1.The therapeutic effects of UPASS-Ⅱ percutaneous pedicle screws internal fixation treating thoracolumbar fracture
Jianlan WAN ; Yuyong YANG ; Yiliang PENG
Chongqing Medicine 2013;(30):3638-3640
Objective To observe the therapeutic effects of UPASS-Ⅱ minimally invasive spinal system percutaneous pedicle screws internal fixation treating thoracolumbar fracture .Methods From May 2011 to December 2012 ,26 patients(observing group) were sufferred from thoracolumbar fracture without neural impairment were treated with UPASS-Ⅱ minimally invasive spinal sys-tem percutaneous pedicle screws internal fixation ,and were compared with other 26 cases(control group) adopted the treatment of conventional open pedicle screws internal fixation in the same period .Results All patients were followed up more than 6 months (mean 9 .2 months) ,the comparison of Cobb′s angle ,anterior vertebra height ,VAS ,JOA score and ODI between pre and post oper-ation were all significantly different in both group(P<0 .01) ,but the operation time and length of stay in the hospital in observing group were significantly shorter than that in control group (P<0 .05) ,moreover ,the amount of operative bleeding and drainage of post operation in observing group decreased obviously than that in control group (P<0 .01) .Conclusion This study shows that UPASS-Ⅱ minimally invasive spinal system percutaneous pedicle screw internal fixation treating thoraco-lumbar fracture is a safe and effective treatment strategy in selected patients .
2.Study on ultrasonic circulation technique to extraction of triterpenoids from Ganoderma lucidum
Shuming HUANG ; Xinlin YANG ; Ziqiang ZHANG ; Jianlan XU ; Hesun ZHU
Chinese Traditional and Herbal Drugs 1994;0(05):-
ObjectTo study the application of ultrasonic circulation technique for the extraction of triterpenoids from Ganoderma lucidum (Leyss.ex Fr.) Karst. Methods On the basis of conventional method, the processing steps of ultrasonic circulation treatment were added. Results The comparative experiments showed that less amounts of various solvents and shorter extraction time were needed for ultrasonic circulation extraction, with the product ratio of about 40 percent higher than that of conventional method. Furthermore, a good consistency of the target product analyzed by HPLC was found between two different extraction methods. Conclusion The ultrasonic circulation technique has a potential application to the extraction of triterpenoids from G. lucidum.
3.The Inhibitory Effect of OGT Gene Expression on The Level of tau Phosphorylation
Jiangyong YANG ; Jianlan GU ; Jianhua SHI ; Fei LIU ; Qin SHEN
Progress in Biochemistry and Biophysics 2006;0(03):-
To investigate the effect of OGT expression inhibited by RNAi on the alteration of tau phosphorylation and glycosylation level in HEK293T cells.The siRNAs targeting OGTgene(OGT-siRNA1~3) were designed and chemically synthesized.The OGT-siRNA1~3 were transfected into HEK293T cells via lipofectamine2000.The efficacy of RNA interference was detected by RT-PCR.The fluorescence of GFP/OGT was counted by fluorescence microscopy after pEGFP/OGT and OGT-siRNA1~3 cotransfected to HEK293T cells for 24 h.The level of tau phosphorylation and glycosylation were detected by Western blot after Plasmid pCI/tau441 and the siRNA cotransfected HEK293T cells for 48 h.OGT-siRNA3(100 nmol/L) could effectively downregulate the expression of OGT gene compared with Mock group(80.0% at mRNA level and 51.3% at protein level).The level of phosphorylation at various sites of the tau was significantly upregulated and the glycosylation was downregulated following the inhibition of OGT gene expression.There is an apparent negative correlation between the modification of phosphorylation and glycosylation of tau protein, the deficient in glucose uptaken/metabolism may be an important pathogenesis of AD.
4.Significance of WT1 gene expression in patients with myelodysplastic syndromes
Jianlan LI ; Hongwei WANG ; Xiaohong LI ; Bo YANG ; Lei ZHU ; Yanhong QIN
Journal of Leukemia & Lymphoma 2009;18(2):104-106
Objective To determine whether pattern of WT1 gene expression is a useful marker for establishing prognosis and tracking disease progression in patients with myelodysplastic syndromes (MDS). Methods RNA were extracted from bone marrow smears of 45 patients with MDS, the WT1 expression were tested by FQ-RT-PCR. Results The degree of WT1 expression was increased during disease progression of MDS. The WT1 expression level was increased more a logarithm grade when a RA patient developed into RAEB, the fluctuation of WT1 expression level was decreased a logarithm grade during RA. Conclusion WT1 gene is a useful marker for assessment in MDS patients. It pointed out may diagnosis RA if the WT1 expression level higher two logarithm grade than the mean of normal control for patients which didn't diagnose by morphology. It may suggest disease progress of MDS if the WT1 expression level increased more a logarithm grade recently. Further confirmation was needed because the case was limited.
