Objective To investigate the role of cystatin C in hypertensive disorder complicating pregnancy.Methods Peripheral blood from 39 healthy pregnant women,23 patients with mild gestational hypertension ,25 patients with moderate gestational hypertension and 27 patients with severe gestational hypertension,CysC,BUN,UA and crea were determined .Resultss CysC ,BUN,UA and crea were higher in gestational hypertension group. CysC ,BUN,UA and crea were higher in severe gestational hypertension than mild and moderate gestational hypertension group.CysC had higher positive predictive ratio than UA for severe gestational hypertension.Conclusion CysC was a sensitive index to detect the early defection of kidney in severe gestational hypertension combining with other index such as BUN,UA and crea.

Objectives To localize the initiation site of the transcript driven by the cDNA corresponding to SARS - CoV 5 ' - UTR when it act as a promoter. Methods The plasmid pGI3 -5' - UTR, in which a reporter gene Luc was driven by the cDNA corresponding to SARS - CoV 5' -UTR, was transfected into HepG2 cells, and then the total RNA was extracted. Subsequently, the extracted total RNA was reverse - transcribed using a specific primer and subjected to two turns of semi - nested PCR. The PCR product was cloned into a T - vector for sequencing. Results A 440bps product was harvested by reverse - transcription and two turns of semi - nested PCR, and the 56th nucleotide of SARS - CoV 5 ' - UTR was found being the initiation site, followed downstream with a highly conserved transcription - regulating sequence (TRS). Conclusion The 56th nucleotide and its downstream TRS of SARS - CoV 5 ' - UTR is key roles in regulating gene transcription.

Objective To investigate the efficacy of Xuebijing injection on the septic shock and its impact on the patient's PCT and CRP.Methods According to the digital table,60 patients with septic shock were divided into two groups according to random number table methods.30 patients in the control group were only given conventional treatment,while the research group were used of Xuebijing injection in addition.The clinical efficacy was compared after treatment and the PCT and CRP levels were detected before and 7d after treatment.The days of hosoitalization were recorded.Results The total effective rate of the study group was 70％,significantly higher than 40％ of the control group's (x2 =5.45,P ＜ 0.05),and the study group's PCT and C RP levels were significantly lower than before (t =33.54,48.02,all P ＜ 0.05),after treatment 7d and the extent significantly greater than the control group (t =6.68,7.67,all P ＜ 0.05).Furthermore,the number of days of study group's hospitalization was significantly lower than that of control group(t =10.21,P ＜ 0.05).Conclusion Septic shock treated by Xuebijing injection has a significant effect as well as a greater impact on the PCT and CRP,which is worthy of clinical use.

OBJECTIVE To find out the situation of peri-operational lower respiratory tract infection in inpatients with congenital heart disease of left to right shunt and provide evidence for clinical prevention and treatment.METHODS Peri-operational lower respiratory tract nosocomial infection in 2 564 cases of congenital heart disease with left to right shunt during Jan 2003 to Dec 2005 was analyzed retrospectively.RESULTS The incidence rate of lower respiratory tract infection was 11.27% among 2 564 cases.The incidence rate was 15.51% in patients less than 2 years old.The most common pathogen was Pseudomonas aeruginosa(18.60%).The death rate in cases with lower respiratory tract nosocomial infection was higher than these non-infection cases.CONCLUSIONS Pulmonary congestion,less than 2 years old,mechanical ventilation and antibiotics abuse are main causes of lower respiratory tract nosocomial infection in patients with congenital heart disease with left to right shunt.Nosocomial infection can increase death rate.

Based on the report of previous clinical study which showed cholinergic receptor antagonist scopolamine had antidepressant activity, this study was to investigate the antidepressant activity of scopolamine and explore its effective dose in mice, and to evaluate the effect of scopolamine on the central nervous system and learning/memory ability at its antidepressant effective dose. Tail suspension test, forced swimming test, step-down passive avoidance test and open field test were used to evaluate its effects on mice. Compared with the vehicle control group, single-dose administration of scopolamine (0.1-0.4 mg x kg(-1), ip) significantly decreased the immobility time (P < 0.01 or P < 0.001) in tail suspension test, and significantly decreased the immobility time (P < 0.001) in forced swimming test, but had no effect on the step-down latency and errors in step-down passive avoidance test. Scopolamine (0.1 and 0.2 mg x kg(-1), ip) had no influence on the locomotor activity in open field test, while at dose of 0.4 mg x kg(-1) significantly increase the locomotor activity. These results showed that scopolamine produced reliable antidepressant effect at doses of 0.1 and 0.2 mg x kg(-1), without impairment on learning and memory, as well as excitory or inhibitory effect on central nervous system in mice.

