Plasma fibronectin (PFn) was determined serially by both simple radial immunodiffusion (RID) and improved indirect hemoagglutination test (using gelatin-coated sheep RBC) in the serum of 30 patients with severe viral hepatitis (SVH),100 normal subjects,and 30 patients with chronic active hepatitis (CAH).Liver function,serum C3 and immunoglobulin (Ig) were determined simultaneously.Endotoxin was assayed by Limulus lysate test in 21 patients with SVH.The normal value of PFn was 284.31?45.56?g/ml and 1:30.64 (geometrical mean).The PFn level significantly decreased in patients with SVH.Significant correlations were found between PFn levels and the values of total serum bilirubin,prothrombin time,C3 and IgM.Low PFn levels were remarkably associated with the occurrence of endotoxeamia.Serial determinations of PFn in serum can reflect the severity of the illness and prognosis in the patients with SVH.

The key point to define the psychological problem in children is the grasp of degree,also,the key point to educate children is the grasp of degree.Are there some principles to clarify the degree? How to quantify the degree? This paper brought forward the gold zone method of psychological guidance in children and adolescents and defined its quantitive expression as golden section.The gold zone method can grasp the scale set according to the goal,form the proper consumption value,control the interpersonal intimacy,cultivate fine thinking and behavioral pattern,and give appropriate guidance to many basic problems about children's growth.

In 21st century, clinical medicine moves toward a model of individualized health care. The development of the individualized model is based on the research findings of human genomics project including genotype and single nucleotide polymorphisms (SNPs), as well as the new health care model, that is, biological-psychological-social-environmental-spiritual. It reflects the idea of patient-centered care, and corresponds to the traditional practice of Chinese medicine, which addressed the individualized therapy on the basis of symptom pattern differentiation. However, the era of evidence-based medicine affords new meanings of individualized health care and promotes the research on the practice. The practice based on evidence would happen upon the integration of external research evidence, the expertise and experience of practitioners, and the value and preference of patients, so the decision of health care will be conscientious, explicit and judicious. This assay describes the meanings of individualized health care in different medical contexts, the characteristics of individualized practice in Chinese medicine, and proposals for further research in practice.

Cohort study is graded as level II in evidence grading system of evidence-based medicine, secondary to randomized controlled trial. It is one of the important evidence for the evaluation of health care interventions, and it has been applied recently in the evaluation of traditional medicine. The author of the article introduces the basic concept, principle, design, and implementation as well as relevant methodological issues when applied in traditional Chinese medicine.

The quality of clinical trials in traditional Chinese medicine (TCM) was analyzed with respect to their methodology and outcome assessment according to the concepts of evidence-based medicine (EBM). It was suggested that the essential methodological principles of EBM should be applied in clinical trials of TCM, including randomization, control and blindness. The sample size estimation, optimal parameter selection for outcome assessment, compliance and intention-to-treat analysis are all important aspects of randomized controlled trials. To bring the benefits of EBM into clinical trials will improve the quality of research in TCM and also promote international co-operation and communications. It is necessary to establish an assessment system of clinical outcome for TCM on the basis of EBM in order to evaluate the efficacy and safety of TCM objectively and scientifically.

Evidence-based medicine (EBM) has rapidly developed in recent years and is currently a hotspot in international medicine. The guiding principle behind EBM is that any medical decision should be determined based on objective clinical and scientific evidence, doctor's professional skills and experience, and patient's needs and desires. This paper introduces the concept and practice of EBM and explains how it can be used to solve the issues in stomatology.
Evidence-Based Medicine ; Humans ; Oral Medicine

ObjectiveTo explore the effects of personality characteristics and mental health of students on the career orientation.MethodsThe method of stratified random sampling was used in three universities of Jiangxi province.501 subjects of Arts,Science and Medical Science were tested with Holland career orientation test,symptom check list(SCL-90) and 16 personality factors(16PF).Structure validity analysis,analysis of variance and t -value test were employed with LISREL8.70 and SPSS13.0.Results ( 1 ) The proportion of students choosed the type of occupation were:social type 36％,management type 18％,conventional type 17％,reality type 14％,investigation type 9％,artistic type 6％ ; (2) Career orientation,personality characteristics and mental health had moderate effect on the career orientation.The correlation coefficients of personality characteristics and mental health to the vocational orientation were 0.63 and 0.44.The interaction correlation coefficient was 0.56.ConclusionPersonality characteristics and mental health of college students are important factors to the career orientation and decision-making of job seeking.The personality has stronger explanatory power than mental health.Great emphasis should be paid on both short-term psychological health of college students and long-term cultivation of healthy personality in the employment psychological guidance.

The separate blood information database meets the need of recording information outside the blood station, and it can send the collected information to the database of No.1 Military Medical Project.

Objective To clone and express the cDNA encoding Schistosoma japonicum tropomyosin. Methods The cDNA was amplified by reverse transcription polymerase chain reaction (RT PCR). The PCR products were ligated with pGEM T vectors and then for transformations. After characterization of white clones by agarose gel electrophoresis, endonucleases digestion and PCR, some recombinant plasmids with inserts were used for sequencing. Then the gene was subcloned into prokaryotic expression vector pQE30 and expression was induced by IPTG. Results The PCR products was 823 bp judged by agarose gel electrophoresis and sequencing. A cDNA encoding S japonicum tropomyosin, except for 14 amino acids at the amino terminus and 2 at the carboxyl terminus, has been constructed and cloned successfully. The colony, designated pGSjcTM12, was sequenced and shown to be 91 1% identical at the nuclei acid level and 98.1% identical in deduced amino acid sequence to that of S mansoni tropomyosin. The gene was subcloned into pQE30 and an expressed protein of about 32 kDa was obtained.Conclusion The cloning and expression of the gene encoding S japonicum tropomyosin had been successfully made.

OBJECTIVE:To establish a method for the simultaneous determination of paeoniflorin and ferulic acid in Naoxuesh-uan pill. METHODS:HPLC was performed on the column of Waters SunfireTM C18 with mobile phase of acetonitrile-water-glacial acetic acid(17:82:1,V/V/V)at a flow rate of 1.0 ml/min,detection wavelength was 230 nm,column temperature was 35 ℃,and the volume injection was 20 μl. RESULTS:The linear range was 0.024-0.240 mg/ml for paeoniflorin(r=0.999 9)and 0.009 4-0.141 0 mg/ml(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 95.00-97.90%(RSD=0.59%,n=6) and 95.29-98.24%(RSD=0.54%,n=6),respectively. CONCLUSIONS:The method is simple and repro-ducible,and can be used for the quality control of Naoxueshuan pill.