AIM: This study was designed to investigate the effects of sodium restriction and sodium supplementation on the expression of angiotensinogen mRNA in myocardium of rats with congestive heart failure(CHF).METHODS: Radioimmunassay and in situ hybridization techniques were used to determine the angiotensin Ⅱ(AngⅡ)contents and the expression of angiotensinogen(ANG)mRNA in myocardium, respectively.RESULTS: In sodium restricted group, the plasma sodium was obviously lower than that in CHF group(P

AIM: To investigate the expression of angiotensinogen (ANG)mRNA and the changes of AngⅡ content in rat anterior pituitary during acute stress.METHODS: The expression of ANG mRNA was determined using in situ hybridization technique, and the AngⅡ content were determined by RIA.RESULTS: The ANG mRNA expression and AngⅡ content were significantly increased, and ANG mRNA was increased more significantly than AngⅡ content. CONCLUSION: The local renin-angiotensin system (RAS) in anterior pituitary is obviously activated during stress, which strongly supports the view that local RAS contributes to stress reaction.