【Objective】To observe the effect of ?-asaricin spray on guinea pigs bronchial asthma.【Methods】Thirty FMMU guinea pigs were equally randomized into three groups:A(treated with ?-asaricin spray),B(treated with aminophylline)and C(model).Ater 7-day treatment,the guinea pigs received nebulization of histamine and acetylcholine to induce asthma models.And the effect of ?-asaricin on asthmatic attack latency and fall latency in guinea pigs was observed.The isolated trachea was used to observe the effect of ?-asaricin on tension of isolated bronchial smooth muscle.Other 30 guinea pigs were also randomized into three groups.The guinea pigs were sensitized with 40?g/L ovalbumin and Al(OH)3 firstly,treated with nebulization therapy for 7 days,and then induced asthma by nebulization of ovalbumin to observe the effect on the degranulation of mastocytes in bronchus and lung tissue.【Results】?-asaricin prolonged asthma latency and fall latency,inhibited the degranulation of mastocytes induced by ovalbumin and Al(OH)3,and promoted the relaxation of bronchial smooth muscle induced by histamine and acetylcholine,the difference being significant(P

The proto-oncogene, c-kit gene, widely expresses on the mast cell, melanoeyte, haemopoietic stem cell, intestinal cell of cajal and germoeyte. Recent researches have revealed the relationship between the e-kit gene and the carcinogenesis, proliferation, infiltration and metastasis of some malignant tumor. This article aimed to make a review of its biological function, lab and clinical research advancement in the digestive tract tumor.

The traditional single-disciplinary treatment has been transformed into a multi-disciplinary comprehensive treatment,because of the further understanding of oncology.Multi-disciplinary team (MDT) is the representative of this change.MDT has a unique model of organization and conduction.It improves the prognosis of patients through multi-disciplinary conference and standardized individualized treatment.MDT has been applied in metastatic colorectal cancer for a long time,and remarkable achievements have been made.With the development of molecular biology and the application of new targeted agents,MDT has also been progressing to adapt to the trend of modern medicine.

The da Vinci robotic surgical system assisted colorectal radical resection has been widely applied in many countries and regions.The advantages of the da Vinci robotic surgical system include stable and distinct stereoscopic vision,short learning curve,convenient operation and feasibility of remote manipulation,while its disadvantages are expensive cost,lack of tremor and prolonged operation time.The advantage of da Vinci robotic surgical system is not distinct when compared with traditional laparoscope in radical resection of colorectal cancer.With the accumulation of clinical data and the update of the system,the prospect of da Vinci robotic surgical system assisted colorectal radical resection is promising.

Liver metastasis is the leading cause of death from colorectal cancer.Surgical treatment is so far the best choice for the treatment of colorectal liver metastases (CRLM).However,how to suitably select an individualized chemotherapy in perioperative period and surgical time and tool is still under controversy.Therefore,the key factors of improving the prognosis of patients with CRLM include overall evaluation of the patients and their preoperative examination outcomes,dividing patients into different groups based on different therapeutic goals,understanding different therapeutic goals of each group,selecting suitable chemotherapy and targeted therapy,ensuring surgical time (synchronous or stepwise resection) and tools (minimally invasive or open surgery) through multidisciplinary team model.

Objective To study the clinical significance of the expression of matrix metalloproteinase-9(MMP-9) and E-cadherin(E-cad) in patients with non-small cell lung cancer (NSCLC).Methods Immunohistochemical method (SP) was used to detect the expression of the MMP-9 and E-cad in 62 NSCLC tissues and 18 normal lung tissues.Results The positive expression rate of MMP-9 and the abnormal expression rate of E-cad in NSCLC were closely related to the TNM stages,pathological grades and lymph node involvement (P

