Objective To evaluate the value of brain natriuretic peptide(BNP) in estimating risk stratification in patients with acute myocardial infarction(AMI) and to determine the relationship between BNP and adverse cardiac events after AMI.Methods The 135 subjects were selected into the study including 25 healthy subjects and 110 patients with a first AMI.The plasma concentrations of BNP were measured at two to four days after infarction in patients and healthy controls.Left ventricular function was evaluated by echocardiography with the parameters of left ventricular ejection function(LVEF) after 3 months.Patients were followed up at 12 months.The main outcome measures were heart failure,left remodeling mortality and other adverse cardiac events at one year.Results Plasma BNP concentrations in patients with AMI were much higher than those in the health control people[(416.7?208.0)ng/L versus (61.8?34.1)ng/L,P400ng/L,P=0.019).Plasma BNP concentrations remained associated with the development of clinical end points in multivariable model that adjusted for potential confounding variables.Conclusion The results of the present study confirm that the elevated BNP count related to the risk stratification and prognosis in patients with AMI elevations in BNP count is associated with a higher incidence of new CHF and adverse clinical outcomes after AMI it could serve as s strong predictor for the subsequent development of poor outcomes in AMI patients.

Objective To verify the significance the Atlas Fixation (AF) for thoracolumbar vertebrae.Methods 61 patients with fracture of thoracolumbar spine were operated by AF internal fixation. And to evaluate the effectiveness of the AF by X ray and CT scans. Results The patients of Frankel's B～D were improved by 1～2 scroe after operation. There were no iatrogenic nerve root injury and broken screws. There no earlier period infection. 2 had peri-screws abscess after 6 months by operation. Conclusion Compared with other spinal posterior internal fixation, AF has better results in effectiveness and simplification.

[Objective]To approach the clinical characteristic,diagnosis and method of treatment of malignant fibrous histiocytoma of bone.[Method]The clinical data of 52 patients of malignant fibrous histiocytoma of bone were analyzed retrospectively,including sites of tumor,preoperative imageology,puncturing biopsy,surgical staging of tumor,methods of treatment,results of follow-up,and so on.[Result]In all 52 cases of malignant fibrous bistiocytoma of bone,32 cases were in periphery of knee joint,14 cases in periphery of shoulder joint,3 cases in the proximal femur,1 case in calcaneus,1 case in lumbar and 1 case in ilium.The preoperative imageelogy and puncturing biopsy were made as usual.There were 20 cases in staging of ⅡA and 32 cases in in staging of ⅡB according to Ennekings surgical staging of tumor.All cases were performed operation except of 1 case.The operation were made salvage limb mosfly and the ratio of limb salvage was 74.5 %(38/51).The preoperative and postoperative chemotherapy and radiotherapy were performed at the same time.Thirty-six of all cases were in long-time follow-up and the ratio of follow-up was 69.2%(36/52).Nine cases were recurred in the region,6 cases had distant metastasis and survival rate of 5 years was 61.1%(22/36).[Conclusion]Preoperative puncturing biopsy and imageology can help the diagnosis of malignant fibrous histiocytoma of bone.Surgery is the primary method and radiotherapy and chemotherapy can prevent the recurrence of the tumor.The resection of the tumor thorough is the key point of healing of malignant fibrous histiocytoma of bone.

Objective To determine the relationship between HBeAg and HBV DNA levels and grading of liver inflammation in patients with chronic hepatitis B. Methods Serum HBeAg and HBV DNA were assayed by MEIA and PCR in 74 patients with HBeAg-negative and 73 patients with HBeAg-positive chronic hepatitis B who had undergone liver biopsy and pathological grading. Results The serum HBV DNA levels in 27 patients(36%) HBeAg-negative chronic hepatitis B were higher 10~5 copies/ml.The number of patient at the HBV DNA level increase along with G1～G4 grading ascendant.The correlation between two factors has statistically remarkable significance.Those serum HBeAg quantitative levels are 0～29 PEIU/ml. The number of HBeAg quantitative-positive patients increase also along with G1～G4 grading ascendant and the correlation between two items has statistically significance.However,there are no relationship between serum HBeAg and HBV DNA and G1～G4 grading in 73 patients with HBeAg-positive chronic hepatitis B whose HBV DNA levels are higher than 10~5 copies/ml. Conclusions Serum HBV DNA level is the indicator by which grading of liver inflammation can be judged in the patients with HBeAg-negative chronic hepatitis B and former is positively interrelated with latter significantly.That Serum HBeAg level is low but HBV DNA duplicate actively may indicate that there is C-zone mutation in 36% patients with HBeAgnegative chronic hepatitis B.Serum HBV DNA level cannot reflect grading of liver inflammation in the patients with HBeAg-positive chronic hepatitis B.

[Objective]To discuss the diagnosis and surgical treatment of spinal myeloma.[Method]To make a retrospective analysis of cliniacal history and operation results of 13 patients with spinal myeloma from 1999 to 2004.There were 5 males and 8 females with average age of 47.67 years.They were diagnosed by X-ray,CT,MRI,ECT and biopsy.Resection of tumor was performed in all cases,of which 7 cases was through anterior approach,4 cases through posterior approach,2 cases through combined approach.[Result]The pain was decreased and neurological function was improved in all cases after the surgery.Three death and one recurrence was found during the follow-up time.[Conclusion]X-ray,CT and MRI are essential for diagnosis,ECT may help found multiple lesions,biopsy is reliable diagnosable method.Surgery can relived the symptoms obviously,but should be associated with adjuvant treatments.

