Objective To investigate the expression of vascular endothelial growth factor (VEGF)、matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9 (MMP-9) in tongue squamous cell carcinoma and the clinical significance.Methods The expression of MMP-2,MMP-9 and VEGF in 60 cases of tongues quamous cell carcinoma tissues and in 20 cases of normal tongues tissues were examined by immunochemistry.Results The expression of MMP-2,MMP-9 and VEGF was higher in tongues quamous cell carcinoma than that in normal pancreatic parenchyma.In cases with lymph nodes metastasis and TNM stage Ⅲ + Ⅳ,the expression of two markers were significantly higher than those without lymph nodes metastasis and TNM stage Ⅰ + Ⅱ (P ＜ 0.05).A positive correlation was found between the expression of VEGF and MMP-2,MMP-9 in tongues quamous cell carcinoma ( P ＜ 0.05 ).Conclusion The expression of MMP-2,MMP-9 and VEGF was closely correlated with lymph nodes metastasis and TNM stage of tongues quamous cell carcinoma(P ＜ 0.05 ).VEGF and MMP-2,MMP-9 play an important role in the development of tongues quamous cell carcinoma,may cooperate with each other.

The Chinese herbal compound formula preparation,which is guided by traditional Chinese medicine (TCM) theories,and based on long-term clinical application,has its unique value.The multi-compound and multi-target characteristics are also features of Chinese materia medica (CMM).The finding and exploration of innovation CMM in modern times should be based on TCM theory and clinical application,as well as the combination of efficacy in the concept of holism and biomedical technology.Key issue in the research,development and evaluation of innovation Chinese medicine is to use to the methodology to illustrate the scientific meaning in according to the clinical application characteristics of Chinese herbal compound formula preparation.During the research and development process,considerations on CMM characteristics should be taken from research evidence,to pharmacodynamics and toxicology studies.Pharmacodynamics researches should reflect the properties of TCM syndrome.Aspects,such as research and application of TCM syndrome model,correspondence between disease and syndrome model,research method of correspondence between syndrome and prescription,identification of biological markers corresponding to CMM pharmacologic effect,are important.So we should catch up the historical chance for TCM development,speedily develop the advantage areas,and promote the modernization and innovation of TCM.

[Objective]To explore effect of polypeptides from chlamys farreri(PCF) on human umbilical cord blood cells(HUCBCs) differentiation in vitro,the effects of the extract on protein tyrosine kinase(PTKs) was studied in vitro.[Method]Getting human umbilical cord blood cells and cultured with the PCF at dilution concentrations of 1:1600,1:3200,1:6400,1:12800 nerve growth factor(NGF) was used as positive control and DMEM as negative one.After 48,72,96 h,the HUCBCs was cultured and the activity of protein tyrosine kinase(PTK) was examined by ATP in corporation test using PtK assay system.[Result]HUCBCs in the negative control group had no PTK activity,but that of positive control group was detected significantly.The PTK activity in extract of polypeptides from chlamys farreri groups increased at 48h(P

Aim To investigate the protective effects of huang lian jie du tang active fraction(HLJDTAF) on ischemia injury in cerebral cortical neuronal cultures. Methods The cortical neurons of rat fetal were cultured in vitro. The protective effects of HLJDTAF on ischemia injury were observed by treating cells with sodium dithionite in glucose free medium. Results HLJDTAF 610 mg?kg -1 increased the activity of neuron, 305 mg?kg -1 reduced the leakage rate of LDH; HLJDTAF 610, 305, 153 mg?kg -1 reduced the content of NO,MDA and increased the activity of SOD. Conclusion HLJDTAF protected the cerebral cortical neurons from ischemia injury by reducing the contents of NO and suppressing the generation of lipidperoxide.

