Natural Killer(NK)cytotoxicity of human PML coulcb be markedly increased by human umbilical-corld-blood-derived interferon-? (P-IFN) and Astragalus membranacesu (AIMember.)but slightly inhibited by high concentration of P-IFN (105u/ml) or A-Membr. (10mg/ml) .P-IFN and A.Membr., on the other hand, could protect target cells from NK cytotoxicity.However, this effect was lower than that of their enhancement of NK cytotoxicity.Moreover, P-IFN and A.Membr .could enhance each other.NK cytotoxicity might be increased by 5 to 6 times when the both agents were com-bined ia treatment of effector cells. A.Membr could induce interferon-r which mediated the enhancing effect of A.Membr NK cytotoxicity.There was a relationship be-tween enhancing effect of P-IFN on NK activity and large molecular metabolism of effector cells. It was supposed that interferon, except for activating NK cytotoxicity directly, might stimulate lymphocytes producing other kinds of factors by which interferon might further amplify its enhancing effect on NK cytotxicity.

OBJECTIVE:To establish HPLC method for the determination of the principal agent in phenoxymethylpenicillin potassium tablets.METHODS:The determination was performed on Diamonsil C 18 column with column temperature at(26?1)℃.The mobile phase consisted of acetonitrile-0.02mol/L sodium acetate(70∶30)with a flow rate at1.0ml/min.The detection wavelength was set at268nm;the sensitivity was0.01AUFS and the sample size was20?l.RESULTS:Good linear correlation with peak area was noted when the detection concentration range was10.0～120.0?g/mL for phenoxymethylpeni?cillin potassium(r=0.9999,n=5).The average recovery was100.2%(RSD=0.42%).CONCLUSION:The method was rapid,simple and accurate and it can be used to determine the content of the principal agent in phenoxymethylpenicillin potas?sium tablets.

Hospital information system is indispensable for hospital management,and its evaluation indexes system serves as the cornerstone for evaluation of hospital informationization.Basic evaluation indexes for hospital informationization were earmarked objectively as well as quantitatively and qualitatively,based on expert opinions.The evaluation indexes of hospital informationization consists of six dimensions and 23 secondary indexes.The paper described in detail the data makeup for reference of building the evaluation indexes system of hospital informationization.

OBJECTIVE:To survey and objectively evaluate the present situation and trend of the antihistamine agents used in 18 hospitals of Hangzhou.METHODS:To make a survey of the use of above mentioned drugs in 18 hospitals of Hangzhou during the period 2000～2002 in respect to the sum of money for drugs and manufacturing factories.RESULTS:In comparison with 2001,the sum of money for consumption of antihistamine agents increased 17.89%in 2000,and decreased 4.58%in 2002.Joint venture and imported products accounted for 70%of share of market.Lortadine and Cetirizine were still the first choice in therapy of allergic reactions.CONCLUSION:Consumption of antihistamine agents decreased,but kinds of drugs had no obvious change.

OBJECTIVE:To explore ways of promoting the rational use of antibacterials.METHODS:To analyze and e-valuate the effect of whole-ranged pharmaceutical care on promoting rational use of antibacterials.RESULTS:The whole-ranged pharmaceutical care was the efficient way to ensure the rational drug use of antibacterials.CONCLUSION:The whole-ranged pharmaceutical care should be carried out in hospital pharmacy so as to promote the rational use of antibacterials and to make the utmost use of the limited resource of Antibacterials.

OBJECTIVE To investigate the existance of class Ⅰ gene integron and transposon(merA and tnpA) gene marker in Escherichia coli isolated from the elderly patients.METHODS Antibiotic sensitivity was performed by microdilution test.Integron gene IntⅠ1 and transposon marker genes were detected by polymerase chain reaction(PCR).The PCR products were purified and sequenced.RESULTS E.coli resistant to amikacin accounted for 60%,to sulfamethoxazole 95%,and to third-generation cephalosporin 100%(all ESBLs-producing strains).The resistance was related closely to the class Ⅰ integron.The positive rate of integron intⅠ1 was 80%(16/20),whereas the positive rate of gene merA was only 10%(2/20).Gene tnpA was not tested out.CONCLUSIONS The drug resistance of E.coli has close relation with transmission of integron and transposon.The transposons of Tn21/Tn501 are all firstly reported in China.

Calponin is an actin filament-associated regulatory protein expressed in smooth muscle and many types ofnonmuscle cells.Three calponin isoforms 1,2,and 3 are encoded by three homologous genes CNN1,CNN2 and CNN3 in vertebrate species with cell type-specific expression and functions.Besides modulating the activity of smooth muscle myofilaments and contractility,calponin also regulates the functions of actin cytoskeleton in non-muscle cells during cell proliferation,adhesion,migration,differentiation,phagocytosis and fusion.This review focuses on the evolution,tissue and cell type-specific expression,structure-function relationships,transcriptional regulation and relevant mechanisms.

