Objective To analyze the MSCT manifestation of tumor of ovary.Methods 57 patients diagnosed as ovary tumor were recruited into our analysis (malignant:33 cases,benign:24 cases),all cases underwent plain CT scan and contrast CT scan.Results 33 cases of malignant tumors showed moderate enhanced.23 ovary adenoma which manifests as cystic tumor,the solid parts of the lesion showed slightly enhanced.Granulosa-theca cell tumor showed slightly enhanced.Conclusion MSCT is helpful in diagnosis of ovary tumor.

Objective To study the clinical effects of aspirin and clopidogrel combined with atorvastatin calcium in patients with acute ischemic cerebrovascular disease. Methods 27 cases of patients with acute ischemic cerebrovascular disease from March 2015 to March 2016 in Hainan general hospital were selected,and randomly divided into control group and observation group,13 patients in the control group,treated with aspirin and clopidogrel,14 cases in the observation group,treat with atorvastatin calcium based on the control group,both two groups were treatment for three months.The total effective rate were compared between the two groups after treatment,NIHSS score and serum lipid level were compared before and after treatment. Results After treatment,the total effective rate of observation group was 92.9%,significantly higher than the control group 53.8%(P<0.05).Compared with before treatmeng,the NIHSS score of two groups were decreased,the levels of TC,TG and LDL-C in two groups were decreased,HDL-C was increased (P<0.05);Compared with control group,the NIHSS score of observation group was lower,TC,TG and LDL-C were lower,HDL-C was higher (P<0.05). Conclusion Aspirin and clopidogrel combined with atorvastatin calcium in treatment with acute ischemic cerebrovascular disease has significant effect,can reduce the patient's NIHSS score,improve blood lipid levels.

Objective:To investigate the serum Vitamin A(sVit A) levels in Behcet′s disease(BD)and other rheumatological diseases.Methods: sVit A concentration was measured in BD(including 3 subtypes of eye BD,intestinal BD,and vascular BD),rheumatoid arthritis(RA),systemic lupus erythematosus(SLE),ankylosing spondylitis(AS),primary Sjogren′s syndrome(pSS) and adult onset Still disease(AOSD)and compared with healthy controls(HC).Results: The patients enrolled in this study was according with the epidemiological features;BD patients including its subtypes of eye BD,intestinal BD,and vascular BD patients all had a lower concentration of sVit A than HC(P<0.05),no statistical differences between the subtypes;RA,SLE,AS,pSS and AOSD had a lower concentration of sVit A than HC(P<0.05).Conclusion: Decreased serum Vit A levels might account for a common pathogenesis of various rheumatic diseases.

The anesthesiology discipline development not only must comply with its own inherent laws of development,but also must focus on social,economical and the whole teaching environment of higher education as well as the discipline ecology evolution composed by neighbor discipline of anesthesiology.Its long term mechanism construction is discussed.

Accidents ; Adolescent ; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors ; genetics ; metabolism ; Carcinoma, Renal Cell ; genetics ; pathology ; surgery ; Chromosomes, Human, X ; Diagnosis, Differential ; Gene Fusion ; Humans ; Kidney ; injuries ; Kidney Neoplasms ; genetics ; pathology ; surgery ; Lymphatic Metastasis ; Male ; Translocation, Genetic

Objective To characterize the alteration in peripheral blood endothelial progenitor cells (EPCs) of patients with systemic lupus erythematosus (SLE).Methods Venous blood samples were obtained from 82 female patients with SLE aged (35 ± 10) years and 50 healthy female controls aged (35 ± 13) years.ACL 9000 automated coagulation analyzer was used to determine the level of Von Willebrand factor antigen (vWF Ag).Flow cytometry was performed to detect peripheral blood EPCs and circulating endothelial cells (CECs).Analysis of variance was performed to assess the differences in these parameters between patients with active and stable SLE and the controls,and Pearson correlation analysis was conducted to evaluate the relationship between these parameters.Results The number of CD34+ cells,CD133+ cells and CD34+CD133+cells per 200 000 peripheral blood mononuclear cells was 35.4 ± 16.7,86.5 ± 32.1 and 361.3 ± 176.4 in patients with active SLE,significantly higher than that in the patients with stable SLE (17.1 ± 10.9,28.7 ± 21.5,107.2 ±44.3,respectively,all P ＜ 0.01)) and the controls (13.8 ± 9.6,11.2 ± 5.5,92.3 ± 50.5,respectively,all P ＜0.01).The patients with active SLE exhibited an elevated level of vWF Ag (438.9％ ± 205.3％ vs.130.2％ ±51.5％,P ＜ 0.01),an increased number of EPCs (361.3 ± 176.4 vs.107.2 ± 44.3,P ＜ 0.01) but a similar number of CECs (127±51 vs.118 ± 39,P ＞ 0.05) per 200 000 peripheral blood mononuclear cells compared with the healthy controls.No significant differences were observed in these parameters between the patients with stable SLE and the controls (all P ＞ 0.05).The number of EPCs was positively correlated with the level of vWF Ag (r =0.67,P ＜ 0.01),but uncorrelated with the number of CECs (P ＞ 0.05) in patients with active SLE.Conclusions The quantity of EPCs in peripheral blood is closely correlated with the level of the vascular injury marker vWF Ag,hinting that the number of EPCs can serve as a useful marker of disease severity.