Objective To study the protective effect of Duzhong Hongjingtian Capsule (DHC) on cerebral ischemia in rats. Methods Thrombotic ischemia and middle cerebral artery occlusion (MCAO) induced by FeCl3 in rats was used to observe the protective effect of DHC on ischemia stroke, and common carotid artery occlusion (CCAO) rats was used to analyze the effect of DHC on blood brain barrier (BBB) permeability. Results DHC could improve the abnormal behavior of MCAO rats and decrease the infarction area and water content in the brain of MCAO rats. DHC could also improve the cerebral blood flow on MCAO rats and significantly reduce the permeability for evans' blue (EB) of CCAO rats. Conclusions DHC can protect the brain ischemic injury.

Diabetic cardiomyopathy ( DC) is an independent complication of diabetes mellitus accompanied with cardiac metabolic imbalances ( increase of fatty acids and reduction of glucose) ,which would impair cardiac function and structure seriously. Peroxisome proliferator-activated receptors ( PPARs) are ligand-activated transcription factors belonging to the nuclear hormone receptor superfamily,and they could transcriptionally regulate energy metabolism and function. PPARs also play an important role in regulating metabolism in DC heart,and directly or indirectly affect cardiac function and structure.

Alzheimer ’ s disease ( AD ) is characterized by pro-gressive loss of memory and other cognitive functions .With re-cent discoveries , activation of silent mating-type information reg-ulator 2 homolog 1 ( SIRT1 ) could attenuate the cognitive dys-function of AD via reducing amyloid-βaggregation and tau pro-tein phosphorylation , inhibiting inflammatory reaction , and regu-lating synaptic plasticity .This review aims to highlight the in-volvement of these new discoveries of SIRT 1, and Akt/protein kinase B(PKB) signaling pathways, for their potential therapeu-tic effect against AD .

Objective To investigate the significance of administration of ionic contrast media with dexamethasone in preventing side effect during enhanced CT scanning. Methods We prospectively studied 520 patients who had enhanced CT scans and divided them into two groups: 259 patients received ionic contrast medium with dexamethasone (dexamethasone group); 261 patients received ionic contrast medium without dexamethasone (contrpl group). The side effects of the patients in two groups with different ages were compared. Results There were 19 patients with side effects in dexamethasone group (7 3%), 20 patients with side effect in control group(7 7%). The side effect rate in those patients over the aged 20 years and under the aged 60 years was higher than other age groups. The side effect rate was similar between dexamethasone group and control grouop.Conclusion Administration of Ionic contrast media with dexamethasone during CT scanning can not obviously decrease the incidence of side effect.

ObjectiveTo compare the health-related quality of life (HRQOL) of patients with or without coronary artery disease (CAD) and also to determine the factors affecting their quality of life.MethodsOne hundred patients with CAD and 100 patients without CAD were included in this cross-sectional study. The patients with CAD and the patients without CAD were matched by age, sex, marital status, and income. HRQOL was measured with the generic HRQOL instrument.ResultsHRQOL scores of the patients with CAD differed significantly from the patients without CAD. Compared to the patients without CAD, the HRQOL of the patients with CAD was lower in the dimensions of mobility, hearing, breathing, elimination, usual activities, mental function, discomfort and symptoms, vitality, and sexual activity. The patients with CAD had lower HRQOLscores than the patients without CAD. In the patients with CAD, age and sex independently affected the HRQOL. Older patients and women had worse HRQOL.ConclusionCoronary artery disease negatively affects HRQOL. It is thought that interventions to increase quality of life the patients with CAD may improve their HRQOL.

Objective To explore influence of out-hospital health education on life quality of senile patient with acute myocardial infarction (AMI) after underwent stenting therapy of coronary artery.Methods A total of 100 cases of AMI patients undergoing stenting therapy of coronary artery were selected and randomly divided into the follow-up group and control group with 50 cases in each group. Patients of the follow-up group accepted out-hospital health education. While patients of the control group were only carried out conventional in-hospital health education. The quality of life of two groups was compared.Results At the end of study, the follow-up group had a better life quality than the control group ( P<0.01). The satisfactory degrees of nurse-patient relationship in the follow-up group were better than that of control group ( P<0.05).Conclusion Out-hospital health education can improve life quality of senile patient with AMI after underwent stenting therapy of coronary artery.