Objective To investigate the renoprotective effect of reduced glutathione on streptozotocin-induced diabetic rats and its possible mechanism.Methods Streptozotocin-induced diabetic rats received aminoguanidine(50 mg?kg~(-1)?d~(-1)) and reduced glutathione 400 mg?kg~(-1)?d~(-1) intraperitoneally respectively or synchronously for(8 weeks.) The expression of TGF-?1 mRNA and protein in renal cortex were determined by reverse transcription polymerase chain reaction(RT-PCR) and immunohistochemistry respectively.The mean glomerular area(MGA) and volume(MGV) were measured by image analysis system.The changes of creatinine clearance rate(Ccr),the kidney weight/body weight ratio and the urinary albumin excretion rate(UAER) were determined.Results By the end of 8 weeks,the Ccr,UAER,MGA,MGV,kidney weight/body weight ratio,the contents of TGF-?1 mRNA in renal cortex were increased significantly in DM groups compared with the blank control group(P

Objective To explore the clinical correlation of coronary heart disease with diabetes.Method From January 2005 to December 2008,268 patients were enrolled and randomly divided into the study group(Coronary Heart Disease combined with Diabetes)and the control group(Coronary Heart Disease).Blood pressure,hyperlipemia,degree of vasculopathy and chief heart incident was compared.Result The rate of blood pressure,hyperlipemia and chief heart incident in the control group were significantly lower than the study group(P＜0.05).The incidence rates of multi-branches lesions in the control group were significantly lower than the study group(P＜0.05).The incidence rates of single-branches lesions in the study group were significantly lower than the control group(P＜0.05).Conclusion Active measures should be taken to prevent diabetes and control the risk factors like blood sugar,blood fat,blood pressure,weight and so on in order to prevent the attack of coronary artery diseases.

Hypoxia-inducible factor(HIF)is a pivotal transcription factor for hypoxic response.This article reviews the related characteristics of HIF-1 and its target genes vascular endothelial growth factor and the neuroprotective mechanisrms in cerebral ischemia.

Objective To explore the application and formulas of the tumescent technique in the ultrosonic liposuction for better and more predictable reseults. Methods The tumescent technique formulas were present and analysed through 318 cases of ultrosonic liposuction. Results The following regimen was recommended as a result of the above studies: Lactated Ringer's solution or nosmal sodium was the fluid of choice; Lidocaine (500 mg/l) was a reference anesthetic. The maximal safe dose recommended for liposuction was 35 mg/kg body weight and total dose of epinephrine was 2～3 mg/l. But its concentation should be lower than 1:1 000 000. Concentration of sodium bicarbonate was 500 mg/l, and higer doses were of no value but harmless. Conclusions Safer formulas are provided for the tumescent technique administration in the ultrosonic liposuction. The tumescent technique may reduce potential complications related to ultrosonic liposuction

Objective To discuss the benefits of the external ultrasonic liposuction through comparing the different effects of external ultrasonic tumescent liposuction and simple negative pressure liposuction. Methods The clinical effects of ten patients were compared, in which one side of abdomen was treated with external ultrasonic tumescent liposuction, and the other side with tumescent liposuction and the aspirates were analyzed. Results The percentage of supernatant fat in the whole aspirated volume on the ultrasonic treated sides was higher than that of non ultrasonic treated sides ( P

Objective To evaluate the efficacy of transcatheter lipiodol chemoembolization therapy (TOCE) for HCC via inferior phrenic artery (IPA) and to analyse the location of the tumor feeding inferior phrenic artey. Methods Twenty five cases of HCC underwent the procedure of TOCE via the IPA, as well as the hepatic artery using Seldinger's method. The patterns of tumor growth included huge type in 12 cases, solitary nodular type in 8 cases and multiple nodular type in 5 cases. Hepatic artery and inferior phrenic artery chemoembolizations were performed in all cases. Results Inferior phrenic artery originated from celiac trunk in 16 cases (64%); abdomen aorta around celiac trunck in 8(32%). The sitesot tumors supplied by IPA in right lobe (Ⅶ,Ⅷ segment) were 23 cases and left lobe (Ⅳ segment) 2 cases. The cumulative survival rates of IPA chemoembolization for hepatocellular carcinoma were 84%(1 year) and 68%(2 years). No severe complications occurred. Conclusions TOCE of the IPA is a safe and effective method in the management of HCC supplied by IPA. When the tumor site is adjacent to diaphragm, hepatic ligaments or bare area, may arouse the blood supply by IPA, especially in no tumor staining or staining defect in hepatic artery angiography but tumor enhancement on CT, and increase of the level of serum ? fetoprotein.

Objective To determine the clinical efficacy and safety of domestic biliary metallic stents in the management of biliary obstruction. Methods 75 domestic biliary metallic stents were implanted in 66 of 72 patients with obstructive jaundice due to malignant (n=70) and benign stenoses (n=2). Results The technical successful rate was 92.7% (64/66). Palliation with rid of jaundice was achieved in 96.9% (64/66) within 1 month. The 30-day mortality rate was 3% (2/66). Early complications occurred in 7.6% (5/66). 44 patients were followed from 2 to 24 months after treatment. 33 (33/42) of 42 patients with malignancy died after a median survival of 5.3 months (range 2-23 months). The remaining 9 patients (9/42) remained alive with a mean follow-up of 11.6 months. The stent patency rates were 92.8%,89.9% and 79.4% at 3,6,12 months respectively. 6 patients (6/42) presented stent occlusion within a median period of 3 months. One of two patients with benign stenoses sustained a patency duration time of 24 months. One patient died 3 months after treatment. Conclusions The domestic biliary metallic stent implantation is efficacy and safety in the management of biliary obstruction.

