1.Influence of CYP3A5 polymorphism on cydosporin A concentrations in the peripheral blood of hemopoietic stem cell transplant recipients
Hanping WANG ; Ping MAO ; Jianjin XIE
Chinese Journal of Organ Transplantation 1996;0(04):-
Objective To explore the possibility of cytochrome P-450 3A5 (CYP3A5) genotype as a major factor to guide individualized employment of cyclosporin A(CsA) through a comparative study of CsA concentrations in the peripheral blood of hemopoietic stem cell transplant recipients with different CYP3A5 genotypes. Methods Olymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to genotype CYP3A5 gene, and CsA concentrations were detected by a commercial fluorescence polarization immunoassay. Result There were significant differences in CsA concentrations, including standardized trough concentrations C0 and two h peak concentrations C2 , in the two CYP3A5 genotypes found in our samples, and both C0 and C2 in wild homozygotes were lower than heterozygotes. Conclusion CYP3A5 polymorphism is highly associated with CsA concentrations in hemopoietic stem cell transplant recipients, and CYP3A5 genotype may be a predictor to the dose requirement before clinically employment of CsA.
2.Digital Management of Medical Equipment
Jianjin HANG ; Xiangyang WU ; Chaoqun ZHANG ; Qinghua WANG
Chinese Medical Equipment Journal 2004;0(09):-
Relative problems in the digital management of medical equipment are explored.Study contents include digital hospital and digital medical equipment,main digital management fields of medical equipment,new progress in technique of digital management.It is pointed out that the digital management of medical equipment is an important part of hospital digital construction,which needs discussion and improvement in practice.
3.Database Storage and Retrieval of Medical Equipment Images in PowerBuilder
Xiangyang WU ; Chaoqun ZHANG ; Jianjin HANG ; Qinghua WANG
Chinese Medical Equipment Journal 2003;0(10):-
Objective To access images of medical equipments in database management system. Methods Images in SQL Server2000 can be stored inside database tables directly as the binary content of a Blob field. Under the PowerBuilder development environment, SelectBlob and UpdateBlob are used to perform the data exchange between the Blob field and a Blob variable defined in Powerscript,then image contained in the Blob variable can be constructed in a picture control available in PowerBuilder. Results It is convenient and expeditious to insert, update and present images in this PowerBuilder application. Conclusion Processing images in the medical equipments database management system extends its functions and has a significant applied value.
4.Implementation of Multi-condition Combination Query Based on PowerBuilder Datawindow
Xiangyang WU ; Chaoqun ZHANG ; Jianjin HANG ; Qinghua WANG
Chinese Medical Equipment Journal 2003;0(12):-
Objective To perform multi -condition combination query in the browse window based on PowerBuilder datawindow technology. Methods After the interested combination of query conditions specified on certain columns in datawindow was constructed by if...then statement, the setfilter () and filter () function was used to retrieve and display data which met the filter criteria. Results A flexible query object was incorporated into the data browsing window. Conclusion The datawindow-based query is suitable for situations where batch of data are filtered against not so many columns.
5.Application and developing trend of biomedical sensor
Jianjin HANG ; Qinghua WANG ; Xiangyang WU ; Fanglin ZHANG
Chinese Medical Equipment Journal 1989;0(01):-
Biomedical sensor is a key component to the modern medical instrument,which can convert physiological value to electrical signal.After introducing and analyzing the definition,main uses and present application of biomedical sensor,this paper lays emphasis on its developing trend.
6.Immunophenotypic analysis and identification of hematogones and acute B lymphoblastic cells by flow cytometry
Yanli XU ; Shunqing WANG ; Qinghua DU ; Jianjin XIE
Journal of Leukemia & Lymphoma 2015;24(5):282-286
Objective To discriminate morphology and immunophenotype differences between hematogones and lymphoblast to provide some references for the correct identification of hematogones and minimal residual leukemia cells.Methods Immunophenotypes were detected by flow cytometry in a total of 132 bone marrow from 58 patients with acute B lymphoblastic leukemia during diagnosis,remission and relapse.Hematogones were identified based on combination of CD34/CD10/CD19/CD45 or CD34/CD10/CD45/CD19/CD20/CD38.Results Among 132 specimens,45 (34 %) were identified hematogones,the detection range was 0-36 %.Three specimens appeared in diagnosis patients,one in relapse,and the remaining 41 cases in remission.The detection rate of hematogones was 62 % (41/66) in the remission cases.More than 5 % leukemia cells of nucleated cells were detected in diagnosis and relapse,and less than 5 % residual leukemia cells was in 24 specimens from remission patients.In 28 specimens,the co-existence of hematogones and leukemia cells was found,including three in diagnosis,one in relapse and the remaining 24 in remission.Hematogones were characterized in term of variable expression of CD45 and very low side scatter.The early hematogones expressed CD34.With maturation increasing,hematogones gradually lacked CD34.CD19 and CD10 were presented in whole hematogones stage.Early hematogones had expression of CD10.Lymphoblasts showed maturation arrest and more homogeneous populations.SSC values of hematogones were higher than that of normal B cell progenitors.Antigen overexpression or underexpression was not found in normal hematopoietic progenitor B cells,and hematopoietic progenitor B cells did not appear cross-lineage markers,CD20+ cells exhibited continuous distribution from negative to weak positive for normal hematogones.Conclusions Hematogones were present in diagnosis,remission and relapse cases with acute B lymphoblastic leukemia,especially abundant in bone marrow after chemotherapy.It should be careful to diagnose and discriminate the malignant cells from benign cells.By comprehending continuous and complete maturation spectrum of antigen expression for normal hematogones,knowing phenotype of leukemia cells drift change patterns and using multiparameter flow cytometry and optimal antibody combination,it is significant in identifying residual lymphoblasts from hematogones and improving the detection accuracy in minimal residual disease.
