OBJECTIVE To discuss effective methods of nosocomial infection management in operating room to control the nosocomial infection and improve quality of medical care.METHODS To strengthen and improve the environment of operating room,and the management of medical staff,room cleaning and articles,as well as operation classification in there,to establish the effective nosocomial infection management organization in whole hospital,particulerly in operating room,to study technology of hospital infection,and to enhance the consciousness in control nosocomial infection.RESULTS It is very important guarantee that strengthen management of operating room work in hospital could improve the medical safety and prevent the nosocomicl infection.CONCLUSIONS To actualize scientific management in operating room,and control nosocomial infection.

Case teaching is one of the important means to improve teaching effect in the process of pharmacology teaching. According to the teaching object, teaching objectives and requirements to build one useful teaching case library about pharmacology, which can be used and shared for a long time, is the premise of case teaching in pharmacology. According to the actual situation of case teach-ing in our university, this study researched and explored the background of case library building, the basic principles of case compilation, the establishment of team, the teacher-led and the student-oriented processes of case building, and the process of sorting and summarizing cases in the case library build-ing, which promotes the reform in the pharmacology case teaching, improves teaching quality, and also provides a reference for other universities or schools and disciplines on teaching case library building.

Objective To conduct prospective randomized controlled clinical observation on patients with nasopharyngeal carcinoma under chemotherapy and radiotherapy using inhaling therapy with recombinant human interleukin 11,and the preventing and curing effect of recombinant human interleukin 11 on radiation-induced pharyngitis is evaluated.Methods 161 cases of newly diagnosed patients with head and neck cancer were selected under the principle of informed consent and these 161 cases were divided into the observation group (87 cases) and the control group (74 cases) based on randomization principle.In the observation group,when the dose reached 20Gy,then recombinant human interleukin-11 inhaling therapy was applied `ll the end of radiotherapy,for the control group,conventional treatment was applied in the event of radioactive throat.RTOG criteria were used during the observation in patients with the occurrence and treatment of radioactive pharyngitis.Results When compared with the control group,the average time for radioactivity to appear was prolonged,the severity decreased and the healing time was shortened in the observation group,those data were statistically significant.Conclusions Recombinant human interleukin-11 inhaling therapy can prevent and cure inhalation of radioactive pharyngitis and this therapy is better than conventional treatment.It's worthy of further study and clinical application.

Objective To research the risk of osteopo'rotic vertebral fractures with PKP after the PMMA injected location and dose of occurrence of vertebral refracture.Methods 78 cases of vertebral osteoporotic thoracolumbar vertebral compression fracture patients with PKP treatmentin were selected.According to position of postoperative PMMA in the vertebral body,they were divided into the side of the group of 35 patients and a median group of 43 patients.Compared two groups of patients before and after surgery,VAS,ODI score,the SI and Cobb angle and analyzed the PMMA leakage occur with the relationship of the amount of bone cement injected.Results There were significant differences in visual analogue pain scale(VAS),Oswestry disability index(ODI),sagittal index(SI) and Cobb angle of each group preoperatively versuspostoperatively (all P ＜ 0.05).No statistical difference was noted in the VAS,ODI,SI and Cobb angle between two groups preoperatively versus postoperatively(all P ＞ 0.05).PMMA injection volume between the the leakage group and the non-leakage patients had statistically significant difference (P ＜ 0.05).Conclusion PKP could obtain better clinical therapeutic effect in osteoporotic vertebral fractures treatment.Different PMMA positions had no significant influence on the therapeutic results.PMMA injected dose is a risk factor for bone cement leakage,which will increase the risk of vertebral fractures again.

Metebonomics is a branch of system biology,which,based on group index analysis, aims to analyze low molecular weight metabolites of the body both qualitatively and quantitatively through high flux and data processing.In recent years,the application of metabonomics to research of cardio-vascular pharmacology has been attracing more attention.This paper,by researching do mestic and overseas literature,summarizes the applications of metabonomics in the prevention and treatment of co mmon cardiovascular diseases such as hypertension,hyperlipide mia,atherosclerosis and myocardial ische mia.Related pharmacological researches targeted at patients,ani mal models and cell models clas-sified.Evidence for evaluation of effects and mechanis ms of drugs is provided by analyzing the patterns of change of metabolites in the progression of diseases.Finally,meaningful biomarkers are proposed.

