1.High level prokaryotic expression of neuron protective protein TAT-Bcl-XL and preliminary detection of its anti-apoptosis activity
Chinese Journal of Immunology 1985;0(06):-
Objective:To prepare the recombinant neuron protective protein TAT-Bcl-XL in E.coli expression system and to determine its anti-apoptosis activity.Methods:DNA fragment of TAT-Bcl-XL was obtained by RT-PCR using primers that were specific for Bcl-XL gene. Prokaryotic expression vector(pTBTOPO) was constructed by insertion of TAT-Bcl-XL DNA fragment into pCRT7/CT-TOPO vectors, and the recombinant protein was detected by SDS-PAGE and Western blot analysis using anti-V5 tag epitope antibody as the primary antibody. The recombinant fusion protein was purified by affinity chromatography, and the anti-apoptosis function of the purified protein was determined by flow cytometry.Results:A molecular weight of 30 000 protein was detectable in both SDS-PAGE and Western blot analysis. Immunofluorescent staining showed the fusion protein was distributed in cell plasma after incubation with 200 nmol/L fusion protein. Flow cytometry data indicated that the recombinant protein could enhance cell survival by 40% when 293T cells were treated with 250 ?mol/L zinc chloride as a supplementation in cell culture media.Conclusion:High level expression of the reported neuron protective protein was obtained in E.coli expression system, and the purified recombinant proteins remained its anti-apoptosis activity in our preliminary characterization.
2.Analysis of Adverse Drug Reactions Induced by Anti-epileptic Medicines in Intensive Care Unit of Neurology
China Pharmacy 2005;0(17):-
OBJECTIVE:To analyze the adverse drug reactions(ADR) caused by anti-epileptic medicines used for patients in the Neurological Intensive Care Unit (ICU) for reference of rational use of anti-epileptic medicines. METHODS: The ADR induced by anti-epileptic drugs between May 2006 and Nov. 2007 in neurological ICU patients with secondary epilepsy were analyzed retrospectively. RESULTS: A total of 24 ADR cases (24/50,47.06%) induced by anti-epileptic drugs were reported from Neurological Intensive Care Unit(ICU). The ADR were mainly induced by carbamazepine,valproate sodium,oxcarbazepine and phenobarbital,and the ADR mainly appeared as skin rash,hypolekocytosis,hepatic dysfunction and hyponatremia etc,but all cured after prompt treatment. CONCLUSION: The risk/efficiency ratio should be considered in clinical use of anti-epileptic drugs,and those drugs showing remarkable efficacy and few adverse reactions should be used preferably to reduce the damage to patients caused by ADR.
3.Monochromatic energy images from spectral CT in diagnosing X-ray negative biliary stones
Peipei XIE ; Weiling XUAN ; Jianping DING
Chinese Journal of Hepatobiliary Surgery 2016;22(7):460-463
Objective To study the role of monochromatic energy images from spectral CT in diagnosing X-ray negative biliary stones.Methods 32 patients who were diagnosed to have X-ray negative biliary stones were retrospectively studied.They were examined by spectral CT scanning and the spectral CT data were loaded into a spectrum analysis software.The optimal contrast-to-noise ratio (CNR) of the monochromatic energy images were exposed and the contrast ratio between the X-ray negative stones and bile on the optimal monochromatic and hybrid energy CT images were compared,respectively.The monochromatic and hybrid energy CT images for diagnosing X-ray negative biliary stones were validated by two senior radiologists based on postoperative histology.Results The corresponding KeV of optimal CNR for X-ray negative stones were not quite consistent.They were 140KeV in 19 patients,40KeV in 8 patients,53KeV,57KeV,62KeV,64KeV and 73KeV in one patient each.The contrast between the negative stones and the adjacent bile were (6.4 ±5.6) HU on hybrid energy CT images and (50.4 ±24.4) HU on optimal monochromatic energy CT images.The Eff-Z of negative stones and bile were 6.6 ± 0.6 and 7.9 ± 0.2,respectively.In our study,the diagnostic accuracy of hybrid energy CT images was 34.38% and that of optimal monochromatic energy CT images was 78.13%.Conclusion The optimal monochromatic energy CT images were more valuable in diagnosing X-ray negative bile duct stones,which were obviously better than the hybrid energy images from traditional CT.
4.Clinical investigation of the efficacy and safety of continuous intravenous Diltiazem for patients with refractory angina pectoris
Jianping LI ; Wenhui DING ; Junhua ZHANG
Chinese Journal of Interventional Cardiology 2001;9(1):31-33
Objective To investigate the efficacy of infusion Diltiazem for patients with refractory angina pectoris. Methods 10 patients with refractory angina pectoris received continuous intravenous Diltiazem 40~150 μg/min (2.4~9 mg/h) for 48 hours after cessation of intravenous nitroglycerin and oral β-blokers. Results With the single therapy of Diltiazem, 7 patients (70%) got satisfied results: 5 were free from and 2 were relieved of symptom; With the combination therapy of Diltiazem and intravenous nitroglycerin 60~120 μg/min after failed with Diltiazem alone, 3 (30%) patietns got satisfied results: 1 was free from and 2 were relieved of symptom. There is no severe side effects, including hypotension, bradycardia, cardiac function deterioration. Acute myocardial infarction, death and emergency interventional therapy did not occurred in all the 10 patients during the therapy period. Coronary angiography were performed in 8 patients within 1 week after the patients were stabled, 5 patients received PTCA and stent implantation and 3 patients received CABG. Conclusions Continuous intravenous Diltiazem 40~150 μg/min (2.4~9 mg/h) or combination with intravenous nitroglycerin 60~120 μg/min is an efficient and safe therapy for patients with refractory angina pectoris.
