMGB1 in mouse brain.