Whole-body PET/CT scanning is accompanied by substantial radiation dose in clinic. A large proportion of the radiation dose for examination obtained from whole-boby PET/CT is from CT scan. So how to control radiation dose without impairing CT image quality determined by the clinic is particularly important. Anthropomorphic phantom is the best choice for the study of radiation dose and image quality in PET/CT. The article reviews the meaning of anthropomorphic phantom and the study about PET/CT image quality and radiation dose with the phantom, Then analyzes the relationship between image quality of CT in PET/CT and radiation dose in the practical application.

BACKGROUND: Magnetic labeling of stem cells is a recently developed stem cell in vitro labeling technique. Through in conjunction with magnetic resonance imaging (MRI), it can monitor transplanted stem cells in vivo. OBJECTIVE: To identify the method of superparamagnetic iron oxide (SPIO) labeling pig bone marrow mesenchymal stem cells (BMSCs), to investigate the characteristics of stem cells labeled by various SPIO following MRI, and to determine the minimum amount of labeled cells fer MRI. DESIGN, TIME AND SETTING: A control observation was performed at the laboratories of Department of Cardiovascular Surgery, Medical College of Soochow University, and Medical Imaging Centre, First Affiliated Hospital, Soochow University between September 2006 and March 2007. MATERIALS: Fresh porcine iliac bone marrow was collected from Taihu Meishan pigs. SPIO nanometer particles were purchased from Schedng, Germany. Ultramicro SPIO (USPIO) nanoparticles were provided by School of Chemistry and Chemical Engineering, Soochow University. For such particles, crystal nucleus surface was coated with dextran, and following coating, they were named 1#, 2#, 3# for short according to particle size. METHODS: Following isolation, purification, and culture, BMSCs were in vitro labeled by various kinds of SPIO nanoparticles. The labeled cells were subjected to Prussian blue staining and fluorescence microscope observation. The cell growth was observed using MTT method and the growth curve was plotted. For Feridex-labeled cells, 1×106), 5×105, and 1×105 three cell amount groups were set, for unlabeled cells, a 5×105 cell amount group was included, and for 1#, 2#, and 3# SPIO-labeled cells, only 5×105 cell amount group was used. MRI was conduced for measurement of signal intensity of cells labeled by different scanning sequences, followed by statistical analysis. MAIN OUTCOME MEASURES: Detection of SPIO nandparticles-labeled cells by Prussian blue staining; Growth curves of SPIO nanoparticles-labeled cells; Detection of cellular apoptosis by double staining; Determination of signal intensity of cell masses from different Ependoff tubes using MRI with T1WI, T2WI, and fast field echo (FFE) sequences. RESULTS: BMSCs could be labeled with SPIO and the labeling efficiency was 100%. Different amounts of blue-stained Fe particles could be observed in the cytoplasm by Prussian blue staining. SPIO labeling caused a significantly lower signal attenuation effect in T2WI and FFE (T2*WI) images than in T1WI images. In a labeling concentration of 25 mg/L Fe solution, the minimum cell amount for MRI was 1 x 105. The signal intensity exhibited significant difference in 2#, 3#USPIO- and Feridex-labeled cells in no matter T2WI or T2*Wl images(P < 0.01). But no significant difference was found between 1#USPIO- and Feridex-labeled cellss in no mater T2WI or T2*WI images(P > 0.05). There was significant difference in signal intensity of Feridex-labeled BMSCs between T2WI, T2*WI and T1Wl images (P < 0.01). CONCLUSION: BMSCs can be easily and efficiently labeled by SPIO nanoparticles without interference, at proper concentrations on cell viability and proliferation. MRI visualization of SPIO-labeled BMSCs is feasible in both T2WI and T2*WI images.

Objective To explore baccalaureate nursing students' perception of caring, so as to provide evidence for promoting their caring behaviors. Methods Phenomenology was the chosen methodology, 11 baccalaureate nursing students were interviewed in depth. Results Themes of caring were formed into four categories: caring behavior was reciprocal; the motives of caring included fear, concern, responsibility and obligation; knowledge, technical competency and professional helping relationship constituted caring; the confusions of caring came from many factors in clinical practice. Conclusions Baccalaureate nursing students can understand the connotation of caring accurately. To encourage their caring behaviors,clinical supervisors and managers should take all kinds of measures to maintain their faith in caring.

Objective To explore the change of glycogen synthase kinase-3(GSK-3) activity in lymphocytes in patients with mild cognition impairment(MCI).Methods The phosphorylation of GSK-3? at Ser 9 site and total level of GSK-3? in peripheral blood were measured by Western-blot in normal control group,one-year MCI group,two-year MCI group and three-year MCI group.Results The phosphorylation of GSK-3? at Ser 9 site were lower in 1-year MCI group,2-year MCI group and 3-year MCI group,compared with normal control group(all P0.05).Conclusion The activity of GSK-3 in lymphocytes in MCI patients is raised,which implies a subsidiary value of MCI diagnosis.