Objective To summarize the bacterial drug susceptibility of common enterobacteriaceae in pediatric patients and the epidemic status of the extended spectrum beta‐lactamases(ESBLs) during 2013 and 2014 in our hospital in order to guide the clini‐cal application of antibacterial drugs .Methods Routine methods of isolation and culture were performed and the distribution of en‐terobacteriaceae with resistance were statistically analyzed with the software .Results A total of 530 isolates were collected in the hospital in which was 32 .08% Klebsiella pneumonia and 38 .87% Escherichia coli .The most sensitive drug of Enterobacteriaeeae was carbapenems .ESBLs positive rate in Escherichia coli and Klebsiella pneumonia were 32 .04% and 20% .The difference was sta‐tistically significant (P<0 .05) .Conclusion Escherichia coli and Klebsiella pneumonia were the common enterobacteriaceae in pedi‐atric patients .Carbapenems remained very high activity against Enterobacteriaceae .However ,the carbapenems resistance strains have been appeared that should pay attention .Antibiotic therapy should be based on the guidance of bacteriology to select sensitive drugs .

Liver metastasis is the leading cause of death from colorectal cancer (CRC).Multidisciplinary team model has become standard strategy for the diagnosis and treatment of colorectal liver metastases (CRLM),making individualized treatment through precise staging and molecular typing.Preoperative hepatic and regional arterial infusion chemotherapy and portal vein chemotherapy during surgery can reduce the incidence of liver metastases for stage Ⅲ CRC.Surgical resection of CRLM has been accepted by all scholars,and can be performed by minimally invasive surgery.The criteria for hepatic resection of CRLM have been extended including complete tumor resection,negative resection margin and adequate remnant liver volume.Simultaneous resections of primary tumor and liver metastases are safe and feasible.However,neoadjuvant chemotherapy is still controversial.For unresectable liver metastases,conversion therapy should be implemented to make the liver metastases become resectable.For unconvertible and unresectable CRLM,aggressive and comprehensive treatment is required to control the disease progression.

Sphingosine-1-phosphate (S1 P) is a kind of bioactive messenger which is catalyzed by sphingosine kinases (SphK) and mediates various biological behaviors through binding to sphingosine-1-phosphate receptors(S1PR).Researches demonstrate that the aberration of SphK,S1P and S1PR is closely related to the occurrence and development of cancers,such as leukemia,breast cancer,colorectal cancer and lymphoma.The interaction between S1PR1 and signal transducer and activator of transcription 3 (STAT3) is shown to take part in the pathogenesis of malignant tumors as well.Therefore,they are considered to have the potential tumor-treatment value.

Objective To investigate the indication, preoperative evaluation, technical tip, prevention and treatment of complications, effect, and clinical use of the embolization of intracranial aneurysms by using GDCs. Methods From July 1998 to February 2000,93 patients with 118 intracranial aneurysms were embolized using GDCs, including 84 ruptured aneurysms (Hunt-Hess grade, Ⅰ 18 cases, Ⅱ 29 cases, Ⅲ 25 cases, Ⅳ 10 cases, Ⅴ 2 cases). 72 patients were treated at emergency, and 48 patients had CTA examination preoperatively. The operations were completed under the DSA monitoring. Continuous lumbar subarachnoid drainage was instituted and standard 3H treatment was given postoperatively. Results Total occlusion was achieved in 104 aneurysms(88 14%), over 90% in 11aneurysms(9.32%), less than 90% in 3 aneurysms(2 54%), with 3 patients (3/93, 3 23%) died in the cohort. One case rebleeded after 1.5 months and was cured by surgical clipping, 2 patients experienced enlargement of the aneurysmal neck and were reembolized. Following-up was performed from 2 to 22 months, 1 had severe disability, 7 had mild neurological deficit symptoms. Conclusion The results of short-term follow-up suggested that embolization of intracranial aneurysms with GDCs be microtraumatic, safe, effective, and reliable. Partial packing may result in continuous enlargement, rupture, and bleeding, while tight coil packing in aneurysms was reliable. The ruptured aneurysms need emergency embolization, and postoperative continuous lumbar subarachnoid drainage can enhance curative effect significantly.