Objective To observe the effects of Biejia Jian (BJ) in preventing rat hepatic fibrosis. Methods Rat models with hepatic fibrosis were established by abdominal subcutaneous injection of 45 %carbon tetrachloride (CCl4) and olive oil. Fifty Wistar rats were randomly divided into 4 groups: model group (treated with normal saline),small-dosage BJ group (6.25 g/kg BJ), moderate-dosage BJ group (12.5 g/kg BJ) and large-dosage BJ group (25.0 g/kg BJ). The treatment lasted 10 weeks. On the 11th week, rats eyes were removed to get blood sample. The biochemical parameters of blood were detected and HE staining was applied for the pathological examination of liver. Results BJ decreased the serum levels of ALT,AST,HA, LN and PC III in rats, no pseudo-lobules found in the BJ groups. Proliferation of fibrous tissue was mild and Limited and fatty degeneration of hepatocytes were slight in moderate-dosage and high-dosage BJ groups as compared with the small-dosage BJ group.Pathological changes in BJ groups were significantly improved (P

Objective This paper analyzes the situation of confidential blood discarding in voluntary blood donation in Jiaxing in 2011-2016 years,and provides scientific evidence for ensuring the blood safety.Methods During 2011-2016 years,15 voluntary blood donors who required confidential blood donation in the blood bank were investigated for their reasons and characteristics.Results The blood discarding rate was 0.011％.15 cases were abandoned in the confidential blood discarding of voluntary blood donation in Jiaxing,in which 14 blood donors' 4 infectious disease markers were negative,1 blood donor was HIV positive,18-30 years old unmarried males are the main category of secrecy abandoned blood donors,the number of secondary donors who have high-risk behaviors or diseases are relatively small.The causes of confidentiality were mainly high risk behaviors (27％) and feculent sexual history (20％).Conclusion Blood donors' conscience to report the truth is quite important to improve the safety of blood transfusion,it is a must to prepare the training of staffs,to broaden the back way,strengthen the propaganda and education,strengthen the consultation work before the blood donation and provide a private security environment of abandoned blood for blood donors.

Using immunohistochemical method,the antigens were shown to be mainly located in the cecum,tegument,esophagus,pharynx,excretory bladder,uterus and oral sucker of Pagumogonimus skrjabini.Histochemical analyses showed that antigens contained enzymes such as acid phosphatase (ACP),alkaline phosphatase (AKP),and acetyleholinesterase (AChE).Other chemical substances,for example,alkaline protein,basic protein,glycogen and acid mucopolysaccharides were also detected in the antigens of P.skrjabini.

OBJECTIVE To evaluate the bioethical principles which should be followed in the epidemiological study of AIDS.METHODS Practicality of bioethical principles in epidemiological study of AIDS was evaluated by theoretical analysis method.RESULTS The important principles in the epidemiological study of AIDS are respect,beneficence/nonmaleficence and justice principles.CONCLUSIONS Following bioehical principle is the basis of epidemiological study of AIDS.

Objective To investigate the changes of CRP in diagnosis of the acute exacerbation of COPD and its significance.Method CRP and WBC were measured both when the patients just before and after the treatment with effective antibiotics to compare their change.Results The positive rate of CRP is 85.40%.and the difference Was obvious(P＜0.01).At the same time,the CRP's difference between before and after the use of antibiotics was also obvious(P＜0.01).Conclusion CRP is a sensitive and specific index to the acute exacerbation of COPD,and is helpful to discover this acute disease early as well as a good guide to estimate the serious of pneumonia sand to respond the efficacy of antibiotics.

Background and purpose:The majority of patients with gastric cancer are in advanced stage with either extensive invasion of tumor into the adjacent organs,lymph nodes or distant metastases when diagnosed.As a new anticancer drug of Taxanes,docetaxel has shown considerable promise in advanced gastric cancer through clinical study in these years.We conducted a clinical trial to compare the response and toxic reaction of weekly and 3-weeks'docetaxel /cisplatin/5-Fu treatment in advanced gastric cancer.Methods:80 patients were deviede into two groups.Ggroup A:Docetaxel 25 mg/m2 iv d 1,8,15,cisplatin 25 mg/m2 iv d 1-3,5-FU 500 mg/m2 iv d 1-5 q4w.Group B:Docetaxel 75 mg/m2 iv d 1,cisplatin 25 mg/m2 iv d 1-3,5-FU 500 mg/m2 iv d 1-5 q3w;The clinical responses were assessed after two cycles.Toxicity was assessed every cycle.Results:There was 1 CR in 40 cases,16 with PR,14 with SD in group A;there was 1 CR in 40 cases,17 with PR,14 with SD in group B.The overall response rates were 42.4% and 45.0% in group A and B,respectively.Grade Ⅲ/Ⅳ neatropenia were 32.5% and 77.5% in group A and B.The major non-hematologic toxicity was weakness.Grade Ⅲ/Ⅳ weakness were 22.5% and 42.5% in group A and B.Conclusions:The response rates were similar between groups A and B.The occurrence of hematological toxicity and weakness were lower in weekly treatment group.

Expression of gamma interferon (IFN-?)-like substance in adrenal pheochromocytoma was investigated with immunohistochemical technique. In 15 out of the 16 cases of adrenal pheochromocytoma positive IFN-@-like immunoreactive substance was detected in the cytoplasm of the tumor cells, whereas in adrenal tissues adjacent to adrenal tumors in 26 cases (pheochromocytoma 12, adrenal corti-col tumors 12, others 2) only two with pheochromocytoma showed positive IFN-?-like immunoreactive substance in the cytoplasm of zona reticularis cells. The results suggest that IFN-? may be a kind of antigen associated with differentiation of cell.

AIM: To investigate the effect of homocysteine (Hcy) on secretion and expression of interleukin-6 (IL-6), which is a multifunctional proinflammatory cytokine, in cultured rat vascular smooth muscle cells (VSMCs). METHODS: Rat VSMCs were stimulated with Hcy. Cell ELISA was performed to measure the expression of IL-6 protein and semiquantitative RT-PCR was used to dectect the IL-6 mRNA expression. RESULTS: Compared with control, treatment of 0.25 mmol Hcy for 6 h could increase IL-6 production. In addition, Hcy concentration-dependently increased the expression of IL-6 protein in these cells. 0 1 mmol/L, 0 25 mmol/L Hcy increased IL-6 production 1 4-fold and 3 4-fold, respectively Furthermore, RT-PCR analysis demonstrated that homocysteine also enhanced IL-6 mRNA expression in a concentration- and time-dependent manner.CONCLUSION: Homocysteine can induce IL-6 expression in VSMCs and elicit vascular inflammatory response, which may thereby influence the pathogenesis of atherosclerosis.