Objective Human amniotic mesenchymal stem cells (AM-MSCs) transplantation and to investigate the effect of propofol treatment, recovery of brain injury in rats. Methods 80 SD rats, male and female, weight 300-350 g. Human amniotic mesenchymal stem cells cultured in vitro and recovery (CM-Dil) before transplantation standby. The fluid percussion brain injury device, give 2.5-3.0 ATM hydraulic impact, severe fluid percussion brain injury model was made. After injury 6h give corresponding treatment which were randomly divided into 4 groups: injury group (medium injection group), AM-MSCs transplantation group and propofol group, AM-MSCs + propofol group. The number of positive cells in each group was observed under the fluorescence microscope using CM-Dil markers. 4 weeks after transplantation, 6 rats were randomly executed, and RT-PCR and Blot Western were used to detect the changes of GAP-43, AQP4 gene expression and protein synthesis in brain tissues. After transplantation, 24 h, 3 days and 1, 2, 3, 4 weeks for animal neurological deficit score, in 4 weeks after transplantation of Morris water maze (Morris Water Maze, MWM) test. They were killed after four weeks by immunohistochemistry, HE staining. Results Number of CM-DIL positive cell was the most in AM-MSCs + propofol group, which was in propofol group and AM-MSCs transplantation group were lower than that in the AM-MSCs + propofol group, which in injury group was the least, and the differences among the groups had statistically significant (P<0.05). 4 weeks after transplantation, AQP4 and its mRNA expression of brain damage surrounding tissue in injury group were higher than those in AM-MSCs transplantation group and propofol group; which in AM-MSCs transplantation group and propofol group was higher than those in AM-MSCs + propofol group (P< 0.05); GAP-43 protein and mRNA expression in injury group were lower than those in AM-MSCs transplantation group and propofol group, which in AM-MSCs transplantation group and propofol group were lower than those in AM-MSCs + propofol group (P<0.05). 1 weeks after transplantation, rat nerve dysfunction score in AM-MSCs + propofol group was lower than that in propofol group and AM-MSCs transplantation group; which in propofol group and AM-MSCs transplantation group was lower than that in the control group (P<0.05). The times of crossing platform in AM-MSCs + propofol group was higher than that in propofol group and AM-MSCs transplantation group, significantly higher than that in the injury group (P<0.01). The pathological section was observed 4 weeks after injury, there was no axonal passage in the injury group. In propofol group and AM-MSCs transplantation group, a few axonal like structures were observed, more axonal like structures were observed in AM-MSCs + propofol group. Conclusion Propofol combined with human amniotic mesenchymal stem cells transplantation can significantly improve the neurological function of rats with brain injury.

Vojta治疗法, 脑性瘫痪, 中枢性协调障碍, 综述

BACKGROUND:Neural stem cells(NSCs)have the potential of self-proliferation and multiple directional differentiation,and can differentiate into various cells in the neural system under a certain condition.Therefore,NSCs have good prospect in repair of nerve injury.However,RNA interference avoids the abuse of permanent gene silencing,and is hopeful to combine with NSC transplantation for treating craniocerebral injury.OBJECTIVE:To determine whether the Nogo-66 receptor(NgR)gene silencing in NSCs can enhance curative effects of stereotaxic intracerebral transplantation of NSCs on traumatic brain injury(TBI)in rats.METHODS:A total of 60 male Wistar rats following TBI establishment were randomly assigned to 3 groups(n = 20).In the experimental group,NgR gene silencing NSC suspension(6 μL)was injected into rat brain tissue following 24 hours of model induction.In the control group,an equal volume of NSC suspension was infused by the same method.In the blank group,an equal volume of medium without stem cells was infused by the same method.At 24 hours,3 days,1 and 2 weeks following injury,neurological deficits were scored.Two weeks later,animals were sacrificed and subjected to immunohistochemistry and hematoxylin-eosin staining.RESULTS AND CONCLUSION:Following transfection of small interfering RNA,compared with control group,NgR gene protein expression was significantly reduced in the experimental group.At 1 and 2 weeks following transplantation,neurological deficit score was significantly leas in animals undergoing NSC transplantation in the experimental group compared with the control group(P＜0.05).Moreover,neuron number in the brain tissue sections of experimental group was significantly more than in the control group(P＜0.01).At 2 weeks following injury,hematoxylin-eosin staining showed that brain tissue breakage at damaged site as scar connection,remarkable porosis in the blank group;typical morphological changes as neural cells at the transplanted site in the control group;typical morphological changes as neural cells without cavity in the experimental group.Immunohistochemistry showed(37.92±16.02)BrdU-labeled positive cells/high-power field in the blank group,(89.68±15.34)cells/high-power field in the control group,and(102.67±13.52)cells/high-power field in the experimental group.Significant differences were detected between groups(P＜0.01).The above-mentioned results indicated that the NSCs of NgR gene silencing transplanted into the injured cerebral tissues can significantly improve the neurological function in rats with TBI.