5.Optimization of Extraction Technology of Paeonol from Paeonia Suffruticosa
Dandan WANG ; Jun CHEN ; Junli LI ; Shi YANG ; Jianlan TANG ; Gang DING ; Wei XIAO
World Science and Technology-Modernization of Traditional Chinese Medicine 2013;(9):1998-2001
This article was aimed to study the optimum extraction process of paeonol. The extraction yield of paeonol was taken as investigation index. And the best extraction process was screened by orthogonal experimental design. The results showed that the optimum condition of extraction process was to soak coarse powder of Paeonia suffruti-cosa into 15-fold water for 0.5 h, and then the distillation lasted for 2.5 h. The distillate was collected and cooled to room temperature. The crystallization lasted for 24 h at 4℃, and then filtered and dried for 48 h at room tempera-ture. It was concluded that the selected technology was stable, reasonable and feasible. The extraction yield of paeonol is over 80%.
6.Current implementation of informed consent among counselors in Beijing
Haoyu WANG ; Mengke GOU ; Mingyi QIAN ; Wenting SUN ; Shujie ZHUANG ; Jingjing YANG ; Tianyue MI ; Tianshu LIU ; Jianlan YANG
Chinese Mental Health Journal 2017;31(1):58-63
Objects:To investigate the current situation of counselors'implementation and their attitude to informed consent in Beijing.Methods:Eleven counselors who worked in Beijing were interviewed,the average of their working years was (7.4 ± 4.4).A semi-structured interview was used to learn about their practicing processes in and viewpoints on informed consent,and their interview transcripts were analyzed through qualitative method.Results:The results could be categorized into four categories,including the content of informed consent,the influential factors of informed consent,problems and confusions,and the significance of informed consent.The crucial role of informed consent in psychotherapy was generally recognized by counselors,and they could obey the related rules as well.The therapists had some confusion and problems about informed consent,such as the form and length,the consent for special population and online consultation.Conclusion:Basically,the counselors in Beijing could comply with the ethical standards of informed consent.However,they also suggest that certain limitations of these rules should be noticed.
7.Performance evaluation of community continuous rehabilitation intervention on patients with stroke
Yongli WANG ; Xueping DU ; Jianqin DONG ; Lan DING ; Jianlan WANG ; Qingying HENG ; Yingyu JIA ; Xiaoying LIU ; Yuling LIU ; Yuanyuan YANG
Chinese Journal of Practical Nursing 2008;24(30):1-4
Objective To explore the pathway of continuous rehabilitation intervention in community stroke patients and to evaluate its effect. Methods Stroke patients (64 cases) were selected from Yuetan community as the rehabilitation group by objective sampling based on inclusive and exclusive standard and 64 stroke patients wre selected from other community in Xicheng district as the control group. At the same time, 16 doctors and nurses from 10 clinics of Yuetan community health service center were trained and they gave reha-bilitative intervention to the rehabilitation group. After intervention for 6 months, the effect of rehabilitative in-tervention was evaluated compared with the control group who did not receive rehabilitative intervention. Re-suits The Fugl-Meyer score of the rehabilitation group was (76.14±12.48), the score of the control group was (19.36±14.32),which had significant difference compared with the rehabilitation group, P < 0.05. The Batthel index of the rehabilitation group was (72.25±10.22), the index of the control group was (22.62±9.71),which was significantly different from that of the rehabilitation group, P < 0.05.Besidcs, the rate of knowing health knowledge and healthy exercise in the rehabilitation group was higher than that of the control group, P < 0.05. Condusions The rehabilitation information net and systemic community rehabilitation intervention can im-prove the motor ability and the ability of daily activity for patients with stroke.
8. Clinical and laboratory characteristics of chronic neutrophilic leukemia: analyses of five cases
Sufen GUO ; Yan HUANG ; Jianlan LI ; Bo YANG ; Xiuhua CHEN
Journal of Leukemia & Lymphoma 2018;27(4):234-237
Objective:
To investigate the clinical characteristics, cell morphology, genetics, gene mutations of the patients with chronic neutrophilic leukemia (CNL).
