OBJECTIVE To discuss effective methods of nosocomial infection management in operating room to control the nosocomial infection and improve quality of medical care.METHODS To strengthen and improve the environment of operating room,and the management of medical staff,room cleaning and articles,as well as operation classification in there,to establish the effective nosocomial infection management organization in whole hospital,particulerly in operating room,to study technology of hospital infection,and to enhance the consciousness in control nosocomial infection.RESULTS It is very important guarantee that strengthen management of operating room work in hospital could improve the medical safety and prevent the nosocomicl infection.CONCLUSIONS To actualize scientific management in operating room,and control nosocomial infection.

Objective To research the risk of osteopo'rotic vertebral fractures with PKP after the PMMA injected location and dose of occurrence of vertebral refracture.Methods 78 cases of vertebral osteoporotic thoracolumbar vertebral compression fracture patients with PKP treatmentin were selected.According to position of postoperative PMMA in the vertebral body,they were divided into the side of the group of 35 patients and a median group of 43 patients.Compared two groups of patients before and after surgery,VAS,ODI score,the SI and Cobb angle and analyzed the PMMA leakage occur with the relationship of the amount of bone cement injected.Results There were significant differences in visual analogue pain scale(VAS),Oswestry disability index(ODI),sagittal index(SI) and Cobb angle of each group preoperatively versuspostoperatively (all P ＜ 0.05).No statistical difference was noted in the VAS,ODI,SI and Cobb angle between two groups preoperatively versus postoperatively(all P ＞ 0.05).PMMA injection volume between the the leakage group and the non-leakage patients had statistically significant difference (P ＜ 0.05).Conclusion PKP could obtain better clinical therapeutic effect in osteoporotic vertebral fractures treatment.Different PMMA positions had no significant influence on the therapeutic results.PMMA injected dose is a risk factor for bone cement leakage,which will increase the risk of vertebral fractures again.

Metebonomics is a branch of system biology,which,based on group index analysis, aims to analyze low molecular weight metabolites of the body both qualitatively and quantitatively through high flux and data processing.In recent years,the application of metabonomics to research of cardio-vascular pharmacology has been attracing more attention.This paper,by researching do mestic and overseas literature,summarizes the applications of metabonomics in the prevention and treatment of co mmon cardiovascular diseases such as hypertension,hyperlipide mia,atherosclerosis and myocardial ische mia.Related pharmacological researches targeted at patients,ani mal models and cell models clas-sified.Evidence for evaluation of effects and mechanis ms of drugs is provided by analyzing the patterns of change of metabolites in the progression of diseases.Finally,meaningful biomarkers are proposed.

Objective To conduct prospective randomized controlled clinical observation on patients with nasopharyngeal carcinoma under chemotherapy and radiotherapy using inhaling therapy with recombinant human interleukin 11,and the preventing and curing effect of recombinant human interleukin 11 on radiation-induced pharyngitis is evaluated.Methods 161 cases of newly diagnosed patients with head and neck cancer were selected under the principle of informed consent and these 161 cases were divided into the observation group (87 cases) and the control group (74 cases) based on randomization principle.In the observation group,when the dose reached 20Gy,then recombinant human interleukin-11 inhaling therapy was applied `ll the end of radiotherapy,for the control group,conventional treatment was applied in the event of radioactive throat.RTOG criteria were used during the observation in patients with the occurrence and treatment of radioactive pharyngitis.Results When compared with the control group,the average time for radioactivity to appear was prolonged,the severity decreased and the healing time was shortened in the observation group,those data were statistically significant.Conclusions Recombinant human interleukin-11 inhaling therapy can prevent and cure inhalation of radioactive pharyngitis and this therapy is better than conventional treatment.It's worthy of further study and clinical application.

Case teaching is one of the important means to improve teaching effect in the process of pharmacology teaching. According to the teaching object, teaching objectives and requirements to build one useful teaching case library about pharmacology, which can be used and shared for a long time, is the premise of case teaching in pharmacology. According to the actual situation of case teach-ing in our university, this study researched and explored the background of case library building, the basic principles of case compilation, the establishment of team, the teacher-led and the student-oriented processes of case building, and the process of sorting and summarizing cases in the case library build-ing, which promotes the reform in the pharmacology case teaching, improves teaching quality, and also provides a reference for other universities or schools and disciplines on teaching case library building.

Objective To characterize the foot pressure distribution during walking of the male college students with a normal left foot and toes-out right foot gait.Methods Forty-two male college students age 20 to 25 with a toes-out gait on the right side and a normal gait on the left side were recruited.The FOOTSCAN system was used to measure their foot pressure distribution while walking.Results There were significant differences between the normal and the toes-out foot with regard to the peak pressure on the third metatarsal bone [(45.05 ±13.91)N/cm2 vs (26.83 ± 10.82) N/cm2] and pressure under the arch [(4.48 ± 1.94) N/cm2 vs (2.90 ±1.57) N/cm2],as well as the time for the appearance of peak pressure under the 1st and 4th metatarsal bones.The foot regional impulse was significantly lower on the normal side than on the toes-out side for toes 2 to 5 and for metatarsal bone 2.Conclusion In contrast to the normal foot,the pressure center of the toe-out foot deflects to the inner side.This results in slanted power application instead of straight ahead,so the strength in the direction of travel is small.And it will produce torsion between the tibia and fibula,which makes the tibia appear introverted and causes excessive friction in the knee joint.This will lead to injury of the knee joint.

