Objective To construct recombinant baculovirus expression vector containing hepatitis C virus(HCV) truncated core gene and EGFP gene,and to study the antigenicity of the fusion protein expressed in Sf 9 cells.Methods PCR-amplified HCV truncated core gene and EGFP gene were cloned into the transposed vector pFastBac1 to construct a recombinant plasmid pFastCt-EGFP,by which E.coli DH10Bac was transformed to get the recombinant BacmidCt-EGFP.Insect Sf 9 cells were transfected with BacmidCt-EGFP and the expression of fusion protein Ct-EGFP was screened by SDS-PAGE and Western blotting.Results SDS-PAGE and Western blot analysis showed that fusion protein Ct-EGFP was expressed with the molecular mass of 40 kD.ELISA results showed that the fusion protein reacted with 15 of 28(54%) anti-HCV positive sera.Conclusion The fusion protein Ct-EGFP was expressed in insect Sf9 cells and showed partial antigenicity.

Hilar cholangiocarcinoma (HCCA) or Klatskin tumor is a scirrhous adenocarcinoma that arises from the conjunction of bile duct and hepatic ducts.Hepatic magnetic resonance imaging (MRI) and magnetic resonance cholangiopancreatography (MRCP) are important methods for the diagnosis of HCCA.In this article,the clinical data of 42 patients in the Changhai Hospital and 18 patients in the Eastern Hepatobiliary Surgery Hospital who received MRI and MRCP examination from October 2010 to October 2012 were retrospectively analyzed.According to the pathological features,HCCA could be divided into mass-forming type,infiltrating type and intraductal type.Based on the results of imaging examination and intra-and postoperative pathological examination,Bismuth-Corlette types were decided pre-and postoperatively.The coincidence rate of pre-and postoperative Bismuth-Corlette types was 89.7％ (35/39).Infiltration of liver parenchyma was both detected pre-and postoperatively in 19 patients (16 patients were detected by MRI preoperatively),and the sensitivity of MRI was 84.2％ ; vascular invasion was both detected pre-and postoperatively in 10 patients (12 patients were detected by MRI preoperatively),and the sensitivity of MRI was 83.3％ ; hilar and retroperitoneal lymph node metastasis was both detected pre-and postoperatively in 6 patients (4 patients were detected by MRI preoperatively),and the sensitivity of MRI was 66.7％.Combined application of different sequences and techniques of MRI is helpful in the diagnosis,staging and treatment of HCCA.

Iliac vein compression syndrome is one of the most common lower extremity venous backflow obstruction disorders,which often causes lower limb swelling,venous stasis dermatitis,lower extremity deep venous thrombosis,lower extremity venous valvular insufficiency,etc.At present the commonly used imaging diagnostic means include ultrasonography,multi-slice computed tomography,magnetic resonance and digital subtraction angiography.This paper aims to review the recent progresses in the imaging diagnosis of iliac vein compression syndrome.

Objective　To evaluate the in vitro effect of che motherapeutant on hepatic carcinoma.　Methods　The inhibitory effect of 5-Fu, DDP and 4-PA on Bel-7402 cel ls proliferation was studied by MTT colorimetry.　Resu lts　(1) Bel-7402 was on an active proliferation by the treat ment of 5-Fu and DDP for half an hour, and the proliferation inhibited after 12 hours; but the proliferation was inhibited by 4-PA after half an hour of tr eatment. (2) The inhibitory rates increase with time and were over 50% after 48 ～72 hours of treatment. (3) There was temporarily active proliferation of Bel- 7402 cells in the early period of chemotherapy by 5-Fu and DDP. 　Conclusion　Continuous chemotherapy is effectiv e for the inhibition of Bel-7402 cells proliferation.

Objective To evaluate the percentage of agreement between the ultrasonographic data and the surgical findings,and investigate the characteristics of the ultrasonographic imaging of proliferative diabetic retinopathy.Methods B-scan ultrasonography was performed in 48 patients (62 eyes ) affected by proliferative diabetic retinopathy,before vitreoretinal surgery,by observing and recording the position,shape,range,to evaluate the percentage of agreement between the ultrasonographic data and surgical findings.Results Ultrasonography can show kinds of vitreoretinal changes clearly,and is not affected by the opacity of refractive media.The agreement was quite good for the pathological changes of proliferative diabetic retinopathy.The accurate rates of diagnosis of vitreous hemorrhage,posterior vitreous detachment,retinal detachment,vitreal membranes,vitreoretinal adhesions and preretinal membranes were 100%, 91.9%, 94.9%, 96.4%, 80.0% and 87.9%,respectively.Conclusions B-scan ultrasonography is valuable in proliferative diabetic retinopathy and favorable in the ophthalmic clinic practice.