Objective To improve the freeze-dryer shelf temperature control effect.Methods The fuzzy-PID control algorithm that's adjusting the PID parameters in real-time in the control process,was adopted to control the shelf temperature,expecting to adapt the changing of process parameters of shelf.Results In the process of freeze-drying temperature control shelves,the shelf temperature static performance and dynamic performance were improved,and the effect of shelf temperature control was archived satisfyingly.Conclusion In the freeze-drying process,the process parameters of shelf are changed along with the drying time and the working environment.The conventional PID control is difficult to obtain satisfactory results.The fuzzy-PID controller,which can adjust PID parameters on real-time in the controlling process,be used in the shelf temperature control,and the result is satisfied.

Objective To study the effect of evodiamine(EVO)-stimulated dendritic cells(DC) of spinal cord homogenate protein(hpDC) on repair of spinal cord injury(SCI) in mice.Methods Twenty hours after SCI,mice were randomly divided into 6 groups and injected with PBS,EVO,DC,DC+EVO,hpDC and hpDC+EVO.BMS and histopathology were used to observe the effect of hpDC+EVO on the functional recovery of spinal cord injury and pathological changes such as local scars.Levels of brain-derived neurotrophic factor(BDNF),neurotrophin-3(NT-3),interleukin-12(IL-12) and granulocyte macrophage colony stimulating factor(GM-CSF) in injured sites and supernatant of cultured T cells were measured by ELISA.Results Eighty-four days after treatment,the BMS was significantly higher in hpDC + EVO group than in other groups(P

AIM: To investigate the relaxative characteristics of resveratrol(RES) and 17?-Estradiol(EST) on coronary arteries in dogs and its mechanism.METHODS: Rings of canine coronary arteries were suspended in organ baths containing Krebs-Henseleit solution,and then isometric tension was measured.RESULTS: Both RES and EST caused the does-response contractile curve of KCl in K-H solution and CaCl_2 in Ca~(2+)-free K-H solution shift rightward,and their sensitivies and the values of maximum contractions had been decreased.In the N?-L-nitro-arginine,Methylene Blue and Sodium Orthovanadate groups,the vasorelaxant effects of both estrogens were markedly attenuated(P(0.05)).CONCLUSION: Resveratrol and 17?-Estradiol relaxe vascular smooth muscle in an endothelium-dependent manner,and are not related to KATP channel.

OBJECTIVE To sum up the cases of hospital infection,in order to further improve the experience of the work of hospital infection.METHODS The investigation of hospital infection situation among inpatients 2005-2007 was reviewed.RESULTS The hospital infection rates in 2005-2007 were 1.81%,1.65% and 1.52%,respectively,mainly in the lower respiratory tract infection and surgical incision.CONCLUSIONS Hospital infection is closely related with the age,the underlying diseases,the antibiotic misuse and the environment.

Objective:To study the effect of the association of ultrasound and microbubble with short hairpin RNA (shRNA) on the expression of survivin mRNA and protein in cervical cancer (HeLa) cell lines.Methods:Three survivin-shRNA expression vectors (pSIREN/S1/S2/S3) were constructed and introduced into HeLa cells.A corresponding negative control vector was constructed (pSIREN/con).The shRNA vectors were added to Hela cells transfected by LipofectamineTM (P+L) or followed by ultrasound exposure with SonoVue microbubble (US+SO).Blank control group,pSIREN/S1 plasmid only,ultrasound exposure only,pSIREN/S1 and ultrasound exposure were used as controls,respectively.The expressions of survivin mRNA and protein were detected by RT-PCR and western blot analysis,respectively.Results:Three recombinant plasmids were successfully constructed.The plasmids were showed to inhibit the expression of survivin gene mRNA and protein efficiently and specifically except for the pSIREN/con,while pSIREN/S3 was significantly lower than other groups (P

[Objective]To investigate the impact of endplate facture on prognosis of thoracolumbar fracture.[Method]Seventy-three patients with thoracolumbar fractures were treated with transpedicular bone grafting combined with pedicle screw fixation system.Of them,thirty-four cases eligible for the study were divided into groups A and B.Group A:no endplate fracture or minor endplate fracture.Group B:bursting endplate fracture.The height of fractured vertebra and the Cobb angle before and after operation,and at the final follow-up were determined and analysed.[Result]There were no significant differences in Cobb angles and height between growps A and B before operation.After operation,the height of fractured vertebra in group B were lower than that of group A.The Cobb angles had no significant difference between the two groups.The height and Cobb angles between the two groups had significant difference at final follow-up.The improvement in group A was significant higher than in group B.[Conclusion]Endplate bursting facture affects the reduction of fractured vertebra and causes loss of the vertebral height and the corrected angle.