Objective To observe the effect of Maiya on the hormone level of the female mice. Methods Female mature mice (5 ～ 6 months) were ovariectomized and treated with Maiya. Follicular stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2) and progesterone (P) in blood were examinated. Results Maiya could increase the FSH and E2 in blood, and decrease P in blood of the castrated mice, while the level of LH had no obvious change. Conclusion Maiya can influence the level of hormone.

Objective:To study the radiation dose rate around patients underwent renal dynamic imaging of SPECT with 99Tcm-DTPA, so as to provide the basis for ensuring the radiation safety of the surrounding environment and the general public.Methods: 109 patients who underwent renal dynamic imaging were enrolled in this study, and the radiation dose rates of different time and different distance after 99Tcm-DTPA was injected patients were measured. And then, the changes of dose rate following time and distance were analyzed. Results: At the 0.5h, 1h, 2h, 3h, 6h, and 7h after radiopharmaceuticals were injected patients underwent renal dynamic imaging, the radiation dose rates were 0.76-8.86 μSv/h, 0.68-7.27 μSv/h, 0.57-4.52 μSv/h, 0.56-3.90 μSv/h, 0.23-2.07 μSv/h and 0.21-1.05 μSv/h, respectively, between 0.5 m and 4 m away from patients. At 24h after radiopharmaceuticals were injected, all of radiation dose rates at different distances around patients were same with background values.Conclusion: The radiation dose rates around patients underwent renal dynamic imaging will rapidly decrease with the increasing of time and distance. At 24h after radiopharmaceuticals were injected, all of radiation dose rates at different distances away from patients were same with background values.

4cm) or involved the urethra、 vagina、 clitoris or anus. Pathologic grouping was 25 squamous carcinomas 3 adenocarcinomas and 2 undifferentiated carcinomas. Radiotherapy was given by angled radiation with wedge block. Dose was 65 to 70 Gy/6-7W. RESULTS Thirty patients completed their inductive chemotherapy within 30 days for 86 courses. The response rate was 86. 7% (26/30). CR was 30. 16%. 18 of 30 patients were followed-up for over 5 years. Five years survival rate was 72.2 (13/18). Major acute complications were vuvitis (23%). Late adverse effects were 1 patient with ure-throstenotic uroschesis, 1 patient with pathologic transcervical fracture. None of patients had radiation rectitis and cystitis. CONCLUSION Inductive chemotherapy consisting of modified VBP regimen was active in reducing the vulvar tumor volume. Radiotherapy used angled radiation with wedged block to treat advance vulvar cancer was fa-ciliated. Chemoradiotherapy was found to be an effective treatment for locally advanced vulvar carcinoma, with acceptable morbidity even in an elderly population. The technique allowed maintenance of normal anatomical and physiologic function that would have been compromised by primary surgical approach. It yielded survival rates comparable to those achieved with ultraradical surgery in younger patients. Life quality was improved significant-ly.

Objective To evaluate the clinical effect in patients with primary liver cancer (PLC) well filled with Lipiodol after transcatheter arterial chemoembolization (TACE). Methods 144 patients with PLC well filled with lipiodol after TACE were analysed retrospectively, and the survival rates were calculated by Kaplan Meier method. Results The overall cumulative survival rates for 1,2,3,4 and 5 year were 85.0%, 63.7%, 51.7%, 33.9% and 16.9% respectively. Significant differences were found for 1,2,3 and 4 year survival rates and that of 400 patients with PLC treated by TACE reported previously by our department ( P

Objective To explore possible physiological and biochemical mechanisms of Lagenidium giganteum infection in killing mosquito larvae. Methods The content of lipid and the activities of esterase and lipase between the normal and infected mosquito larvae were observed with histochemical method. The results were photomicrographed and analysed by image analysis using computer. Results In 24 hrs after infection, the content of lipid in the infected Culex quinquefasciatus larvae was lower while the activities of esterase and lipase in the larvae were higher than the control. In 48 hrs and 78 hrs after infection, the content of lipid in the infected larvae of C. quinquefasciatus and Ae. albopictus were significantly lower while the activities of esterase and lipase were significantly higher than those of control. Conclusion The content of lipid in the mosquito larvae decreased while the activities of esterase and lipase increased after the infection of Lagenidium giganteum . The disorder of lipid metabolism might be part of the killing mechanisms to C. quinquefasciatus and Ae. albopictus larvae.

