Exosome are nanoparticles released by various cells into extracellular environment, with the diameter of 40-100 nm.Exosome contain different kinds of biological materials, such as proteins, mRNA, microRNA.Exosome can not only mediate cell-to-cell communication, but also be involved in many physiological and pathological processes such as immune response regulation, inflammation, tumorigenesis and drug resistance.This article mainly focuses on correlation between exosome and drug resistance of rumor.

Cystatin C is widely present in nucleated cells of various tissues and body fluids as a cysteine protease inhibitor.It can predict the occurrence of diabetes,and it was significantly associated with diabetic macrovascular and microvascular complications such as albuminuria,retinopathy,peripheral arterial disease,and coronary artery disease.This review introduces the research progress of cystatin C in diabetes and diabetic vascular complications.

Objective To discuss on the effect of simulation training of vocational protection among nurses with lower age and services seniority in trauma surgery department. Methods The training of vocational protection was divided into the curricula content and simulation training, 37 nurses were trained with the method of combining theory with practice,the items before and after the training were tested with t test. Results The mastering degree of occupational exposure knowledge after the training significantly improved compared with that before training. After simulation training various protection technique greatly alleviated. Each score of comprehensive clinical evaluation after the training also increased. Conclusions We should strengthen occupational protection in clinical work in order to prevent nosocomial infection.Detail education and situational education should be paid attention to during the training according to their ability,so that satisfactory results can be achieved.

Objective To investigate the changes of serum myocardial enzyme in schizophrenia patients spectrum and its clinical significance.Methods 405 patients with schizophrenia hospitalized patients serum myocar-dial enzyme (CK,CK-MB,LDH,α-HBDH level,) on admission and 4 weeks after treatment the serum myocardial enzyme level,analyze its relationship with the disease, and 100 healthy people ( control group) were compared. Results the positive symptoms of schizophrenia patients before treatment,serum CK,CK-MB,LDH,α-HBDH were (1 286.52 ±714.38) U/L,(40.72 ±27.38) U/L,(143.06 ±33.24) U/L,(115.75 ±22.74) U/L,CK,CK-MB were higher than those in the control group (t=10.35,9.29,all P<0.01);after 4 weeks of treatment,CK,CK-MB, LDH,α-HBDH were (98.13 ±38.75)U/L,(12.87 ±5.73)U/L,(125.43 ±25.74)U/L,(102.15 ±26.86)U/L, compared with the control group,the differences were not statistically significant(all P>0.05).Conclusion Serum myocardial enzyme CK and CK-MB increased level was related with diseasein the patients with acute exacerbation of schizophrenia.

Objectives To study the relationship between hope and quality of life(QOL)in nasopharyngeal carcinoma(NPC) patients undergoing radiotherapy(RT). Methods One hundred and ten NPC patients undergoing radiotherapy participated in the investigation by the Herth Hope Index(HHI)and the Functional Assessment of Cancer Therapy-head and neck scale(FACT-H&N),to look into the correlations between their hope and QOL.Result The scores on hope and NPC for the QOL patients during radiotherapy were(34.80±2.64)and(91.34±13.19),respectively and they were positively correlated(r=0.253,P<0.01). Conclusions The hope of the NPC patients is at the medium level.Their hope is positively correlated with QOL.The higher level the hope is,the higher the QOL.

ConclusionBushen-Yitalpowder adjuvant to progesterone therapy can improve the clinical outcome in patients with early threatened abortion.

Objective To assess the prognostic values of the immunohistochemical expression of vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor-2 (VEGFR-2) in a cohort of patients with operable advanced laryngeal cancer who have been treated with induction chemotherapy. Methods VEGF and VEGFR-2 expression in the Forty-nine patients was quantified by an enzyme immunosorbent assay in a retrospective series.Results The moderate to high VEGF expression in 34 patients were found and low expression in 15 patients. VEGFR-2 expression was moderate to high in 31 patients and was low expression in 18. The probability of a complete response to induction chemotherapy was significantly higher in patients with none to low VEGF expression.Conclusion VEGF expression seems to be a significant predictor of complete response to induction chemotherapy.

The student majoring in Medical Biological Technique will be mainly engaged in the practical work of biological technique industry after graduation in the future.In order to bind the theories on the practical biological techniques in designs of contents and roundly improve students' practical ability in classes as well as enriching the communication among students,our college offers an immunologic techniques experiment classes with 36 hours per semester,which has also undergone a reasonable project teaching innovation. This proved to result in a satisfactory outcome in improving the students' practical a

Objective To suty the MRI manifestations of juvenile acute articular cartilage injury of the knee joint.Methods MRI findings of cartilage,subcartilage low signal line and subcarilage bone were analysed retrospectively in 53 juvenile patients (ranged in age from 4~27 years) with acute articular cartilage injury confirmed by arthroscopy.Results Sixty-nine cartilage injuries were showed by MRI in 53 patients,including patellas in 25,femoral lateral condyles in 22,femoral medial condyles in 11,trochlea of the femur in 2,and tibial plateau in 9.Acute articular cartilage injury appeared as pure cartilage fracture in 46, including complete split of the cartilage in 22 sites,partly split of the cartilage in 20 sites,and fissur-like fracture in 4 sites.Osteochondral fracture were observed in 23 sites,including avulsion fracture in 13 and osteochondral subsided in 10.Articular cartilage loose bodies and osteochondral loose bodies were found by MRI in 6 and 13,respectively.Conclusion MRI is the best non-invasive method for studying cartilage injury.

Objective To understand awareness of basic concepts, diagnosis, treatment and patient education for chronic obstructive pulmonary disease (COPD) among practitioners in rural new cooperative medical scheme (NCMS) and evaluate effectiveness of training for them in Haiyang, Shandong.Methods In total, 116 practitioners, two or three randomly selected from each of 43 NCMS service stations under the Third People's Hospital of Haiyang, were surveyed with questionnaire and then systematic training was provided for them. Another survey was conducted among them a half and two years after the first one with the same questionnaire. Results At the first survey, only 9.5 % (11/116) of the practitioners surveyed knew about "Guidelines for Prevention and Control of Chronic Obstructive Pulmonary Disease",19. 8 % (23/116) knew clinical characteristics of COPD, 29. 3 % (34/116) knew that pulmonary function test is gold standard for COPD diagnosis, 62. 9 % (73/116) knew that smoking is major inductive factor for COPD and could persuade patients to quit smoking, only 2.6 % (3/116) could make registration for the patients and provide health education for them, 48. 3 % (56/116) knew that the patients should be immunized with influenza vaccine, and 7. 8 % (9/116) knew long-term oxygen therapy for the patients. At the second survey, 94. 8 % (110/116) of the practitioners surveyed knew about " Guidelines for Prevention and Control of Chronic Obstructive Pulmonary Disease", all of them knew characteristics of COPD, that pulmonary function test is gold standard for COPD diagnosis, smoking is major inductive factor for COPD,could make registration for COPD patients and provide health education for them, 99. 1% (115/116) used influenza vaccine for COPD patients, and 99. 1% (115/116) knew long-term oxygen therapy for the patients. Conclusions Systematic training for COPD knowledge among practitioners in rural NCMS seems to be significantly beneficial for their awareness about the illness and to improve their abilities of prevention and treatment for it in rural areas.