5.Effect of gadolinium chloride pretreatment on experimental hepatocarcinogenesis of H_(22) cells in mice
Mengyuan WANG ; Xiong DING ; Jianping GONG
Journal of Third Military Medical University 1988;0(05):-
Objective To investigate the effect of gadolinium chloride (GdCl3) on the H22 experimental hepatocarcinogenesis in mice. Methods Totally 80 mice were inflicted to experimental hepatoma by implanting H22 cells to their liver lobes, and then equally and randomly divided into experimental hepatoma group (B) and GdCl3 pretreatment group (10 mg/kg, C). Another 40 mice served as normal control group (A). Ten mice from every group were killed respectively 7, 14, and 28 d after implantation. The left 10 mice were used for recording survival time and measuring the mass weight. Hepatic pathological histology was observed, and the expression of TNF-? was detected by ELISA and RT-PCR. Results ①Survival time was obviously higher in group B than in group C (P
6.On Relationship Between Tort Liability and Special Relief for Adverse Drug Reactions
Jianping ZHANG ; Yong DING ; Xiaojie LOU
China Pharmacy 2007;0(34):-
OBJECTIVE:To study the relationship between Tort Liability and Special Relief for Adverse Drug Reactions (ADRs). METHODS: An analysis was conducted by literature review, logical reasoning and comparative study etc. RESULTS & CONCLUSIONS: In different country or area, the relationship between Tort Liability and Special Relief for ADRs manifested as German’s risk-sharing type, Japan’s supplement type, America’s mixed type. Tort Liability put an emphasis on the assignment and prevention of loss, while Special Relief on the sharing and shifting of risks. The functions of the two were different yet not incompatible, and they should work together to make up for the loss. Meanwhile, we should regard the trend of gradual socialization of the tort law seriously.
7.An experimental study on hepatocyte mitochondrium damage during acute biliary sepsis
Ding LUO ; Benli HAN ; Jianping GONG
Journal of Third Military Medical University 1988;0(06):-
In order to elucidate the mechanism of liver damage due to acute biliary sepsis,the changes of hepatocyte mitochondria were observed during biliary sepsis in the rat.The accompanied liver function changes were also studied.Mitochondrial calcium content,and lysosome fragility of the hepatocytes,lipid peroxide (LPO) level of liver tissue,ornithine carbamoytransferase (OCT),mitochondrial glulamicoxloacetic transaminase (m-GOT),and hepa-toplastin were determined.It was found that there were overloading of calcium in mitochondria,increase of lysosome fragility,and accumulation of LPO in the liver.These events would result in adverse effects on mitochondrial function.The activity of serum OCT and m-GOT was significantly increased,which suggests that mitochondria are seriously damaged since the 2 enzymes mainly come from hepatocyte mitochondria.And the liver reserving function declined progressively.Our study indicates that mitochondrial damage does exist during acute biliary sepsis,which might play an important role in liver damage.
8.A study of Kupffer cell phagocytic function during acute obstructive cholangitis in rats
Jianping GONG ; Bcnli HAN ; Ding LUO
Journal of Third Military Medical University 1988;0(06):-
The phagocytic function of Kupffer cells (KC) during acute obstructive cholangitis was observed in 244 Wistar rats.The rats were killed in the 6th,12th,24th,and 48th hour after operation,and the uptake of colloidal carbon by KC,plasma endotoxin and fibronectin(Fn)were determined and the morphology of KC was observed.It was found that in the rats with acute obstructive cholangitis,the phagocytic function of KC and plasma Fn significantly elevated in the 6th hour and markedly reduced in the 48th hour after operation as compared with those of the control (P
9.Changes of acid phosphatase and cytochrome oxidase in hepatic acini during acute obstructive cholangitis in rats
Jianping GONG ; Benli HAN ; Ding LUO
Journal of Third Military Medical University 1984;0(01):-
The changes of acid phosphatase(ACP)and cytochrome oxidase(CO)in the hepatic acini were investigated with image analysis in 90 Wistar rats after acute obstructive cholangitis was inflicted.It was found that the activity of ACP was significantly increased in the 2nd hour after surgery and markedly decreased in the 12th hour and these changes were predominantly located in acinur zone 1 of the hepatocytes.The activity of CO was decreased progressively after surgery,and these changes were quite marked in the acinur zone 3 of the hepatocytes.These findings indicate that the reactions towards acute obstructive cholangitis are different in the different acinur zones of the hepatocytes.
10.Effect of high volume hemofiltration on canine oleic acid lung injury
Jianping WU ; Yong GU ; Feng DING
Chinese Journal of Nephrology 1997;0(05):-
Objective To investigate the effects of high volume hemofiltration(HVHF) on cardiopulmonary functions and IL-?plasma levels in lung injury models. Methods Sixteen dogs with oleic acid-induced lung injury were randomly assigned into two groups(A and B). Group A served as control, undergoing mechanical ventilation only. Group B received HVHF and mechanical ventilation. Hemodynamic and respiratory mechanic variables were measured before and 0 mm, 120 mm, 240 mm after the induction of lung injury in both groups. Blood and ultrafiltrate samples were collected at the same time points. Plasma and ultrafiltrate concentrations of IL-? were measured by EUSA. Results There were significant differences between two groups on hemodynamic values for mean pulmonary arterial pressure(mPAP), pulmonary artery wedge pressure(PAWP), pulmonary vascular resistance(PVR) and mean arterial pressure(mABP) (P