The pan-English lecture is one of the important measures to run a higher institution,improve the teaching quality and heighten recognition.Manwhile,it also brings up all-wave impact to the medical eduaction and management in China,such as the course system,the teacher education,the combination of practice and theory and so on.And in this article,the author analyzed the influence factors of the medical teaching quality of the overseas students from the points of teachers,content of courses and students.

A stereologic study of lamellar bodies within pulmonary alveolar type II cells in dogs with experimental acute hemorrhagic pancreatitis(group P)and in dogs inje- cted muscularly with choloroquine before inducing pancreatitis (group PI)was carried out.Our findings showed that volumerical density (Vv), surface density (Sv)and numerical density(Nv)of lamellar bodies were decreased by 29.06%,30.28% and 40.75% in the group P as compared with those in the control group (group N), respectively, and their Sv and Nv were decreased by 23.8% and 30.26% in comparison with those in the group PI.The results suggest that the lung injury resulting from acute hemorrhagic pancreatitis was characterized by alveolar surfactant System damage.Dramatical decrease in the number of the lamellar bodies affected the surface tension of alveoli in a certain degree, leading to atelectasis.There was a relationship between lung injury and the effect of PLA2(phospholipidase A2).Choloroquine might play a role in maintaining the number of lamellar bodies.

To evaluate the fatigue behavior of nitinol stents, we used the finite element method to simulate the manufacture processes of nitinol stents, including expanding, annealing, crimping, and releasing procedure in applications of the clinical treatments. Meanwhile, we also studied the effect of the crown area dimension of stent on strain distribution. We then applied a fatigue diagram to investigate the fatigue characteristics of nitinol stents. The results showed that the maximum strain of all three stent structures, which had different crown area dimensions under vessel loads, located at the transition area between the crown and the strut, but comparable deformation appeared at the inner side of the crown area center. The cause, of these results was that the difference of the area moment of inertia determined by the crown dimension induced the difference of strain distribution in stent structure. Moreover, it can be drawn from the fatigue diagrams that the fatigue performance got the best result when the crown area dimension equaled to the intermediate value. The above results proved that the fatigue property of nitinol stent had a close relationship with the dimension of stent crown area, but there was no positive correlation.
Alloys ; Computer Simulation ; Equipment Failure Analysis ; Finite Element Analysis ; Stents

BACKGROUND:At present, heterologous serum as a medium is a common method for culture of bone marrow mesenchymal stem cells, but it is limited by the disease transmission between species, the potential immune rejection and even controversial ethic issues. This method is also contrary to the requirements of the Ministry of Health. Autologous platelet-rich plasma is a kind of whole blood extract, containing a variety of growth factors. OBJECTIVE:To explore the osteogenic differentiation of rabbit bone marrow mesenchymal stem cells cultured in autologous platelet rich plasma alternative to traditional heterogeneous serum-free culture. METHODS:8 mL bone marrow from the rabbit iliac crest was extracted and anti-coagulated with heparin, and then bone marrow mesenchymal stem cells were isolated using density gradient centrifugation. The cells were divided into autologous platelet rich plasma group (10%autologous platelet rich plasma) and fetal bovine serum group (10%fetal bovine serum). At the passage 4, the cells in the two groups were respectively subdivided into experimental and control groups. Experimental groups were subjected to osteogenic induction, while no change was done in the control groups. cellproliferation was determined by using growth curves;the activity of alkaline phosphatase was detected to adjust the osteogenic differentiation of cells in different groups. RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cells at different generations al showed good proliferation. At 12 days of autologous platelet rich plasma culture, the activity of alkaline phosphatase in the passage 4 cells in the experimental group was significantly higher than that in the control group;while the activity of alkaline phosphatase in the autologous platelet rich plasma group was significantly higher than that in the fetal bovine serum group (P<0.01). These findings indicate that autologous platelet rich plasma as a substitute of xenogeneic serum for culture of bone marrow mesenchymal stem cells is a method characterized as safe and reliable, simple operation, high-purity active induction.

Objectives: To investigate the characteristics and their functional significance of TMJ disc and its attachment. Methods: Each of the 23 TMJs from 12 cadavers were divided into quadrants sagitall y, and total 92 histological slices were obtained and stained with HE.The morpho logical characteristics of the samples was observed microscopically. Results: ①Both temporal posterior and anterior mandibualr attachment s were loose while mandibular posterior and anterior temporal attachments were d ense in which the fibers run anterior posteriorly. ②Fibers in the intermediate zone ran predominantly in anterior posterior direction while those in both ant erior and posterior bands were mixed with transverse fibers. ③At the posterior portion of disc there were 33 out of 92 slices from 6/12 cadavers (11/23 TMJs) s howed the structure of "the fourth band" which was featured by dense fibers alik e those of inner disc. There were 36 out of 92 slices from 6/12 cadavers (10/23 TMJs) showed the structure of "turned fibers" which was featured by a sudden tu rning of fibers from the thick posterior band back and downward, providing an angle like sign. Conclusions: There is great difference o f fiber direction in various parts of disc and its attachments, reflecting the functional demands.

Objective To discuss error factor of the manufacture on baffle plate of radiation field and improve methods of quality assurance and quality control.Methods The errors on different types of baffles were analyzed and evaluated by combining the result of the practice of making baffle and testing of baffle.Results As the difference on the production process and the method of making baffle,there is different degree error on baffle plate of radiation field.The extreme consequence was influenced by quality grate of every manufactured link.Conclusion The technical level of personal,the accuracy of making methods,the equipment precision and the material quality can be produced varying degrees in making.The perfect quality control and quality assurance system can be ensured minimum error.

