The T helper cell 17 (Th17) has important relationship with liver failure caused by hepatitis B virus (HBV).Some studies found that mature,differentiation and proliferation of Th17 cells have a close relationship with interleukin 23,newly discovered in recent years,which function of immune and regulatory mechanism in chronic hepatitis B patients is still unclear.In our country,the main cause of liver failure is hepatitis viruses (mainly HBV),thus the study of immune pathogenesis that why acute exacerbation or liver failure happens in chronic hepatitis B patients has a realistic meaning for improving the prognosis of chronic hepatitis B.

Wnt signal transduction pathway plays a very important role in embryonic development, and its abnormality may lead to developmental defects and cellular malignant transformation. It has been shown that disturbances of the Wnt signal transduction pathway were significantly related to human neoplastic transformation. The core mechanism of the pathway abnormality was the accumulation of free ?-catenin protein in the cytoplasm and nucleus resulting in the expressions of specific target genes by downstream pathway. This paper reviews the composition and structural character, transduction and regulation of the Wnt signal pathway and its relation with human tumorigenesis.

BACKGROUND:Liver cancer pathogenesis and intervention have attracted increasing attentions. Mesenchymal stem cel s become a popular tool for cel cancer research, because of their low immunogenicity and tumor tropism. At present, mesenchymal stem cel s have been applied to the study of liver cancer.
OBJECTIVE:To summarize the advances of mesenchymal stem cel s used in liver cancer in basic and clinical research.
METHODS:An online retrieval of CNKI and Pubmed database was performed by the first author for the articles about mesenchymal stem cel s and effect of modified mesenchymal stem cel s on hepatoma carcinoma cel s published from January 2004 to January 2013. The key words were“mesenchymal stem cel , liver cancer, tumor”in Chinese and English. Repetitive research was excluded, and 47 studies met the inclusion criteria.
RESULTS AND CONCLUSION:Stem cel s are seldom reported in liver cancer, and the limited present study show that mesenchymal stem cel s may have a certain influence on the hepatoma carcinoma cel proliferation, invasion and biological behavior. However, due to the differences of cel lines used by the various laboratories, experimental conditions, animal models, as wel as infusion means of stem cel s, experimental results are also inconsistent. Scholars have conducted a series of studies on the mechanism of the Wnt pathway and tumor necrosis factor-related apoptosis-inducing ligand pathway. Tropism of mesenchymal stem cel s to tumor cel s, including liver cancer is widely recognized, so scholars imported therapeutic genes and drugs into mesenchymal stem cel s to interfere with the development of liver cancer, and have achieved some progress. This evidence provides new avenues for cel therapy for liver cancer. Less safety studies in vivo and clinical trials of mesenchymal stem cel s are available, therefore security risks deserve further research.

[Objective]Extracellular signal-activated kinases(ERK),c-Jun N-terminal kinase(JNK) and P38 signal transduction pathway were three members of the MAPK family which mediated cellular proliferation,differentiation and apoptosis.The purpose of this study was to investigate the effects of ERK,JNK and P38 MAPK on pathogenesis and progression of human osteosarcoma.[Method]EnVisionTM immunohistochemical technique was used to detect the expressions of ERK,JNK and P38 protein among the 48 osteosarcoma and 25 osteoblastic benign tumor samples,and their differences were compared.[Result]The positive rates of ERK,JNK and P38 protein were 83.3%(40/48),72.9%(35/48) and 85.4%(41/48) respectively in 48 osteosarcoma,and 16.0%(4/25),12.0%(3/25) and 20.0%(5/25) in 14 osteoblastic benign tumor,the statistical analysis of the positive rates were performed using analysis of variance with P

Objective To Inves tigate the blood lead levels of children of 1/12～13 years old in Wenzhou and early diagnose lead poisoning in them.Methods The whole blood lead levels of 2956 children aged 1/12～13 years were determined by atom absorbspectral analysis.Results The mean of all blood lead levels was(63.11?30.17)?g/L (2037 boys were 65.55?31.23?g/L,919 girls were 57.72?26.78?g/L),318 children(10.77%) were with a blood lead ≥100?g/L(259 boys,59 girls).The prevalence of lead poisoning in boys(12.7%)as higher than those in girls 6.4%(?~2=18.99,P

Objective: To study the apoptosis and the expression of proliferating cell nuclear antigen (PCNA) in colorec-tal neoplasms and their relationship with neoplasm biological behavior. Methods: Apoptotic index (AI) and proliferative index (PI) were determined in paraffin-embedded tissues from 62 cases of colorectal carcinomas, 15 cases of colon adenomas and 10 cases of normal colon mucosa with immunohistochemistry S-P method and TUNEL technique. Results: The difference of PI and AI was significant in normal colon mucosa, colon adenomas and colorectal carcinomas (P

Objective To study the expression of fascin, an actin bundling protein associated with cell motility, and the relationship with ER negative breast carcinoma.Methods Comparasion of the expression of fascin, estrogen receptor (ER) and proliferative cell nuclear antigen (PCNA) determined by immunohistological method was made on 84 specimens of primary breast carcinomas. Results The diffuse staining of fascin in cancerous cell cytoplasm was noted. Only 3 of 53 breast carcinomas with ER positive showed fascin positive expression (5.67%); in contrast, 21 of 31 carcinomas with ER-negative showed positive expression(67.7 %) (P

Dendritic Cells ; pathology ; Humans ; Leukemia, Myeloid ; pathology ; Plasmacytoma ; pathology

BACKGROUND: Recent data suggested that Notch signal pathway plays important regulatory effects in peripheral transplantation immunological response, promotes differentiation of regulatory T cells, induces antigen specific immune tolerance. We proposed that Notch/Notch ligand may play important roles in MHC/TCR interface.OBJECTIVE: To construct the eukaryotic expression vector of rat Deltai gene (Notch ligand), and to examine its expression in dendritic cells.METHODS: The complete encoding cDNA of rat-Delta1 was isolated from bone marrow cells by reverse transcription-polymerase chain reaction (RT-PCR) and this gene was recombined into pcDNA3.1(+) plasmid vector.pcDNA3.1/Delta1 plasmid was transfected into rat dendritic cells with lipofectamine gene transfection method.RESULTS AND CONCLUSION: Double enzyme digestion detection demonstrated that Delta 1 had been successfully constructed in Hindilll and xbal of pcDNA3.1. A positive clone pcDNA3.1/Delta1 was delivered to Shanghai Sangon Biological Engineering Technology & Services Co., Ltd. for sequencing. Sequencing results were identical to Delta1 gene sequence in Genebank, with correct reading frame. The Delta 1 gene-transfected dendritic cells showed similar morphology as their parent cells. Western blotting assay detected that Delta 1 expression was significantly increased in cells. The eukaryotic expression vector pcDNA3.1/Delta1 was constructed, and significant increase of Delta 1 expression was detected after transfection.