Objective:The objective of this study is to detect the effects of normothermic,mild hypothermic,hypothermic cardiopulmonary bypass(CPB) on somatosensory,cortical evoked potential(SCEP) and super microstructure of cortex in rabbits. Methods:Thirty new zealand white rabbits,randomly allocated into three groups;group A:normothermia CPB(36℃～37℃); group B:mild hypothermia CPB (32℃～34℃);group C:hypothermia CPB (26℃～28℃);Made the open-heart CPB model, maintaining HCT 20%～25%,the duration of CPB is 2 hours;Stimulating from ischiadic nerve,recording SSEP by the time of the end of CPB,The animals were then killed and the cortex were removed for electro microscopic examination.Results:The latency of group C is longer than group B,the latency of group B is longer than the group A,compare between each group (P

Objective To research the clinical effect and operation way of total knee arthroplasty for severe knee valgus deformity. Methods From March 2011 to September 2013, 17 cases (23 knees) with severe knee valgus deformity were selected as the research ob-ject. Metered the tibial angle and checked the activity of knee joint of all the patients before operation. All patients were scored with HSS knee scoring system. Through the selection of lateral parapatellar approach, targeted osteotomy, selective lateral soft tissuelax, patients were given total knee arthroplasty for the treatment of severe knee valgus deformity. The femoral and tibial angle of patients was measured again after the operation. All patients were reexamined after 6 months which included checking the activity of knee joint, evaluating the therapeutic effect of patients with HSS knee scoring system. Results All patients were reexamined in our hospital 6 months after surgery, the degree of femoral and tibial angle decreased from (28. 4 ± 4. 9)° to (5. 2 ± 2. 3)°; knee joint range of motion increased from (65. 4 ± 16. 7)° to (106. 7 ± 27. 3)°;HSS score increased from (38. 46 ± 3. 75) point to (87. 41 ± 4. 77) point. Postoperative indexes were significantly im-proved compared with preoperative indexes, and the differences were of statistical significance (P<0. 05). Conclusion The curative effect is remarkable for patients with severe knee valgus deformity using patellar medial approach, standard osteotomy combined with selective soft tissue release, and total knee arthroplasty. And the knee valgus deformity of patients was corrected and the quality of life has been signifi-cantly improved.

Objective: To observe the efficacy of mirtazapine in the treatment of depression after cerebral infarction and its effect on rehabilitation of neurological functions. Methods:117 patients with acute cerebral infarction comorbid with major depression were randomly allocated to two groups treated with mirtazapine (57 cases) or not (60 cases). Hamiltion Depression Rating Scale (HAMD), Zung's Self-rating Depression Scale(SDS), modified Edingburgh-Scandinavian Stroke Scale (SSS) and Activity of Daily Living(ADL) were measured at baseline, 2 weeks, 4 weeks, 8 weeks and 6 months after randomization.Results:At the end of 6 months trial, the effective rate for depression of mirtazapine group was 100%, including 41 with relief (41/57, 71.9%); while that of control group was 13.4% (3/60), with only 4 with relief (6.7%). For neurological function, 78.9% (45) patients in mirtazapine group had significant improvement, that number in control group was 31 (51.7%). From the third week, patients in mirtazapine group had better ADL results than baseline (31.2?11.2/39.2?15.8), at the end of 6 months, their activity of daily life was much better than that of control (15.7?5.4/21.8?9.7, t=4.17,P

Objective To compare adaptive support ventilation (ASV) and pressure-controlled assist/control ventilation (PCMV) and evaluate the clinical effects of ASV. Methods 12 cases of acute respiratory failure patients induced by all causes were selected in the study. All patients with breathing independently underwent endotracheal intubation. The patterns of mechanical ventilation was PCMV-ASV-PCMV. the two settings of PCMV were uniformity. With ASV mode mechanical ventilation,we adjusted the percentage of minute ventilation (MV%) and ideal body weight (IBW) of patients,so as to reach the same minute ventilation volume of PCMV. We compared the blood gas,respiratory mechanics,work of breathing(WOB),airway occlusion pressure (P0.05). Compared with the first of PCMV,various indicators and blood gas of ASV had no significant difference,work of breathing (WOB),airway occlusion pressure (P=0.1) and inspiratory pressure time product (PTP) were significantly lower (P0.05). Conclusion ASV can get similar goals with conventional ventilation,and reduce patient′s effort and the central respiratory drive.

