1.Effect of Mometasone Furoate Nasal Sprays Combined with Montelukast on Allergic Rhinitis
China Pharmacist 2014;(11):1910-1911
Objective: To observe the curative effect and reoccurrence prevention of mometasone furoate nasal sprays combined with montelukast on allergic rhinitis ( AR) . Methods:Totally 74 cases of patients with AR were divided into the observation group and the control at random. The patients in the observation group were given mometasone furoate nasal sprays combined with montelukast, while the patients in the control group were only given mometasone furoate nasal sprays for 4 weeks. The clinical curative effect and ad-verse drug reaction ( ADR) in the two groups were observed and compared, and the reoccurrence rates in the two groups after one-year following-up were compared as well. Results:The total efficiency in the observation group was 94. 59%, which was much higher than that in the control group (78. 38%), and the reoccurrence rate in one year was 22. 86% in the observation group, which was much lower than that in the control group (48. 28%) with statistical differences (P<0. 05). After comparing the occurrence rate of ADR in the two groups, the difference was not statistically significant (P >0.05). Conclusion: Compared with mometasone furoate nasal sprays, the application of mometasone furoate nasal sprays combined with montelukast in the treatment of AR can reduce the reoccur-rence rate with good security.
2.Expressive variety of choline acetyltransferase in different cerebral regions of rats with fimbria/fornix transection
Zongjun GUO ; Liying JIN ; Jianjun YIN
Chinese Journal of Tissue Engineering Research 2006;10(2):176-178
BACKGROUND: The choline acetyltransferase (CHAT) is the key synthetic enzyme for acetylcholine, and is the important symbol of the functional activity of the cholinergic system. There was a relationship of the cholinergic neurons damage with the pathogenesis of Alzheimer disease (AD) and the mild cognitive impairment (MCI). Whether or not the fimbria/fornix transection may affect the expressive variety of ChAT in different cerebral regions of rats (cortex, hippocammpi CA1, amygdala, Meynert nucleus) is very important for the recognition of the pathogenesis of AD and MCI and the establishment of experimental animal model of AD.OBJECTIVE: To observe the expressive variety of ChAT in different cerebral regions of rats with fimbria/fornix transection and discuss exploratorily the methods of simulative experimental AD.DESIGN: A randomized and control study.SETTING: Institute of Cerebrovascular Disease, Affiliated Hospital of the Qingdao University Medical College and the Department of Neurology, No. 1People' s Hospital of Jining.MATERIALS: The experiment was completed in the Institute of Cerebrovascular Disease, Affiliated Hospital of the Qingdao University Medical College from March to December 2003. Totally 14 adult healthy female Wistar rats aged 5 months were randomly divided into model group and control group with 7 in each group.METHODS: ① The bilateral fimbria-fornix of brain in model group were transected on the stereotaxic apparatus to set animal model. After opening the skull, at the coordinates, along the bregma posterior 2.2 mm-2.5 mm and lateral 1.0 mm according to the atlas of Paxinos and Watson, and the dorsal fornix, the anterior part of hippocampus and the fimbria were cut off with a double blade under visual inspection. Rats in the control group were not performed with fimbria/fornix transection, and the other procedures were completed as those in the model group. ② On the 28 day after the surgery, all rats were killed under anesthesia to take out the brain tissues and make coronary sections for histochemical observation in a immunohistochemical way. The expressions of the ChAT positive neurons were observed in the cortex, hippocammpi CA1, amygdala, Meynert nucleus, and the brown neurons under microscopy was the ChAT positive neurons.MAIN OUTCOME MEASURES: Expressions of ChAT positive neurons in the cortex, hippocammpi CA1, amygdala, Meynert of the basal forebrain in the model group and the control group.RESULTS: All the rats entered the final analysis without any loss. The expression of ChAT positive neurons in the cortex, hippocammpi CA1,amygdala, Meynert of the basal forebrain in the model group were distinctly decreased than that in the control group (2.97±1.45, 32.60±7.33, t=10.51,P < 0.01); (6.83±2.41, 50.57±5.85, t=1 8.30, P< 0.01); (14.43±6.75, 35.43±10.49,t=4.47, P < 0.01); (5.77±6.62, 48.77±7.10, t=1 1.72, P < 0.01), and the differences were significant.CONCLUSION: Finbria/fomix transection can decrease the expression of ChAT positive neurons in many cerebral regions of rats, and can be used in a method of setting the experimental animal model of AD.
