Taxonomy of genus Rhodococcus has been changing since it was set up by Zopf in 1891 The classification of the genus has been changed dramatically in recent years, with the species being reclassified and new species described Th e changing of taxonomy is mainly on the methods' altering from old morphological to modern polyphasic taxonomy Additionally, there has been increased interest i n rhodococcus for its abilities to degrade a range of environmental pollutants a nd to transform or synthesize compounds with possible usefull application

Infection is one of the most serious diseases during the neonatal period and the leading cause of neonatal death.Early virus detection is particularly important in the vulnerable infants hospitalized in modern, family centered NICUs.These units bear increasing risks of virus transmission as they provide kangaroo care, 24 hour visiting policies, and are not only open to parents and siblings, but do also encourage their involvement.Due to the atypical clinical features, susceptibility to mixed viral-bacterial infection, lower positive rate of detection, non-specific treatment options, and causing short-term as well as long-term complications or even death if the infection is severe.Thus, it is very important to recognize respiratory virus infection early to prevent and control its spread in neonatal wards.This article aimed to elaborate the susceptibility factors, common pathogens, detection methods and the management of prevention, and control of neonatal respiratory virus infection.

Benzene ; analysis ; chemistry ; Benzene Derivatives ; blood ; urine ; Humans

Objective To summarize the experience of heart-lung transplantation.Methods Four patients with Eisenmenger’s syndrome underwent heart-lung homoplastic transplantation. All patients were complicated with severe pulmonary hypertension in New York Heart Association ( NYHA ) functional class IV. Cannulation for cardiopulmonary bypass consisted of a cannula in the high ascending aorta and separate vena caval cannulas. The heart-lung graft was moved into the chest, beginning with passage of the lung before the phrenic nerve pedicle. The bronchus was trimmed, leaving two cartilaginous rings proximal to the orifice of the upper lobe. The tracheal anastomosis was performed with a continuous 4-0 polypropylene suture, with the posterior portion continuously and anterior interrupted. The lungs were then ventilated (

[Objective]To investigate the impact of endplate facture on prognosis of thoracolumbar fracture.[Method]Seventy-three patients with thoracolumbar fractures were treated with transpedicular bone grafting combined with pedicle screw fixation system.Of them,thirty-four cases eligible for the study were divided into groups A and B.Group A:no endplate fracture or minor endplate fracture.Group B:bursting endplate fracture.The height of fractured vertebra and the Cobb angle before and after operation,and at the final follow-up were determined and analysed.[Result]There were no significant differences in Cobb angles and height between growps A and B before operation.After operation,the height of fractured vertebra in group B were lower than that of group A.The Cobb angles had no significant difference between the two groups.The height and Cobb angles between the two groups had significant difference at final follow-up.The improvement in group A was significant higher than in group B.[Conclusion]Endplate bursting facture affects the reduction of fractured vertebra and causes loss of the vertebral height and the corrected angle.

Objective To study the effect of suppressing metastasis between KAI1 and ME491 on pancreatic cancer.Methods Normal pancreases and pancreatic cancers were analyzed using Northern blot,in situ hybridization and immunohistochemistry.Results The expression of KAI1and ME491 was higher in pancreatic cancer than that in normal pancreases,KAI1 expression was higher in pancreatic cancer without metastasis than that in pancreatic cancer with metastasis(P0.05).Conclusion The gene of KAI1 may be involved in the metastasis of pancreatic cancer,ME491 gene may have no effect on the metastasis of pancreatic cancer.

Objective To evaluate the effect of recombinant expressing plasmid pCMV-KAI1 on the proliferative ability of PANC Ⅰ and MiaPaCa Ⅱ pancreatic cancer cells, and to observe the suppress metastatic mechanism of KAI1 gene in the malignancy.Methods The plasmid pCMV-KAI1 was transfected into the human pancreatic cancer cell lines PANC Ⅰ and MiaPaCa Ⅱ with liposome. The proliferative ability of these two pancreatic cancer cell lines were analyzed with MTT and colony-forming test, the cycle pattern was assayed by flow cytometry.Results After KAI1 expressing plasmid tranfected into PANC Ⅰ and MiaPaCa Ⅱ, the cell growth rates of the two cell lines were reduced by 40.59% and 65.84% respectively compared with the control cells (P

Objective To observe the changes of synaptosome membrane fluidity and intrasynaptosome free calcium in ovariectomized rats' brain and the therapeutical effects of genistein. Methods Thirty-six 3 month-old female Sprague-Dawley rats were randomly assigned to 4 groups:sham-operated,ovariectomized control,genistein and estradiol benzoate groups.Normal saline(50??l),normal saline(50??l),genistein(250??g)and estradiol benzoate (50??g)were subcutaneously injected respectively once every other day for 8 weeks,and then synaptosome membrane fluidity and intrasynaptosome free calcium in frontal and parietal lobe and hippocampus were detected. Results The intrasynaptosome free calcium of cerebral cortex and hippocampus synaptosomes of ovariectomized control group (?s)are (243.31?31.21)nmol/L and (305.10?54.31)nmol/L respectively.There are significantly statistical differences as compared ovariectomized control group with sham-operated,genistein and estradiol benzoate groups(P0.05).The synaptosome membrane viscosity(?)of hippocampal synaptosomes of ovariectomized control group(?s)is 3.03?0.39,which has significantly statistical differences compared with sham-operated and estradiol benzoate groups(P0.05).Conclusion The synaptosome membrane fluidity decreased while intrasynaptosome free calcium increased in ovariectomized rats' synaptosomes.Genistein can enhance the membrane fluidity and maintain intrasynaptosome free calcium in ovariectomized rats' synaptosomes.

Objective To evaluate the osteocompatibility of poly(D,L lactic acid)/ hydroxyapatite/decalcified bone matrix (PDLLA/HA/DBM) and to compare the osteocompatibility with that of PDLLA and DBM. Methods The isolated human osteoblasts from the femoral head of patients were inoculated onto PDLLA/HA/DBM, PLA, and DBM. The interface between biomaterial and osteoblasts was investigated with phase contrast microscope and scanning electron microscope. Results Osteoblasts were attached tightly to the surface of biomaterial with an arched structure and had normal morphology. The extracellular collagenous matrix covered the surface of biomaterial and packed the granules of biomaterial completely. Conclusion PDLLA/HA/DBM can form osteointegrated interface at the early stage with good biocompability.

Objective To study the effects of PDLLA/HA/DBMV biomaterials on cell proliferation by comparison of polyactic acid (PDLLA) and decalcified bone matrix (DBM) so as to evaluate the biocompatibility of the biomaterials at the cell level. Methods The isolated third generation human fetal osteoblasts (suspension, 3.5?10 5/ml) were inoculated onto the biomaterials of PDLLAHA/DBM, PDLLA, and DBM for 1, 2, 3, 5, and 7 d. Proliferation rates of cells on different materials were determined by flow cytometry. Results The osteoblast proliferation rate on PDLLA/HA/DBM was higher than that on PLA and DBM. Conclusion PDLLA/HA/DBM is compatible for the growth of osteoblasts.