Seventy days after inoculation of 1. 0 ? 104 M. leprae to the foot--pads,the mice were treated with home-made ofloxacin 150mg/kg daily by gavage 5 times weekly for 63 days. The results showed that M. leprae multiplied well in the foot--pads of untreated mice,but no growth of M. lepare were found in the foot-pads of the mice treated with home-made ofloxacin, imported ofloxacin or rifampin until 12 months after infection of M. leprae. Eleven newly diagnosed and untreated BL/LL patients and 2 recently relapsed patients after dapsone monotherapy were treated with home--made ofloxacin 400mg daily 6 times weekly for 12 weeks. The results showed that the skin lesions of all patients markedly improved at the end of therapy. The mean morphological index(MI) in skin smear decreased from 11. 89% before therapy to 2. 59% at the end of 2 week treatment,and to 0% at the end of 4 week treatment. The mean bacteriological index(MI) decreased from 4. 28 before therapy to 3. 90 at the end of therapy. During the therapy, 4 patients developed type Ⅱ lepra reaction, but all patients tolerated the drug well. The study showed that the therapeutic effect of home-made ofloxacin is similar to that of imported one as reported in the literature. The home-made ofloxacin can be used in multidrug therapy for leprosy.

Objective To evaluate the effectiveness of Leprosy Elimination Compaigns (LEC) integrated with routine case- finding of leprosy in the high leprosy- epidemic areas in Yunnan, Guizhou and Sichuan Provinces. Methods Extensive health education of leprosy in the community, training of paramedical workers on leprosy, clue survey and contact survey were carried out to make diagnosis and provide treatment. Results A total of 122 new leprosy cases were detected in 6 high leprosy- epidemic counties through LEC in 1999 with a detection rate of 3.7/100 000, but in contrast, the annual number of newly detected leprosy cases was 67 cases with an average detection rate of 2/100 000 during the period of 3 years before LEC. Among the newly detected leprosy cases, about 47％ were leprosy household contacts. Conclusion LEC combined with routine activities of case finding can improve the detection of the back- log cases in the communities. In the high epidemic areas, especially in the remote and difficult- to- access areas, majority of leprosy cases can be detected through combining active and passive case- finding methods, providing extensive health education, improving leprosy services in skin clinic, leprosy contact and clue surveys.

Objective To study epidemiological and clinical patterns of leprosy in children in China. Methods The data of leprosy in children from 1989 to 1998 in China were analyzed in terms of gender, infection source, detection mode, skin lesion, nerve damage, leprosy reaction, clinical type and skin smear. Results A total of 22 437 leprosy cases were detected during 1989～ 1998, of which 912 (4.1％ ) were child cases (560 males and 352 females). Average delay in detection was 1.13 years (n=909). The main source of infection was close contact within families, accounting for 69.2％ , and majority of cases were detected through active modes. Among children with leprosy, 96.8％ were with skin lesions, 76.0％ with nerve damage, 4.7％ with leprosy reactions, 12.4％ with grade II disability and 41.2％ with positive skin smears. The average proportion of child cases was 4.2％ in Yunnan, Guizhou and Sichuan Provinces, and 1.3％ in Shandong, Jiangsu and Zhejiang Provinces. The proportion of child cases was stable in Guizhou and Sichuan Provinces during this period, whereas in 1998 it significantly increased to 11.6％ in Yunnan. Conclusion The present study suggests that proportion of children with leprosy is one of the sensitive indicators of leprosy endemicity. The sources of leprosy infection in children is predominately due to the contact with active cases within families.

Objective To study clinical characteristics and the trends of leprosy relapse cases after dapsone(DDS) monotherapy or multidrug therapy(MDT) from 1949 to 1998. Methods The data of leprosy relapse cases in China from 1949 to 1998 were analyzed. Results There were a total of 12 403 leprosy relapse cases during 1949～ 1998 in China with a cumulative relapse rate of 3.28％ . Among them, 11 803 were relapsed after DDS monotherapy with a relapse rate of 3.83％ and 236 were after MDT with a relapse rate of 0.57％ . The relapse rate (0.84％ ) in PB cases which were previously treated with DDS monotherapy a half year was significantly higher than that (0.36％ ) in PB cases treated with MDT a half year. The relapse rate (0.37％ ) in MB cases which were previously treated with DDS monotherapy plus MDT also significantly higher than that (0.23％ ) in MB cases which were just treated with MDT two years. The proportions of cases with gradeⅡ disabilities and positive skin smears in relapse cases were 49.9％ and 69.3％ , respectively. Conclusion The peak of leprosy relapses after DDS monotherapy occurred during 1959～ 1988. The relapses after MDT started at the end of 1980s and may reach its peak in the next decades.

Objective To explore the therapeutic effect of percutaneous coronary intervention in patients with coronary artery disease over 75 years old. Methods Patients underwent percutaneous coronary interventions (PCI) during the period from April 2001 to April 2004 were divided into the elder group (age ≥75 years old) and the younger group (age ≤60 years old). The basic characteristics, immediate success rate and the incidence of major adverse cardiac events (MACE) such as re-angioplasty, myocardial infarction and cardiac death during follow-up were analyzed between the two groups. Results The elder group had enrolled 81 cases (64 male/17 female), while the younger group had enrolled 196 cases (176 male/20 female). History of hypertension, cardiac dysfunction, pulmonary disease, kidney dysfunction and cerebral vascular disease was more commonly found in the elder group. Compared with the younger group, the elder patients were more likely to have multi-vessels lesions (46.9% vs 15.3%, P