3.The effect of Feixian Fang treating the idiopathic pulmonary fibrosis patients in MMPs and TIMPs
Xiaomei ZHANG ; Liangduo JIANG ; Ting YIN ; Wei ZHANG ; Jianjun WU
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(06):-
Objective:To research the therapeutic effect dnd pulmonary ground substance rebuilding in the treatment on IPF patients by Feixian-Fang and.Methods:Treatment 20 cases IPF by Feixian Fang RCT prednisone three months.obsive The therapeutic efficacy and the levels of matrix met alloproteinase 1,2,tissue inhibtor of met alloproteinase(TIMP-1,2).Results: The total effective rate of Feixian-Fang group is 84.2%.The total effective rate of prednisone group is 81.3%.Feixian-Fang can reduce the level of MMP-1,MMP-2,TIMP-1,TIMP-2 in the serum.Concluds:Feixian-Fang can treat IPF probably through inhibiting pulmonary ground substance rebuilding.
4.Effect of blocking the signal pathway of TGFβ on presenting antigen of dendritic cells
Jianjun QIN ; Qinghua ZHOU ; Cheng YI ; Yin LI ; Baoxing LIU
Cancer Research and Clinic 2011;23(10):653-656,660
Objective To explore the dysfunction of dendritic cells (DC) related to TGFβ reversed after blocking the TGFβ signal pathway by recombinant adenovirus vector encoding for Smad7.Methods Smad7 by recombinant adenovirus vector was transfected into dendritic cells.Expression of immunologic phenotypes was detected by FCM,and CTL activity induced by DC was compared.Results The DC modified with Smad7 still expressed high adhesiveness factor related to maturation even if existing exogenous TGFβ1,which was significant statistically compared with DC transfected with control adenoviral vector (P <0.01).Even if existing exogenous TGFβ1,the DC modified with Smad7 pulsed with soluble antigen associated with Lewis pulmonary carcinoma could still induce potent CTL activity against Lewis pulmonary carcinoma,which showed significant difference with DC-Ad-c (P <0.01).Conclusion The inhibitory effects on function of DC of TGFβ may be reversed by blocking the Smad signal of TGFβ pathway.
5.The characteristics of students' evaluation abroad and its enlightenments to China
Jianjun HUANG ; Jidong HUANG ; Jiabang YIN ; Weijun HU
Chinese Journal of Medical Education Research 2005;0(05):-
Students'evaluation has become one of the important means to monitor and guarantee internal teaching quality in universities both at home and abroad.Rich experience has gathered from the theoretical research.Based on the analysis of the major characteristics of students'evaluation in American,Canadian and Japanese universities,the article proposes some suggestions for enhancing the students'evaluation effect in China.
6.Construction of national key disciplines and innovative of post-graduate education
Wenli FENG ; Yibin YIN ; Zhiguang TU ; Yaguang WENG ; Jianjun ZHU
Chinese Journal of Medical Education Research 2006;0(07):-
This paper summarizes that to strengthen the construction of national key disciplines Clinical Laboratory Diagnostic and build professional post-graduate education platform for innovation,the exploration and practice of reform have been done in Chongqing Medical University,in the teaching ranks and exercise disciplinary research direction to enhance the level of scientific research and laboratory construction.