Objective To investigate the changes of visual evoked potentials (VEP) in patients with Parkinson’s disease (PD). Methods Pattern reversal checkerboard VEP and the unified Parkinson’s disease rating scale (UPDRS) were evaluated in 69 patients with PD and 61 healthy controls. The 69 patients and 61 healthy controls were divided into an aged group (≥60 years old) and a non aged group (0.05). Conclusions VEP reflected objectively the patients’ electrophysical changes of the visual pathway due to dopamine changes and it may be helpful in PD diagnosis.

BACKGROUND:Neural stem cells(NSCs)have the potential of self-proliferation and multiple directional differentiation,and can differentiate into various cells in the neural system under a certain condition.Therefore,NSCs have good prospect in repair of nerve injury.However,RNA interference avoids the abuse of permanent gene silencing,and is hopeful to combine with NSC transplantation for treating craniocerebral injury.OBJECTIVE:To determine whether the Nogo-66 receptor(NgR)gene silencing in NSCs can enhance curative effects of stereotaxic intracerebral transplantation of NSCs on traumatic brain injury(TBI)in rats.METHODS:A total of 60 male Wistar rats following TBI establishment were randomly assigned to 3 groups(n = 20).In the experimental group,NgR gene silencing NSC suspension(6 μL)was injected into rat brain tissue following 24 hours of model induction.In the control group,an equal volume of NSC suspension was infused by the same method.In the blank group,an equal volume of medium without stem cells was infused by the same method.At 24 hours,3 days,1 and 2 weeks following injury,neurological deficits were scored.Two weeks later,animals were sacrificed and subjected to immunohistochemistry and hematoxylin-eosin staining.RESULTS AND CONCLUSION:Following transfection of small interfering RNA,compared with control group,NgR gene protein expression was significantly reduced in the experimental group.At 1 and 2 weeks following transplantation,neurological deficit score was significantly leas in animals undergoing NSC transplantation in the experimental group compared with the control group(P＜0.05).Moreover,neuron number in the brain tissue sections of experimental group was significantly more than in the control group(P＜0.01).At 2 weeks following injury,hematoxylin-eosin staining showed that brain tissue breakage at damaged site as scar connection,remarkable porosis in the blank group;typical morphological changes as neural cells at the transplanted site in the control group;typical morphological changes as neural cells without cavity in the experimental group.Immunohistochemistry showed(37.92±16.02)BrdU-labeled positive cells/high-power field in the blank group,(89.68±15.34)cells/high-power field in the control group,and(102.67±13.52)cells/high-power field in the experimental group.Significant differences were detected between groups(P＜0.01).The above-mentioned results indicated that the NSCs of NgR gene silencing transplanted into the injured cerebral tissues can significantly improve the neurological function in rats with TBI.

Objective To explore the effect of lactational TCDD exposure alone on growing development and cytochrome P4501A1(CYP1A1) in mice offspring. Methods Mature Kunming mice (clean animal,No.604017) were divided to 2 treatment groups (40 and 20 ?g /kg bw TCDD),2 vehicle controls and 1 normal control. There were 3 maternal mice and 25-28 pups in each group. Maternal mice were administered TCDD by intraperitoneal injection in three times on post-parturition days 1,3,5,and the mice offspring were exposed to TCDD by maternal milk. The changes of body weight and the development of reproductive system were observed. The pups were sacrificed on postnatal days (PND) 35,and CYP1A1 expression in the lungs of mice offspring were measured by immunohistochemistry. Results The average body weight of mice offspring in 2 TCDD treatments decreased significantly from PND14 (P

A special method of clinical tumor radiation therapy,namely 3DCRT& IMRT,is described in this paper,including its design and clinical application.