Objectives:To evaluate the clinical significance of different early nutrition support in severe head injured patients. Methods:140 cases(GCS≤8) were randomly divided into 5 groups.From A to E,each one had 28 cases.The patients in group A received early parenteral nutrition(PN) and enteral nutritoin(EN) together;group B received early PN,and EN after 1 week later;group C received PN only for more than 2 weeks;group D received early EN only;group E received traditional delayed EN.The clinical observation and statistical comparison were taken. Results:Severe head injured patients could get nutrition from different ways at early stage.A and B groups had better outcomes,and the blood glucose and nutritional data were superior to those of D,E group( P

Objective To observe the clinical efficacy of acupuncture, chiropractic plus functional exercise of lumbar and gluteal muscles in treating postpartum sacroiliac malposition.Method Forty patients with postpartum sacroiliac malposition were randomized into group A, group B, group C and group D, 10 cases in each group. Group A was intervened by acupuncture, chiropractic plus functional exercise of lumbar and gluteal muscles, group B by acupuncture plus chiropractic, group C by acupuncture plus functional exercise of lumbar and gluteal muscles, and group D by chiropractic plus functional exercise of lumbar and gluteal muscles. The four groups were intervened once every other day. The modified Oswestry Disability Index (ODI) score was observed before and after 5 treatment sessions, and the clinical efficacies in the four groups were compared.Result The total effective rates were 100.0% in the four groups, and the inter-group differences were statistically insignificant (P>0.05). The ODI scores in the four groups were significantly changed after treatment and in the 3-month follow-up study compared with those before the treatment (P<0.05). After the treatment, the ODI score in group B was significantly different from that in the other groups (P<0.05). The ODI scores in group A and B in the 3-month follow-up study were significantly different from those after the treatment in the same group (P<0.05). The ODI score in group A was significantly different from that in the other groups in the 3-month follow-up study (P<0.05). The ODI score in group B in the 3-month follow-up study was significantly different from that in group C and D (P<0.05).Conclusion Acupuncture, chiropractic plus functional exercise of lumbar and gluteal muscles is definitely effective in treating postpartum sacroiliac malposition, acupuncture plus chiropractic can produce a significant short-term efficacy and alleviate the symptoms, and functional exercise of lumbar and gluteal muscle can produce an obvious long-term efficacy.

A retrospective study and cause analysis on the emergency rescue and medical service during the 2015 Tianjin harbor explosion accident,probed into the defects of the contingency plan of the hospital.Problems found include impracticability or deficiency of the plan,lack of safety awareness,and absence of preventive measures.A cause analysis was thus made to propose the following improvements on the contingency plan:1.Development of specific standard operation procedures(SOPs)for medical emergency response,including SOP for emergency response organization and command,that for the procedure and responsibility of massive injuries examination,and that for emergency rescue;2. Development of the plan for data statistics,that for volunteer management,that for media communications and that for donation management.