7.Influence of the blocking antibodies on paternal T lymphocyte differentiation antigens (CD3,CD4 and CD8) in women with pregnancy induced hypertension
Meiyang GAO ; Jing FU ; Aihua WU ; Wenchao CHEN ; Jianjun YIN ;
Chinese Journal of Perinatal Medicine 2003;0(06):-
Objective To study the association between paternal CD3, CD4 and CD8 antigenecity to their pregnant spouses and the development of pregnancy induced hypertension (PIH). Methods Maternal serum from 15 women with PIH in the third trimester and 82 in normal pregnancies (16 in the first, 32 in the second and 34 in the third trimester) were incubated with paternal T lymphocytes. Monoclonal CD3, CD4 and CD8 fluorescent conjugated antibodies were then added and the percentage of paternal T cell differentiation antigen CD3, CD4 and CD8 were measured by flow cytometry. Results During normal pregnancy, the levels of maternal serum blocking antibodies on paternal CD3, CD4 and CD8 were (4.14?1.02, 2.02 ?0.24, 2.37?1.05)% in first trimester, (-0.29?0.13, 1.03?0.27, 0.65?0.23)% in the second trimester and (-1.33?1.47,0.15?0.01, -1.04? 0.37)% in the third trimester. There were significant difference between them( P
8.The exploration and practice of the leading teachers' responsibility system in laboratory medicine course teaching
Wenli FENG ; Yibing YIN ; Yaguang WENG ; Zhiguang TU ; Jianjun ZHU
Chinese Journal of Medical Education Research 2011;10(8):907-909
For the reform and development of clinical laboratory education, based on the previous course reform, Laboratory Medicine College of Chongqing Medical University has put the leading teachers'responsibility system of laboratory medicine course into practice. In the recent 3 years, the course is better organized.The students are more interested in the course and they communicate more with teachers than ever before. The effect of the course is obvious. The leading teachers' responsibility system in laboratory medicine course should be promoted.
9.Effects of ping-gan qianyang therapy on adrenal gland proteome in migraine rat model with hyperactivity of Hver-yang
Jianjun HU ; Zeqi CHEN ; Guangwei ZHONG ; Wei LI ; Yaohui YIN
Journal of Chinese Physician 2008;10(5):590-593
Objective To explore the molecule mechanisms of ping-gan qianyang therapy on migraine rat model with the hyperactivity of liver-yang.Methods An animal model of migraine with hyperactivity of liver-yang was established through electrical trigeminal ganglion stimulation and administering orally with Fuzi decoction.The total proteins of adrenal#and in the rats were separated by immobilized PH gradient-based two-dimensional gel electrophoresis, and the 2-DE images Was analyzed by PDQuest 7.0 software.Matrix-Assisted Laser Desorption/Ionization-Time Of Flight Mass Spectrometry(MALDI-TOF-MS)and SWISS-PORT and MSDB database were used to identify differential proteins.Result The well-resolution and reproducible 2-DE patterns of rat adrenal glands in normal group,model group and therapy group were obtained.A total of 8 differentially expressed proteins were identified.Conclusion Ping-gan qianyang therapy may effect on migraine by regulating the expression of proteins related to energy metabolism and ubiquitin.
10.Diagnosis and treatment of gastrointestinal stromal tumors: an analysis of 26 cases
Xiaoqiang TAN ; Huaguang WANG ; Jianjun ZHANG ; Wenzhen YIN
Chinese Journal of Primary Medicine and Pharmacy 2008;15(12):1999-2000
Objective To explore the diagnosis and treatment of gastrointestinal stromal tumors(GIST).Methods 26 patients with gastrointestinal stromal tumors in Ji'an Central People's Hospital from 1998 to 2006 Were retrospectively analyzed.Results All patients received operation.Of them,low malignant GIST wag in 17 eases,high malignant GIST in 9.All patients were follwod-up from 6 to 84 months.4 ofthem had local recurrence and metastasis and 5 eases died.Condusion The diagnosis of GIST Was dimcult because of the lack of clinical features.Final diagnosis should be based on IHC pathology.The main choice of treatment Was operation.Glivee could prolong the life and improve the prognosis of the patients in recurrent and metastatic cases.