Objective Human amniotic mesenchymal stem cells (AM-MSCs) transplantation and to investigate the effect of propofol treatment, recovery of brain injury in rats. Methods 80 SD rats, male and female, weight 300-350 g. Human amniotic mesenchymal stem cells cultured in vitro and recovery (CM-Dil) before transplantation standby. The fluid percussion brain injury device, give 2.5-3.0 ATM hydraulic impact, severe fluid percussion brain injury model was made. After injury 6h give corresponding treatment which were randomly divided into 4 groups: injury group (medium injection group), AM-MSCs transplantation group and propofol group, AM-MSCs + propofol group. The number of positive cells in each group was observed under the fluorescence microscope using CM-Dil markers. 4 weeks after transplantation, 6 rats were randomly executed, and RT-PCR and Blot Western were used to detect the changes of GAP-43, AQP4 gene expression and protein synthesis in brain tissues. After transplantation, 24 h, 3 days and 1, 2, 3, 4 weeks for animal neurological deficit score, in 4 weeks after transplantation of Morris water maze (Morris Water Maze, MWM) test. They were killed after four weeks by immunohistochemistry, HE staining. Results Number of CM-DIL positive cell was the most in AM-MSCs + propofol group, which was in propofol group and AM-MSCs transplantation group were lower than that in the AM-MSCs + propofol group, which in injury group was the least, and the differences among the groups had statistically significant (P<0.05). 4 weeks after transplantation, AQP4 and its mRNA expression of brain damage surrounding tissue in injury group were higher than those in AM-MSCs transplantation group and propofol group; which in AM-MSCs transplantation group and propofol group was higher than those in AM-MSCs + propofol group (P< 0.05); GAP-43 protein and mRNA expression in injury group were lower than those in AM-MSCs transplantation group and propofol group, which in AM-MSCs transplantation group and propofol group were lower than those in AM-MSCs + propofol group (P<0.05). 1 weeks after transplantation, rat nerve dysfunction score in AM-MSCs + propofol group was lower than that in propofol group and AM-MSCs transplantation group; which in propofol group and AM-MSCs transplantation group was lower than that in the control group (P<0.05). The times of crossing platform in AM-MSCs + propofol group was higher than that in propofol group and AM-MSCs transplantation group, significantly higher than that in the injury group (P<0.01). The pathological section was observed 4 weeks after injury, there was no axonal passage in the injury group. In propofol group and AM-MSCs transplantation group, a few axonal like structures were observed, more axonal like structures were observed in AM-MSCs + propofol group. Conclusion Propofol combined with human amniotic mesenchymal stem cells transplantation can significantly improve the neurological function of rats with brain injury.

Objective To compare the difference of the spinal cord injury rehabilitation physician training systems between Harvard Uni-versity and Capital Medical University. Methods The spinal cord injury rehabilitation physician training systems of Capital Medical Univer-sity and Harvard University were investigated, including the degree training time, residence training time, training fee and institution. Re-sults Degree education time was more in the basic science in Harvard University than in Capital Medical University. The residence training time was 7 years and 5 years for Harvard University and Capital Medical University, respectively, and the ratio of specialties and sub-spe-cialties was not significantly different. There was a total of 26 spinal cord injury community rehabilitation clinics in Harvard University sys-tem, and none in Capital Medical University. Conclusion Some light can be drawn from the spinal cord injury rehabilitation physician train-ing system of Harvard University, to improve the scholastic building of Capital Medical University.

As introduced in the paper, the average days of stay of clinical department were calculated as a management target, in view of the complexity of disease and case mix index. This method could avoid the deficiency of traditional methods and make the management of average length of stay of clinical department more conforming to the actual situation, and also more scientific and reasonable.

BACKGROUND:Human leukocyte antigen F-associated transcription factor 10 (FAT10) is highly expressed in many tumor cel s like colon cancer cel s, but its relationship with esophageal cancer is less reported. OBJECTIVE:To investigate the effects of siRNA interference technique on the invasion, apoptosis and the characteristics of EC9706 cel s, a human esophageal cancer cel line. METHODS:siRNA sequence was designed and synthesized according to the FAT10 mRNA encoding sequence, and the EC9706 cel s were transiently transfected. EC9706 cel s were divided into three groups:siRNA FAT10 group, negative control group, and blank control group. The expression levels of bcl-2 and FAT10 were detected by RT-PCR and western blot assay, respectively. Cel counting kit-8 assay was used to measure the proliferation of cel s in vitro. Flow cytometry was used to observe the changes of cel cycle, cel apoptosis and the expression of CD44+CD133+. TUNEL staining was used to detect the apoptosis of the cel s. Cel invasion in vitro was detected by Transwel invasion assay. RESULTS AND CONCLUSION:RT-PCR and western blot findings showed that compared with the negative control group and blank control group, the expression levels of bcl-2 and FAT10 mRNA and protein were significantly decreased in the siRNA FAT10 group (P<0.05);the percentage of CD44+CD133+cel s was decreased significantly (P<0.05);and significantly increased apoptosis rate, and decreased cel proliferation and invasion were also found in the siRNA FAT10 group (P<0.05). In conclusion, the specific silencing of FAT10 gene can reduce the invasion of esophageal cancer cel s, inhibit cel proliferation, reduce bcl-2 expression, and increase the apoptosis rate. Meanwhile, the proportion of CD44+CD133+cel s is decreased.