BACKGROUND:Research has been proved that Fasudil,a Rho kinase inhibitor,can effectively inhibit the onset of secondary spinal cord injury.OBJECTIVE:To investigate the synergistic effect of bone marrow masenchymal stem cell (BMSC) transplantion and Fasudil on motor functional recovery following spinal cord injury.DESIGN,TIME AND SETTING:A randomized controlled animal experimant was performed at institute of Endocrinology,Tianjin Medical University from November 2008 to March 2009.MATERIALS:A 1-month-old SD rat was obtained to extract BMSCs.Another 30 healthy female SD rats were used to establish spinal cord injury models,and they were then randomly divided into single injury group,cell transplantation group,and cell transplantation + Fasudil group,with 10 rats for each group.Fasudil was provided by Tianjin Hongri Pharmaceutical Co.,Ltd.METHODS:One week after modeling,spinal cord injury was exposed in the cell transplantation group and cell transplantation+Fasudil group,and 10 μL BMSC suspension was inserted into the injured region.Otherwise,6 hours later rats in the cell transplantation +Fasudil group were treated with an intraperitoneal injection of 10 mg/kg Fasudil,twice a day for one successive week.MAIN OUTCOME MEASURES:Hindlimb motor function was detected using inclined plane test.The Phosphorylated-ERM protein expression was detected by hernatoxylin-eosin staining,pathology and horseradish peroxidase (HRP) nerve trace,and Western blot.RESULTS:Eight weeks after modeling,degree of inclined plane was significantly increased in cell transplantation group and cell transplantation + Fasudil group compared with single injury group (P ＜ 0.05,P ＜ 0.01);while the increased value in the cell transplantation group was significantly greater than cell transplantation + Fasudil group (P ＜ 0.05).Broken myeloid tissue and cavitation were observed in the single injury group;a few of neuraxis-like structures were observed in the cell transplantation group and cell transplantation + Fasudil group,but the cavity in the cell transplantation group was larger than cell transplantation + Fasudil group.HRP-pesitive nerve fibers were detected at T_8 segment or even above in the single injury group and increased in cell transplantation group,in particular in cell transplantation + Fasudil group.Phospho-ERM protein expression in the single injury group and cell transplantation group was significantly greater than cell transplantation + Fasudil group (P ＜ 0.05).CONCLUSION:BMSC transplantation can promote hindlimb motor functional recovery following spinal cord injury,while the combined application of cell transplantation and Fasudil may cause a synergistic effect.

Objective:To construct the sho1 gene mutant of Aspergillus fumigatus,and to investigate the function of sho1 in adaptation to environmental changes.Methods:Saccharomyces cerivisiae sho1 gene homolog was identified by tblastn searches in A.fumigatus genome database.A PCR fragment,composed of the 5' flanking sequence(approximately 1 kb) of sho1,sho1 ORF,and its 3' flanking sequence(approximately 1 kb),was subcloned into a vector pDHt/SK2 with ApaⅠ/Xba Ⅰ enzyme to produce a recombinant plasmid PA.The selected marker pyrG cut from pLAX223 with Nco Ⅰ/Pst Ⅰ was cloned into PA,and PB was produced.The plasmid PB was transformed into competent Agrobacterium tumefaciens EHA105 by using the freeze/thaw method.The strain of A.tumefaciens was designated as At sho1.Then sho1 was produced via ATMT.The radial growth was observed and compared between sho1 and wild type(wt) on MM,CM and BHI medium,and the sensitivity of sho1 and wt to amphotericin B,itraconazole,voriconazole,and caspofungin investigated.Results:Radial growth had visible difference between sho1 and wt on MM,CM and BHI medium.sho1 mutant was sensitive to high osmotic pressure but not to low osmotic pressure.The susceptibility to antifungal drugs was similar between the sho1 and wt.Conclusion:Slow growth of the sho1 mutant has nothing to do with the different medium.The sho1 gene plays a role in response to osmotic pressure in A.fumigatus,but sho1 has a minor role in the susceptibility to the antifungal drugs despite the slow growth.