7.Relationship between platelet activating factor acetylhydrolase genetic polymorphism and platelet activation and prognosis in patients with ischemic stroke
Jianping ZHOU ; Yuxia SHEN ; Kui YIN ; Chen WANG ; Yuchen QIU ; Wanxiang WANG ; Jianjin DENG
Chinese Journal of Postgraduates of Medicine 2009;32(10):27-31
Objective To investigate the corelation between platelet activating factor acetylhydrolase (PAF-AH) genetic polymorphism and ischemic stroke. Methods The plasma PAF-AH genotype was determined in 205 patients with iachemic stroke and 114 normal subjects by the polymerase chain reaction. The levels of plasma platelet activating factor (PAF), platelet α-granule membrane glycoprotein-140(GMP-140), β-thromboglobulin (β-TG) and the levels of platelet factor 4 (PF4) were analyzed. Results The prevalence of the mutation genotype and plasma PAF, GMP-140, β-TG and PF4 in the patients with isehemic stroke [42.44%,(91.08 ± 39.10) ng/L, (36.46 ± 13.10) μg/L, (41.75 ± 11.18) μg/L, (29.05 ± 9.16) g/L, respectively] were significantly higher than those in the controls[21.05%,(64.30 ± 18.81) ng/L, (18.27 ± 7.68) μg/L, (30.94 ± 8.47) μg/L, (18.75 ± 6.06) μg/L](P< 0.01). The levels of plasma PAF, GMP-140 were significantly higher in mutation genotype patients than those in the normal genotype patients (P < 0.01). Conclusions The activation function of platelet in the acute phase of patients with ischemic stroke increases, and it is associated with genetic polymorphism of PAF-AH. The PAF-AH gene mutation may be a novel genetic marker for high risk of ischemic stroke.
8.Effects of salvianolate on cardiomyocytes apoptosis and heart function in a swine model of acute myocardial infarction.
Mingwei WANG ; Dianfu ZHANG ; Jianjin TANG ; Bo CHEN ; Liansheng WANG ; Zhijian YANG ; Jun HUANG
Journal of Integrative Medicine 2009;7(2):140-4
To observe the effects of salvianolate on cardiomyocytes apoptosis and heart function in a swine model of acute myocardial infarction (AMI).
9.Experimental study on rapid detection of lower respiratory tract pathogenic bacteria by denaturing high-performance liquid chromatography
Xiaoyan ZHANG ; Zhaohui LIU ; Zhike HANG ; Hanping WANG ; Huifen YE ; Yinmei YNAG ; Jianjin XIE
Chinese Journal of Microbiology and Immunology 2008;28(10):951-954
Objective To construct a new molecular biological method for the analysis of microbial species in lower respiratory tract infections based on 16S rRNA gene by denaturing high-performance liquid chromatograph(DHPLC).Methods The universal primer set was analyzed basing on the highly conserved regions of 16S rRNA gene.DNA amplicons of lower respiratory tract were analyzed by DHPLC to generate peak profiles respectively.The incorporation of 40-bpGC clamp into the amplification primet was essential to effectively discriminate genetic differences identification.Results The primers could only amplify bacterial 16S rRNA.Bacterial of amplicons which incorporation of a 40-bpGC clamp were effectively discriminated genetic differences in DHPLC.The results of clinical isolares identification showed 100%according with the traditional method.Conclusion DHPLC has not only high accuracy,but also is a convenient,rapid and high-through technique for the discrimination bacteria.It has potential value in the detection of lower respiratory pathogenic bacteria.
10.Evaluation of left ventricular twist and untwist in patients with apical hypertrophic cardiomyopathy using velocity vector imaging
Xuehong FENG ; Chunyuan YOU ; Chengjian YANG ; Xinhe YE ; Jianjin WANG ; Daqing YANG ; Mengzhu FU
Chinese Journal of Ultrasonography 2011;20(2):112-115
Objective To evaluate left ventricular(LV) twist and untwist using velocity vector imaging(VVI) in patients with apical hypertrophic cardiomyopathy (ApHCM). Methods Twenty-three patients diagnosed with ApHCM were consecutively enrolled and compared with normal controls. After a standard echocardiographic examination, parasternal basal and apical short-axis planes were scanned to quantify LV rotations,twist and LV untwist using VVI. Results Compared with the normal controls, the rotation and rotaional velocity of apical subendocardial myocardium were markedly decreased in ApHCM patients during the period of systole ( P<0.05) ,but the decreases in basal planes were not significant. As a consequence,LV twist was significantly lower in ApHCM patients (P< 0.05). Compared to normal controls,the velocity of LV untwisting was also significantly decreased in ApHCM patients ( P<0.05).Conclusions The twist and untwist of LV subendocardial myocardium were decreased in ApHCM patients.VVI is a useful method to assess the characteristics of LV twist and untwist in ApHCM patients.