Objective To observe the effects of subarachnoid block on plasma nitric oxide level of the pregnancy induced hypertension patients Methods Twenty female patients ASA class I ,scheduled for elective gynecological surgery served as non pregnancy group, 20 normal late stage gravidas as pregnancy group,and 20 severe pregnancy induced hypertension(PIH) gravidas as PIH group The intravenohs blood samples were taken immediately before subarachnoid block and 10 min following subarachnoid block(before incision),to quantify the plasma concentration of nitric oxide by measuring the metabolic production of nitric oxide:nitrite and nitrate Results Immediately before subarachnoid block,the plasma nitric oxide level decreased significantly in non pregnancy group and PIH group compared with that in pregnancy group (P

Objective To develop a method detecting hMAM gene expression of breast cancer cell lines in peripheral blood.Methods The hMAM gene was cloned and confirmed by agarose gel electrophoresis and sequence analysise,the standard substance of real-time quantitative polymerase chain reaction (FQ-PCR )was prepared. The breast cancer cell lines MDA-MB453、MCF7 were cultured and the blood sample model was made.The hMAM expression of breast cancer cell lines blood model and 10 cases of breast cancer patient peripheral blood were detected by RT-PCR and FQ-PCR,GAPDH gene expression as contrast.Results The production of PCR was cloned and testified by sequence.The correlation coefficient(r) of standard curve line of FQ-PCR was -1.0. The x?s and CV were 718.9?120.5 and 16.7% respectively.The hMAM expression of one breast cancer cell lines MDA-MB453 in 104 blood cells could be detected, but not detected in the breast cancer cell lines MCF7. The hMAM expression was detected in 3 of 5 patients with sentinel nodes and in 1 of 5 patients with non-sentinel nodes.Conclusions It might be a good method for detecting breast cancer cell micrometastased in peripheral blood. The diffrernce of hMAM gene expression correlates with the type of breast cancer cell lines.

Objective To evaluate the osteoinductive activity of recombinant adenoviral vector carrying the human BMP-2 gene(AdBMP-2) and investigate the mechanism of bone formation induced by the bone morphogenetic protein 2(BMP-2).Methods AdBMP-2 infected rabbit bone messenchymal stem cells(BMSC) were cultivated in vitro.The expression of hBMP-2 was determined by in situ hybridization and immunohistochemical analysis,and ALP was determined by enzyme cytochemistry 7 d after infection.At 21th day,Alizarin red S staining was used to evaluate calcium-rich deposits by cells transfected in culture.30 ?L AdBMP-2 solution(2?10~(12)pfu?mL~(-1)) was injected into the muscle of the left hind limb of the nude rat,and 30 ?L Ad? gal solution(2?10~(12)pfu?mL~(-1)) was injected into the muscle of the right hind limb as control.Animals were sacrificed at 20th day following injection to investigate the bone formation histologically and the expression of BMP-2 in the specimens of the injection sites by immunohistochemistry and in situ hybridization method.Results The expressions of BMP-2 and ALP in the transduced BMSC were confirmed at 7 th day after infection.A deeply stained mineralized layer was evident at 21th day in transduced cells.In vivo experiment,BMP-2 gene therapy induced mesenchymal monocytes chemotaxis,proliferation, differentiation into chondrocytes,with subsequent endochondral bone formation.At the same time,hBMP-2 expression was observed in the chondrocytes,osteoblasts and mesenchymal monocytes.(Conclusion AdBMP-2) posseses the osteoinductive activity in vitro and in vivo.

Sleep disorders are common diseases with various dys-function during sleep-wake process, including difficulty falling or staying asleep, falling asleep at inappropriate times, excessive total sleep time, or abnormal behaviors associated with sleep. Sleep disorders can lead to the deposition of amyloid beta protein ( Aβ) by affecting the normal metabolism of amyloid beta protein in the brain. Patients with Alzheimer’s disease ( AD) often suffer from sleep disorders, and its pathology always results in more se-vere sleep disorders, which leads to a risk of cognitive impair-ment and hypofunction. Sleep disorders could interact closely with AD, forming a positive feedback loop, which causes serious damage to the body health. This review summarized the current research about sleep disorder in the onset of AD and the current status of medication.