Objective To evaluate the effect of lipopolysaccharide (LPS) on thymocyte differentiation antigen-1 (Thy-1) mRNA expression in mouse lung fibroblasts.Methods Primary cultured mouse lung fibroblasts were seeded in 96-well plates with the density of 1 × 104/ml.After being cultured for 48 h,the cells were randomly divided into 4 groups (n =3 each):PBS control group (group C),LPS 0.01 μg/ml group (group LPS0.01),LPS 0.10 μg/ml group (group LPS0.10),and LPS 1.00 μg/ml group (group LPS1.00).PBS was added to the 96-well plates in group C.LPS with the final concentrations of 0.01,0.10 and 1.00 μg/ml were added to the 96-well plates in groups LPS0.01,LPS0.10 and LPS1.00,respectively.After being incubated for 0,6,24,48 and 72 h (T0-4),the proliferation of the cells was measured by CCK-8 assay and Thy-1 mRNA expression was detected by real-time PCR.Results Compared with group C,the proliferation of the cells was significantly increased,while Thy-1 mRNA expression was down-regulated at T3,4 in groups LPS0.01,LPS0.10 and LPS1.00 (P ＜ 0.05).The proliferation of the cells was gradually increased,while Thy-1 mRNA expression was gradually down-regulated in groups LPS0.01,LPS0.10 and LPS1.00 at T3,4 (P ＜ 0.05).Conclusion LPS results in abnormal proliferation of mouse lung fibroblasts through down-regulating Thy-1 mRNA expression,indicating that endoxemia-induced pulmonary fibrosis is related to the down-regulation of Thy-1 mRNA expression in lung fibroblasts.

Object To provide a basis for the identification of the crude drug of Fructus Schisandrae Sphenantherae Methods Morpholgical characters and microstructural features of the seed surface of Schisandra sphenanthera Rehd. et Wils , S. rubriflora (Franch ) Rehd. et Wils., S. viridis A. C. Smith, and S. henryi Clarke were observed under light microscope and scanning electron microscope. Identification of them was also completed by TLC qualitative analysis for deoxyschisandrin and schisantherin A Results The fruits of S. sphenanthera, S. rubriflora, S. viridis and S. henryi could be classified to three types with the characters of micromorphological features of the seed surface. The results of TLC showed that deoxyschisandrin and schisantherin A were present in the fruits of S. sphenanthera from Pingli, Shaanxi, Luanchuan, Henan, Yangcheng, Shanxi, S. rubriflora and S.viridis, Hengshan Hunan Provinces The fruit of S. henryi contained a little quantity of schisantherin A, but the fruits of S. sphenanthera from Liuba Shaanxi and Xiaolongshan Gansu Provinces did not have deoxyschisandrin and schisantherin A. Conclusion The fruits of S. sphenanthera from different geographical origin, S. rubriflora, S. viridis and S. henryi can be identified by the characters of micromorphological featrues of the seed surface and the TLC qualitative analysis for deoxyschisandrin and schisantherin A

Objective: To investigate the Ventral Pallidrm′s function of cardiovascular adjustment by observing the changes of the rats′blood pressure. Methods:Ten SD rats′blood pressure were respectively observed through a catheter in one of the common carotid arteries after unilateral Ventral Pallidum stimulation by certain concertration,certain volume glutamate solution under stereotaxic guidance. Results:Glutamate stimulation on Ventral Pallidum caused elevation of the rats′blood pressure,thechanges were significant. Conclusion:Ventral Pallidum has a role in regulating blood pressure. It belongs to behavior related pattern and sympathetic regulated.

Objective To study the MRI findings in a series of intracranial hemangiopericytomas, and to compare these findings with those of intracranial meningiomas. Methods The MRI appearances and clinical features in 27 cases of intracranial hemangiopericytomas and 236 cases of intracranial meningiomas were retrospectively reviewed. All cases were proved by operation and pathology. The data were evaluated by using ? 2 test, rank sum test, and Logistic Regression. Results Sex (? 2=6 981), shape of tumor (? 2=39 949), signal voids of vessel in tumor (? 2=12 366), necrosis or cystic degeneration in tumor (? 2=37 432), the type of dural attachment ( broad based or narrow based ) (? 2=16 956), T 1WI signal intensity (? 2=14 880), T 2WI signal intensity (? 2=37 068), enhanced degree of tumor (? 2=10 081), homogeneous degree of tumor after contrast enhancement (? 2=17 014), and duration time were significant different ( P