Anxiety and depression has become the most popular state of mind and body in people in transition.Chinese in Heaven,body and apirit unified,coordinated organs,under the guidance of the theory of yin and yang balance,through diagnosis and treatment,the diseases have psychosomatic coherent advantage.This article discussed the TCM mechanism of anxiety and depression disorders from the aspects of Chinese five internal organs and emotions,the five organs system function and emotion caused disease,and finally proposed the treatment principle of reconciliation with cardinal Yin and Yang which may offer clinical references to anxiety and depression.

AIM: To investigate the influence of adenosine on human umbilical vein endothelial cells (HUVEC) bFGF protein production and bFGF mRNA expression. METHODS: Immunohistochemistry staining was performed to detect bFGF protein. RT-PCR was performed to detect bFGF mRNA expression. RESULTS: Immunohistochemistry study demonstrated that there was only a small amount of bFGF positive cells and the color was weak in control group (without adenosine). In groups treated with 10~-4 mol/L and 10~-6 mol/L adenosine, bFGF protein was significantly higher than that in control group (P0.05). RT-PCR showed that in 10~-4 mol/L and 10~-6 mol/L adenosine groups, bFGF mRNA expression was higher than that in control group (P0.05). CONCLUSION: Adenosine may promote HUVEC proliferation and angiogenesis partly through inducing bFGF expression.

Giving up treatment in ophthalmology is common in our country. Financial difficulty is the main reason of abandoning treatment in ophthalmology. We should make every effort to deal with some patients with ocular diseases who had no light perception at all. Misdiagnosis and wrong therapy ought to be avoided as much as possible in our clinical practice. The giving up treatment of retinoblastoma is relatively common in our country at present time and its early diagnosis and early treatment is very important. Therapeutic giving up treatment is a new concept and eye doctors can make use of it in some special situations in that the disease can be treated effectively.

Objective To observe the clinical manifestation and ophthalmoscopic image characteristics of uveal metastatic carcinoma.Methods Thirty-six uveal metastatic carcinoma patients (43 eyes) were enrolled in this study.The patients included 21 males and 15 females.The patients' ages ranged from 28 to 71 years,with a mean age of (47.3 ±10.2) years.Seven patients had bilateral carcinoma and 29 patients had unilateral carcinoma.There were 30 patients with lung cancer,three patients with breast cancer,one patient with gastric cancer and two patients without primary tumors.There were 20 patients with known primary cancer,16 patients visited the Department of Ophthalmology first.All the patients were examined documenting visual acuity,intraocular pressure,slit-lamp microscopy and mydriatic fundus examination.Meanwhile,22 patients (26 eyes) were examined using B-type ultrasound and/or color Doppler flow imaging(CDFI).Twelve patients (12 eyes) were examined using fundus fluorescein angiography (FFA) and/or indocyanine green angiography (ICGA).Seventeen patients (22 eyes) were examined using MRI and/or CT.The clinical manifestation and ophthalmoscopic image characteristics of uveal metastatic carcinoma patients were observed.Results Among 43 eyes,four lesions were in the iris,three lesions in the ciliary body and 32 lesions were in the choroid.Fundus examination showed an isolated mass in 26 eyes and more than two masses in nine eyes.Metastatic tumors of the iris and ciliary body often showed irregular cauliflower-like mass with gray-white or meat-red color and abundant vessels.The choroidal metastasis usually demonstrated flat rounded or irregular intraocular masses with gray-yellow or gray-white color in the posterior pole.B-type ultrasound showed ill-defined,flat,and irregular-shaped masses with uneven internal reflectivity.CDFI showed rich blood flow within the tumor.FFA and (or)ICGA showed pinpoint and mottled leaks against hyperfluorescence background.MRI revealed low or middle signal using T1 WI and low signal intensity using T2WI.Conclusions The uveal metastatic carcinoma usually occurs in one eye with an isolated mass.Most of them show a flat gray-yellow mass in posterior choroids and have the primary cancer sites of the lung.FFA and/or ICGA show pinpoint and mottled leaks against hyperfluorescence background.B-type ultrasound and (or) CDFI show ill-defined,flat,and irregular-shaped mass with rich blood flow within the tumor.MRI reveals low signal intensity on T2 WI.

OBJECTIVE To investigate the infection status and drug resistance of Ureaplasma urealyticum (Uu) and Mycoplasma hominis (Mh) isolated from female genitourinary tract. METHODS The specimens of 1890 cases were collected from Jan 2003 to Dec 2006. The results of mycoplasma cultivation and susceptibility test were analyzed retrospectively. RESULTS Among 1890 cases, there were 756 cases with positive mycoplasma cultivation. Positive rate was 40.0%. Positive rate was the highest at the age of 21 to 40. Among 11 commonly used antibiotics, sensitivity of tetracyclines was the highest,followed by macrolides. Four quinolones all showed the higher resistance. CONCLUSIONS The resistance of commonly used antibiotics in mycoplasma isolated from female genitourinary tract is greatly severe.We should think highly of it.