Objectives:To study the colonic adaptation of short bowel rats by enteral nutrition plus fiber treatment.　Methods:The short bowel rats were randomized into two groups:feed by Pepti-2000(EN) and by Pepti-2000 plus fiber(EF);and another normal rats as control(CONT) group.The nutritional states and morphologic changes of the colon were investigated.　Results:From postoperative days(PODs) 15,the weight of EF group was increasing and greater than EN group after PODs 18.The net nitrogen balance in EF group was almost the same as CONT,but greater than EN group.All the mucosal thickness,villous height and surface area of the colon in EF group were greater than CONT and EN group(P<0.001).DNA index and percentage of S phase cells of the colonic mucous in study groups were higher than CONT group(P<0.05).Also the levels of colonic mucous IGF-1 mRNA in study groups were higher than in CONT group(P<0.05).　Conclusions:Enteral nutrition plus fiber can not only promote nutritional states;but enhance colonic adaptation to the short bowel rats.

Objective To investigate the indication of turbine technique or hammer and chisel technique removral of impacted mandibular third molars.Methods 486 impacted mandibular third molars patients were randomly divided into two treatment groups.One group received turbine technigue of 199 cases.while another group received hammer and chisel technique of 287 cases.Both the duration of operation and complications were compared.Results The average duration of the operation with turbine technique was(10 ～ 45)min,while with hammer and chisel technique was(12 ～ 90)min,respectively(P ＜ 0.05)in the removal of impacted mandibalar third molars.there were significant differences between two groups.The standardized rate of complication with tubine tehnique was 28.8％,while with hammer and clisel technique was 55.1％ in the remoral of impacted mandibular third molars.Conclusion Extraction of impacted mandi-bular third molars with air turbine hand piece could reduce complication during operation,and risperidone showed better results.It is worth for clinical use.

The paper proposes and realizes a programmable wireless neural stimulation system which can be used as a solution of functional electrical stimulation to treat neural diseases. The system is composed of two parts: controller and neural stimulator. The controller can transmit pulse parameters to the stimulator wirelessly, and the stimulator can generate bidirectional pulses with charge balance. The simulator takes use of ADCs to sample on the bidirectional pulse output, which compared with preset amplitude to the DAC output voltage to realize the voltage calibration. Through the test, the whole system works stably and the output of the biphasic charge balanced circuit is definite. The stimulator output ranges from 0 to 5 V ajustably, and the frequency ranges from 1 Hz to 200 Hz ajustably, while the pulse width ranges from 500 μs to 1500 μs ajustably. The duration of the stimulation can be set from 10 s to 10 min.
Electric Stimulation ; instrumentation ; Equipment Design ; Wireless Technology

Objective To explore the optimal exercise way of improving the risk indicators of electrocardiogram (ECG) during exercise.Methods Twenty-four healthy male subjects aged between 40 and 49 years old were divided randomly into a control group (n=9),a low amount exercise group of 1200 kcal/wk (n=7) and a high amount exercise group of 2000 kcal/wk (n=8).The intensity of the exercise groups was 65％～ 80％VO2max training in the standard 400 m track for 12 weeks.The accelerometer and polar watch were worn to monitor the exercise process.During the exercise,the exercise amount was controlled using the distance and duration while the intensity was controlled using target heart rate.The subjects took part in the graded exercise test before and after the exercise intervention.Twelve lead electrocardiograms were used to measure the indicators of ST segment depression and corrected QT interval dispersion (QTcd) during the exercise.Results The exercise intervention did not result in significant change in ST segment depression,while the QTcd indicator in both the exercise groups decreased significantly after the intervention (P＜0.05).Moreover,the decrease of QTcd indicator in the low amount exercise group was significantly higher than that of the control group (P＜0.05),while the decrease of QTcd indicator in high volume group was higher than that of the control group (P＜0.01).No significant differences were found in the ST segment depression among the 3 different groups.Conclusion The Twelve-weeks exercise interventions with intensity of 65％ ～ 80％ VO2max and exercise amount of 1200 kcal/wk and 2000 kcal/wk have no effect on the ST segment depression during the exercise.However,they can result in significant decrease in the QTcd of ECG during exercise,reducing the risk of exercise-induced myocardial ischemia.

AIM: To study the anti-aging effects of polysaccharides from rhizomorph of Armillaria mellea(ARP) on fruit fly and mouse. METHODS: Regarding fruit files and D-galactose induced aging mice as research objects,observing the fruit flies lifespan;The ability to learn and memorize on model mice were observed by jumping platform test and maze test,the body weight,thymus and spleen index,SOD and GSH-Px activities、MDA and NO levels in serum,liver and brain were measured. RESULTS: ARP could significantly lengthen the lifespan of fruit flies;Obviously improve the ability to learn and memorize,markedly increase the body weight and resist the atrophy of thymus and spleen of model mice,SOD and GSH-Px activities increased obviously,MDA and NO levels decreased dramatically on model mice. CONCLUSION: ARP has anti-aging effects by immune-regulatory and eliminating free radicals.