Methods:
Five CNL patients from the Second Hospital of Shanxi Medical University between May 2011 and May 2017 who conformed to 2016 World Health Organization (WHO) diagnostic criteria were retrospectively analyzed from clinical characteristics, laboratory features and treatment methods.
Results:
The peripheral blood white blood cell count (WBC) of 5 CNL patients was significantly increased, and the average WBC was 81.26×109/L [(29-217)×109/L]. Morphological analysis of peripheral blood cell showed a sustained increasing number of matured neutrophilia (0.80-0.85). Neutrophil alkaline phosphatase (NAP) activity was increased (144-266). Bone marrow cell morphology typically showed granulocyte proliferation without obvious dysplasia. Gene detection showed 3 patients with CSF3R T618I mutation and 2 patients with JAK2 V617F mutation in 5 WHO-defined CNL patients. Bone marrow biopsy with reticular staining showed that marrow fibrosis (MF) degree in patients with JAK2 V617F mutation (MF≥2) was higher than that in patients with CSF3R T618I mutation(MF<2).
Conclusions
CNL is a rare type of chronic leukemia, and CSF3R T618I mutation is a specific diagnostic index for CNL. JAK2 V617F mutations alone may be related to myelofibrosis, which remains to be further studied.
9.Double watermarking algorithm for CT and MRI images based on contourlet transform
Zhenyu TIAN ; Hao XU ; Xinxin CUI ; Jing LIU ; Jianlan YANG
Chinese Journal of Medical Physics 2023;40(12):1523-1530
Objective To propose a double watermarking algorithm based on contourlet transform and speckle detection to automatically select the region of interest(ROI)for solving the problems of copyright infringement and malicious destruction of CT and MRI images.Methods The method used speckle detection and singular value decomposition to select the most relevant location in the low frequency domain of the medical image contourlet wave as the ROI,generated a zero-watermarked image in ROI for protecting image integrity,and embed the ROI zero-watermark and the copyright watermark together in the region of non-interest.Results Compared with other double watermarking algorithms,the double watermarking algorithm could greatly improve the invisibility of the carrier image after the attack.The double watermarked images had a structure similarity index measure higher than 94%.Conclusion The proposed double watermarking algorithm incorporating speckle detection and contourlet transform has better robustness against conventional attacks and geometric attacks,and significantly improves the visual quality of carrier images.
10.Application value of dynamic monitoring of serum soluble interleukin-2 receptor and stromal cell-derived factor 1α in the evaluation of patients with acute leukemia
Jianlan LI ; Ju QIAO ; Ying YANG ; Lijuan XU ; Zhipeng LIANG ; Hongfang JIA
Journal of Leukemia & Lymphoma 2020;29(4):232-235
Objective:To explore the application value of dynamic monitoring of serum soluble interleukin-2 receptor (sIL-2R) and stromal cell-derived factor 1 α (SDF-1α) in patients with acute leukemia (AL).Methods:A total of 187 patients with AL admitted to the Second Hospital of Shanxi Medical University from March 2017 to August 2018 were selected and 90 healthy subjects at the same period were selected as the healthy controls. The levels of serum sIL-2R and SDF-1α were detected at the initial diagnosis, remission phase and relapse phase, respectively. The clinical value of dynamic monitoring of sIL-2R and SDF-1α was analyzed.Results:Among 187 patients with AL, 135 patients (72.19%) had complete remission (CR) after chemotherapy, 52 patients (27.81%) had partial remission, and 43 patients (31.85%) relapsed. The level of serum sIL-2R at the AL initial diagnosis period was (533±32) U/ml, which was higher than that in the healthy controls [(247±30) U/ml], and the difference was statistically significant ( t = 71.976, P < 0.01); the level of serum SDF-1α at the AL initial diagnosis was (2 968±305) pg/ml, which was higher than that in the healthy controls [(1 358±160) pg/ml], and the difference was statistically significant ( t = 47.043, P < 0.01). The levels of serum sIL-2R [(308±30) U/ml] and SDF-1α [(1 576±184) pg/ml] in the CR phase were lower than those in the initial diagnosis of patients with AL; and the levels of sIL-2R [(599±36) U/ml] and SDF-1α [(2 894±301) pg/ml] in the relapse phase were higher than those in the CR phase of patients with AL (all P < 0.01). Conclusions:The levels of serum sIL-2R and SDF-1α in patients with AL are increased, and there are big differences in the levels of sIL-2R and SDF-1α at the initial diagnosis, remission phase, and relapse phase. Dynamic monitoring of both can provide the data support for early clinical intervention.