Objective To explore the protective effects of extraction of Glycyrrhiza uralensis Fisch. (GC) on CCl4 induced acute hepatic injury in mice. Methods Acute hepatic injury model was made by CCl4. Fifty mice were divided into 5 groups (normal control group, CCl4 model group, bifendate group, low and high dose of GC group) and administrated for 7 days. The levels of ALT, AST, ALP, LPH, TP, ALB, GLB in serum and the activities of SOD, MDA in liver tissue were detected. Results The activities of ALT, AST and the level of MDA in GC group were significantly decreased, and the activity of SOD were significantly increased compared with the model group (P

Objective To study and solve the repairing and reconstruction characteristic for chronic radioactivity ulceration on chest wall.Methods Using transverse rectus abdominis musculocutaneous flaps (TRAM) to repair and reconstruct 12 cases serious chronic radioactivity ulceration after radical operation of mastocarcinoma.Including 6 cases using single pedicle TRAM flaps, 6 cases using double pedicle TRAM flaps.Results All 12 cases were applied successfully with 100% survived and were followed-up for 1 to 4 years.There were better colour, texture, elasticity on flap and obviously improvement for cicatricial tissue round ulceration.Conclusion It is better choice for repairing chronic radioactivity ulceration on chest wall with transverse rectus abdominis musculocutaneous flaps and it is also reliable method for flap circulation using double pedicle TRAM flaps by vascular anastomosis.

Objective To investigate the effects of the therapy of invigorating the kidney and qi and dissipating blood stasis to removing the toxin on the hepatocyte apoptosis and associated genes in the liver fibrosis rats,and to explore the treatment mechanism of liver fibrosis with TCM.Methods liver fibrosis model rats were induced by CCl4,and the Chinese herbs(Ganlike)with invigorating the kidney and qi and dissipating blood stasis to removing the toxin was given by daily gavage,after the experiment,the pathological changes of liver were observed with optical microscope.Hepatocyte apoptosis was detected with TUNEL assay,apoptosis-associated genes were detected with immunohistochemistry assay.Results The Chinese herbs Ganlike could obvously improve the pathological changes,alleviate the hepatocyte apoptosis and modulate the expression of apoptosis-associated genes.Conclusions The anti-fibrosis mechanism of Ganlike may be to regulate the hepatocyte apoptosis of liver fibrosis rats and to restrain the expressions of hepatocyte Fas/FasL,Bcl-2/Bax.

Objective To study the interactive effects of fluorine,calcium and iodine on thyroid SOD,CAT and MDA of rats.Methods Based on 2?2?2 factorial design,80 Wistar rats were randomly divided into 8 groups and diet with different ingredients: ① moderate concentrations of fluorine(F),calcium(Ca) and iodine(I)(NF,NCa,NI);② moderate concentrations of F,I and high concentration of Ca(NF,NI,HCa);③ moderate concentrations of Ca and I and high concentration of F(NCa,NI,HF);④ moderate concentration of I and high concentrations of F and Ca(NI,HF,HCa);⑤ moderate concentrations of F and Ca and low concentration of I(NF,NCa,LI);⑥ moderate concentration of F,high concentration of Ca and low concentration of I(NF,HCa,LI);⑦ moderate concentration of Ca,high concentration of F and low concentration of I(NCa,HF,LI);and ⑧ high concentrations of F and Ca and low concentration of I(HF,HCa,LI).The moderate concentration of F was 90?g/d,and the high concentration of F was 30 times that of moderate concentration.The moderate concentration of Ca was 13mg/d,the high concentration of Ca was 20 times that of moderate concentration.The moderate concentration of I was 3.5?g/d,i.e.15 times that of low concentration.After six months,the activities of SOD and CAT,and the content of MDA in the thyroid were determined by xanthine oxidase method,visible spectrophotometry and colorimetry.Results According to the results of factorial ANOVA,the significant interaction of F with I on SOD was found(F=3.97,P=0.05).The activity of SOD in ⑤ group was significantly higher than that in ① group(t=2.36,P=0.03).The activity of SOD in ⑥ group was higher than that in ② group(t=-3.04,P=0.01).The activity of SOD in ⑧ group was lower than that in ⑦ group(t=2.36,P=0.03).The activity of SOD in ④ group was higher than that in ② group(t=-2.91,P=0.01).A significant interaction of fluorine,calcium with iodine to CAT was found(P

Objective To investigate the curative effect and influential factors of small hepatocellular carcinoma (SHCC) treated by interventional therapy. Methods 42 patients with SHCC were treated by interventional therapy. Some possible prognostic factors were analysed with COX proportional hazard regression model. Results (1)Hepatic arteriographic manifestations: 26 of 42 cases SHCC revealed rich tumor vessels, 37 had tumor stain including 34 with nodular stain. (2)The overall 1 , 3 , 5 year survival rates of SHCC using Kaplan Meier method were 88%(37/42),74%(31/42),51%(21/42), respectively. (3)Clinical staging and typing of the patients,and accumulation of Lipiodol within the tumor obviously influenced interventional effects of SHCC. Conclusion (1) The most important diagnostic angiographic sign of SHCC is tumor staining, besides tumor vessels. (2) Interventional therapy of SHCC has an ideal effect and should be an important nonoperable curative method.