A zinc chloride and ethionine resistant mutant 0 5Eth400 5 was o btained from its parent strain Saccharomyces cerevisiae 346 by UV and 60 Co ? ray tr e atment and rational screening The glutathione productivity of the mutant reach e d 165 96mg/L by flask culture, which was 350% higher than the parent strain, an d the glutathione content in the dried cells reached 19 76mg/g, which was 318 6% higher than the parent strain A desecend of only 10 7% in the glutathione yie ld of the mutant was observed after ten times of subculture Therefore, the obtai ned mutant is stable strain that is worthwhile to be studied further

Objective To study the risk factors of nosocomial infections in patients with cerebrovascular disease and prevent and cure them.Methods A retrospective survey on the hospital infeation in patients with cerebrovascular disease in our hospital during March 2003 to March 2005 was couducted.Results The result showed that 61 of the 360 patients had hospital infection,the rate of infection was 16.94%,and the hospital average infection rate in all was 3.92%,hospital infection aging,treatment days in hospital,consciousness invasive operation Hospital infection was a retated.The main infection sites were at respirate tract,the most common bacteria were Gram-Negative bacilli.Conclusions There is higher hospital infection rate among,the acute cerebrvascular patients.Age,the critical patients,invading operations are prmincipal factors.We should prevent it in time.

Objective To explore the efficacy of tentorium cerebelli hiatus incision for treatment of severe brain injury complicated with tentorial herniation. Methods Tentorium cerebelli hiatus incision was performed in 38 patients of severe brain injury with secondary brain stem damage resulted from tentorial herniation after intracranial hematoma evacuation and bone flap craniotomy decompression. The changes of four sides cistern, ambient cistern and interpeduncular cistern before and after operation were observed. Results There was a siginificant difference in the appearance ratio of four sides cistern, ambient cistern and interpeduncular cistern between the preoperation and postoperation(P

Objective To evaluate clinical and procedural characteristics and outcomes of patients with ST-elevation myocardial infarction(STEMI) caused by drug-eluting stent(DES) thrombosis undergoing primary percutaneous coronary intervention(PCI).Methods From October 2004 to June 2007,31 patients with STEMI due to definite DES thrombosis who received primary PCI were retrospectively studied(ST group).Baseline clinical,angiographic,PCI procedural features and 1-year outcomes were recorded.Another consecutive 93 patients with STEMI due to de novo coronary thrombosis were served as control group.The primary endpoint was cumulative rate of major adverse cardiac events(MACE),including death,reoccurrence of myocardial infarction and target vessel revascularization(TVR),during hospitalization and at 1-year follow-up.Results Compared with the control group,patients in ST group were older(69.9?11.4 y and 63.7?13.6 y,P=0.01) and higher comorbidity rates of diabetes(41.9% and 22.6%,P=0.04)and previous history of myocardial infarction(29.0% and 11.8%,P=0.02).The occurrence of TIMI 3 flow immediately after primary PCI was reduced(45.2% and 92.5%,P

Objective:To explore the effect of application in patients undergoing general anesthesia prone surgery on basis of observation of ocular complications and focus on preventive care.Methods:One hundred patients who underwent general anesthesia prone surgery before the start of the ocular complications focused preventive care exploration activity (January to May 2019) were set as the control group. After the ocular complications focused preventive care exploration activity was launched (June to October, 2019) 100 cases of general anesthesia prone surgery in our hospital were set as the experimental group, and the observation indexes after the two groups were compared.Results:The satisfaction scores of nurses and patients in the observation group were (9.40 ± 0.57) and (9.30 ± 0.56) respectively, while those in the control group were (7.51 ± 0.88) and (7.09 ± 1.10) respectively, the difference between the two groups was statistically significant ( t values were 18.013 and 13.063, all P < 0.05). The incidence rates of conjunctival congestion, edema and exposure keratitis in the observation group were 4.00% (4/100), 2.00% (2/100) and 1.00% (1/100), respectively, while those in the control group were 12.00% (12/100), 10.00% (10/100) and 9.00% (9/100), respectively. the difference between the two groups was statistically significant ( χ 2 values were 4.348, 5.674, 6.737, all P< 0.05). Conclusion:The focus on preventive care of ocular complications in patients undergoing general anesthesia prone surgery can significantly reduce the risk of ocular complications in this type of patients, and it is well recognized by both patients and patients.

Aim To investigate the effects of co-administration of Astragalus Saponin Ⅰ(ASI)and bendazac lysine(BDL)on hypertrophy of cultured rat mesangial cells and its mechanism.Methods The levels of collagen Ⅳ and laminin,the percentages of cells in S phase,the relative quantity of transforming growth factor ?1(TGF-?1) mRNA and indexes of oxidative status were assayed after the cells were incubated in different agents for 36 h.Results The percentage of S phase cells in high glucose group(HG) was greatly decreased while those of vitamin E group(VE) and co-administration groups were increased.The relative quantity of TGF-?1 mRNA and the collagen Ⅳ level in co-administration groups were significantly decreased,and the levels of total anti-oxidative capability(T-AOC),activity of catalase(CAT),GSH-PX,and SOD were greatly increased.Furthermore,the significant differences were found between low ASI(AL)group,low BDL(BL) group and co-administration of low ASI and low BDL(AL+BL) group for TGF-?1 mRNA,T-AOC and GSH-PX;the high ASI group(AH),high BDL group(BH) and co-administration of high ASI and high BDL group(AH+BH) for TGF-?1 mRNA and collagen Ⅳ,respectively. Conclusion Co-administration of ASI and BDL has synergetic effects on regulating TGF-?1,collagen Ⅳ,and radical oxidative stress,therefore,is beneficial to protecting rat mesangial cells against hypertrophy.