Objective To investigate the role of receptor for advanced glycation end products (RAGE)/NF-κB signaling pathway in mediating lysophosphatidylcholine (LPC)-induced TGF-β1 expression in human retinal endothelial progenitor cells (HEPCs).Methods Human retinal endothelial cells (HERCs)were transfected with siRNA for RAGE siRNA or added NF-κB in-hibitor pyrrolidine dithiocarbamate (PDTC)in the presence or absence of LPC,the expressions of TGF-β1 and RAGE genes were analyzed by qPCR and Western blot.Results LPC could increase the expression of RAGE and TGF-β1 gene in HERCs.The RAGE gene after silence could significantly decrease the expression of LPC-induced RAGE and TGF-β1 .Adding NF-κB inhibitor PDTC significantly reduced LPC-induced RAGE and TGF-β1 expression in HERCs.Conclusion RAGE/NF-κB signaling pathway plays an important role in mediating LPC induced TGF-β1 gene expression in HERCs.

Objective To evaluate the value of the neutrophil-lymphocyte ratio (NLR) in elderly type 2 diabetic patients (T2DM) with coronary heart disease (CHD).Methods We performed a retrospective observational study on 228 patients undergoing coronary angiography in Guizhou Provincial People's Hospital from April 2014 to July 2015.Patients were divided into three groups:the simple T2DM group (n=77),simple CHD group (n=72),and T2DM complicated with CHD group (n=79).Meanwhile,70 healthy elderly subjects served as the control group.The white blood cell count,high-sensitivity C-reactive protein (hs-CRP) and other clinical and laboratory parameters were collected,and NLR was calculated.Risk factors for CHD in T2DM patients were determined by logistic regression analysis.Multiple stepwise regression analysis was adopted to identify factors influencing NLR.Results The white blood cell count,neutrophil count,NLR and hs-CRP level in the simple T2DM,CHD,and T2DM+CHD groups were higher than in the control group [(7.48 1.81) 109/L,(7.72± 1.89) 109/L,(7.98±2.12) 109/L vs.(6.22± 1.61) 109/L;(4.49±1.38) 109/L,(4.88±1.56) 109/L,(5.35±1.40) 109/L vs.(3.52±0.84) 109/L;(2.84± 0.77),(3.07±0.79),(3.34±0.83) vs.(1.58±0.42);(2.92±0.65) mg/L,(3.20±0.86) mg/ L,(4.98±1.10) mg/L vs.(1.105±0.23) mg/L;respectively,P＜0.05 or P＜0.01].The lymphocyte count in the simple T2DM,CHD,and T2DM+CHD groups were lower than in the control group [(1.57±0.41) × 109/L,(1.58±0.40) × 109/L,(1.61±0.48) × 109/L vs.(2.22± 0.51) × 109/L,P＜0.05].NLR and hs-CRP levels in the T2DM+CHD group were higher than in the former two groups (all P＜0.05).Pearson correlation analysis showed that NLR was positively correlated with the Gensini score and hs-CRP level (r=0.7455 and 0.7792,both P＜0.01).Logistic regression analysis showed that NLR,hs-CRP levels and glycosylated hemoglobin A1c (HbA1c) were the risk factors for CHD in T2DM patients (OR=4.331,3.997 and 2.928,all P＜0.05).Multiple stepwise regression analysis showed that NLR was positively correlated with fasting plasma glucose,HbA1 c levels and systolic blood pressure (β' =0.3133,0.4720 and 0.3069,all P＜0.05).Conclusions NLR may be a valuable predictive factor for CHD in elderly T2DM patients.

BACKGROUND:Neural stem cel transplantation has been used to treat a series of brain injury diseases, such as cerebral palsy, but its effect on Alzheimer’s disease is rarely reported. OBJECTIVE:To observe the effect of neural stem cel transplantation on the behavior and immune regulating system of Alzheimer’s disease rats. METHODS:Thirty-five Sprague-Dawley rats were enrol ed to make a postcerebral incision and given hippocampal injection of amanita phal oides acid to establish rat models of Alzheimer’s disease. Another 10 rats were only given hippocampal injection of normal saline after preparation of postcerebral skin incision as sham operation group. Then 32 successful rat models were randomly divided into two groups (n=16 per group):rats in experimental group were administrated hippocamal injection of 5×109/L al ogeneic neural stem cel suspension;those in model group were given no injection. Five-day Morris water maze test was conducted at 4 weeks after transplantation. At 1 week after Morris water maze test, levels of interleukin-1 and interleukin-10 in the cerebral homogenate were detected, as wel as pathological changes of brain tissues were observed in the three groups. RESULTS AND CONCLUSION:Compared with the model group, the abilities of cognition and memory were significantly higher in the sham operation group (P<0.01), and the abilities of spatial learning and memory were significantly higher in the experimental group (P<0.05, P<0.01). Levels of interleukin-1 and interleukin-10 in the model group were significantly higher than those in the sham operation group (P<0.01) but significantly lower than those in the experimental group ( P<0.01). Besides, the number of neurons in the model group was obviously less than that in the experimental and sham operation group. These results indicate that neural stem cell transplantation supplements and protects neurons against Alzheimer's disease in rats, thereby significantly improving the learning and memory ability.