Objective To evaluate the effects of the laryngeal mask airway for spontaneous breath-ing in short pediatric operation under Sevoflurane inhalation anesthesia.Methods Eighty children undergo-ing hernia surgery, of whom ASA Ⅰ or Ⅱ, in accordance with the random number table, were randomly divided into two groups( each 40 cases):the laryngeal mask airway for spontaneous breathing with Sevoflu-rane inhalation anesthesia in group A,and tracheal intubation for controlled ventilation with Sevoflurane inha-lationa nesthesia in group B.Children in the two groups were fasting for 6 hours and without drinking for 4 hours before anesthse ia.Anestheis a was induced with Sevoflurane inhalation fro all patients.The laryngela mask airway was insertde soon afet r induction of anesthesia with Sevoflurane inhalation,and anse thesia main-tained with Sevoflurane in halta ion in gor up A.P atients in gruo p B wereg iven cisatracurium 0.15 mg/kg intravenous injectio n before tracheal intubation, and anesthesia maintained with Sevoflurane inhalation, 40 minutes before the end of the surgeryt o sot p using muscle relaxant.Ten minutes before the end of the operation to stop Sevoflurane inhalation,in two groups.The end-tidal carbon dioxide partial pressure(PETCO2 ), mean blood pressure,heart rate,respiratory rate,pulse oxygen saturation and end-tidal sevoflurane partial pres-sure(PETSev) were recorded one minute before the induction of anesthesia,15 min,30 min,45 min,and 60 min after the induction of anesthesia.Both the time to regain consciousness and the time to remove laryngeal mask airway or tracheal catheter were recorded in two groups.Results The mean blood pressure, heart rate decreased in two groups after the induction of anesthesia(P<0.05).The value of PaCO2 increased and that of base excess decreased in 30 min after the induction of anesthesia in group A(P<0.05).The value of PETCO2 increased, and that of pH decreased in 45 min after the induction of anesthesia in group A (P<0.05).The movements of the limbs and head induced by operative stimulation in group A were more than those in group B(P<0.05),but the incidences of cough and laryngospasm induced by removing the laryngeal mask airw ay or tracheal catheter in group B were higher than those in group A(P<0.05).Both the time to regain consciousness and the time to remove laryngeal mask airway or tracheal catheter were shorter in group A than those in group B ( P<0.05 ) .Conclusion It is safe that the laryngeal mask airway for spontaneous breathing with Sevoflurane inhalation anesthesia in short pediatric operation.The time of anesthe-sia is beyond half an hour during operation and the artificial respiration sholu d be implemented.

Objective To Evaluate the environment quality in underground construction site based on the measurement of atmosphere composition and microclimate. Method Concentrations and composition of gases were analyzed by combined detection with mass spectrometer and gas chromatograph. Microbial content was detected by striking method. A microclimate model was built based on the measurements of temperature, humidity and wind speed in two years. Result Concentrations of inorganic gas was higher than national standard. Total volatile organic compounds were in the range of 4～6 mg/m3, which highly exceeded the national standard. Concentration of O2 descended obviously at the time when personnel went into the construction site, and was lower than the national standard of 299 mg/m3 after continuous working for 3 h. When an aerator was started, the average noise level was in the range of 70～93 dB, which was higher than the national standard(55～70 dB). In the course of working, the average microbial content reached 4 000 cfu/m3 which was 3 times as high as the background level. The microclimate characteristic with high humidity, low temperature and inadequate sunshine was unsuitable for dwelling. Conclusion Environment in the underground construction site is unfavorable as compared with that on the ground. The physiological function of working staff may be influenced obviously due to cumulative effect of the above mentioned factors, and may lead to aprosexia, hypomnesis, fatigue, etc. Six kinds of protection means are suggested in the paper.

Objective　The covered tracheal stent in shape of a straight tube or a fork-like tube was placed clinically to treat tracheal stricture and tracheal fistula caused by malignant tumour in order to maintain the normal function respiration and to keep the fistula closed.Methods　The stent was made of stainless steel in shape of "Z"covered with membrane partially or entirely.An instrument with tube in three sheathes was used to place the stent in position.Results　The tracheal stents were placed successfully in 9 cases at first time and the esophageal stents were placed for 6 cases among them in the meantime.Dyspnea of patiants was remarkably alleviated.The tracheal fistula of them was closed effectivelly.Follow-up for 4～12 monthes all cases demonstrated no adverse effects.Conclusion　The application of the covered stent is a safe effective method to treat the tracheal stricture and tracheal fistula.

