To study the protective effect of local mild hypothermia on the ischemic cerebral tissue, a model of middle cerebral artery(MCA) occlusion was prepared in Wistar rats which were randomly divided into the normal temperature group, FK 506 group, mild hypothermia group and control group. The radioactivity of calcineurin in ischemic cerebral tissue was measured in different time after MCA occlusion. The activity of calcineurin decreased 6 hours after ischemia in the normal temperature group and the FK 506 group, whereas no obvious change of calcineurin activity was observed in the mild hypothermia group, which had a significant difference. It is suggested that the local mild hypothermia treatment may inhibit the change of calcineurin activity in ischemic cerebral tissue, and has a tangible protective effect on the ischemic brain.

AIM: This study was designed to investigate the effects of sodium restriction and sodium supplementation on the expression of angiotensinogen mRNA in myocardium of rats with congestive heart failure(CHF).METHODS: Radioimmunassay and in situ hybridization techniques were used to determine the angiotensin Ⅱ(AngⅡ)contents and the expression of angiotensinogen(ANG)mRNA in myocardium, respectively.RESULTS: In sodium restricted group, the plasma sodium was obviously lower than that in CHF group(P

AIM: To investigate the expression of angiotensinogen (ANG)mRNA and the changes of AngⅡ content in rat anterior pituitary during acute stress.METHODS: The expression of ANG mRNA was determined using in situ hybridization technique, and the AngⅡ content were determined by RIA.RESULTS: The ANG mRNA expression and AngⅡ content were significantly increased, and ANG mRNA was increased more significantly than AngⅡ content. CONCLUSION: The local renin-angiotensin system (RAS) in anterior pituitary is obviously activated during stress, which strongly supports the view that local RAS contributes to stress reaction.