Aiming at the problems of cosmetic elective courses teaching,this article discusses some effective teaching methods such as adjusting teaching contents according to students'interest and putting comprehensive examination and evaluation into practice through optimizing teaching means.These measures have improved the teaching quality and effect of the elective course and meanwhile are beneficial attempts for the quality education

Objective To observe effects of BMP-4 gene combined with TGF-β1 in repairing rabbit femur defect resulted from firearm wound. Methods The femur defect was made with firearm steel ball. Plasmid encoded BMP-4 gene identified in vitro and TGF-β1 were injected into the tissue of upper, lower and central parts of the defects at the second week after wound. The mRNA and protein expressions of BMP-4 in vivo were detected by real time-polymerase chain reaction (RT-PCR) and Western blot. Activity of alkaline phosphatase (ALP) and calcium content were measured for evaluating osteogenetic ability. The process and quality of osteogenesis were determined by pathological and X-ray examinations. Results mRNA and protein of BMP-4 could continually express for six weeks in vivo after injection. Activity of ALP in the experimental group was increased to ( 13.17 ±0.51 ) U/100 ml at the 8th week, which was significantly higher than (8.77 ± 0.44) U/100 ml in the control group, indicating that osteogenetic ability was markedly enhanced, which accorded with determination of calcium content. Pathological observation and X-ray proved prominent improvement of osteogenesis, with a shorter time and better quality, in the experimental group. Conclusion BMP-4/TGF-β1 can promote repair of firearm femur defect.

Objective:To study the mechanisms of chronic injury in heart allograft with chronic rejection.Methods:A model of cardiac chronic rejection in the rat based on tolerance induced through donor specific blood transfusions was used.The subpopulation and the distribution of infiltrating cells and the expression of adhesion molecule and growth factors were studied by immunohistochemistry and Northern blot analysis in the chronic rejection model.Results:Infiltrating cells were predominantly T lymphocytes and macrophages,mostly evident in the interstitial,subendocardial and perivascular areas.Allografts were demonstrated significantly elevated number of CD45+,CD4+,CD8+,TCR+,CD45RB+ cells,macrophages(ED_1) and NK cells at both 3 and 6 months compared with normal rat hearts and isografts at 3 months.Damaged areas also were observed expression of the adhesion molecules ICAM-1,LFA-1,basic fibroblast growth factor (bFGF) and transforming growth factor(TGF-?1).Northern blot analysis of total RNA,showed 5 to 6 fold upregulation of TGF-?1 mRNA in the chronic rejection model compared with normal rat hearts.Conclusion:These results suggest that T lymphocytes and macrophages play a central role in the development on chronic rejection and the increased expression of ICAM-1,LFA-1,bFGF and TGF-?1 in this model supports the involvement of these adhesion molecules and growth factors in the development of cardiac allograft vasculopaphy.

Objective To explore the protein expressions of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1), and to investigate their relationships between their serum concentration before operation and the infiltration and metastasis of thyroid carcinoma. Methods The protein expressions of MMP-9 and TIMP-1 in 32 cases of thyroid carcinomas, 23 cases of adjacent tissues and 30 cases of benign hyperplastic lesions were measured by using immunohistochemistry. The preoperative serum concentrations of MMP-9 and TIMP-1 in 21 cases of thyroid carcinomas and 19 cases of benign hyperplastic lesions were determined by enzyme-linked immunosorbent assay. Results The positive expression rates of MMP-9 and TIMP-1 in tumor tissues were significantly higher (75.0%,56.3%)than those in adjacent tissues and benign hyperplastic lesions (30.4%, 21.7%; 26.7%, 23.3%) P

Dioxin-like compounds are a class of persistent organic pollutants(POPs).Because of their high toxicity, stability and bioaccumulation, they have serious effects on human health and environment.Studies on the toxicity of dioxins have involved in many aspects, such as reproduction toxicity, immune toxicity, carcinogenicity, endocrine toxicity, and so on.However, few studies of the effects on intelligence had been done,and its potential mechanism had not been reported till now.In this review, the authors tried to generalize the relationship between dioxins and intelligence based on animal behavioral tests and population investigations and hypothesize its potential mechanisms.