Objective: To optimize the sample pretreatment and establish an ultra-high-performance liquid chromatography coupled with hybrid quadrupole-orbitrap high-resolution mass spectrometry (UPLC-Q-Orbitrap-MS) assay based on the parallel reaction monitoring (PRM) mode for determination of antibiotic residues in chicken meat. Methods: Blank matrix-spiked chicken meat samples were extracted with 95% acetonitrile aqueous solution containing Na2EDTA and formic acid. The extraction solutions were cleaned up using different combinations of C18, PSA and GCB fillers, and the combinations with a higher antibiotic recovery rate was screened. The residues of 32 antibiotics were determined using UPLC-Q-Orbitrap-MS based on the PRM mode. Results: If the extraction solution was cleaned up using the C18 filler, the largest number of antibiotics with a spiked recovery rate of >80% was seen, with matrix effects of 82.2% to 112.6%. The detection limits of 32 antibiotics were 0.8 to 5.8 μg/kg, with linear correlation coefficients of >0.99, spiked recovery rates of 71.3% to 111.5% and relative standard deviations of 3.2% to 14.2%. Conclusion The UPLC-Q-Orbitrap-MS assay is suitable for determination and quantitative analysis of multiple antibiotics in chicken meat. Key words： high-resolution mass spectrometry orbitrap antibiotic residue parallel reaction monitoring

Objective To evaluate the detection methods for β‐lactamase in 3 kinds of Staphylococcus aureus to provide the basis for clinical laboratories selecting the best detection method .Methods 40 strains of Staphylococcus aureus were selected .With the BlaZ gene detection result as the gold standard for β‐lactamase detection ,the detection performance of the penicillin zone‐edge deter‐mination ,cloverleaf assay ,nitrocefin‐disk were evaluated .Results 20 strains of Staphylococcus aureus were the BlaZ gene positive detected by PCR and 20 strains were negative .The sensitivity of the penicillin zone‐edge determination method ,cloverleaf assay and nitrocefin‐disk were all 100 .00% and the specificity were 100 .00% .Conclusion Three kinds of β‐lactamase detection method have better sensitivity and specificity .So different laboratory can choose one of the method to detect β‐lactamase of staphylococcus aureus according to the actual situation .

Volume concentration determination for atmospheric krypton and xenon is very important for krypton-85 and radioactive xenon isotopes monitoring. An injection setup integrated adjustable quantity sample injection and quantitative dilution function was designed. The effects of EI source parameters on the sensitivity of MS detector were studied. The optimized values were as following: ionization energy of 70 eV, emission current of 40 mA, cathode voltage of 27 mV, focus voltage of 85 mV and lens compensation of 20 V, respectively. A GC-MS method for the determination of krypton and xenon in atmosphere without of sample pretreatment was developed. The minimal detected concentrations for krypton and xenon were 3. 3×10-8(V/V) and 2. 6×10-9(V/V). Moreover, the krypton and xenon concentrations in the ground level air around our laboratory were measured with the results of 1 . 1 × 10-6 ( V/V ) and 9 . 3 × 10-8 ( V/V ) . The related combined standard uncertainties for krypton and xenon results were 2. 38% and 3. 15%, respectively.

Objective To study the effect of lipoteichoic acid(LTA) of Bifidobacterium on the expression of vascular endothelial growth factor(VEGF) and invasion and metastasis of colon carcinoma cell lines.Methods The effects of LTA on adhesion,invasion and metastasis of human colon carcinoma lines LoVo and HT-29 in vitro were examined by MTT colorimetric and transwell chamber.The mRNA and protein expressions of VEGF in the two colon carcinoma cells treated with LTA were detected by RT-PCR and Western blot.Results The adhesion ability decreased in LoVo cells and HT-29 cells after the treatment of LTA(20,50,80 ?g/ml) for 30,60,90,120 min(P