Objective To compare the effects of closed reduction and interlocking intramedullary nail and percutaneous minimally invasive locking compression plate in treatment of tibial fractures.Methods 70 patients with tibial fractures were selected as research subjects,and they were randomly divided into the control group and the observation group according to the digital table,each group in 35cases.The control group was treated with closed reduction and interlocking intramedullary nail,while the observation group was treated with percutaneous minimally invasive locking compression plate.Then,the operation index,postoperative complications,treatment effect after treatment between two groups were compared.Results The operation time,fracture healing time,length of stay in the observation group were less than those in the control group[(70.37±11.06)min vs.(82.13±12.15)min,(3.02±0.61)month vs.(4.21±0.70)month,(13.39±3.02)d vs.(16.26±3.21)d](t=4.234,3.852,7.582,all P<0.05).The incidence rate of complication in the observation group was lower than that in the control group[14.28%(5/35) vs.51.42%(18/35)](χ2=10.943,P<0.05).The excellent rate in the observation group was statistically higher than that in the control group[94.28%(33/35) vs.74.28%(26/35)](χ2=5.285,P<0.05).Conclusion Closed reduction and interlocking intramedullary nail and minimally invasive percutaneous locking compression plate are successful in the treatment of tibial fracture,but interlocking intramedullary nail easily leads to delayed healing,complications and higher rate of surgery two times,while minimally invasive percutaneous locking compression plate can promote healing,curative effect is better.

Objective To characterize the foot pressure distribution during walking of the male college students with a normal left foot and toes-out right foot gait.Methods Forty-two male college students age 20 to 25 with a toes-out gait on the right side and a normal gait on the left side were recruited.The FOOTSCAN system was used to measure their foot pressure distribution while walking.Results There were significant differences between the normal and the toes-out foot with regard to the peak pressure on the third metatarsal bone [(45.05 ±13.91)N/cm2 vs (26.83 ± 10.82) N/cm2] and pressure under the arch [(4.48 ± 1.94) N/cm2 vs (2.90 ±1.57) N/cm2],as well as the time for the appearance of peak pressure under the 1st and 4th metatarsal bones.The foot regional impulse was significantly lower on the normal side than on the toes-out side for toes 2 to 5 and for metatarsal bone 2.Conclusion In contrast to the normal foot,the pressure center of the toe-out foot deflects to the inner side.This results in slanted power application instead of straight ahead,so the strength in the direction of travel is small.And it will produce torsion between the tibia and fibula,which makes the tibia appear introverted and causes excessive friction in the knee joint.This will lead to injury of the knee joint.

Objective To evaluate the effect of lipopolysaccharide (LPS) on thymocyte differentiation antigen-1 (Thy-1) mRNA expression in mouse lung fibroblasts.Methods Primary cultured mouse lung fibroblasts were seeded in 96-well plates with the density of 1 × 104/ml.After being cultured for 48 h,the cells were randomly divided into 4 groups (n =3 each):PBS control group (group C),LPS 0.01 μg/ml group (group LPS0.01),LPS 0.10 μg/ml group (group LPS0.10),and LPS 1.00 μg/ml group (group LPS1.00).PBS was added to the 96-well plates in group C.LPS with the final concentrations of 0.01,0.10 and 1.00 μg/ml were added to the 96-well plates in groups LPS0.01,LPS0.10 and LPS1.00,respectively.After being incubated for 0,6,24,48 and 72 h (T0-4),the proliferation of the cells was measured by CCK-8 assay and Thy-1 mRNA expression was detected by real-time PCR.Results Compared with group C,the proliferation of the cells was significantly increased,while Thy-1 mRNA expression was down-regulated at T3,4 in groups LPS0.01,LPS0.10 and LPS1.00 (P ＜ 0.05).The proliferation of the cells was gradually increased,while Thy-1 mRNA expression was gradually down-regulated in groups LPS0.01,LPS0.10 and LPS1.00 at T3,4 (P ＜ 0.05).Conclusion LPS results in abnormal proliferation of mouse lung fibroblasts through down-regulating Thy-1 mRNA expression,indicating that endoxemia-induced pulmonary fibrosis is related to the down-regulation of Thy-1 mRNA expression in lung fibroblasts.

The article reviewed the situation of warming kidney and strengthen spleen treatment for ulcerative colitis (UC) in recent 15 years from three aspects: Yang deficiency in spleen and kidney syndrome is one of the most important syndromes in UC; common prescriptions, medicine and efficacy of warming kidney and strengthening spleen treatment for treating UC; the mechanism of the treatment for UC is related to immunity enhancement, inhibition of gastrointestinal smooth muscle, promoting gastrointestinal function, antibiosis, improving plant nerve function, and release of enzymes. It also proposed the problems about warming kidney and strengthening spleen treatment for UC, and prospect for relevant problems.

Objective To investigate the clinical significance of WT1 gene expression in the urine. Methods The expression of WT1 gene in the urine of patients with chronic glomerular nephritis(CGN), rheumatic diseases,diabetes mellitus(DM) and healthy subjects was detected by semi-nested PCR. Results The positive rates of WT1 gene expression in CGN with proteinuria,DM with proteinuria, DM without proteinuria and rheumatic diseases were 46.7% (14/30),44.4% (16/36),5.95% (5/84) and 16%(4/25), respectively. WT1 expression in the urine was not related to the degree of proteinuria, hematuria, renal function or clinical course of diseases. WT1 expression in the urine of the healthy subjects or cystitis patients was negative. Conclusion The measurement of WT1 mRNA in the urine could play an important role in early diagnosis of progressive renal damage.