BACKGROUND: Mesenchymal stem cells have ability of multi-directional differentiation, and can be induced to differentiate into epithelial cells in vitro. The differentiation of epithelial cells in fetal primitive gut is induced by mesochymal cells of intestines. The report of bone marrow mesenchymal stem cells (BMSCs)differentiate into epithelial cells induced by intestinal connective tissue has not been identified. OBJECTIVE: To observe the possibility of BMSCs differentiate into epithelial cells induced by fetal intestinal connective tissue. DESIGN: Culture in "vitro and comparative observation. SETTING: The experiment was carried out in the Department of Histology and Embryology, Chongqing Medical University from July 2004 to July 2006. MATERIALS: Epidermal growth factor (EGF, Sigma); CK, CK20 (Zhongshan Bio-Tech, Co.,Ltd). Bone marrow of limbs was collected from 6 aborted fetus samples aged 4-5 months. Adding Ficoll to centrifugalize, BMSCs were isolated, cultured and proliferated. The intestinal segment about 15 mmx5 mm was obtained sterilely from fetal duodenal papilla to colon, then muscular tunic and adventitia were peeled. Enzymatic digestion was used to remove the epithelial cells on the mucosa surface. The lump of intestinal connective tissue was cut into 15 minx5 nun. Fetus samples were provided by Department of Gynaecology and Obstetrics in Clinical College, all the parturients agreed to the offer, and the experiment was approved by the hospital ethical committee. METHODS: The experiment was assigned into 4 groups. In groups A and B, the DAPI labelled BMSCs (3x104) at the third generation were transplanted on the submucosa of intestinal connective tissue;.In groups C and D, the DAPI labelled BMSCs were only cultured on the cover glass; In groups B and D, EGF in final concentration of 10 ng/mL was added. MAIN OUTCOME MEASURES: After cultured for 12 days, the morphous and distribution of DAPI labelled BMSCs were observed under fluorescence microscope; the cell morphous on surface of the same intestinal connective tissue was observed with hematoxylin-eosin stain. Expressions of CK and CK20 as well as whether the intestinal connective tissue express EGF were all detected by immnnohistochemical stain. Production of basal lamina between the DAPI labelled cells and connective tissue was assayed with periodic acid-Schiff reaction.RESULTS: On the surface of the tissue lump in groups A and B, the DAPI labelled BMSCs could be seen under fluorescenc microscope, in the same section stained by hematoxylin-eosin, there were some epithelioid cells. During culture, the medium were changed several times, the marked cells that were not eluted, grew on the surface of connective tissue.Immunohistochemistry revealed, in groups A and B, cells on the surface of the tissue lump both expressed CK and CK20. Immunofluorescence stain shown that some of cells on the tissue lump had the DAPI marked nncelus (sapphirine fluorescence) and CK (red fluorescence) in cytoplasm simultaneously. It indicated that the 3rd generation of DAPI labelled BMSCs had differentiated into epithelial cells. Cells in group C were negative for CK and CK20, but those in group D were positive, which indicated that EGF could induce BMSCs differentiate into epithelial cells. There were glycosidoprotein with basal membrane-like structures appeared between the cells and connective tissues in periodic acid-schiff stained section. The uncultured connective tissue expressed EGF. CONCLUSION: Both EGF and fetal intestinal connective tissues can induce BMSCs differentiate to epithelial cells in vitro; the EGF presented in the intestinal connective tissues may be one of factors in this induction process.

Objective To explore the value of the application of vascular enhancement technology (VET) in the diagnosis of Budd-Chiari syndrome (BCS) and the evaluation of the therapeutic efficacy of interventional therapy.Methods B-mode ultrasound,color Doppler and VET were performed on 93 patients with BCS for the systematic detection of intrahepatic vessels and the inferior vena cava (IVC),with the static and dynamic images of these vessels stored prior to interventional therapy.Subsequent to the operation,the same procedures were repeated on the vessels concerned and the images were recorded.The definitions of B-mode and VET images of the detected vessels were compared by two sonography experts.Regarding DSA as standard,the diagnostic accordance rate of VET and B-mode imaging of the diseased vessels was compared.Results Of the 613 vessels detected prior to intervention therapy,440 vessels images were distinct by B-mode and 533 by VET(P<0.05).VET demonstrated 37 intrahepatic collaterals which were invisible by B-mode.Regarding DSA as the gold standard,the diagnostic accordance rate of affected vessels of B-mode and VET was 69.2% and 92.5%,respectively(P<0.05).In total 103 therapeutic vessels,B-mode sonography and VET revealed 81 and 95 distinct vessels,respectively(P<0.05).Conclusions VET can improve the definition of veins and venous stents.The combination of VET and color Doppler can improve the clinical value of ultrasound in the diagnosis of BCS and evaluation of the therapeutic efficacy of interventional therapy.