This paper aims to summarize the clinical application of interventional technique via portal venous system, laying stress on the necessity of reinforcing, improving and comprehensively applying this interventional technique, and to provide new conception concerning the diagnosis and therapy of the diseases involving portal venous system.

Objective To study the effect of psychological nursing on heart rate variability (HRV)in patients with acute myocardium infarction. Methods 80 patients with AMI were randomly divided into group A(routine nursing group)and group B (psychological nursing group) with 40 patients in each group,HRV was evaluated in both groups.The time domain and frequency domain of HRV of 24 h sinus rhythm in two groups of patients were analyzed. Results In group B,24 h average standard deviation of RR interval (SDNN),5 min segments of continuous average standard deviation of RR intervals (SDANN),the difference between adjacent cardiac RR interval of the root mean square (rMSSD),the percentage of adjacent canrdiac RR interval greater than 50 ms (PNN50),low frequency (LF),high frequency (HF) and other HRV parameters were significantly higher than group A. Conclusions Psychological nursing can significantly increase HRV and improve the electric stability of the ischemic myocardium.Psychological nursing can stabilize emotion of patients,decrease sympathetic tone,and may have a role in the prevention of sudden death.

Objective:To study the mechanisms of chronic injury in heart allograft with chronic rejection.Methods:A model of cardiac chronic rejection in the rat based on tolerance induced through donor specific blood transfusions was used.The subpopulation and the distribution of infiltrating cells and the expression of adhesion molecule and growth factors were studied by immunohistochemistry and Northern blot analysis in the chronic rejection model.Results:Infiltrating cells were predominantly T lymphocytes and macrophages,mostly evident in the interstitial,subendocardial and perivascular areas.Allografts were demonstrated significantly elevated number of CD45+,CD4+,CD8+,TCR+,CD45RB+ cells,macrophages(ED_1) and NK cells at both 3 and 6 months compared with normal rat hearts and isografts at 3 months.Damaged areas also were observed expression of the adhesion molecules ICAM-1,LFA-1,basic fibroblast growth factor (bFGF) and transforming growth factor(TGF-?1).Northern blot analysis of total RNA,showed 5 to 6 fold upregulation of TGF-?1 mRNA in the chronic rejection model compared with normal rat hearts.Conclusion:These results suggest that T lymphocytes and macrophages play a central role in the development on chronic rejection and the increased expression of ICAM-1,LFA-1,bFGF and TGF-?1 in this model supports the involvement of these adhesion molecules and growth factors in the development of cardiac allograft vasculopaphy.

Desmoid tumor of abdominal wall was also known as fibronmatosis of abdominal wall.It is a rare benign fibroblastic tumor, which is characterized by non-enveloped,strong local infiltration, with high recurrence rate after surgery.In December 2011, one patient with huge desmoid tumor of abdominal wall with intestinal tube received surgical resection with single-stage patch repair for the large defect in abdominal wall at the 210th Hospital of PLA, and the result was satisfactory.

Objective To analyze the epidemiological trends of leprosy and evaluate the effectiveness of control in Zhejiang Province. Methods A retrospective study was conducted. Results By the end of 1999, 16 262 leprosy patients had been registered, of them 11 935 had been cured and only 97 were still remained. In 1999, active cases decreased by 99.02％ over 1973. The epidemiological trends of leprosy showed the increase of mean age at onset and type ratio, and the decrease of incidence in children. Conclusion The epidemicity of leprosy has been continuously dropping in Zhejiang Province due to the sustained effort and effective control.

OBJCTIVE:To determine the content of Gentiopicrin in Qianggan capsules by HPLC.METHODS:The sample was separated on Diamonsil TM C18 (200 mm?4.6 mm,5 ?m) with mobile phase consisted of methanol-water (23∶77) at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 254 nm.RESULTS: The linear range of Gentiopicrin was 0.018 59～0.232 40 ?g(r=0.999 6) with an average recovery of 96.59%(RSD=1.35%,n=6).CONCLUSION: The meth-od developed is accurate,stable and reproducible,and it provides basis for the improvement of the quality standard of Qianggan capsules.

To study the effectiveness of gene therapy for acoustic trauma induced by intensive noise, the replication defective adenovirus that carries human neurotrophin 3 cDNA(Ad NT3) was inoculated into the guinea pig cochlea 3 days after exposure. ABR and CAP were recorded after 14 days and 2 months, respectively. No difference was found 14 days after exposure. At the end of two months, the Ad NT3 treated animals showed less ABR threshold shift, lower CAP threshold and shorter CAP N1 latency by 100dBSPL click. The result suggested that Ad NT3 could be used to express the neurotrophins within the cochlea and reduce noise induced hearing loss in vivo .

Objective To compare adaptive support ventilation (ASV) and pressure-controlled assist/control ventilation (PCMV) and evaluate the clinical effects of ASV. Methods 12 cases of acute respiratory failure patients induced by all causes were selected in the study. All patients with breathing independently underwent endotracheal intubation. The patterns of mechanical ventilation was PCMV-ASV-PCMV. the two settings of PCMV were uniformity. With ASV mode mechanical ventilation,we adjusted the percentage of minute ventilation (MV%) and ideal body weight (IBW) of patients,so as to reach the same minute ventilation volume of PCMV. We compared the blood gas,respiratory mechanics,work of breathing(WOB),airway occlusion pressure (P0.05). Compared with the first of PCMV,various indicators and blood gas of ASV had no significant difference,work of breathing (WOB),airway occlusion pressure (P=0.1) and inspiratory pressure time product (PTP) were significantly lower (P0.05). Conclusion ASV can get similar goals with conventional ventilation,and reduce patient′s effort and the central respiratory drive.

Objective The selection of SPE column to extract trace pesticides in the water. Methods Investigated the recovery of 6 kinds SPE column (including HLB,GDX403,GDX103,C18,C8,C6) to extract phorate, sulfotep, propoxur, carbofuran, cypermethrin, fenvalerate in the water. Results By using oasis HLB cartridge to extract 21 kinds of pesticides in water, the recoveries were over 60%for the most pesticides. Conclusion The method could be applied to the cases which extract and analyse the trace pesticides in large volume water.

Objective To investigate the characteristics of serum lipids levels in young women with paraplegia in sedantary lifestyles.Methods 183 female paraplegics who admitted in China Rehabilitation Research Centre (CRRC) from Jan 2004 to Dec 2009 were investigated.Lipid profiles included triglyceride (TG), total cholesterol (TC), low density lipoprotein (LDL), and high-density lipoprotein (HDL).Results Female paraplegics showed higher mean TG and TC levels than mean levels of Chinese females (TG 1.44 mmol/L vs. 1.24 mmol/L; TC 4.45 mmol/L vs. 4.30 mmol/L). But no difference was found in HDL and LDL levels. TG, TC and LDL levels were correlated positively to age (r=0.158, P<0.05; r=0.271, P<0.01; r=0.207, P<0.01, respectively), but HDL showed no correlation to age. Prevalence of dislipidemia was 60%, including low HDL(33%), high TC(25%), high TG(24%), and high LDL(21%). Conclusion Lack of exercise may result in some dyslipidemia and increase cardiovascular risks in women who are under estrogen protection.