In order to evaluate the effect of mitofusin-2 gene (mfn2) on proliferation and chemotherapy sensitivity of human breast carcinoma cell line MCF-7 in vitro, pEGFPmfn2 plasmid carrying full length of mitofusin-2 gene was transfected, by using sofast, into MCF-7 cells. Mitofusin-2 gene expression in MCF-7 cells transfected by sofast after 48 h was detected by PCR and Western blotting, and the stable expression of GFP protein in MCF-7 cells by Western blot analysis. The proliferation of MCF-7 cells was assayed by MTT and cell counting. By using PI method, the effects of mfn2 on the cell cycle distribution of MCF-7 were measured. Annexin-V/PI double labeling method was employed to detect the changes in apoptosis induced by chemotherapeutics before and after transfection. The results showed that the MCF-7 cells transfected with mfn2 gene could stably and highly express GFP protein. MTT assay revealed that after transfection of mfn2 cDNA, the proliferation of MCF-7 cells was significantly inhibited. DNA histogram showed that cells arrested in S phase, and the percentage of S phase cells was 42.7, 17.2 and 19.6 in mfn2 cDNA transfection group, blank plasmid transfection group and blank control group, respectively (P<0.05). The apoptosis ratio of the cells transfected with mfn2 gene was increased from 3.56% to 15.95%, that of the cells treated with camptothecin (CAMP) followed by mfn2 gene transfection was 69.6%, and that in blank plasmid transfection group and blank control group was 31.0% and 23.4% respectively (P<0.05). It was suggested that transfection of mfn2 gene could significantly inhibit the proliferation of MCF-7 cells and promote their sensitivity to CAMP with a synergic effect.
Antineoplastic Agents, Phytogenic/pharmacology ; Apoptosis ; Camptothecin/pharmacology ; Cell Cycle ; Cell Line, Tumor ; Cell Proliferation ; Drug Screening Assays, Antitumor ; Flow Cytometry ; Gene Expression Regulation, Neoplastic ; Green Fluorescent Proteins/metabolism ; Membrane Proteins/*biosynthesis ; Membrane Proteins/*genetics ; Mitochondrial Proteins/*biosynthesis ; Mitochondrial Proteins/*genetics ; Transfection

Objective:To evaluate the diagnostic accuracy of 64-channel multislice spiral computed tomography(MSCT) in subjects with presentations suggestive of stable angina or acute coronary syndrome. Methods: Subjects received both 64-channel MSCT and coronary angiography, from Feb. 2006 to Feb. 2007, were enrolled for retrospective analyses. Results of the quantitative coronary angiography were used as the "Golden criteria", accuracy of 64-channel MSCT were evaluated in the overall sample, as well as in subjects suspected of stable angina and acute coronary syndrome. Results: A total of 120 subjects were enrolled in the analyses. On the patient level, the sensitivity, specificity, and accuracy of 64-channel MSCT in detecting significant stenoses were 92.5%, 50%, and 87.5%, respectively. The overall ROC area under curve was 0.71. On the artery level, sensitivity, specificity, positive predictive value, negative predictive value, and ROC area under curve of 64-channel MSCT were 69.9%, 83.8%, 81.1%, 73.7%, and 0.77, respectively. Further analyses showed the sensitivity, specificity, positive predictive value, negative predictive value, and ROC area under curve of 64-channel MSCT in subjects presenting as stable angina and in subjects presenting as acute coronary syndrome were as follows: 70.2% vs 69.2%, 76.2% vs 86.0%, 74.7% vs 85.6%, 71.9% vs 69.9%, and 0.73 vs 0.78. Conclusion: The accuracy rates of 64-chanel MSCT in subjects presenting as stable angina and in subjects presenting as acute coronary syndrome were similar.

In physiological property and growing environment aspects, there are some similarities between Acidthiobacillus ferrooxidans(ATF) and magnetotactic bacteria. ATF shows magnetotacxis under the microscope. The magnetosome can be extracted from ATF by using the extracting method of magnetosome from magnetotactic bacteria. The membrane of ATF was crushed by ultrasonic wave and the magnetosomes in the ATF which mainly contains Fe through chemical detection were attracted by using magnetite. After purifing with sucrose density gradient centrifugation and washing by PBS, the magnetosomes can be seen clearly through a transmission electron microscope. The results indicate there are a small amount of intracellular magnetosomes which made the ATF be magnetotactic under the applied magnetic field. It is the first time to find that ATF was magnetotactic. The magnetotaxis of ATF can be utilized and isolated by the magnetotactic properties to gain the high-performance ATF strains of mineral leaching, which have high activity and different magnetism.