The corresponding astronomy implications of yin-yang and five phases of traditional Chinese medicine are investigated based on the modern astronomy,universal biology,and literature research.It is found that the principles of yin-yang in traditional Chinese medicine actually refers to the strengths and the directions of gravitational forces,electromagnetic waves,high energy particle flows of celestial bodies to human.The yin-yang in the human body is a result of selective evolution from protobiont under the influence of yin-yang of celestial bodies in hundreds of millions of years.The revolution of seven luminaries,which includes the Sun,Moon,Mercury,Venus,Mars,Jupiter and Saturn,creates the day and night yin-yang from the self rotation of the Earth,season yin-yang from the revolution of the Earth around the Sun,the yin-yao from the rotation of the Moon around the Earth,and the yin-yao from the rotations of the Mars and the Jupiter.The 10 years cyclic movement of the Mercury displays the states of jiayi,bingding,wuji,gengxin,rengui,which have the allelopathic characteristics of five phases-wood,fire,earth,metal and water,respectively.With the addition of yin-yang from the Mars,the states of Mercury are divided into yang five phases including jia,bing,wu,geng and ren,and yin five phases including yi,ding,ji,xin and gui.The yin-yang and five phases of human visceras is the result of the selective evolution under the influence of yin-yang and five phases of celestial bodies in hundreds of millions of years.The 5 years periodic movement of the Venus shows the five phases characteristics of the five zhongyun.With the addition of the yin-yang from the Mars,every zhongyun shows the yin-yang characteristics,which alternate once every other year.In a cycle of sixty years,the Saturn,Mars,Venus,Mercury and Jupiter show 15 pairs of independent yin-yang states,which is corresponding to the 15 pairs of yin-yang nayin five phases.

To improve the solubility of quercetin ( QT) , one of flavonoids that can inhibit the proliferation of vari-ous types of cancer cells, the novel amphiphilic polymer N-( 4-methylimidazole)-hydroxyethyl-chitosan ( MHC) , synthetized by chemical derivatization from chitosan, was used as the self-assembly micelles of QT. The formed polymer was characterized by 1 H NMR, elemental analysis and pyrene fluorescence spectrometry. The formulation of MHC micelles loading quercetin was optimized through single factor experiment. Then the optimized formulation was obtained as follows:the concentration of MHC was 0. 67% and the ratio of drug and carrier was 1 ∶10. The micelles particle size was ( 99. 21 ± 1. 71) nm, Zeta potential was +( 20. 01 ± 0. 72) mV and drug loading was ( 5. 42 ± 0. 32 )%. The in vitro release curve was investigated and was found to conform to Higuchi equation of Q=0. 1101 t1/2 -0. 064. The results of in vivo experiment showed that the mean rentention time and bioavail-ability of the MHC-QT micelles were 21. 42 h and 57. 49 μg h/mL, respectively, compared to 0. 30 h and 2. 50 μg h/mL of the free QT solution. These indicated that the MHC micelles could significantly improve the solubility of QT, the drug sustained-release effect and bioavailability, which would used as carrier for the anti-tumor drugs.

Aim To investigate the effect of intrathecal injection of PI3 K inhibitor LY294002 on pain behav-iour and expression of p-Akt in spinal dorsal horns in bone cancer pain( BCP) rats. Methods Forty female SD rats weighing 180~200 g were randomly divided in-to five groups ( n =8 each ):(Ⅰ) sham group;(Ⅱ) sham+LY294002 group;(Ⅲ) BCP group;(Ⅳ) BCP+DMSO group;( V) BCP+LY294002 group. BCP rat model was induced by inoculating Walker 256 mamma-ry gland carcinoma cells into the medullary cavity of the left tibia. Rats received i. t. injections of either PI3 K inhibitor LY294002 10μL ( 2. 5 g · L-1 ) or 5%DMSO 10 μL at the time of d 7~9 after the operation. Mechanical withdrawal threshold( MWT) test was per-formed before and after i. t. injections on d7(till 8h). The rats were sacrificed after inoculation and the L4~6 segments of the spinal cords were removed for immu-nohistochemistry to determinate the expression changes of spinal p-Akt. Results Compared with I group, the rats inⅢ,Ⅳ,Ⅴgroup showed obvious mechanical hy-peralgesia. The MWT of V group increased apparently from 2nd hour to 4th hour(P<0. 05),and reached the peak in 3rd hour(P<0. 01). Compared with I group, the expression of p-Akt in the spinal cord in Ⅲ,Ⅳgroup increased obviously ( P <0. 01 ) . Compared withⅢ,Ⅳ group,i. t. injections of LY294002 obviously cut down the expression of p-Akt in the spinal cord ( P <0. 05). Conclusion PI3K/Akt singaling pathway may take part in the development of bone cancer pain.