Objective To explore the clinical significance of K-ras mutation in non-small cell lung cancer. Methods A K-ras codon 12 point mutation was detected by restriction fragment length polymorphisms combined with polymerase chain reaction in 41 tissues from non-small cell lung cancer (NSCLC), 21 normal tissues from nearby tumor and 21 lymph nodes from nearby bronchi. Results K-ras mutation was detected in 10 of 41 NSCLC((24.39%)). There was no relationship between K-ras mutation and the age, sex, differentiation of tumor, tumor volume,histologic type and tumor stage. K-ras mutation was more common in smoking group than in non-smoking group, but the statistical comparison was not significant. There was a strong link between cigarette smoking and K-ras point mutation in lung adenocarcinoma, but there was no relation between cigarette smoking amount and K-ras mutation. K-ras mutation was found in 4 of 21 metastatic lymph notes. The mortality in positive K-ras mutation was significantly higher than that in negative K-ras mutation(P

Diagnostic Errors ; Humans ; Pneumoconiosis ; diagnosis ; Scleroderma, Systemic ; diagnosis

Objective:In order to find a possible relationship between the polymorphism of HLA A,DRB1 allele and susceptibility to cirrhosis due to hepatitis B.Methods:HLA A,DRB1 allele polymorphism in 61 patients with cirrhosis due to hepatitis B were analyzed by DNA chip,146 healthy subjects as control group were also tested.Results:The most frequent alleles were HLA A02,11,24 for HLA A locus and HLA DRB112,09,04 for HLA DRB1 locus in control group respectively,the significantly increased frequencies of HLA A02(43 4% vs 29 1%,OR:1 87,P=0 005,95% CI:1 21 2 89)与HLA DRB107(13 9% vs 5 1%,OR:3 00,P=0 002,95% CI:1 48 6 07)、HLA DRB108(16 4% vs 6 8%,OR:2 67,P=0 003,95% CI:1 40 5 08)、HLA DRB111(12 3% vs 5 8%,OR:2 27,P=0 025,95% CI:1 11 4 64) were found in cirrhosis patients due to hepatitis B compared to the control group,the homozygotes of HLA A02 showed a tendency to increase in cirrhosis patients,but failed to reach statistical significance.Conclusion:HLA A02 and HLA DRB107、08、11 may be susceptibility genes for cirrhosis due to hepatitis B. [

Objective:To study the prevalence of depressive symptom and related factors among urban primary school students in Hefei.Methods:2,078 primary school students were surveyed with questionnaires including Children's Depression Inventory and items of social support and life style.Results:The prevalence of depressive symptom was 11.9%,the rate of boys was significant higher than that of girls(13.6%/9.6%,?2=7.01,P

Objective: To determine the relationship between resilience and social support among primary school students.Methods: 2 067 primary school students from grade 3 to 5 were surveyed with questionnaires of Social Support Questionnaire and the Student Resilience Survey revised from the California Healthy Kids Survey in Hefei. Results: (1) The average scores of resilience of students in Hefei were 49.0?8.8, Girls got higher scores (50.1?8.6) than boys on resilience(47.9?8.8,t=5.71, P

Objective To study the clinical effect of noninva si ve positive pressure ventilation(NIPPV)on the treatment of acute exacerbations o f chronic obstructive pulmonary disease(COPD)with type Ⅱ respiratory failure.Methods Observe the data in arterial blood gas (pH,PaO2,PaCO2 ),the course of disease in the hospital ,the rate of endotracheal intubation ,the mortality of the patients in NIPPV and compare with those of the patients without NIPPV treatment before.Results The data in arterial blood gas had been improved signif icantly in NIPPV after 24h.The course of disease in hospital was significantly s horter,and the rate of endotracheal intubation and mortality of the patients wa s significantly lower in NIPPV than those in no NIPPV.There was remarkably diffe rence between two groups.In NIPPV,the rate of endotracheal intubation were sig nificantly lower in light and moderate respiratory failure than those in severe respiratory failure(P

The present study was to estimate pharmacokinetic parameters of metformin hydrochloride in 20 Chinese healthy volunteers with a limited sampling strategy (LSS), which will provide scientific data for bioequivalence and clinical application. A single dose of metformin was administrated to 20 healthy volunteers. The concentration of metformin in whole blood was determined by validated high performance liquid chromatography (HPLC) method. Multi-linear regression analysis was performed to establish a model to estimate AUC(0-24 h) and Cmax of metformin by LSS method. The LSS models were validated by the Jackknife method. The result indicated: the linearity relationship between AUC(0-24 h) or Cmax and single concentration point was poor. Several models for metformin AUC(0-24 h) or Cmax, estimation were better (r2 > 0.9, P < 0.05). Validation tests indicated that most informative sampling points (C2, C6 for AUC(0-24 h), C1.5, C2 for Cmax) provided accurate estimations of these parameters. So, a multi-linear regression model for estimation pharmacokinetic parameters of metformin by using LSS method is feasible.

Objective To explore role of functional neck dissection for lower collar-shaped incision at hair margin behind ear in the treatment of differentiated thyroid cancer. Methods The clinical data of 11 patients with thyroid papillary carcinoma who underwent func-tional neck dissection for lower collar-shaped incision at hair margin behind ear were retrospectively analyzed. Results The incisions of all patients were unseen in daily life and patients were satisfied with the postoperative appearances. No case of shoulder syndrome,postoperative local recurrence and distant metastasis was observed. Conclusion Functional neck dissection for lower collar-shaped incision at hair margin behind ear is effective in the treatment of differentiated thyroid cancer. Meanwhile it can obviously improve patients’ postoperative appearance and life quality,therefore it is worth clinical application.

Objective:To explore therapeutic effect of rosuvastatin combined clopidogrel bisulfate tablet on patients with coronary heart disease (CHD).Methods: A total of 124 patients, who were diagnosed as CHD by coronary angiography, were selected, randomly and equally divided into routine treatment group (received routine treatment,including clopidogrel bisulfate tablet) and combined treatment group (received rosuvastatin based on routine treatment group), both groups were treated for six months.Cardiac function, serum levels of angiotensin II (Ang II), vascular endothelial growth factor (VEGF) and nitric oxide (NO), and therapeutic effect were measured and compared between two groups before and after treatment.Results: Total effective rate of combined treatment group was significantly higher than that of routine treatment group (96.77% vs.83.87%, P=0.015).Compared with routine treatment group after treatment, there were significant rise in left ventricular ejection fraction [(61.89±7.02)% vs.(68.96±8.23)%] and NO level [(75.25±9.45) μmol/L vs.(82.25±10.22) μmol/L], and significant reductions in wall motion score index [(1.35±0.39)% vs.(1.11±0.29)%], levels of Ang Ⅱ [(102.25±6.93) ng/L vs.(52.99±5.36) ng/L] and VEGF [(328.25±23.41) ng/L vs.(228.69±22.69) ng/L] in combined treatment group, P<0.01 all.Conclusion: Rosuvastatin combined clopidogrel bisulfate tablet can effectively improve heart function, serum levels of Ang II, VEGF and NO in patients with coronary heart disease, the therapeutic effect is significant, which is worth extending.